Current Approaches for RNA Labeling in Vitro and in Cells Based on Click Reactions

Diels–Alder Cycloadditions on Synthetic RNA in Mammalian Cells

(2014) Anna Maria Pyka et al.   BIOCONJUGATE CHEMISTRY

Bioorthogonal site-specific labeling of the 5′-cap structure in eukaryotic mRNAs

(2014) Josephin Marie Holstein et al.   CHEMICAL COMMUNICATIONS

Programmable Design of Functional Ribonucleoprotein Complexes

(2014) Anna K. Rath et al.   Chemistry-An Asian Journal

Preparation of modified long-mer RNAs and analysis of FMN binding to theypaAaptamer fromB. subtilis

(2014) Jennifer Frommer et al.   RNA Biology

Visualization of Dynamics of Single Endogenous mRNA Labeled in Live Mouse

(2014) H. Y. Park et al.   SCIENCE

A Chemo-Enzymatic Approach for Site-Specific Modification of the RNA Cap

(2013) Daniela Schulz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Optimizing the selectivity of DIFO-based reagents for intracellular bioorthogonal applications

(2013) Eun J. Kim et al.   CARBOHYDRATE RESEARCH

Tetramolecular Fluorescence Complementation for Detection of Specific RNAs in Vitro

(2013) Stefanie Julia Kellermann et al.   CHEMBIOCHEM

Aqueous Oxidative Heck Reaction as a Protein-Labeling Strategy

(2013) Maria Eleni Ourailidou et al.   CHEMBIOCHEM

trans-Cyclooctene—a stable, voracious dienophile for bioorthogonal labeling

(2013) Ramajeyam Selvaraj et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A superfolding Spinach2 reveals the dynamic nature of trinucleotide repeat–containing RNA

(2013) Rita L Strack et al.   NATURE METHODS

Bioorthogonal labelling of biomolecules: new functional handles and ligation methods

(2013) Marjoke F. Debets et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

2′-Azido RNA, a Versatile Tool for Chemical Biology: Synthesis, X-ray Structure, siRNA Applications, Click Labeling

(2012) Katja Fauster et al.   ACS Chemical Biology

Fluorescent Probes for Imaging Endogenous β-Actin mRNA in Living Cells Using Fluorescent Protein-Tagged Pumilio

(2012) Hideaki Yoshimura et al.   ACS Chemical Biology

PNA FIT-Probes for the Dual Color Imaging of Two Viral mRNA Targets in Influenza H1N1 Infected Live Cells

(2012) Susann Kummer et al.   BIOCONJUGATE CHEMISTRY

Optimization of acetonitrile co-solvent and copper stoichiometry for pseudo-ligandless click chemistry with nucleic acids

(2012) Eduardo Paredes et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Chemical Reporters for Monitoring RNA Synthesis and Poly(A) Tail Dynamics

(2012) Markus Grammel et al.   CHEMBIOCHEM

Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry

(2012) Takumi Ishizuka et al.   CHEMICAL COMMUNICATIONS

A modified guanosine phosphoramidite for click functionalization of RNA on the sugar edge

(2012) Salifu Seidu-Larry et al.   CHEMICAL COMMUNICATIONS

An immobilized and reusable Cu(i) catalyst for metal ion-free conjugation of ligands to fully deprotected oligonucleotides through click reaction

(2012) Laxman Eltepu et al.   CHEMICAL COMMUNICATIONS

Profiling Genome-Wide Chromatin Methylation with Engineered Posttranslation Apparatus within Living Cells

(2012) Rui Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Programmable sequence-specific click-labeling of RNA using archaeal box C/D RNP methyltransferases

(2012) Miglė Tomkuvienė et al.   NUCLEIC ACIDS RESEARCH

Site-specific terminal and internal labeling of RNA by poly(A) polymerase tailing and copper-catalyzed or copper-free strain-promoted click chemistry

(2012) Marie-Luise Winz et al.   NUCLEIC ACIDS RESEARCH

From Mechanism to Mouse: A Tale of Two Bioorthogonal Reactions

(2011) Ellen M. Sletten et al.   ACCOUNTS OF CHEMICAL RESEARCH

Photoinducible Bioorthogonal Chemistry: A Spatiotemporally Controllable Tool to Visualize and Perturb Proteins in Live Cells

(2011) Reyna K. V. Lim et al.   ACCOUNTS OF CHEMICAL RESEARCH

Pin-point chemical modification of RNA with diverse molecules through the functionality transfer reaction and the copper-catalyzed azide–alkyne cycloaddition reaction

(2011) Kazumitsu Onizuka et al.   CHEMICAL COMMUNICATIONS

Inverse electron-demand Diels–Alder reactions for the selective and efficient labeling of RNA

(2011) Juliane Schoch et al.   CHEMICAL COMMUNICATIONS

Posttranscriptional chemical functionalization of azide-modified oligoribonucleotides by bioorthogonal click and Staudinger reactions

(2011) Harita Rao et al.   CHEMICAL COMMUNICATIONS

Versatile Site-Specific Conjugation of Small Molecules to siRNA Using Click Chemistry

(2011) Takeshi Yamada et al.   JOURNAL OF ORGANIC CHEMISTRY

Copper-Free Sonogashira Cross-Coupling for Functionalization of Alkyne-Encoded Proteins in Aqueous Medium and in Bacterial Cells

(2011) Nan Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Cellular Consequences of Copper Complexes Used To Catalyze Bioorthogonal Click Reactions

(2011) David C. Kennedy et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Labeling Substrates of Protein Arginine Methyltransferase with Engineered Enzymes and MatchedS-Adenosyl-l-methionine Analogues

(2011) Rui Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Diels–Alder Cycloaddition for Fluorophore Targeting to Specific Proteins inside Living Cells

(2011) Daniel S. Liu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A transgenic mouse for in vivo detection of endogenous labeled mRNA

(2011) Timothée Lionnet et al.   NATURE METHODS

RNA Mimics of Green Fluorescent Protein

(2011) J. S. Paige et al.   SCIENCE

A Metabolic Alkene Reporter for Spatiotemporally Controlled Imaging of Newly Synthesized Proteins in Mammalian Cells

(2010) Wenjiao Song et al.   ACS Chemical Biology

Enzymatic Site-Specific Functionalization of Protein Methyltransferase Substrates with Alkynes for Click Labeling

(2010) Wibke Peters et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Chemical Synthesis of Site-Specifically 2′-Azido-Modified RNA and Potential Applications for Bioconjugation and RNA Interference

(2010) Michaela Aigner et al.   CHEMBIOCHEM

Click Chemistry for Rapid Labeling and Ligation of RNA

(2010) Eduardo Paredes et al.   CHEMBIOCHEM

Live-Cell Imaging of Cellular Proteins by a Strain-Promoted Azide-Alkyne Cycloaddition

(2010) Kimberly E. Beatty et al.   CHEMBIOCHEM

Expanding the chemical scope of RNA:methyltransferases to site-specific alkynylation of RNA for click labeling

(2010) Y. Motorin et al.   NUCLEIC ACIDS RESEARCH

New strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes

(2010) A. H. El-Sagheer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Copper-free click chemistry in living animals

(2010) P. V. Chang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Analysis and Optimization of Copper-Catalyzed Azide–Alkyne Cycloaddition for Bioconjugation

(2009) Vu Hong et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Exploring Chemical Modifications for siRNA Therapeutics: A Structural and Functional Outlook

(2009) Siddharth Shukla et al.   ChemMedChem

Selective Functionalization of a Genetically Encoded Alkene-Containing Protein via “Photoclick Chemistry” in Bacterial Cells

(2008) Wenjiao Song et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Transition States of Strain-Promoted Metal-Free Click Chemistry:  1,3-Dipolar Cycloadditions of Phenyl Azide and Cyclooctynes

(2008) Daniel H. Ess et al.   ORGANIC LETTERS

Exploring RNA transcription and turnover in vivo by using click chemistry

(2008) C. Y. Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conjugation of Nucleosides and Oligonucleotides by [3+2] Cycloaddition

(2007) Anup M. Jawalekar et al.   JOURNAL OF ORGANIC CHEMISTRY