Article
Biochemistry & Molecular Biology
Hongliang Gao, Wei Zhang, Jing Zhang, Yimin Huang, Jingyu Zhang, Jiangtao Tian, Yanning Niu, Chunjing Zou, Caifeng Jia, Zhongyi Chang, Xuexia Yang, Deming Jiang
Summary: Curdlan, a water-insoluble exopolysaccharide produced by Agrobacterium species under nitrogen starvation, was significantly impaired in AmdeA, AmetA, AmetH, and AmetZ mutants. The impaired curdlan production was associated with down-regulation of genes involved in the electron transfer chain (ETC) and accumulation of ADP and FAD in the AmetH and AmetZ mutants. Addition of dibasic sodium succinate and methionine restored curdlan production in the mutants, indicating the importance of the methionine biosynthesis pathway in energy ATP supply during curdlan biosynthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Minjie Gao, Zhilei Liu, Zhongsheng Zhao, Zichao Wang, Xiuyu Hu, Yun Jiang, Jiajun Yan, Zhitao Li, Zhiyong Zheng, Xiaobei Zhan
Summary: This study explored the roles of exoR and exoX genes in curdlan biosynthesis in Agrobacterium sp. ATCC 31749. The results showed that the curdlan yields and glucose utilization increased in delta exoR and delta exoX strains compared with the wild-type strain during batch fermentation. However, the curdlan yields and gel strength decreased in exoR and exoX overexpression strains.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Minjie Gao, Guoshuai Yang, Feifei Li, Zichao Wang, Xiuyu Hu, Yun Jiang, Jiajun Yan, Zhitao Li, Xiaobei Zhan
Summary: This study involved the production of curdlan oligosaccharides by expressing endo-11-1,3-glucanase in Pichia pastoris and constructing a co-culture system. The optimized conditions in a 7-L bioreactor led to a maximum yield of 18.77 g/L of curdlan oligosaccharides with 3-10 degrees of polymerization, presenting a novel strategy for oligosaccharide production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Zhilei Liu, Ying Xu, Zichao Wang, Li Zhu, Zhitao Li, Yun Jiang, Xiaobei Zhan, Minjie Gao
Summary: During curdlan production, the encapsulation of Agrobacterium sp. by secreted exopolysaccharide (EPS) inhibits substrate uptake and curdlan synthesis. This study found that the addition of endo-ss-1,3-glucanase (BGN) effectively alleviated the EPS encapsulation effect and improved glucose consumption and curdlan yield. The upregulation of related genes and enhanced metabolic activity contributed to the increased production of curdlan.
CARBOHYDRATE POLYMERS
(2023)
Article
Biotechnology & Applied Microbiology
M. E. Morales-Plasencia, L. Ibarra-Castro, J. M. Martinez-Brown, M. Nieves-Soto, J. F. Bermudez-Lizarraga, A. H. Rojo-Cebreros
Summary: Nitrogen limitation significantly affects carbohydrate and beta-glucan accumulation in Nannochloropsis oculata. The highest biomass, carbohydrate, and beta-glucan content were achieved under 50% and 75% N treatments. It is recommended to conduct industrial production of N. oculata under these conditions.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Eldrin Dlr Arguelles, Milagrosa R. Martinez-Goss
Summary: Lipid-accumulating microalgae, such as Chlorolobion sp. and Chlorella sp., showed increased lipid yield and decreased biomass production under nitrogen-starved culture conditions. The obtained algal biodiesel exhibited high concentrations of saturated and monounsaturated fatty acid methyl esters, making them suitable for biodiesel production. Additionally, the biodiesel properties of the two microalgae met the set specifications of biodiesel standards EN 14214 (European) and ASTM D6751 (American), indicating their potential as raw materials for biodiesel production with superior fuel quality.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Microbiology
Wanaporn Tapingkae, Orranee Srinual, Chompunut Lumsangkul, Hien Van Doan, Hsin- Chiang, Atchara Manowattana, Pinpanit Boonchuay, Thanongsak Chaiyaso
Summary: Red yeast cells (RYCs) have been recognized as a potential feed additive due to their nutritional value and ability to bind mycotoxins. This study demonstrated the industrial feasibility of large-scale production of RYCs and tested their mycotoxin binding capacity.
Article
Biotechnology & Applied Microbiology
David Humbird
Summary: The analysis reveals that due to various limitations in animal cell production, the current costs of mass producing cultured meat products are high, making it difficult to replace conventional meat. Improving metabolic efficiency and developing low-cost plant hydrolysates as media do help address the issue, but there are still challenges ahead.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Microbiology
Hayk Palabikyan, Aquilla Ruddyard, Lara Pomper, David Novak, Barbara Reischl, Simon K. -M. R. Rittmann
Summary: This study provides a comprehensive revision and optimization of cultivation methods for Methanococcus maripaludis, a model organism for the production of methane biofuel. The research successfully achieved scale-up from serum bottles to a stainless steel bioreactor, contributing to the establishment of methanogens as essential organisms in large-scale biotechnology applications.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Mingcan Wu, Huan Qin, Jiuqi Deng, Yongqi Liu, Anping Lei, Hui Zhu, Zhangli Hu, Jiangxin Wang
Summary: This study utilized a new pilot-scale fermentation mode to evaluate the changes in E. gracilis biomass and paramylon production. The results showed enhanced cell viability under mixotrophic conditions and accumulation of paramylon under heterotrophic conditions, with maximal biomass and paramylon contents reaching 86.2 g/L and 78.2%, respectively. Metabolomics analysis revealed important pathways related to biomass and paramylon production, providing a valuable theoretical basis for industrial production of E. gracilis and other microalgae.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Chemistry, Physical
Jiangwei Chang, Chang Yu, Xuedan Song, Yiwang Ding, Siyi Hou, Shaofeng Li, Zongbin Zhao, Jieshan Qiu
Summary: This study presents an in situ framework reconstruction of g-C3N4 to achieve super nitrogen-doping, enhancing the catalytic activity of carbon materials. The results show the formation of nitrogen-doped graphene nanoribbons using ethene molecules as building blocks, with the nitrogen-doping level and configuration tunable. Using these super nitrogen-doped graphene nanoribbons as counter electrodes in dye-sensitized solar cells, a power conversion efficiency of 8.60% is achieved.
Article
Biotechnology & Applied Microbiology
Kerstin Rastaedter, David J. Wurm, Oliver Spadiut, Julian Quehenberger
Summary: This study analyzed and compared the influence of two different types of spargers on Pg/Vl and kla in a 2 L chemostat cultivation of S. acidocaldarius at 75 degrees C and a pH value of 3.0. The scale-up criterion kLa was then used to scale up to 20 L and 200 L. By maintaining a constant kla value, comparable dry cell weight, specific growth rate, specific substrate uptake rates, and viability were observed between all investigated scales. This procedure holds the potential for further scaling up to industrial-sized bioreactors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Environmental
Fahri Koray Sakarya, Berat Zeki Haznedaroglu, Ulas Tezel
Summary: Quaternary ammonium compounds (QACs) are key components in disinfectants used to combat the spread of SARS-CoV-2. This study demonstrates that a biocatalyst entrapped in calcium alginate beads can effectively degrade QACs, offering a cost-effective and robust treatment option for wastewater systems.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Fisheries
Summia Perveen, Lujia Yang, Suming Zhou, Bo Feng, Xiao Xie, Qicun Zhou, Dong Qian, Chunlin Wang, Fei Yin
Summary: The study showed that beta-1,3-glucans derived from Euglena gracilis significantly increased enzyme activities in crab hemocytes and up-regulated mRNA expression of immune-related genes, potentially stimulating innate immunity and providing antiparasitic activity in swimming crab farming.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Lucas J. Ustick, Alyse A. Larkin, Catherine A. Garcia, Nathan S. Garcia, Melissa L. Brock, Jenna A. Lee, Nicola A. Wiseman, J. Keith Moore, Adam C. Martiny
Summary: Nutrient supply affects phytoplankton activity, but understanding of global nutrient limitation and co-limitation is limited. Prochlorococcus adapt to local environments through gene gains and losses, with genomic changes indicating adaptation to nutrient stress. Regional transitions in stress type and severity, as well as widespread co-stress, were found, with correlations between Prochlorococcus stress genes, bottle experiments, and Earth system model predictions. The biogeography of multinutrient stress is linked through controls on nitrogen fixation, providing a detailed description of nutrient stress in the global ocean based on omics data.
Article
Biochemistry & Molecular Biology
Prasanth Ghanta, Shweta Sinha, Mukesh Doble, Basavaraju Ramaiah
Summary: Inflammation plays a crucial role in respiratory diseases. The alkaloids vasicine, vasicinone, and deoxyvasicine from Adhatoda vasica have bronchodilatory activity. The study found that vasicinone has potential as a competitive inhibitor, while vasicine and deoxyvasicine may act as allosteric inhibitors. These alkaloids could be potential leads for designing novel 5-LOX inhibitors.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Prasanth Ghanta, Mukesh Doble, Basavaraju Ramaiah
Summary: The study evaluated the potential of major alkaloids of A. vasica as inhibitors of COXs, with promising results showing that these alkaloids could be leads for the design of Eicosanoid pathway modulators.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biophysics
Ashok Kumar Shettihalli, Santosh Kumar Palanirajan, Sathyanarayana N. Gummadi
Summary: Lead and mercury, as common environmental pollutants, are often associated with erythrocytes, leading to PS exposure-mediated procoagulant activation. In the context of blood coagulation, cell activation, and apoptosis, hPLSCR1 plays a significant role, with higher binding affinity towards Pb2+ and Hg2+ contributing to their poisoning effects. The study demonstrates the involvement of cysteine residues as potential additional binding sites for heavy metal ions in hPLSCR1.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Gandarvakottai Senthilkumar Arumugam, Asmita Sen, Swati S. Dash, Kartik Mitra, Mukesh Doble, Gopalan Rajaraman, Sathyanarayana N. Gummadi
Summary: The ethanolic bark extract of Terminalia arjuna tree contains bio-active compound arjunetin, which exhibits inhibition of catalase activity and potential antiviral activity, particularly against SARS-CoV-2. Molecular docking and dynamics studies show that arjunetin has higher binding affinity than Remdesivir in targeting key proteins of the virus. These findings suggest that arjunetin could be a promising drug candidate against Covid-19.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Energy & Fuels
Rekha Rajesh, Sathyanarayana N. Gummadi
Summary: This study focuses on utilizing low-cost lignocellulosic substrates without pretreatment by a single novel Bacillus sp. PM06, which can produce dual enzymes, bioethanol, and acetic acid, making bioconversions more economically viable.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Water Resources
Divakar Raj Dinakaran, Hemaiswarya Shanmugam, Indumathi M. Nambi, Mukesh Doble
Summary: This study used uidA and lacZ genes as distinctive biomarkers for rapid and efficient detection of contaminated water resources. A total of 39 water samples from the metropolitan city of Chennai, India were collected and analyzed. The results showed that the performance and amplification efficiency of uidA and lacZ were 99.6% and 103.4% respectively. The qPCR method had a sensitivity of 71.4% in selectively identifying E. coli.
WATER PRACTICE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Amit Kumar, Sweta Mohan, Shatabdi Satpathy, Sharon Mano J. Pappu, Sathyanarayana N. Gummadi
Summary: This study investigates strategies to improve the production of xylitol in batch cultivation of Debaryomyces nepalensis. The results show that xylitol production is increased under limited initial phosphate concentration and by adding maltose. Addition of hydrogen peroxide and sodium hypochlorite as stress inducers also improves xylitol yields. Stress inducers and nutrient limitations could be considered for enhancing xylitol production in batch culture.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2022)
Article
Biotechnology & Applied Microbiology
M. K. Shanmugam, S. N. Gummadi
Summary: This study optimized induced cell concentration, aeration, and agitation rate to maximize caffeine degradation rate in a bioreactor. The maximum caffeine degradation rate achieved was 23.59 mg L-1 h(-1). A mathematical model was developed and experimental validation resulted in a caffeine degradation rate of 22 mg L-1 h(-1) under optimal conditions.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jerrina Pinto, Anju Chadha, Sathyanarayana N. Gummadi
Summary: Biocatalytic reduction catalysed by alcohol dehydrogenases is an important tool for synthesizing chiral alcohols. This study focuses on the broad substrate specificity of NADH dependent (S)-specific alcohol dehydrogenase (S-ADH) purified from Candida parapsilosis ATCC 7330. The results indicate that ketones and secondary alcohols are the most preferred substrates for S-ADH. The enzyme follows Prelog's stereopreference in reducing prochiral carbonyl substrates to yield (S)-alcohols with high enantiomeric excess using a simple coupled substrate approach for cofactor recycling.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Materials Science, Ceramics
Vimal Kumar Dewangan, T. S. Sampath Kumar, Mukesh Doble, Viju Daniel Varghese
Summary: A novel injectable macroporous apatite bone cement derived from eggshell was developed using gelatin and chitosan as a biopolymeric solution, along with disodium hydrogen phosphate and acetic acid. The cement exhibited good injectability, stability, and degradation properties, and showed potential for repairing low or non-load bearing defects in orthopaedic applications.
CERAMICS INTERNATIONAL
(2022)
Article
Biophysics
Sarika Rayala, Ulaganathan Sivagnanam, Sathyanarayana N. Gummadi
Summary: In this study, the DNA binding properties of human phospholipid scramblase 1 (hPLSCR1) and its different variants with IP3R1P oligonucleotide were investigated. It was found that hPLSCR1 and increment 100-hPLSCR1 bound to IP3R1P oligos in a 1:1 stoichiometry. Moreover, it was revealed that increment 100-hPLSCR1 possessed the minimal DNA binding region, while the binding of IP3R1P oligos to hPLSCR1 required its proline-rich N-terminal region.
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2022)
Article
Energy & Fuels
Yogeswar Mohanasundaram, Vishnu Damodaran Nambissan, Sathyanarayana N. N. Gummadi
Summary: A two-step sequential dilute alkali/acid pretreatment method was used to enhance the breakdown of lignocellulosic structure in corn cob. Uniform design and multiple regression models were employed to optimize the hydrolysis process. The alkali hydrolysis with 1.81% (w/v) NaOH concentration and 5 (w/v) solid:liquid ratio for 90 min resulted in 82.03% lignin removal. Subsequent dilute acid pretreatment with 6% (w/v) H2SO4 and 5 (w/v) solid:liquid ratio for 15 min yielded 74% xylose. The xylose-rich acid hydrolysate was utilized for xylitol production, achieving a xylitol yield of 0.467 g/g of xylose and a xylitol titer of 21.15 g/L. The physical and chemical structure changes of the corn cob due to the sequential alkali/acid pretreatment were analyzed using SEM, XRD, FTIR, and TGA.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Biotechnology & Applied Microbiology
S. Pavithra, Jayapriya Jayaprakash, Sathyanarayana N. Gummadi, Venkateshwarapuram Rengaswami Giri Dev
Summary: This study focuses on the integration of bioprocessing of agricultural residues and a natural fiber source (coir), which effectively produces lignin-modifying enzymes and softens the natural fibers. Results show that treating the fibers with Phanerochaete chrysosporium in the presence of agro waste can reduce the lignin content by 14-31%. Among the tested lignocellulosic wastes, coir fermented with groundnut husk produced the highest yield of lignin-modifying enzymes, while the tensile strength of the fiber decreased by 9.9%. The study demonstrates an efficient strategy of using low-cost agro residues for enzyme production and generating bio-softened coir for the market.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2023)
Article
Biochemistry & Molecular Biology
Sirisha Boddapati, Sathyanaryana N. Gummadi
Summary: A novel Cellulosimicrobium funkei strain SNG1 producing mutanase enzyme was isolated and optimized for increased productivity using the one-factor-at-a-time approach. The purified enzyme had a yield of 29.03% and a specific activity increase of 10.9-fold. The enzyme showed optimal pH and temperature at 6.0 and 45 degrees C, with a Km of 1.28 +/- 0.11 mg for alpha-1,3-glucans. The purified enzyme effectively degraded in vitro film with an 82.68% degradation rate and a saccharification yield of 30%.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Engineering, Environmental
Sasikaladevi Rathinavelu, Manoj Kumar Shanmugam, Sathyanarayana N. Gummadi, Indumathi M. Nambi
Summary: Antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are significant components of environmental antimicrobial resistance (AMR), and wastewater treatment plants contribute to the presence of AMR in the environment. Traditional tertiary treatment technologies are ineffective in disinfecting ARB or removing ARGs, highlighting the need to evaluate advanced wastewater treatment technologies. This study evaluated the potential of a Ti/Sb-SnO2/PbO2 anode in disinfecting ARB, inhibiting regrowth, removing intracellular and extracellular ARGs, and eliminating transformation efficiency.
CHEMICAL ENGINEERING JOURNAL
(2023)