期刊
CHEMICAL COMMUNICATIONS
卷 50, 期 34, 页码 4478-4481出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc01549e
关键词
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资金
- DFG [RE 2796/2-1]
- Fonds der Chemischen Industrie
- Deutsche Forschungsgemeinschaft, DFG Cells in Motion - Cluster of Excellence, Munster, Germany [EXC 1003]
We present a chemo-enzymatic approach for site-specific labeling of 5'-capped RNAs based on bioorthogonal chemistry. A trimethylguanosine synthase was engineered to transfer a terminal azido moiety to the 5'-cap which could be further modified using strain-promoted azide-alkyne cycloaddition (SPAAC).
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