Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes
出版年份 2021 全文链接
标题
Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes
作者
关键词
-
出版物
Cells
Volume 10, Issue 7, Pages 1667
出版商
MDPI AG
发表日期
2021-07-05
DOI
10.3390/cells10071667
参考文献
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注意：仅列出部分参考文献，下载原文获取全部文献信息。- Metabolic engineering of HEK293 cells to improve transient transfection and cell budding of HIV‐1 virus‐like particles
- (2021) Jesús Lavado‐García et al. BIOTECHNOLOGY AND BIOENGINEERING
- Cell engineering for the production of hybrid-type N-glycans in HEK293 cells
- (2021) Ji-Xiong Leng et al. JOURNAL OF BIOCHEMISTRY
- Molecular characterization of HEK293 cells as emerging versatile cell factories
- (2021) Michela Pulix et al. CURRENT OPINION IN BIOTECHNOLOGY
- Multiplexed Quantitative Proteomic Analysis of HEK293 Provides Insights into Molecular Changes Associated with the Cell Density Effect, Transient Transfection, and Virus-Like Particle Production
- (2020) Jesús Lavado-García et al. JOURNAL OF PROTEOME RESEARCH
- Engineered transient and stable overexpression of translation factors eIF3i and eIF3c in CHOK1 and HEK293 cells gives enhanced cell growth associated with increased c-Myc expression and increased recombinant protein synthesis
- (2020) Anne Roobol et al. METABOLIC ENGINEERING
- Overexpression of ER Protein Processing and Apoptosis Regulator Genes in Human Embryonic Kidney 293 Cells Improves Gene Therapy Vectors Production
- (2020) Ana S. Formas‐Oliveira et al. Biotechnology Journal
- Cell Death in Culture: Molecular Mechanisms, Detections, and Inhibition Strategies
- (2020) Anandrao Ashok Patil et al. JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
- Knockout of the caspase 8‐associated protein 2 gene improves recombinant protein expression in HEK293 cells through up‐regulation of the cyclin‐dependent kinase inhibitor 2A gene
- (2020) Laura Abaandou et al. BIOTECHNOLOGY AND BIOENGINEERING
- Comprehensive characterization of dihydrofolate reductase‐mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies
- (2020) Sang Yoon Lee et al. Biotechnology Journal
- Homogeneously N-glycosylated proteins derived from the GlycoDelete HEK293 cell line enable diffraction-quality crystallogenesis
- (2020) Sandra Kozak et al. Acta Crystallographica Section D-Structural Biology
- Standardized, Scalable, and Timely Flexible Adeno-Associated Virus Vector Production Using Frozen High-Density HEK-293 Cell Stocks and CELLdiscs
- (2019) Benjamin Strobel et al. Human Gene Therapy Methods
- HEK293 Cells Overexpressing Nuclear Factor E2-Related Factor-2 Improve Expression of Recombinant Coagulation Factor VII
- (2019) Zahra Abbasi-Malati et al. MOLECULAR BIOTECHNOLOGY
- An anti-apoptotic HEK293 cell line provides a robust and high titer platform for transient protein expression in bioreactors
- (2019) Tia A Arena et al. mAbs
- Glycoengineering of HEK293 cells to produce high-mannose-type N-glycan structures
- (2019) Wei-Wei Ren et al. JOURNAL OF BIOCHEMISTRY
- Establishment of DHFR-deficient HEK293 cells for high yield of therapeutic glycoproteins
- (2019) Emmanuel Osei Mensah et al. JOURNAL OF BIOSCIENCE AND BIOENGINEERING
- A human expression system based on HEK293 for the stable production of recombinant erythropoietin
- (2019) Christine Lin Chin et al. Scientific Reports
- Improved protein expression in HEK293 cells by over-expressing miR-22 and knocking-out its target gene, HIPK1
- (2019) Sarah Inwood et al. New Biotechnology
- Glutamine synthetase gene knockout-human embryonic kidney 293E cells for stable production of monoclonal antibodies
- (2018) Da Young Yu et al. BIOTECHNOLOGY AND BIOENGINEERING
- Genetic disruption of multiple α1,2-mannosidases generates mammalian cells producing recombinant proteins with high-mannose–typeN-glycans
- (2018) Ze-Cheng Jin et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- Enhanced Production of Exosome-Associated AAV by Overexpression of the Tetraspanin CD9
- (2018) Lara Timantra Schiller et al. Molecular Therapy-Methods & Clinical Development
- Genome-wide CRISPR screens for Shiga toxins and ricin reveal Golgi proteins critical for glycosylation
- (2018) Songhai Tian et al. PLOS BIOLOGY
- Altering the central carbon metabolism of HEK293 cells: Impact on recombinant glycoprotein quality
- (2017) Eric Karengera et al. JOURNAL OF BIOTECHNOLOGY
- Transient expression of human antibodies in mammalian cells
- (2017) Rodrigo Vazquez-Lombardi et al. Nature Protocols
- Identifying HIPK1 as Target of miR-22-3p Enhancing Recombinant Protein Production From HEK 293 Cell by Using Microarray and HTP siRNA Screen
- (2017) Sarah Inwood et al. Biotechnology Journal
- Genome-scale RNA interference screen identifies antizyme 1 (OAZ1) as a target for improvement of recombinant protein production in mammalian cells
- (2016) Su Xiao et al. BIOTECHNOLOGY AND BIOENGINEERING
- Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFNα2 signal peptide
- (2016) Ramón Román et al. JOURNAL OF BIOTECHNOLOGY
- Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells
- (2016) Jared L. Spidel et al. JOURNAL OF IMMUNOLOGICAL METHODS
- Functional expression of human α7 nicotinic acetylcholine receptor in human embryonic kidney 293 cells
- (2016) Yuan Gong et al. Molecular Medicine Reports
- Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector From the Culture Media for GMP FIX and FLT1 Clinical Vector
- (2016) Joshua C Grieger et al. MOLECULAR THERAPY
- Development and Optimization of AAV hFIX Particles by Transient Transfection in an iCELLis®Fixed-Bed Bioreactor
- (2016) Alicia D. Powers et al. Human Gene Therapy Methods
- A Guide to Transient Expression of Membrane Proteins in HEK-293 Cells for Functional Characterization
- (2016) Amanda Ooi et al. Frontiers in Physiology
- Distribution of AAV8 particles in cell lysates and culture media changes with time and is dependent on the recombinant vector
- (2016) Bryan A Piras et al. Molecular Therapy-Methods & Clinical Development
- Selection and optimization of transfection enhancer additives for increased virus-like particle production in HEK293 suspension cell cultures
- (2015) Laura Cervera et al. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
- Manufacturing process used to produce long-acting recombinant factor VIII Fc fusion protein
- (2015) Justin McCue et al. BIOLOGICALS
- Increased titer and reduced lactate accumulation in recombinant retrovirus production through the down-regulation of HIF1 and PDK
- (2015) A. F. Rodrigues et al. BIOTECHNOLOGY AND BIOENGINEERING
- MiRNA mimic screen for improved expression of functional neurotensin receptor from HEK 293 cells
- (2015) Su Xiao et al. BIOTECHNOLOGY AND BIOENGINEERING
- Rational plasmid design and bioprocess optimization to enhance recombinant adeno-associated virus (AAV) productivity in mammalian cells
- (2015) Verena V. Emmerling et al. Biotechnology Journal
- Differences in N-glycosylation of recombinant human coagulation factor VII derived from BHK, CHO, and HEK293 cells
- (2015) Ernst Böhm et al. BMC BIOTECHNOLOGY
- Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems
- (2015) Christine M Thompson et al. BMC BIOTECHNOLOGY
- Human cell lines for biopharmaceutical manufacturing: history, status, and future perspectives
- (2015) Jennifer Dumont et al. CRITICAL REVIEWS IN BIOTECHNOLOGY
- HEK293 in cell biology and cancer research: phenotype, karyotype, tumorigenicity, and stress-induced genome-phenotype evolution
- (2015) A.A. Stepanenko et al. GENE
- Human cell lines for the production of recombinant proteins: on the horizon
- (2015) Lukas Fliedl et al. New Biotechnology
- Rational plasmid design and bioprocess optimization to enhance recombinant adeno-associated virus (AAV) productivity in mammalian cells
- (2015) Verena V. Emmerling et al. Biotechnology Journal
- Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature
- (2015) Chi-Yen Lin et al. PLoS One
- Validation of the manufacturing process used to produce long-acting recombinant factor IX Fc fusion protein
- (2014) J. McCue et al. HAEMOPHILIA
- GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins
- (2014) Leander Meuris et al. NATURE BIOTECHNOLOGY
- Simply better glycoproteins
- (2014) Bernd Lepenies et al. NATURE BIOTECHNOLOGY
- Over-expression of secreted proteins from mammalian cell lines
- (2014) Annamarie C. Dalton et al. PROTEIN SCIENCE
- Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations
- (2014) Yao-Cheng Lin et al. Nature Communications
- A simplified purification protocol for recombinant adeno-associated virus vectors
- (2014) Mark Potter et al. Molecular Therapy-Methods & Clinical Development
- High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells
- (2013) Volker Jäger et al. BMC BIOTECHNOLOGY
- Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology
- (2013) A Radu Aricescu et al. CURRENT OPINION IN STRUCTURAL BIOLOGY
- Exploiting the metabolism of PYC expressing HEK293 cells in fed-batch cultures
- (2013) Cédric Vallée et al. JOURNAL OF BIOTECHNOLOGY
- Production of adeno-associated virus (AAV) serotypes by transient transfection of HEK293 cell suspension cultures for gene delivery
- (2013) Parminder Singh Chahal et al. JOURNAL OF VIROLOGICAL METHODS
- Metabolic pathways recruited in the production of a recombinant enveloped virus: Mining targets for process and cell engineering
- (2013) A.F. Rodrigues et al. METABOLIC ENGINEERING
- Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
- (2013) Ophir Shalem et al. SCIENCE
- Expression of Recombinant Antibodies
- (2013) André Frenzel et al. Frontiers in Immunology
- The first recombinant human coagulation factor VIII of human origin: human cell line and manufacturing characteristics
- (2012) Elisabeth Casademunt et al. EUROPEAN JOURNAL OF HAEMATOLOGY
- Biochemical and functional characterization of a recombinant monomeric factor VIII-Fc fusion protein
- (2012) R. T. PETERS et al. JOURNAL OF THROMBOSIS AND HAEMOSTASIS
- Overexpression of membrane proteins in mammalian cells for structural studies
- (2012) Juni Andréll et al. MOLECULAR MEMBRANE BIOLOGY
- A Multi-Omics Analysis of Recombinant Protein Production in Hek293 Cells
- (2012) Stefanie Dietmair et al. PLoS One
- Human cells: New platform for recombinant therapeutic protein production
- (2012) Kamilla Swiech et al. PROTEIN EXPRESSION AND PURIFICATION
- Characterisation of the post-translational modifications of a novel, human cell line-derived recombinant human factor VIII
- (2012) Christoph Kannicht et al. THROMBOSIS RESEARCH
- Enhanced glycoprotein production in HEK-293 cells expressing pyruvate carboxylase
- (2011) Olivier Henry et al. METABOLIC ENGINEERING
- Efficient expression screening of human membrane proteins in transiently transfected Human Embryonic Kidney 293S cells
- (2011) Sarika Chaudhary et al. METHODS
- Identification of cellular genes critical to recombinant protein production using a Gaussia luciferase-based siRNA screening system
- (2010) Teng Rhui Lwa et al. JOURNAL OF BIOTECHNOLOGY
- Production of therapeutic antibodies with controlled fucosylation
- (2010) Naoko Yamane-Ohnuki et al. mAbs
- A functional analysis of N-glycosylation-related genes on sialylation of recombinant erythropoietin in six commonly used mammalian cell lines
- (2010) Peiqing Zhang et al. METABOLIC ENGINEERING
- Implications of the presence of N-glycolylneuraminic acid in recombinant therapeutic glycoproteins
- (2010) Darius Ghaderi et al. NATURE BIOTECHNOLOGY
- Scalable production of influenza virus in HEK-293 cells for efficient vaccine manufacturing
- (2010) Audrey Le Ru et al. VACCINE
- The effect of Bcl-2, YAMA, and XIAP over-expression on apoptosis and adenovirus production in HEK293 cell line
- (2009) Kalbinder Singh Sandhu et al. BIOTECHNOLOGY AND BIOENGINEERING
- Enhancement of recombinant antibody production in HEK 293E cells by WPRE
- (2009) Keun-Soo Kim et al. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- High AAV vector purity results in serotype- and tissue-independent enhancement of transduction efficiency
- (2009) E Ayuso et al. GENE THERAPY
- Development of a scalable process for high-yield lentiviral vector production by transient transfection of HEK293 suspension cultures
- (2009) Sven Ansorge et al. JOURNAL OF GENE MEDICINE
- The vesicle-trafficking protein munc18b increases the secretory capacity of mammalian cells
- (2009) Ren-Wang Peng et al. METABOLIC ENGINEERING
- Molecular engineering of exocytic vesicle traffic enhances the productivity of Chinese hamster ovary cells
- (2008) Ren-Wang Peng et al. BIOTECHNOLOGY AND BIOENGINEERING
- Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells
- (2008) Martin Loignon et al. BMC BIOTECHNOLOGY
- An XBP-1 dependent bottle-neck in production of IgG subtype antibodies in chemically defined serum-free Chinese hamster ovary (CHO) fed-batch processes
- (2008) E BECKER et al. JOURNAL OF BIOTECHNOLOGY
- Membrane fusion
- (2008) William Wickner et al. NATURE STRUCTURAL & MOLECULAR BIOLOGY
- Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
- (2008) Gaurav Backliwal et al. NUCLEIC ACIDS RESEARCH
- Enhanced functional recombinant factor VII production by HEK 293 cells stably transfected with VKORC1 where the gamma-carboxylase inhibitor calumenin is stably suppressed by shRNA transfection
- (2008) Nadeem Wajih et al. THROMBOSIS RESEARCH
- The Recognition and Retrotranslocation of Misfolded Proteins from the Endoplasmic Reticulum
- (2008) Kunio Nakatsukasa et al. TRAFFIC
- Serum-free transient protein production system based on adenoviral vector and PER.C6 technology: High yield and preserved bioactivity
- (2007) M.J.E. Havenga et al. BIOTECHNOLOGY AND BIOENGINEERING
- Effect of Increased Expression of Protein Disulfide Isomerase and Heavy Chain Binding Protein on Antibody Secretion in a Recombinant CHO Cell Line
- (2005) Nicole Borth et al. BIOTECHNOLOGY PROGRESS
- Identification and Use of Zinc Finger Transcription Factors That Increase Production of Recombinant Proteins in Yeast and Mammalian Cells
- (2005) Kyung-Soon Park et al. BIOTECHNOLOGY PROGRESS
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