Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase

Structure of cellobiose phosphorylase fromClostridium thermocellumin complex with phosphate

(2011) Christopher M. Bianchetti et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Engineering Escherichia coli Cells for Cellobiose Assimilation through a Phosphorolytic Mechanism

(2011) Ramanan Sekar et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Fusion of a family 9 cellulose-binding module improves catalytic potential of Clostridium thermocellum cellodextrin phosphorylase on insoluble cellulose

(2011) Xinhao Ye et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Substrate channeling and enzyme complexes for biotechnological applications

(2011) Y.-H. Percival Zhang   BIOTECHNOLOGY ADVANCES

Ultra-stable phosphoglucose isomerase through immobilization of cellulose-binding module-tagged thermophilic enzyme on low-cost high-capacity cellulosic adsorbent

(2011) Suwan Myung et al.   BIOTECHNOLOGY PROGRESS

Engineering of cellobiose phosphorylase for glycoside synthesis

(2011) Manu R.M. de Groeve et al.   JOURNAL OF BIOTECHNOLOGY

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Engineering of Clostridium phytofermentans Endoglucanase Cel5A for Improved Thermostability

(2010) W. Liu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum

(2010) Hiroyuki Nakai et al.   BIOCHIMIE

Beyond directed evolution—semi-rational protein engineering and design

(2010) Stefan Lutz   CURRENT OPINION IN BIOTECHNOLOGY

Protein engineering of microbial enzymes

(2010) Dominique Böttcher et al.   CURRENT OPINION IN MICROBIOLOGY

Thermophilic α-glucan phosphorylase from Clostridium thermocellum: Cloning, characterization and enhanced thermostability

(2010) Xinhao Ye et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Fast identification of thermostable beta-glucosidase mutants on cellobiose by a novel combinatorial selection/screening approach

(2009) Wenjin Liu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Spontaneous High-Yield Production of Hydrogen from Cellulosic Materials and Water Catalyzed by Enzyme Cocktails

(2009) Xinhao Ye et al.   ChemSusChem

Protein engineering in designing tailored enzymes and microorganisms for biofuels production

(2009) Fei Wen et al.   CURRENT OPINION IN BIOTECHNOLOGY

Directed enzyme evolution: climbing fitness peaks one amino acid at a time

(2009) Cara A Tracewell et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

In the light of directed evolution: Pathways of adaptive protein evolution

(2009) J. D. Bloom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Creating lactose phosphorylase enzymes by directed evolution of cellobiose phosphorylase

(2009) M. R.M. De Groeve et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage

(2008) Jiong Hong et al.   JOURNAL OF CHROMATOGRAPHY A

Increasing protein stability using a rational approach combining sequence homology and structural alignment: Stabilizing the WW domain

(2002) Xin Jiang et al.   PROTEIN SCIENCE