A sensitive multidimensional method for the detection, characterization, and quantification of trace free drug species in antibody-drug conjugate samples using mass spectral detection

Direct Identification of Rituximab Main Isoforms and Subunit Analysis by Online Selective Comprehensive Two-Dimensional Liquid Chromatography–Mass Spectrometry

(2015) Dwight R. Stoll et al.   ANALYTICAL CHEMISTRY

A size exclusion-reversed phase two dimensional-liquid chromatography methodology for stability and small molecule related species in antibody drug conjugates

(2015) Yi Li et al.   JOURNAL OF CHROMATOGRAPHY A

Characterization of antibody drug conjugate positional isomers at cysteine residues by peptide mapping LC–MS analysis

(2015) Marie-Claire Janin-Bussat et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Utility of Ion Mobility Mass Spectrometry for Drug-to-Antibody Ratio Measurements in Antibody-Drug Conjugates

(2015) Richard Y.-C. Huang et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

A rapid on-line method for mass spectrometric confirmation of a cysteine-conjugated antibody-drug-conjugate structure using multidimensional chromatography

(2015) Robert E Birdsall et al.   mAbs

Characterization and Stability Study of Polysorbate 20 in Therapeutic Monoclonal Antibody Formulation by Multidimensional Ultrahigh-Performance Liquid Chromatography–Charged Aerosol Detection–Mass Spectrometry

(2014) Yi Li et al.   ANALYTICAL CHEMISTRY

Innovative Native MS Methodologies for Antibody Drug Conjugate Characterization: High Resolution Native MS and IM-MS for Average DAR and DAR Distribution Assessment

(2014) François Debaene et al.   ANALYTICAL CHEMISTRY

Limiting degradation of reactive antibody drug conjugate intermediates in HPLC method development

(2014) Yi Li et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Approaches to Interchain Cysteine-Linked ADC Characterization by Mass Spectrometry

(2014) John F. Valliere-Douglass et al.   MOLECULAR PHARMACEUTICS

Development of a Native Nanoelectrospray Mass Spectrometry Method for Determination of the Drug-to-Antibody Ratio of Antibody–Drug Conjugates

(2013) Jia Chen et al.   ANALYTICAL CHEMISTRY

Total Antibody Quantification for MMAE-Conjugated Antibody–Drug Conjugates: Impact of Assay Format and Reagents

(2013) Katherine R. Kozak et al.   BIOCONJUGATE CHEMISTRY

HPLC method development, validation and impurity characterization for an antitumor Hsp90 inhibitor—PU-H71 (NSC 750424)

(2013) Mingtao Liu et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Antibody-drug conjugates

(2013) Alain Beck et al.   mAbs

Antibody-drug conjugate model fast characterization by LC-MS following IdeS proteolytic digestion

(2013) Elsa Wagner-Rousset et al.   mAbs

Native Intact Mass Determination of Antibodies Conjugated with Monomethyl Auristatin E and F at Interchain Cysteine Residues

(2012) John F. Valliere-Douglass et al.   ANALYTICAL CHEMISTRY

On-line coupling of size exclusion chromatography with mixed-mode liquid chromatography for comprehensive profiling of biopharmaceutical drug product

(2012) Yan He et al.   JOURNAL OF CHROMATOGRAPHY A

Development and optimisation of an on-line solid phase extraction coupled to ultra-high-performance liquid chromatography–tandem mass spectrometry methodology for the simultaneous determination of endocrine disrupting compounds in wastewater samples

(2012) T. Vega-Morales et al.   JOURNAL OF CHROMATOGRAPHY A

Brentuximab Vedotin (SGN-35) in Patients With Relapsed or Refractory Systemic Anaplastic Large-Cell Lymphoma: Results of a Phase II Study

(2012) Barbara Pro et al.   JOURNAL OF CLINICAL ONCOLOGY

Conjugation site modulates the in vivo stability and therapeutic activity of antibody-drug conjugates

(2012) Ben-Quan Shen et al.   NATURE BIOTECHNOLOGY

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Analytical methods for physicochemical characterization of antibody drug conjugates

(2011) Aditya Wakankar et al.   mAbs

Challenges in developing bioanalytical assays for characterization of antibody–drug conjugates

(2011) Jean Philippe Stephan et al.   Bioanalysis

Immunotoxins and Anticancer Drug Conjugate Assemblies: The Role of the Linkage between Components

(2011) Franco Dosio et al.   Toxins

Molecularly Defined Antibody Conjugation through a Selenocysteine Interface

(2009) Thomas Hofer et al.   BIOCHEMISTRY

Targeting HER2-Positive Breast Cancer with Trastuzumab-DM1, an Antibody-Cytotoxic Drug Conjugate

(2008) G. D. Lewis Phillips et al.   CANCER RESEARCH

Quantitation of polysorbate 20 in protein solutions using mixed-mode chromatography and evaporative light scattering detection

(2008) Daniel Hewitt et al.   JOURNAL OF CHROMATOGRAPHY A

Separation of populations of antibody variants by fine tuning of hydrophobic-interaction chromatography operating conditions

(2008) John Valliere-Douglass et al.   JOURNAL OF CHROMATOGRAPHY A

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY

Targeted Cancer Therapy: Conferring Specificity to Cytotoxic Drugs

(2007) Ravi V. J. Chari   ACCOUNTS OF CHEMICAL RESEARCH

Comparison of the Practical Resolving Power of One- and Two-Dimensional High-Performance Liquid Chromatography Analysis of Metabolomic Samples

(2007) Dwight R. Stoll et al.   ANALYTICAL CHEMISTRY