Article
Biochemistry & Molecular Biology
Wenqi Feng, Hongwanjun Zhang, Yang Cao, Cheng Yang, Muhammad Hayder Bin Khalid, Qingqing Yang, Wanchen Li, Yingge Wang, Fengling Fu, Haoqiang Yu
Summary: This study identified and classified 19 ZmPum genes in maize and found that they exhibit diversity in gene and protein architectures, physicochemical properties, and expression patterns. The research also revealed the involvement of ZmPum genes in kernel development, with certain genes showing significantly high expression levels during seed development. These findings provide valuable information for understanding the functions of ZmPum genes and offer insights for improving maize yield.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sung Un Huh
Summary: Plant Pum proteins play important roles in defense and responses to biotic/abiotic stresses by specifically binding to the 3' UTR of target genes, controlling gene expression in a sequence-specific manner and rRNA processing in a sequence-nonspecific manner.
Article
Oncology
Isabelle Leticia Zaboroski Silva, Arissa Andreina Kohata, Patricia Shigunov
Summary: Post-transcriptional regulation involving RNA-binding proteins such as Pumilio plays an important role in tumorigenesis. Pumilio 1 and Pumilio 2 have distinct roles in cancer, and their dysregulation is associated with tumor progression and poor prognosis in certain types of cancer. The expression and function of Pumilio proteins are regulated by a variety of modulators, including miRNAs, lncRNAs, and circRNAs.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xin Li, Mengyi Zhu, Min Zang, Dandan Cao, Zhengyao Xie, Haibo Liang, Zexin Bian, Tingting Zhao, Zhibin Hu, Eugene Yujun Xu
Summary: The study highlights the essential role of PUM1 in regulating cell cycle progression in ovarian somatic cells, which is crucial for follicular development and female fertility. PUM1 targets key cell cycle regulators and its loss leads to reduced granulosa cell proliferation, affecting follicular growth and fertility. This translational control mechanism mediated by PUM1 is implicated in mammalian female reproduction and may have implications in human cancer biology.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Oncology
Xun-Xi Lu, Wen-Xiao Yang, Yu -Chen Pei, Hong Luo, Xiao-Guang Li, Yun-Jin Wang, Guo-Liang Zhang, Hong Ling, Zhi-Ming Shao, Xin Hu
Summary: Dysregulation of RNA-binding proteins (RBPs) is a characteristic of cancer. Investigating the functions and mechanisms of abnormal RBPs can help identify new cancer biomarkers and treatment strategies. In this study, SNRPC was identified as an oncogenic modulator in triple-negative breast cancer (TNBC). SNRPC upregulation was associated with poor prognosis, and its inhibition impaired TNBC cell proliferation, migration, and invasion. SNRPC also contributed to the stability of U1 snRNP and the transcriptional program controlled by RNA Pol II. Knockdown of SNRPC reduced the expression of oncogenes and inhibited TNBC progression via the TNFAIP2-Rac1-β-catenin signaling pathway.
Article
Biochemistry & Molecular Biology
Henri A. Galez, Francoise M. Roelants, Sarah M. Palm, Kendra K. Reynaud, Nicholas T. Ingolia, Jeremy Thorner
Summary: The study demonstrates that Puf1 and Puf2 are substrates of Ypk1, and their phosphorylation promotes the production of protein products from bound transcripts, thereby influencing the content of plasma membrane-associated proteins and cell membrane homeostasis.
Article
Biochemistry & Molecular Biology
Xiao Wu, Jian-Hui Li, Long Xu, Ya-Xiong Li, Xiao-Xu Zhu, Xi-Yu Wang, Xingmei Wu, Wei Zhao, Xuhao Ni, Xiao-Yu Yin
Summary: This study identified SENP3 as a potential suppressor of PDAC progression through its interaction with DKC1 and catalyzing the deSUMOylation of DKC1. Overexpression of DKC1 counteracted the anti-metastasis effect of SENP3, and elevated levels of DKC1 were associated with a poor prognosis in PDAC patients.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Plant Sciences
M. J. Nishanth, Bindu Simon
Summary: This study is the first to report the potential influence of Pumilio (Pum) RNA binding proteins on TIA biosynthesis in C. roseus. Knockdown of CrPum5 was found to increase TIA levels and upregulate key pathway genes.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2022)
Article
Biochemistry & Molecular Biology
Christophe Bignon, Sonia Longhi
Summary: This article introduces a method using the split-green fluorescent protein (GFP) reassembly assay to study protein-protein interactions. Two interacting proteins X and Y, fused to different parts of GFP, are co-expressed in E. coli to recreate functional GFP. This assay can be used as a potential tool for screening binding inhibitors.
Article
Multidisciplinary Sciences
Anna Kuchina, Leandra M. Brettner, Luana Paleologu, Charles M. Roco, Alexander B. Rosenberg, Alberto Carignano, Ryan Kibler, Matthew Hirano, R. William DePaolo, Georg Seelig
Summary: microSPLiT is a high-throughput single-cell RNA sequencing method that can resolve heterogeneous transcriptional states in Gram-negative and Gram-positive bacteria. Researchers used microSPLiT to process Bacillus subtilis cells and obtained detailed information on changes in metabolism and lifestyle, as well as identified new gene expression states in the bacterial population.
Article
Cell Biology
Yuchao Diao, Yingwei Li, Zixiang Wang, Shourong Wang, Peng Li, Beihua Kong
Summary: Studies have shown that overexpression of SF3B4 in ovarian cancer patients is associated with poor prognosis, and miR-509-3p negatively regulates SF3B4 expression which in turn promotes ovarian cancer progression. Additionally, SF3B4's splicing of RAD52 also impacts the development of ovarian cancer.
CELL DEATH & DISEASE
(2022)
Article
Agronomy
Jinfeng Hua, Chao Pan, Yongmei Huang, Yanqing Li, Huifeng Li, Cuirong Wu, Tianyuan Chen, Daifu Ma, Zongyun Li
Summary: The study found that CforOBP1 is involved in the process of weevils feeding and finding sweet potatoes, while CforOBP2 and CforOBP3 are mainly involved in the mating behavior of adult male weevils.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemical Research Methods
Nien-Chen Huang, Kai-Ren Luo, Tien-Shin Yu
Summary: We developed a background-free RNA live-cell imaging system by using a split fluorescent protein-based approach. Our system successfully eliminates background fluorescence in the nucleus and enables visualization of subcellular mRNA distribution in living plant cells.
Article
Biochemistry & Molecular Biology
Satyajit Patra, Jean-Benoit Claude, Jean-Valere Naubron, Jerome Wenger
Summary: G-quadruplexes (GQs) are a non-canonical form of DNA that have potential applications in antiviral and anticancer drug treatments. The interaction between RGG and GQs is predominantly driven by electrostatics, but also maintains a specific affinity between the RGG sequence and the GQs structure.Single molecule experiments at micromolar concentration are key for understanding the function of GQs and developing therapeutic applications.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Chemistry, Analytical
Tamaki Endoh, Jia-Heng Tan, Shuo-Bin Chen, Naoki Sugimoto
Summary: In this study, orthogonal light-up aptamers capable of simultaneously detecting multiple chemicals in living cells were developed, and a simple strategy for designing signaling aptamers was proposed, which will bring innovative advances in the field of RNA-based bioimaging.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Karthik Subramanian Chandrasekaran, Andrea Rentmeister
Review
Chemistry, Multidisciplinary
Katja Hartstock, Andrea Rentmeister
CHEMISTRY-A EUROPEAN JOURNAL
(2019)
Article
Biochemistry & Molecular Biology
Nils Muthmann, Theo Guez, Jean-Jacques Vasseur, Samie R. Jaffrey, Francoise Debart, Andrea Rentmeister
Article
Biochemistry & Molecular Biology
Jan Mauer, Miriam Sindelar, Vladimir Despic, Theo Guez, Ben R. Hawley, Jean-Jacques Vasseur, Andrea Rentmeister, Steven S. Gross, Livio Pellizzoni, Francoise Debart, Hani Goodarzis, Samie R. Jaffrey
NATURE CHEMICAL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Lea Anhaeuser, Sabine Huewel, Thomas Zobel, Andrea Rentmeister
NUCLEIC ACIDS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Kristina Rau, Lukas Roesner, Andrea Rentmeister
Article
Biochemical Research Methods
Lea Anhaeuser, Michael Teders, Andrea Rentmeister, Frank Glorius
Review
Cell Biology
Nils Muthmann, Katja Hartstock, Andrea Rentmeister
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2020)
Article
Chemistry, Multidisciplinary
Lea Anhaeuser, Nils Kloecker, Fabian Muttach, Florian Maesing, Petr Spacek, Armido Studer, Andrea Rentmeister
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2020)
Article
Biochemistry & Molecular Biology
Josef Leiter, Dennis Reichert, Andrea Rentmeister, Ronald Micura
Article
Multidisciplinary Sciences
Rainer Kurmayer, Elisabeth Entfellner, Thomas Weisse, Martin Offterdinger, Andrea Rentmeister, Li Deng
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Melissa van Duelmen, Andrea Rentmeister
Editorial Material
Chemistry, Multidisciplinary
Jennifer N. Andexer, Andrea Rentmeister
Article
Chemistry, Multidisciplinary
Kim J. Westerich, Karthik S. Chandrasekaran, Theresa Gross-Thebing, Nadine Kueck, Erez Raz, Andrea Rentmeister
Article
Chemistry, Multidisciplinary
Nicolas Cornelissen, Freideriki Michailidou, Fabian Muttach, Kristina Rau, Andrea Rentmeister
CHEMICAL COMMUNICATIONS
(2020)
