4.7 Article

Fungal diversity in cow, goat and ewe milk

Journal

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 151, Issue 2, Pages 247-251

Publisher

ELSEVIER
DOI: 10.1016/j.ijfoodmicro.2011.08.029

Keywords

Milk; Fungi; D-HPLC; Cow; Ewe; Goat

Funding

  1. French National Agency for Research (ANR) [ANR-09-ALIA-005-01]
  2. French Ministry of Higher Education and Research

Ask authors/readers for more resources

Knowledge of fungal diversity in the environment is poor compared with bacterial biodiversity. In this study, we applied the denaturing high-performance liquid chromatography (D-HPLC) technique, combined with the amplification of the ITS1 region from fungal rDNA, for the rapid identification of major fungal species in 9 raw milk samples from cow, ewe and goat, collected at different periods of the year. A total of 27 fungal species were identified. Yeast species belonged to Candida, Cryptococcus, Debaryomyces, Geotrichum, Kluyveromyces, Malassezia, Pichia, Rhodotorula and Trichosporon genera; and mold species belonged to Aspergillus, Chrysosporium, Cladosporium, Engyodontium, Fusarium, Penicillium and Torrubiella genera. Cow milk samples harbored the highest fungal diversity with a maximum of 15 species in a single sample, whereas a maximum of 4 and 6 different species were recovered in goat and ewe milk respectively. Commonly encountered genera in cow and goat milk were Geotrichum candidum, Kluyveromyces marxianus and Candida spp. (C catenulata and C. inconspicua); whereas Candida parapsilosis was frequently found in ewe milk samples. Most of detected species were previously described in literature data. A few species were uncultured fungi and others (Torrubiella and Malassezia) were described for the first time in milk (C) 2011 Elsevier B.V. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Food Science & Technology

Biogenic amine and antibiotic resistance profiles determined for lactic acid bacteria and a propionibacterium prior to use as antifungal bioprotective cultures

Monika Coton, Megane Lebreton, Marcia Leyva Salas, Lucille Garnier, Marion Navarri, Audrey Pawtowski, Gwenaelle Le Blay, Florence Valence, Emmanuel Coton, Jerome Mounier

INTERNATIONAL DAIRY JOURNAL (2018)

Article Biotechnology & Applied Microbiology

Protective Efficacy of a Pseudoalteromonas Strain in European Abalone, Haliotis tuberculata, Infected with Vibrio harveyi ORM4

Clement Offret, Vincent Rochard, Helene Laguerre, Jerome Mounier, Sylvain Huchette, Benjamin Brillet, Patrick Le Chevalier, Yannick Fleury

PROBIOTICS AND ANTIMICROBIAL PROTEINS (2019)

Article Microbiology

Antifungal Activity of Lactic Acid Bacteria Combinations in Dairy Mimicking Models and Their Potential as Bioprotective Cultures in Pilot Scale Applications

Marcia Leyva Salas, Anne Thierry, Mathilde Lemaitre, Gilles Garric, Marielle Harel-Oger, Manon Chatel, Sebastien Le, Jerome Mounier, Florence Valence, Emmanuel Coton

FRONTIERS IN MICROBIOLOGY (2018)

Article Biotechnology & Applied Microbiology

New insights into the haemo- and coelo-microbiota with antimicrobial activities from Echinodermata and Mollusca

C. Offret, C. Jegou, J. Mounier, Y. Fleury, P. Le Chevalier

JOURNAL OF APPLIED MICROBIOLOGY (2019)

Article Microbiology

Novel Antifungal Compounds, Spermine-Like and Short Cyclic Polylactates, Produced by Lactobacillus harbinensis K.V9.3.1Np in Yogurt

Amor Mosbah, Emilie Delavenne, Yasmine Souissi, Mouna Mahjoubi, Philippe Jehan, Nicolas Le Yondre, Ameur Cherif, Arnaud Bondon, Jerome Mounier, Michele Baudy-Floc'h, Gwenaelle Le Blay

FRONTIERS IN MICROBIOLOGY (2018)

Article Biotechnology & Applied Microbiology

Selection of Algerian lactic acid bacteria for use as antifungal bioprotective cultures and application in dairy and bakery products

Massinissa Ouiddir, Guessas Bettache, Marcia Leyva Salas, Audrey Pawtowski, Christelle Donot, Samira Brahimi, Kihel Mabrouk, Emmanuel Coton, Jerome Mounier

FOOD MICROBIOLOGY (2019)

Article Microbiology

Lactobacilli intra-tracheal administration protects from Pseudomonas aeruginosa pulmonary infection in mice - a proof of concept

M. S. Fangous, Y. Alexandre, N. Hymery, S. Gouriou, D. Arzur, G. Le Blay, R. Le Berre

BENEFICIAL MICROBES (2019)

Article Microbiology

The marine intertidal zone shapes oyster and clam digestive bacterial microbiota

Clement Offret, Sauvann Paulino, Olivier Gauthier, Kevin Chateau, Adeline Bidault, Charlotte Corporeau, Philippe Miner, Bruno Petton, Fabrice Pernet, Caroline Fabioux, Christine Paillard, Gwenaelle Le Blay

FEMS MICROBIOLOGY ECOLOGY (2020)

Article Biotechnology & Applied Microbiology

Mycobiota dynamics and mycotoxin detection in PGI Salame Piemonte

I. Franciosa, M. Coton, I. Ferrocino, M. R. Corvaglia, E. Poirier, J. -L. Jany, K. Rantsiou, L. Cocolin, J. Mounier

Summary: The study aimed to investigate fungal diversity and mycotoxin production during maturation of PGI Salame Piemonte. Penicillium nalgiovense and Debaryomyces hansenii were the most dominant taxa in casings, while a higher fungal diversity was observed in meat samples. Mycotoxins and penicillin G were monitored using QTOF LC-MS, and only trace amounts of roquefortine C were detected in two batches.

JOURNAL OF APPLIED MICROBIOLOGY (2021)

Article Food Science & Technology

Use of metabarcoding and source tracking to identify desirable or spoilage autochthonous microorganism sources during black olive fermentations

Marine Penland, Jerome Mounier, Audrey Pawtowski, Sylvie Treguer, Stephanie-Marie Deutsch, Monika Coton

Summary: This study investigated the influence of process environment and raw materials on microbial diversity during Nyons black table olive fermentations. Results showed significant differences in bacterial composition between control and spoiled fermentations, with higher diversity in the latter, especially for lactic acid bacteria. Fungal communities were similar, but process environment samples had higher diversity compared to fresh olive fruits. SourceTracker analysis highlighted the contribution of brine and water from the optical sorter as sources of fungi.

FOOD RESEARCH INTERNATIONAL (2021)

Article Food Science & Technology

Specific metagenomic asset drives the spontaneous fermentation of Italian sausages

Irene Franciosa, Ilario Ferrocino, Manuela Giordano, Jerome Mounier, Kalliopi Rantsiou, Luca Cocolin

Summary: Using Metagenomics, this study investigated the microbial dynamics in the fermentation process of Salame Piemonte sausage, identifying how different strains of L. sakei can significantly influence the quality characteristics of the final product.

FOOD RESEARCH INTERNATIONAL (2021)

Article Food Science & Technology

Tailor-made microbial consortium for Kombucha fermentation: Microbiota-induced biochemical changes and biofilm formation

Oceane Savary, Jerome Mounier, Anne Thierry, Elisabeth Poirier, Julie Jourdren, Marie-Bernadette Maillard, Marine Penland, Christophe Decamps, Emmanuel Coton, Monika Coton

Summary: A tailor-made microbial consortium representative of the core Kombucha microbiota was successfully used to drive the fermentation process. Organic acids such as acetic, lactic, succinic, and oxalic acids were produced, along with a gradual increase in gluconic and glucuronic acids during fermentation. The volatile profile also shifted throughout the fermentation process, with a variety of compounds identified at different stages.

FOOD RESEARCH INTERNATIONAL (2021)

Article Ecology

Microbiota of the Digestive Glands and Extrapallial Fluids of Clams Evolve Differently Over Time Depending on the Intertidal Position

Clement Offret, Olivier Gauthier, Garance Despreaux, Adeline Bidault, Charlotte Corporeau, Philippe Miner, Bruno Petton, Fabrice Pernet, Caroline Fabioux, Christine Paillard, Gwenaelle Le Blay

Summary: By studying the behavior of clam digestive glands and extrapallial fluids microbiota, this study found that environmental variations impacted clam microbiota differently according to the tissue considered. Clam tissues could be considered as different microhabitats for bacteria as they showed different responses to small-scale temporal and spatial variabilities in natural conditions.

MICROBIAL ECOLOGY (2023)

Article Biotechnology & Applied Microbiology

Mechanically Enhanced Salmo salar Gelatin by Enzymatic Cross-linking: Premise of a Bioinspired Material for Food Packaging, Cosmetics, and Biomedical Applications

Manon Buscaglia, Fabienne Guerard, Philippe Roquefort, Thierry Aubry, Marilyne Fauchon, Yannick Toueix, Valerie Stiger-Pouvreau, Claire Hellio, Gwenaelle Le Blay

Summary: Marine animal by-products, such as salmon skins and bones, are valuable sources of biomolecules, particularly collagen. Salmon gelatin, due to its high biomass production and biocompatibility, shows potential for various applications. However, its low mechanical and thermal properties can be overcome through enzymatic cross-linking using microbial transglutaminase (MTG). In this study, the potential of salmon skin gelatin was compared to porcine gelatin, and the effects of enzymatic and chemical cross-linking were analyzed. The results showed that enzymatically cross-linked gelatin had high cross-linking densities and improved mechanical and thermal resistances, making it a suitable candidate for biotechnological applications.

MARINE BIOTECHNOLOGY (2022)

Article Respiratory System

Porphyromonas, a potential predictive biomarker of Pseudomonas aeruginosa pulmonary infection in cystic fibrosis

Marlene Keravec, Jerome Mounier, Charles-Antoine Guilloux, Marie-Sarah Fangous, Stanislas Mondot, Sophie Vallet, Stephanie Gouriou, Rozenn Le Berre, Gilles Rault, Claude Ferec, Georges Barbier, Patricia Lepage, Genevieve Hery-Arnaud

BMJ OPEN RESPIRATORY RESEARCH (2019)

Article Food Science & Technology

Influence of sampling location and processing on the assembly and network of Polygoni Multiflori Radix surface microbiome

Guangfei Wei, Yichuan Liang, Guozhuang Zhang, Zhaoyu Zhang, Yongqing Zhang, Shilin Chen, Linlin Dong

Summary: This study investigated the presence of fungi on the surface of Polygonum multiflorum Thunb and found that it is easily contaminated by toxigenic fungi and mycotoxins, posing a health risk for consumers. The results also showed that sampling location and processing can influence the assembly and network complexity of the surface microbiome of Polygonum multiflorum Thunb.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

The wooden shelf surface and cheese rind mutually exchange microbiota during the traditional ripening process

Gabriele Busetta, Giuliana Garofalo, Salvatore Claps, Maria Teresa Sardina, Elena Franciosi, Antonio Alfonzo, Nicola Francesca, Giancarlo Moschetti, Luca Settanni, Raimondo Gaglio

Summary: This study evaluates the microbial diversity of wooden shelves used for the ripening of PDO Pecorino di Filiano and PGI Canestrato di Moliterno cheeses. The results show a strict correlation between the microbiota of wooden shelves and cheese rinds, indicating the positive role of wooden shelves in the cheese ripening process.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Reduction of PDO Pecorino Siciliano cheese making duration: Microbial dynamics and quality attributes deriving from replacing whey permeate with hot water during cooking

Massimo Todaro, Giuliana Garofalo, Gabriele Busetta, Riccardo Gannuscio, Ambra Rita Di Rosa, Maria Luisa Scatassa, Cinzia Cardamone, Isabella Mancuso, Elena Franciosi, Fatima Rando, Monica Agnolucci, Vincenzo Chiofalo, Raimondo Gaglio, Luca Settanni

Summary: This study aimed to reduce the production time of PDO Pecorino Siciliano cheese by comparing two cooking methods and assessing their impact on microbiological composition and lactic acid bacteria populations. The results showed that the different cooking methods did not affect the physicochemical characteristics of the cheese, and sensory evaluation confirmed that the modified production protocol did not significantly alter the product characteristics and overall acceptance. Therefore, cooking under hot water proved to be an effective way to reduce the transformation duration while maintaining the typicality of PDO Pecorino Siciliano cheese.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Effects of ohmic heating with different voltages on the quality and microbial diversity of cow milk during thermal treatment and subsequent cold storage

Yingying Sun, Yana Liu, Weiwei Zhou, Lele Shao, Han Wang, Yijie Zhao, Bo Zou, Xingmin Li, Ruitong Dai

Summary: The study evaluates the effect of Ohmic heating (OH) at various voltage gradients and water bath on microbial inactivation, physicochemical and sensory properties, and microbial flora of pasteurized milk. Results show that OH with higher voltage effectively inactivates microorganisms, slows down lipid oxidation, and maintains sensory quality and amino acids content of milk. The microbial community in milk treated with OH remains relatively stable during storage.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Bacteriophages PECP14, PECP20, and their endolysins as effective biocontrol agents for Escherichia coli O157:H7 and other foodborne pathogens

Minjin Oh, Ana Cevallos-Urena, Byoung Sik Kim

Summary: In this study, two bacteriophages, PECP14 and PECP20, infecting Escherichia coli O157:H7 were isolated and found to be stable under different environmental conditions. These phages exhibited specificity in targeting different host receptors and demonstrated the ability to infect other foodborne pathogens. The efficacy of phage treatment was demonstrated through rapid adsorption and bacterial lysis. Furthermore, purified endolysins derived from these phages showed remarkable bacteriolytic activity not only against E. coli O157:H7 but also against other pathogens. These findings suggest that these phages and their endolysins can be used for biological control of E. coli O157:H7 in various stages of agricultural product production.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Review Food Science & Technology

A systematic review on the occurrence of Salmonella in farmed Tenebrio molitor and Acheta domesticus or their derived products

Filippo Marzoli, Michela Bertola, Juliane Pinarelli Fazion, Giulia Cento, Pietro Antonelli, Beatrice Dolzan, Lisa Barco, Simone Belluco

Summary: Insects are a sustainable and protein-rich food source, and Salmonella is a common pathogen in insects. This systematic review aims to collect and evaluate existing studies on the occurrence of Salmonella in crickets and mealworms. Although the number of studies is limited and there are some limitations, this review highlights the importance of obtaining reliable data on the presence of Salmonella in insects, considering the growing market and increased farming scale.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Review Food Science & Technology

Comparison of Listeria monocytogenes alternative detection methods for food microbiology official controls in Europe

Lucas Chikhi, Melanie Mancier, Hubert Brugere, Bertrand Lombard, Lyazrhi Faouzi, Laurent Guillier, Nathalie Gnanou Besse

Summary: Listeriosis is a severe foodborne disease with a high mortality rate, mainly caused by L. monocytogenes. The EU regulation sets qualitative or quantitative food safety criteria for L. monocytogenes and recommends the use of EN ISO 11290-1 as the reference detection method. Our study compares alternative certified methods for detecting L. monocytogenes, highlighting the importance of diversifying available detection methods.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Ultralow temperature high pressure processing enhances inactivation of norovirus surrogates

Christina A. M. DeWitt, Kevin A. Nelson, Hyung Joo Kim, David H. Kingsley

Summary: High pressure processing (HPP) is a non-thermal method that can effectively inactivate pathogens. Research has shown that applying high pressure to thawed samples in an ultra-low temperature environment enhances the inactivation of norovirus.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Comparison of molecular techniques for the detection of Toxoplasma gondii in raw bovine milk from small rural properties in Brazil

Suzane Manzini, Thaina Valente Bertozzo, Isabella Neves Aires, Nassarah Jabur Lot Rodrigues, Amanda Bezerra Bertolini, Marcela Alexandrino, Jackieline Sampaio Steinle, Renata Pimentel Bandeira de Melo, Rinaldo Aparecido Mota, Maria Izabel Merino de Medeiros, Virginia Bodelao Richini-Pereira, Vera Claudia Lorenzetti Magalhaes Curci, Simone Baldini Lucheis

Summary: This study detected Toxoplasma gondii DNA in raw bovine milk samples from expansion tanks in small properties located in different cities of the Midwest region of Sa similar to o Paulo, Brazil using PCR. The results showed that the TgNP1 and TgNP2 genes were more efficient in detecting T. gondii DNA, highlighting the importance of raw bovine milk as a potential source of human infections caused by this parasite, and emphasizing the need for proper pasteurization and further understanding of the epidemiology of this protozoan.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Salmonella in horses at slaughter and public health effects in Italy

Luca Bolzoni, Mauro Conter, Luca Lamperti, Erika Scaltriti, Marina Morganti, Antonio Poeta, Marco Vecchi, Silvia Paglioli, Alessandra Rampini, Paolo Ramoni, Daniela De Vita, Cristina Bacci, Martina Rega, Laura Andriani, Stefano Pongolini, Silvia Bonardi

Summary: This study assessed the role of equids as carriers of Salmonella and the occurrence of contaminated equid carcasses during the slaughter process. Salmonella was detected in a small percentage of equids' caecal contents, but not in carcass samples. Additionally, a genetic match was found between Salmonella strains from horse caecal contents and human cases, suggesting a link between horse meat consumption and salmonellosis.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)

Article Food Science & Technology

Effect of multiple nonthermal plasma treatments of filamentous fungi on cellular phenotypic changes and phytopathogenicity

Daria Nowinski, Tomasz Czapka, Irena Maliszewska

Summary: The effect of multiple sublethal doses of non-thermal plasma treatments on phenotypical changes and the reduction in phytopathogenicity of three species of fungi was investigated. The plasma damaged the surface of the fungal hyphae, decreased their dry biomass, and affected the activity of pectinase and xylanase. Furthermore, the plasma treatments increased the germination rate of artificially infected cucumber seeds and resulted in plants with higher biomass and longer roots and stems. However, the reduction in phytopathogenicity was not complete after the multiple plasma treatments.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY (2024)