Review
Biochemistry & Molecular Biology
Irina P. Voronova
Summary: This review discusses the influence of serotonin receptors on body temperature regulation in warm-blooded animals, covering different receptor types and their activation effects on temperature.
Review
Cell Biology
Qiangqiang He, Meiyu Qu, Tingyu Shen, Jiakun Su, Yana Xu, Chengyun Xu, Muhammad Qasim Barkat, Jibao Cai, Haibin Zhu, Ling -Hui Zeng, Ximei Wu
Summary: Mitochondria-associated endoplasmic reticulum membranes (MAMs) are dynamic coupling structures between mitochondria and the endoplasmic reticulum (ER), playing important roles in calcium homeostasis, autophagy, ER stress, lipid metabolism, and various diseases. The formation and function of MAMs rely on specific proteins, including the IP3R-Grp75-VDAC complex. S-palmitoylation, a reversible protein post-translational modification, is closely related to the localization of proteins in MAMs. This article provides an overview of the composition and function of MAMs, focusing on the role of S-palmitoylated proteins in calcium flux, lipid rafts, and discusses their implication in diseases, particularly neurodegenerative diseases (NDs). Potential drug compounds targeting S-palmitoylation are also proposed.
AGEING RESEARCH REVIEWS
(2023)
Article
Neurosciences
Natsumi Ageta-Ishihara, Sayaka Takemoto-Kimura, Yayoi Kondo, Michiko Okamura, Haruhiko Bito
Summary: CLICK-III/CaMKI? is a lipid-anchored neuronal isoform of Ca2+/calmodulin-dependent protein kinases that mediates BDNF-dependent dendritogenesis. Prenylation and palmitoylation of CaMKI? control its association with detergent-resistant microdomains and are essential for its dendritogenic activity. Prenylation permits membrane anchoring and Golgi access, while palmitoylation facilitates association with cholesterol-enriched lipid microdomains.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Barbara A. Bricker, Chandrashekhar Voshavar, Edem K. Onyameh, Uma M. Gonela, Xinsong Lin, Tracy L. Swanson, Laura B. Kozell, Jennifer L. Schmachtenberg, Shelley H. Bloom, Aaron J. Janowsky, Seth Y. Ablordeppey
Summary: In this study, we synthesized and separated the enantiomers of SYA0340, identified their absolute configurations, and evaluated their binding affinities and functional characteristics at the 5-HT1A and 5-HT7A receptors. The results showed that both enantiomers have similar agonist properties at the 5-HT1A receptor and antagonist properties at the 5-HT7A receptor, with SYA0340-P1 displaying higher potency as a 5-HT1A receptor antagonist. Therefore, SYA0340-P1 is considered the eutomer and these enantiomers may serve as new pharmacological probes for the 5-HT1A and 5-HT7A receptors.
Article
Biochemistry & Molecular Biology
Martyna Z. Wrobel, Andrzej Chodkowski, Maciej Dawidowski, Agata Siwek, Katarzyna Stachowicz, Bernadeta Szewczyk, Gabriel Nowak, Grzegorz Satala, Andrzej J. Bojarski, Jadwiga Turlo
Summary: The synthesis and evaluation of new compounds targeting the serotonin 1A receptors and serotonin transporter in the treatment of central nervous system diseases, especially depression, were conducted. Compound 4f emerged as the most promising with good activity, stability, and potential as a therapeutic agent.
BIOORGANIC CHEMISTRY
(2023)
Article
Clinical Neurology
Darakhshan Jabeen Haleem
Summary: The global prevalence of depression is increasing, but the current treatment outcome is unsatisfactory. Stress-related epigenetic changes and the role of serotonin and 5-HT1A receptors in the pathogenesis and treatment of depression are important. Synthetic compounds targeting GC and 5-HT1A receptors may be better therapeutic agents for depression.
Review
Biochemistry & Molecular Biology
Dasiel O. Borroto-Escuela, Patrizia Ambrogini, Barbara Chruscicka, Maria Lindskog, Minerva Crespo-Ramirez, Juan C. Hernandez-Mondragon, Miguel Perez de la Mora, Harriet Schellekens, Kjell Fuxe
Summary: Communication through serotonin mainly occurs in the extracellular space and cerebrospinal fluid, with disturbances in receptor-receptor interactions potentially contributing to major depression. Oxytocin receptors play a significant role in modulating social and cognitive behaviors, and their dysfunction may lead to psychiatric diseases like depression. Targeting 5-HTR heterocomplexes could be a novel approach for treating major depression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Carla Arganaraz, Tamara S. Adjimann, Paula P. Perissinotti, Mariano Soiza-Reilly
Summary: Serotonin neurons are involved in anxiety and depression. This study investigated the developmental trajectory of synaptic inputs to these neurons and found that cortical glutamate and subcortical GABA inputs undergo refinement during postnatal development, while subcortical glutamate inputs do not. This refinement process is accompanied by the presence of inhibitory mechanisms. These findings contribute to our understanding of neurodevelopmental vulnerability to psychiatric disorders.
Article
Physiology
Sara R. Roig, Silvia Cassinelli, Andre Zeug, Evgeni Ponimaskin, Antonio Felipe
Summary: Members of the regulatory Kv beta family play a role in modulating the kinetics and traffic of voltage-dependent Kv channels. The formation of heteromeric complexes between Kv beta subtypes influences the spatial distribution in lipid rafts and can alter the immune response. Differentially regulating Kv beta subtypes can modulate Kv beta-dependent physiological responses and the architecture of Kv beta heterotetramers.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Clinical Neurology
Rafael Pazinatto de Aguiar, Adrian Newman-Tancredi, Jos Prickaerts, Rubia Maria Weffort de Oliveira
Summary: This review focuses on the roles and mechanisms of 5-HT1A receptors in neuroprotection in experimental models of cerebral ischemia, with experimental evidence suggesting that 5-HT1A receptor agonists can prevent neuronal damage and promote functional recovery induced by ischemia.
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
(2021)
Review
Pharmacology & Pharmacy
Adrian Newman-Tancredi, Ronan Y. Depoortere, Mark S. Kleven, Marcin Kolaczkowski, Luc Zimmer
Summary: Biased agonism at G-protein-coupled receptors has gained increasing interest for the discovery of more effective and safer drugs. However, most studies are limited to in vitro tests, and few biased agonists have been tested in vivo. The study of biased agonism at 5-HT1A receptors has been limited by the availability of ligands with poor target selectivity or partial agonism. However, the development of new selective and efficacious agonists has advanced the study of biased agonism at this receptor and opened up new therapeutic opportunities.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Pharmacology & Pharmacy
William H. Powell, Lucy E. Annett, Ronan Depoortere, Adrian Newman-Tancredi, Mahmoud M. Iravani
Summary: Anxiety is a common neuropsychiatric disorder with abnormalities in serotonergic function playing a role in its pathogenesis. NLX-112, a highly selective 5-HT1A receptor full agonist, has been shown to alleviate anxiety-like behaviors in mice, potentially possessing anxiolytic properties.
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2022)
Article
Neurosciences
Arianna Gentile Polese, Sunny Nigam, Laura M. Hurley
Summary: The study found that serotonin in the inferior colliculus may have differential effects on auditory processing of different BBVs, partly through inhibition of the 5-HT1A receptor.
FRONTIERS IN NEURAL CIRCUITS
(2021)
Article
Neurosciences
Marcus Saarinen, Ioannis Mantas, Ivana Flais, Richard Agren, Kristoffer Sahlholm, Mark J. Millan, Per Svenningsson
Summary: SEP-383856 (SEP-856) is a novel antipsychotic with a unique pattern of receptor interaction, showing agonist activity at TAAR1 and 5-HT1A receptors. Research indicates its potential advantages in treating schizophrenia and other psychotic disorders.
NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Hirohito Esaki, Yuki Sasaki, Naoya Nishitani, Hikari Kamada, Satoko Mukai, Yoshitaka Ohshima, Sao Nakada, Xiyan Ni, Satoshi Deyama, Katsuyuki Kaneda
Summary: This study examined whether the prosocial effects induced by MDMA are mediated by 5-HT neurotransmission in the medial prefrontal cortex (mPFC) and the basolateral nucleus of amygdala (BLA). The results showed that selective inhibition of 5-HT transporters before MDMA administration did not suppress the prosocial effects. However, the 5-HT1A receptor antagonist WAY100635 significantly suppressed the prosocial effects of MDMA. Furthermore, local administration of WAY100635 into the BLA, but not the mPFC, suppressed the MDMA-induced prosocial effects. These findings suggest that MDMA induces prosocial effects through the stimulation of 5-HT1A receptors in the BLA.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Review
Cell Biology
Izabela Figiel, Patrycja K. Kruk, Monika Zareba-Koziol, Paulina Rybak, Monika Bijata, Jakub Wlodarczyk, Joanna Dzwonek
Summary: The extracellular matrix plays a critical role in synaptic function, with MMP-9 and Rho GTPases identified as key factors in regulating synaptic plasticity. MMP-9 contributes to dynamic remodeling by cleaving ECM components, while Rho GTPases control cellular processes in brain physiology and pathology.
Article
Cell Biology
Simon Bennet Sonnenberg, Jonah Rauer, Christoph Gohr, Nataliya Gorinski, Sophie Kristin Schade, Dalia Abdel Galil, Vladimir Naumenko, Andre Zeug, Stephan C. Bischoff, Evgeni Ponimaskin, Daria Guseva
Summary: The study uncovered a signaling pathway involving L1CAM and 5-HT4 receptor that facilitates dendritic spine maturation and affects memory formation.
JOURNAL OF CELL SCIENCE
(2021)
Review
Geriatrics & Gerontology
Ewa Sikora, Anna Bielak-Zmijewska, Magdalena Dudkowska, Adam Krzystyniak, Grazyna Mosieniak, Malgorzata Wesierska, Jakub Wlodarczyk
Summary: Aging of the brain can result in memory and cognitive decline, often coinciding with changes in the structural plasticity of dendritic spines. Decreased number and maturity of spines in aged individuals, along with alterations in synaptic transmission, may reflect impaired brain functions. The involvement of cellular senescence in brain aging is being increasingly explored, with evidence suggesting a potential role in the aging process similar to other organs.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Ewa Baczynska, Katarzyna Karolina Pels, Subhadip Basu, Jakub Wlodarczyk, Blazej Ruszczycki
Summary: This article reviews experimental approaches designed to assess quantitative features of dendritic spines under physiological stimuli and in pathological conditions. By comparing various methodological pipelines and systematically summarizing the methodology and results of relevant experiments, the focus is on quantitative data regarding the number of animals, cells, dendritic spines, types of studied parameters, size of observed changes, and their statistical significance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Neurosciences
Nicole Kerkenberg, Lydia Wachsmuth, Mingyue Zhang, Christiane Schettler, Evgeni Ponimaskin, Cornelius Faber, Bernhard T. Baune, Weiqi Zhang, Christa Hohoff
Summary: Recent research suggests that developmental brain changes in mice can last up to 6 months, with adolescence coinciding with significant alterations in neuronal structure and function affecting connectivity between brain regions. The study demonstrates that ZDHHC7 plays a role in modulating hippocampal and mPFC microstructure development, as deficiency of this enzyme impairs fiber development and leads to increased hippocampal volume.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Article
Neurosciences
Franziska E. Mueller, Volodymyr Cherkas, Gebhard Stopper, Laura C. Caudal, Laura Stopper, Frank Kirchhoff, Christian Henneberger, Evgeni G. Ponimaskin, Andre Zeug
Summary: Recent research has introduced a biophysically-based analytical concept, the pixel-based multi-threshold event detection (MTED), to comprehensively analyze the complex spatio-temporal changes of Ca2+ biosensor fluorescence in astrocytes. The MTED strategy, as a parameter-free approach, is easily transferable to other fluorescent indicators and biosensors, promoting standardized procedures and parameters to enhance research data comparability.
Article
Biochemistry & Molecular Biology
Soumyendu Sekhar Bandyopadhyay, Anup Kumar Halder, Monika Zareba-Koziol, Anna Bartkowiak-Kaczmarek, Aviinandaan Dutta, Piyali Chatterjee, Mita Nasipuri, Tomasz Wojtowicz, Jakub Wlodarczyk, Subhadip Basu
Summary: The study introduces a consensus strategy for predicting palmitoylated cysteine sites on synaptic proteins, which significantly improves prediction performance in mouse datasets through efficient feature selection and a three-star quality consensus strategy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Monika Zareba-Koziol, Anna Bartkowiak-Kaczmarek, Matylda Roszkowska, Krystian Bijata, Izabela Figiel, Anup Kumar Halder, Paulina Kaminska, Franziska E. Mueller, Subhadip Basu, Weiqi Zhang, Evgeni Ponimaskin, Jakub Wlodarczyk
Summary: Sex differences in the brain are associated with differences in neuronal morphology, synaptic plasticity, and molecular signaling pathways. S-PALM mechanism plays a crucial role in regulating synaptic integrity and neuronal signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Computer Science, Interdisciplinary Applications
Nirmal Das, Ewa Baczynska, Monika Bijata, Blazej Ruszczycki, Andre Zeug, Dariusz Plewczynski, Punam Kumar Saha, Evgeni Ponimaskin, Jakub Wlodarczyk, Subhadip Basu
Summary: Segmentation and analysis of dendritic spine morphology face challenges in individual spine segmentation and quantitative assessment of spine morphology. We developed 3dSpAn software based on the 3D multi-scale opening algorithm, with four modules for preprocessing, segmentation, and feature extraction. Results show usability across different observation methods, reproducibility, and accuracy, with the software freely available for non-commercial use.
Article
Multidisciplinary Sciences
Weilun Sun, Ilseob Choi, Stoyan Stoyanov, Oleg Senkov, Evgeni Ponimaskin, York Winter, Janelle M. P. Pakan, Alexander Dityatev
Summary: The study demonstrates that neurons in the retrosplenial cortex are capable of encoding multiple task-related dimensions across learning, particularly showing enhanced performance in tasks requiring cognitive flexibility. Chemogenetic inactivation of the RSC disrupts behavioral context discrimination during learning phases, but does not affect recall of previously formed associations, suggesting a crucial role for the RSC in context-value updating.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Izabela Figiel, Ewa Baczynska, Tomasz Wojtowicz, Marta Magnowska, Anna Buszka, Monika Bijata, Jakub Wlodarczyk
Summary: This study reveals that the deletion of DG in rat hippocampal cell cultures leads to significant changes in the density and morphology of dendritic spines, accompanied by a decrease in laminin and aquaporin 4. However, silencing of DG does not affect synaptic transmission or Psd-95 protein expression, suggesting the existence of unknown mechanisms to maintain proper synaptic signaling despite impaired structure of dendritic spines. Presumably, astrocytes are involved in these processes.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Tatiana Ilchibaeva, Anton Tsybko, Andre Zeug, Franziska E. Mueller, Daria Guseva, Stephan Bischoff, Evgeni Ponimaskin, Vladimir Naumenko
Summary: The study demonstrates a physical interaction between the serotonin receptor 5-HT2A and the tropomyosin receptor kinase B (TrkB) and shows their regulated expression in different brain regions. These findings provide insights into the molecular mechanisms underlying the modulation of TrkB functions during brain development and in pathological conditions.
Article
Cell Biology
Catia Domingos, Franziska E. Mueller, Stefan Passlick, Dagmar Wachten, Evgeni Ponimaskin, Martin K. Schwarz, Susanne Schoch, Andre Zeug, Christian Henneberger
Summary: The increase in RhoA activity leads to changes in astrocyte morphology, particularly the withdrawal of perisynaptic processes. These changes were replicated in vitro and in vivo, and overexpression of RhoA-CA specifically resulted in a significant reduction in fine peripheral astrocytic processes in vivo.
Article
Cell Biology
Adam Krzystyniak, Malgorzata Wesierska, Gregory Petrazzo, Agnieszka Gadecka, Magdalena Dudkowska, Anna Bielak-Zmijewska, Grazyna Mosieniak, Izabela Figiel, Jakub Wlodarczyk, Ewa Sikora
Summary: By conducting experiments on aged rats, it was found that the use of senolytic agents D+Q can improve the learning and memory abilities of aged rats, reduce peripheral inflammation, and regulate the structure of hippocampal neurons and histone modifications. Moreover, the benefits of this treatment are long-lasting, even after the cessation of drug administration.
