期刊
VACCINE
卷 28, 期 21, 页码 3661-3671出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2010.03.029
关键词
Influenza virus; HEK-293; Suspension culture; Serum-free medium; Bioreactor; Vaccine production
资金
- ACT group at BRI
Cell culture processes offer an attractive alternative to conventional chicken egg-based influenza vaccine production methods. However, most protocols still rely on the use of adherent cells, which makes process scale-up a challenging issue. In this study, it is demonstrated that the HEK-293 human cell line is able to efficiently replicate influenza virus. Production in serum-free suspension of HEK-293 cultures resulted in high titers of infectious influenza viruses for different subtypes and variants including A/H1, A/H3 and B strains. After virus adaptation and optimization of infection conditions, production in 3-L bioreactor resulted in titers of up to 10(9) IVP/mL demonstrating the scale-up potential of the process. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.
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