Article
Biochemistry & Molecular Biology
Janson E. Hoeher, Natalie E. Sande, Julia R. Widom
Summary: Riboswitches are mRNA segments that regulate gene expression in response to ligand binding. In this study, the insertion of 2-aminopurine (2-AP) into the riboswitch revealed how specific adenine residues impact the riboswitch and decrease its affinity for preQ1. The results highlight the critical role of A residues in ligand recognition and the complex effects of 2-AP substitution on the riboswitch.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2023)
Article
Chemistry, Organic
Hinano Takemura, Gaku Orimoto, Akihiro Kobayashi, Takamitsu Hosoya, Suguru Yoshida
Summary: This study discloses assembling methods using 2-azidoacrylamides with a nucleophilic amino group. Divergent transformations of the amine-type trivalent platform resulted in a wide range of 2-azidoacrylamides involving a fluorosulfonyl group. Consecutive click conjugations, including triazole formation, thiol-ene-type 1,4-addition, and SuFEx reactions, efficiently assembled easily available simple modules.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Krishna C. Suddala, Janghyun Yoo, Lixin Fan, Xiaobing Zuo, Yun-Xing Wang, Hoi Sung Chung, Jinwei Zhang
Summary: T-box riboswitch RNAs directly bind to specific tRNA and regulate the transcription or translation of downstream genes in bacteria. Using single-molecule FRET and ensemble biophysical analyses, here the authors uncover a Venus flytrap-like mechanism where tRNA binding to a T-box riboswitch mRNA triggers its rapid domain closure.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Anthony J. Rojas, Justin M. Wolfe, Heemal H. Dhanjee, Ivan Buslov, Nicholas L. Truex, Richard Y. Liu, Walter Massefski, Bradley L. Pentelute, Stephen L. Buchwald
Summary: The synthesis of palladium oxidative addition complexes derived from unprotected peptides is described. These complexes can react with thiols in aqueous buffer, providing an efficient method for bioconjugation. Using this strategy, peptides can be functionalized with small molecules to prepare modified aryl thioether side-chains at low micromolar concentrations. Additionally, peptide-peptide and peptide-protein ligations are demonstrated under dilute aqueous conditions.
Article
Chemistry, Multidisciplinary
Sandeep Kumar, Sumit Kumar, Jyotirmoy Maity, Banty Kumar, Shilpika Bali Mehta, Ashok K. Prasad
Summary: The Groebke-Blackburn-Bienayame (GBB) reaction is efficiently used for synthesizing novel fluorescent 5-azaindolizino-2'-deoxyuridines from commercially available thymidine. These synthesized compounds show strong fluorescence properties and have potential applications in studying the local structure and dynamics of nucleic acids.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jamie Richards, Joel G. Belasco
Summary: Riboswitches are known to modulate transcription elongation or translation initiation, but a study on guanidine III riboswitch reveals that ligand binding can also protect mRNA from degradation by creating a structure that impedes scanning by regulatory endonuclease RNase E. This finding suggests that riboswitch aptamers can independently regulate gene expression by prolonging mRNA longevity in response to ligand binding.
Article
Chemistry, Multidisciplinary
Olga A. Krasheninina, Julia Thaler, Matthias D. Erlacher, Ronald Micura
Summary: This study introduces a new method for metal-free diazotransfer on native RNA, which can be used for tagging and developing other RNA tools. The azido-RNA obtained by this method can be further processed through well-established bioorthogonal reactions, expanding its applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemical Research Methods
Alexandra Bristiel, Manon Cadinot, Mathieu Pizzonero, Frederic Taran, Dominique Urban, Raphael Guignard, Dominique Guianvarc'h
Summary: The researchers developed a facile method for conjugating relevant probes, ligands, or delivery agents onto oligonucleotides (ONs), which is important for both fundamental studies in chemical biology and improving the pharmacology of ONs in medicinal chemistry. They introduced novel 2'-functionalized nucleoside phosphoramidite monomers that contain bioorthogonal methylcyclopropene or sydnone moieties for the first time to ON solid-phase synthesis, allowing traceless ON postsynthetic modifications with reactive complementary probes through inverse electron-demand Diels-Alder reactions or strain-promoted sydnone-alkyne cycloaddition. These findings expand the bioorthogonal phosphoramidite building blocks and provide a new method for generating labeled ONs.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Sumirtha Balaratnam, Curran Rhodes, Desta Doro Bume, Colleen Connelly, Christopher C. Lai, James A. Kelley, Kamyar Yazdani, Philip J. Homan, Danny Incarnato, Tomoyuki Numata, John S. Schneekloth
Summary: This study investigates the modification ability of various PreQ(1)-derived probes on the riboswitch aptamer, revealing successful binding and retention of activity for one probe. Transcriptome-wide mapping shows highly selective interaction between the bacterial aptamer and the probe, with evidence of possible PreQ(1) aptamers in human RNA.
NATURE COMMUNICATIONS
(2021)
Article
Polymer Science
Zihao Li, Han Zhang, Xinqi Zhang, Jiaxi Wang, Yeqian Wen
Summary: In this study, four new polysulfonates with cyano substitution in the branched chain were successfully synthesized through a one-pot reaction. The synthesized polysulfonates exhibited excellent thermal stability and high glass transition temperature. This convenient synthesis method provides a new pathway for the synthesis of polysulfonates.
Article
Multidisciplinary Sciences
Xiao-Jing Zhang, Zhuo Zhao, Xia Wang, Min-Hui Su, Lili Ai, Yingying Li, Quan Yuan, Xue-Qiang Wang, Weihong Tan
Summary: A new chemical tailoring strategy was developed to efficiently produce circular bivalent aptamers and cyclized DNA sequences. These modified molecules exhibited high nuclease resistance and significantly improved binding, targeting, and tumor tissue enrichment abilities, thus showing great potential for biomedical applications.
NATIONAL SCIENCE REVIEW
(2023)
Article
Chemistry, Inorganic & Nuclear
Changhyeon Seo, Yeon-Joo Cheong, Woojin Yoon, Jaegyeom Kim, Jinho Shin, Hoseop Yun, Seung-Joo Kim, Hye-Young Jang
Summary: Mononuclear copper complexes modified with tridentate tris(N-heterocyclic carbene) (triscarbene) ligands were synthesized and characterized using various analytical techniques. These complexes were then utilized for the cycloaddition of alkynes and azides, with kinetic study results presented.
Article
Biochemistry & Molecular Biology
Dorothee Ganz, Philipp Geng, Hans-Achim Wagenknecht
Summary: Four different 2'-deoxyuridines were synthesized and reacted with three different dienophiles. The reactivity of inverse electron demand Diels-Alder reaction increased with the increasing ring strain of the dienophiles. The fluorescence turn-on ratios of the resulting fluorogenic tetrazinemodified cyanine-styryl dye were suitable for washfree cellular imaging. The incorporation efficiency of the modified 2'-deoxyuridines into cellular DNA was influenced by the steric demand of the dienophiles and an optimized way of cell culturing allowed even larger dienophiles to be incorporated.
ACS CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Organic
Cong Guan, Jiabin Yin, Jian Ji, Jinhua Liu, Xiang Wu, Tong Zhu, Shunying Liu
Summary: A regioselective electrochemical C-H amination method using easily accessible ethers has been developed to synthesize N (2)-substituted 1,2,3-triazole. Multiple substituents, including heterocycles, were well-tolerated, and 24 examples were obtained in moderate to good yields. Control experiments and DFT calculations revealed that the electrochemical synthesis occurs through a N-tosyl 1,2,3-triazole radical cation process, promoted by single-electron transfer from the lone pair electrons of the aromatic N-heterocycle, and the desulfonation is responsible for the high N (2)-regioselectivity.
Article
Chemistry, Analytical
Zhiqun Xu, Zhexiang Zhang, Zongkang She, Chenchen Lin, Xucong Lin, Zenghong Xie
Summary: A novel aptamer-functionalized metal-organic framework nanofibrous composite was proposed for the specific affinity recognition of trace Microcystin-LR (MC-LR), achieving good selective and sensitive recognition with a low detection limit. The composite demonstrated satisfactory stability, reproducibility, and recovery rates for MC-LR in environmental water samples, showing promise for ultra-trace MC-LR detection with good selectivity and resolution.
Article
Chemistry, Multidisciplinary
Megane Debiais, Amandine Lelievre, Jean-Jacques Vasseur, Sabine Mueller, Michael Smietana
Summary: The 10-23 DNAzyme is a Mg2+-dependent catalytic oligonucleotide that can cleave RNA in a sequence-specific manner. In this study, new split 10-23 DNAzymes were designed using nonfunctional fragments that, when assembled with Mg2+ ions, form boronate internucleoside linkages to restore DNAzyme activity. The best-performing system was identified through systematic evaluation, highlighting key features for controlling DNAzyme activity via boronate linkages.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Gastroenterology & Hepatology
Ujjwal M. Mahajan, Qi Li, Ahmed Alnatsha, Jessica Maas, Michael Orth, Sebastian Hubertus Maier, Julian Peterhansl, Ivonne Regel, Matthias Sendler, Preshit R. Wagh, Neha Mishra, Yonggan Xue, Prince Allawadhi, Georg Beyer, Jens-Peter Kuehn, Thomas Marshall, Bettina Appel, Felix Laemmerhirt, Claus Belka, Sabine Mueller, Frank-Ulrich Weiss, Kirsten Lauber, Markus M. Lerch, Julia Mayerle
Summary: The use of EGFR aptamers as carriers for targeted delivery of 5FU to pancreatic ductal adenocarcinoma cells has shown promising results, effectively reducing tumor growth and overcoming 5FU resistance. This approach has the potential to target various cancer entities sensitive to 5FU treatment.
Article
Virology
Jackie E. Mahar, Maria Jenckel, Nina Huang, Elena Smertina, Edward C. Holmes, Tanja Strive, Robyn N. Hall
Summary: In recent years, the diversity of lagoviruses in Australia has significantly increased, with the emergence of new recombinant variants. The new recombinant variants replaced the previous dominant strains in their respective geographical areas, highlighting the importance of recombination in lagovirus evolution.
Review
Chemistry, Multidisciplinary
Frederik Mueggenburg, Sabine Mueller
Summary: This review summarizes the synthetic methods of azide-modified nucleosides and their applications in RNA and DNA functionalization.
Article
Biochemistry & Molecular Biology
Huan Peng, Amandine Lelievre, Katharina Landenfeld, Sabine Muller, Irene A. Chen
Summary: Encapsulation enhances RNA activity and evolutionary rate by promoting RNA-RNA association and stabilizing tertiary structures, which facilitates the formation of more complex structures and cooperative interactions, indicating its significance in the origin of life and construction of synthetic cells.
Article
Biochemistry & Molecular Biology
Robert Hieronymus, Jikang Zhu, Sabine Mueller
Summary: This study demonstrates that small RNAs capable of self-cleavage and ligation may have been precursors for more complex self-splicing group I and II introns in an early RNA world. Engineered hairpin ribozyme variants, functioning as self-splicing introns, were shown to undergo two cleavage reactions at intron-exon junctions followed by ligation of exon fragments, leading to the removal of the ribozyme. Two self-splicing hairpin ribozyme variants, one designed manually and the other using a computer-aided approach, were found to efficiently perform self-splicing.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Maria Jenckel, Ina Smith, Tegan King, Peter West, Patrick L. Taggart, Tanja Strive, Robyn N. Hall
Summary: In 2020, Hepatitis E virus (HEV) was detected for the first time in Australian rabbits, showing a wide distribution and genetic diversity in rabbit populations across Australia. Phylogenetic analyses revealed that Australian HEV sequences are genetically diverse and were likely introduced independently multiple times.
Article
Infectious Diseases
Maria Jenckel, Robyn N. Hall, Tanja Strive
Summary: Australia is known for using biocontrol agents to manage wild rabbit populations. This study found that besides myxoma virus and rabbit haemorrhagic disease virus, other pathogens such as Clostridia, Pasteurella multocida, Pseudomonas spp., Eimeria stiedae, Hepatitis E virus, and Cyniclomyces yeast could contribute to rabbit mortality. Metatranscriptomic sequencing and host transcriptome analysis provided insights to distinguish between pathogenic microbes and non-infectious causes. Although no new rabbit pathogens were identified, this study provides a robust workflow for future investigations into rabbit mortality events.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Oncology
Hannes Ahrend, Bettina Appel, Lisa Jung, Sabine Mueller, Matthias B. Stope
Summary: This study evaluates the potential of using synthetic chemically modified miR molecules as anticancer drugs. The research found that the biological activity of synthetic miR-1 can be enhanced by modifying the C2' -OH group, providing a new approach for the development of multi-targeting nucleic acid-based drugs for cancer therapy.
ANTICANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jikang Zhu, Robert Hieronymus, Sabine Mueller
Summary: The majority of RNA splicing in organisms today involves the regulated removal of introns from pre-mRNAs. A model of earlier life forms suggests that RNA splicing could have occurred by a hairpin ribozyme derived spliceozyme that cuts out introns and joins exons. The excised intron then acts as a positive regulator for a DNAzyme. This study demonstrates how small RNAs can perform complex RNA processing and deliver functional RNA molecules.
Article
Chemistry, Multidisciplinary
Amandine Lelievre-Buettner, Theodor Schnarr, Megane Debiais, Michael Smietana, Sabine Mueller
Summary: The researchers demonstrate that boronate esters can replace natural phosphodiester linkages in functional RNA molecules, allowing for the assembly and restoration of their functionality.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Alexander Hautke, Arthur Voronin, Fathia Idiris, Anton Riel, Felix Lindner, Amandine Lelievre-Buttner, Jikang Zhu, Bettina Appel, Edoardo Fatti, Karsten Weis, Sabine Muller, Alexander Schug, Simon Ebbinghaus
Summary: A hallmark of Huntington's disease is a prolonged polyglutamine sequence in the huntingtin protein and an expanded CAG triplet repeat region in the mRNA. The RNA foci and therapeutic approaches targeting the mRNA have attracted attention. Through experiments, it was found that CAG-RNA is destabilized in cells but remains folded in the cytoplasm and nucleus. ATP plays a crucial role in promoting unfolding and affecting CAG-RNA homeostasis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Christoph Kaiser, Marc Vogel, Bettina Appel, Julia Weigand, Sabine Mueller, Beatrix Suess, Josef Wachtveitl
Summary: Engineering in vitro selected RNA aptamers into in vivo functional riboswitches is a challenge in molecular biology. Magnesium ions play a crucial role in the folding process.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Michael Smietana, Sabine Mueller
Summary: Boronate esters, with their reversible formation and ability to control the assembly of functional nucleic acid architectures, have implications in the origin of life and potential applications in biosensing. Stimuli-responsive boronate ester formation offers significant advantages in controlling the assemblies of functional structures derived from DNAzymes, ribozymes, and aptamers.
Article
Chemistry, Organic
Megane Debiais, Alejandro Gimenez Molina, Sabine Muller, Jean-Jacques Vasseur, Ivan Barvik, Carine Baraguey, Michael Smietana
Summary: Inspired by boronic acids' ability to bind with diol-containing compounds, we successfully formed boronate ester-based macrocycles through assembly in solution, and investigated their dynamic assembly and sugar pucker conformation profile.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)