Review
Biotechnology & Applied Microbiology
Hui-Ning Liu, Wei-Hua Dong, Yan Lin, Zhao-Hui Zhang, Tian-Yun Wang
Summary: This review discusses the effect and mechanism of miRNAs on the expression level and quality of recombinant proteins in CHO cells, indicating that miRNAs can enhance protein expression in CHO cells while also affecting the quality of RTPs.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mengzhen Yue, Bing Hu, Jiajia Li, Ruifeng Chen, Zhen Yuan, Hurong Xiao, Haishuang Chang, Yaming Jiu, Kun Cai, Binbin Ding
Summary: The coronavirus ORF6 protein targets lipid droplets, localizes to the mitochondria-LD and ER-LD contact sites, and regulates LD biogenesis and lipolysis. It interacts with ER membrane proteins BAP31 and USE1 to mediate ER-LDs contact formation and links the SAM complex in the mitochondrial outer membrane to LDs. ORF6 promotes cellular lipolysis and LD biogenesis to reprogram host cell lipid flux and facilitate viral production.
Article
Cell Biology
Yi Sheng, Guang Yang, Zachary Markovich, Sung Min Han, Rui Xiao
Summary: Proteotoxic stress is a common challenge for all organisms, and the evolutionarily conserved unfolded protein responses (UPRs) play a key role in defending such stress. Different subcellular compartments show distinct temporal regulation of UPRs during aging, with UPRs elevating with age but their inducibility declining. Different types of UPRs have varied temporal requirements for induction, and different tissues may exhibit distinct temporal profiles of UPR inducibility during aging.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Endocrinology & Metabolism
Imke L. Lemmer, Nienke Willemsen, Nazia Hilal, Alexander Bartelt
Summary: This review provides a general guide to understanding the nature, etiology, and consequences of ER stress in metabolic disorders, emphasizing key mechanisms such as protein quality control, lipid and carbohydrate metabolism, and inflammation.
MOLECULAR METABOLISM
(2021)
Review
Medicine, Research & Experimental
Shengkai Xia, Wenzhe Duan, Wenwen Liu, Xinri Zhang, Qi Wang
Summary: GRP78 is a key protein in the pathological processes of lung cancer, involved in protein folding, cellular stress response to microenvironment stimuli, and closely associated with the progression and poor prognosis of lung cancer.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Chang Ho Kang, Eun Seon Lee, Ganesh M. Nawkar, Joung Hun Park, Seong Dong Wi, Su Bin Bae, Ho Byoung Chae, Seol Ki Paeng, Jong Chan Hong, Sang Yeol Lee
Summary: The key regulator of light signaling, COP1, plays a crucial role in plant endoplasmic reticulum stress response by degrading HY5 in the nucleus, facilitating the unfolded protein response activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Xiaoyu Wang, Fan Yang, Xiaomin Tian, Haihua Huo, Xinrun Li, Haitong Wu, Jianying Guo
Summary: Excessive copper can cause histopathological damage to duck cerebrums, disrupt the balance of trace elements, induce oxidative stress and activation of ER quality control, resulting in duck cerebrums damage.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Multidisciplinary Sciences
Feiran Li, Yu Chen, Qi Qi, Yanyan Wang, Le Yuan, Mingtao Huang, Ibrahim E. Elsemman, Amir Feizi, Eduard J. Kerkhoven, Jens Nielsen
Summary: Researchers have constructed a proteome-constrained genome-scale protein secretory model for yeast and demonstrated its application in the production of different recombinant proteins.
NATURE COMMUNICATIONS
(2022)
Review
Biotechnology & Applied Microbiology
Hana Raschmanova, Astrid Weninger, Zdenek Knejzlik, Karel Melzoch, Karin Kovar
Summary: Protein folding and processing in the endoplasmic reticulum can hinder protein production and secretion in Pichia pastoris. The unfolded protein response pathway is activated in response to misfolded proteins, upregulating chaperones and folding-assisting proteins. Co-expression of specific UPR genes with the recombinant gene of interest is a common strategy to enhance protein production, but can lead to decreased productivity if not balanced properly.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Editorial Material
Cell Biology
Hana Popelka, Daniel J. J. Klionsky
Summary: A recent study used cryo-electron tomography combined with computational analysis to gain insights into autophagosome biogenesis in yeast cells. This approach provided new information on autophagic structures, their contacts with organelles, membrane sources, and transition mechanisms. These findings open new avenues for autophagy research and highlight the potential of cryo-ET in cell biology.
Review
Cell Biology
Sara Benhammouda, Anjali Vishwakarma, Priya Gatti, Marc Germain
Summary: Organelles in cells cooperate through membrane contact sites to regulate vital cellular functions, with Mitochondria-Endoplasmic-Reticulum (ER) contact sites (MERCS) playing a crucial role in controlling biological processes by regulating calcium and metabolic homeostasis. Various techniques, including fluorescence-based imaging, have been established to study these contact sites and identify molecular interactions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Plant Sciences
Sofia Reyes-Impellizzeri, Adrian A. Moreno
Summary: This review focuses on the impact of alterations in components of ERQC, ERAD, and UPR on plant responses to abiotic stresses. Evidence shows a clear connection between ERAD and UPR mechanisms, but the connection of ERQC components with these processes or their possible client proteins is lacking. Proteomics approaches are suggested to uncover the identity of these proteins and their connection to ER proteostasis.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Cell Biology
Ming Yang, Shilu Luo, Xi Wang, Chenrui Li, Jinfei Yang, Xuejing Zhu, Li Xiao, Lin Sun
Summary: The endoplasmic reticulum (ER) is crucial for maintaining cellular homeostasis, and ER-phagy, a selective form of autophagy, helps remove damaged ER to protect cells from harm caused by excessive ER stress. Many receptor-mediated ER-phagy pathways have been discovered in recent years, highlighting the importance of this newly identified autophagy process in maintaining cellular health.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gehan Botrus, Richard M. Miller, Pedro Luiz Serrano Uson Jr, Geoffrey Kannan, Haiyong Han, Daniel D. Von Hoff
Summary: High levels of ER stress and UPR activation are present in pancreatic cancer, leading to adaptive mechanisms and potential apoptosis. This review discusses the mechanisms by which compounds activate the UPR pathways and induce apoptosis, and explores the potential of ER stress inducers for anti-tumor efficacy in pancreatic cancer. A new approach of increasing ER stress and UPR activation to incite apoptotic cell death in pancreatic cancer is hypothesized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Valerie Perea, Christian Cole, Justine Lebeau, Vivian Dolina, Kelsey R. Baron, Aparajita Madhavan, Jeffery W. Kelly, Danielle A. Grotjahn, R. Luke Wiseman
Summary: Endoplasmic reticulum (ER) stress and mitochondrial dysfunction are interconnected in the development of various diseases. The PERK signaling pathway, an arm of the unfolded protein response (UPR), plays a significant role in regulating mitochondrial biology during ER stress. This study reveals that PERK activity promotes adaptive remodeling of mitochondrial membrane phosphatidic acid (PA), leading to protective mitochondrial elongation in response to acute ER stress. These findings highlight a novel role for PERK in modulating mitochondrial phospholipids and demonstrate its involvement in adapting organellar shape during ER stress.