Article
Cell Biology
Lee Armstrong, Colin E. Willoughby, Declan J. Mckenna
Summary: This study investigated the association between miR-143-3p and EMT in prostate cancer. The findings showed that miR-143-3p expression was significantly decreased in prostate cancer cells and it was associated with the EMT-associated gene AKT1. Further analysis suggested that miR-143-3p may serve as a predictor of disease recurrence in prostate cancer, making it a potential diagnostic and prognostic biomarker.
Article
Oncology
Jia Xu, Xi Li, Purong Zhang, Jie Luo, Exian Mou, Shiwei Liu
Summary: miR-143-5p exhibits low expression in breast cancer tissues and suppresses breast cancer cell proliferation, colony formation, and migration. The antitumor effects of miR-143-5p are mediated through the regulation of the HIF-1 alpha-related GLUT1 pathway.
Article
Biotechnology & Applied Microbiology
Yunchi Zhu, Xin Liao, Tingyu Han, J. -y. Chen, Chunpeng He, Zuhong Lu
Summary: This study investigates the gene regulation in coral-Symbiodiniaceae holobionts by integrating transcriptomes and small RNAs of corals and Cladocopium genomes. The discovery of novel miRNAs and their role in regulating rejection from host coral cells and protecting symbiont from autophagy and apoptosis provide evidence for the early origin of cross-kingdom regulation and contribute to our understanding of coral-Symbiodiniaceae holobionts.
Article
Biochemistry & Molecular Biology
Lixia Zhang, Jinning Gao, Anjing Gong, Yanhan Dong, Xiaodan Hao, Xuekang Wang, Jian Zheng, Wenmeng Ma, Yiying Song, Jie Zhang, Wenhua Xu
Summary: The study explored the role of LINC00963 in regulating corneal fibrosis, finding that its overexpression can inhibit the formation of myofibroblasts and affect the contractility and secretion of collagen I and III in corneal fibroblasts by downregulating miR-143-3p expression. The research suggests LINC00963 as a promising therapeutic target for corneal fibrosis.
DNA AND CELL BIOLOGY
(2022)
Review
Pathology
Vahid Asghariazar, Mahtab Kadkhodayi, Mehdi Sarailoo, Amir Ghaffari Jolfayi, Behzad Baradaran
Summary: This review outlines the impact of miR-143 expression levels on cancer progression, discussing its roles as a tumor suppressor or an oncogene, and explores its target genes and relevant signaling pathways.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Chemistry, Multidisciplinary
Qingtao Meng, Hao Sun, Shenshen Wu, Giuseppe Familiari, Michela Relucenti, Michael Aschner, Xiaobo Li, Rui Chen
Summary: This study reveals that a viral microRNA called EBV-miR-BART18-3p is highly expressed in colorectal cancer (CRC) and is closely associated with the pathological and clinical stages of CRC. The results show that EBV-miR-BART18-3p promotes CRC development by activating the lipogenesis pathway, and it may serve as a potential clinical intervention target for CRC.
Review
Food Science & Technology
Fatemeh Sadoughi, Parisa Maleki Dana, Zatollah Asemi, Bahman Yousefi
Summary: Curcumin, as a beneficial plant compound, has the ability to modulate miRNA expression and have impacts on cancer hallmarks. MiRNAs are capable of regulating gene expression and signaling, as well as modulating cell differentiation and cell cycle, making them great candidates for personalized cancer treatment.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Cell Biology
Manal A. A. Moustafa, Durbba Nath, John J. Georrge, Supriyo Chakraborty
Summary: This study investigated the overexpressed genes and their associated microRNAs in oral cancer. Using in-house software, specific microRNAs targeting these genes were identified and their interactions were evaluated. The stability of target regions and the GC content of the overexpressed genes were analyzed. Gene ontology analysis was performed to understand the involvement of these genes in biological processes, molecular functions, and cellular components.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Jing Li, Xianmei Zhong, Yueshui Zhao, Jing Shen, Chalermchai Pilapong, Zhangang Xiao
Summary: Lung cancer is the second leading cause of cancer-related death globally. Abnormal expression of microRNAs has been found in lung cancer and they play important roles in its initiation and progression. Polyphenols, natural bioactive chemicals synthesized by plants, have shown promising anticancer effects against lung cancer and other types of cancer. Recent studies have revealed that polyphenols exert their anticancer effects by regulating the expression levels of microRNAs in lung cancer. Therefore, targeting microRNAs using polyphenols may provide a novel strategy for the prevention and treatment of lung cancer.
Article
Biochemistry & Molecular Biology
Long Wang, Xindong Shui, Yingxue Mei, Yongfang Xia, Guihua Lan, Li Hu, Mi Zhang, Chen-Ling Gan, Ruomeng Li, Yuan Tian, Quling Wang, Xi Gu, Dongmei Chen, Tao Zhang, Tae Ho Lee
Summary: This study identified miR-143-3p as a potential candidate for targeting DAPK1 in Alzheimer's disease. miR-143-3p inhibited the translation of DAPK1 and reduced tau phosphorylation, while promoting neurite outgrowth and microtubule assembly. It also decreased amyloid precursor protein phosphorylation and the generation of Aβ. Furthermore, miR-143-3p levels were downregulated and inversely correlated with DAPK1 expression in the hippocampus of AD patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Xin Liu, Jiayu Chen, Wenliang Chen, Yangtao Xu, Yang Shen, Ximing Xu
Summary: RNA-binding proteins play a crucial role in regulating various pathways by binding to RNAs. IGF2BP3, a component of m6A binding proteins, is involved in decoding m6A marks and performing diverse biological functions. Abnormal expression of IGF2BP3 is often associated with poor prognosis in human cancers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Yucui Liang, Mengyu Gu, Xiao Liang, Yueqian Zhou, Qianhua Yang, Zhiwen Wang, Wenbing Yao, Xiangdong Gao, Song Chen
Summary: Parkinson's disease (PD), the second most common neurodegenerative disease, is characterized by selective loss of dopaminergic neurons. In this study, dysregulated levels of von Hippel-Lindau (VHL) and microRNA-143-3p (miR-143-3p) were identified in multiple cell models of PD. miR-143-3p was found to directly bind to VHL mRNA and inhibit its translation, exerting neuroprotective effects by improving cell viability and suppressing apoptosis. Furthermore, miR-143-3p was shown to alleviate mitochondrial abnormalities via the AMPK/PGC-1α axis, suggesting its therapeutic potential for PD.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Cell Biology
Xi Han, Dan Liu, Yuanyuan Zhou, Lijie Wang, Huilian Hou, He Chen, Lirui Zhang, Wei Chen, Xu Li, Le Zhao
Summary: Emerging evidence suggests that miR-143 plays a crucial role in regulating tumor sensitivity to chemotherapeutic agents, particularly in reversing cisplatin resistance in ovarian cancer. The study revealed that miR-143 can inhibit the growth of cisplatin-resistant ovarian cancer cells through downregulating DNMT3A, which is involved in hypermethylation of the miR-143 precursor gene. These findings highlight the important interaction between miR-143 and DNMT3A as a key epigenetic modifier of cisplatin resistance in ovarian cancer.
CELL BIOLOGY INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Chun-Jie Liu, Xin Fu, Mengxuan Xia, Qiong Zhang, Zhifeng Gu, An-Yuan Guo
Summary: SNVs in miRNAs can impact miRNA functions and biogenesis, affecting phenotypes. miRNASNP-v3 is a database with a vast number of SNVs and new features, offering various functions for research on miRNA-related variations.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Orthopedics
Pei Zhang, Jiale Zhang, Huahong Quan, Jingcheng Wang, Yuan Liang
Summary: This study investigated the expression of miR-143 in osteosarcoma and its impact on tumor growth and invasion. The results showed that high levels of miR-143 were associated with reduced tumor volume, increased survival rate, and improvements in lung metastasis and tumor grade. miR-143 may have important value in the treatment and prognosis of osteosarcoma.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Review
Materials Science, Biomaterials
Yi Lin, Ernst Wagner, Ulrich Laechelt
Summary: The CRISPR/Cas technology has become an essential tool in biomedical research, offering hope for therapeutic applications in genomic DNA modification. Delivery strategies for CRISPR/Cas components include plasmid DNA, RNA, and Cas9 ribonucleoproteins. RNA-based delivery has shown promising results in clinical trials, while the translation of ribonucleoprotein delivery to systemic genome editing in patients has not been achieved yet.
BIOMATERIALS SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Victoria C. Vetter, Ernst Wagner
Summary: This review summarizes the challenges and strategies for tumor-targeted nucleic acid delivery, with a focus on polyplexes as nanocarriers and their delivery options across multiple barriers into tumor cells.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Radiology, Nuclear Medicine & Medical Imaging
Carolin Kitzberger, Rebekka Spellerberg, Volker Morath, Nathalie Schwenk, Kathrin A. Schmohl, Christina Schug, Sarah Urnauer, Mariella Tutter, Matthias Eiber, Franz Schilling, Wolfgang A. Weber, Sibylle Ziegler, Peter Bartenstein, Ernst Wagner, Peter J. Nelson, Christine Spitzweg
Summary: The cloning of the sodium iodide symporter (NIS) has opened up new possibilities for gene therapy. Progress has been made in the development of non-viral gene delivery vehicles and monitoring NIS expression using high resolution PET imaging. These advancements have the potential to improve the effectiveness of NIS gene therapy in targeting tumors.
Article
Immunology
Ali Bashiri Dezfouli, Mina Yazdi, Mohamed-Reda Benmebarek, Melissa Schwab, Stefanos Michaelides, Arianna Micciche, Dirk Geerts, Stefan Stangl, Sarah Klapproth, Ernst Wagner, Sebastian Kobold, Gabriele Multhoff
Summary: This study presents a strategy to activate cellular immune response using heat shock protein 70 (Hsp70)-specific antibodies. By using the specific tumor surface antigen mHsp70, NK cells and T cells can be activated to exert cytotoxic effects against tumors. By constructing T cells with a specific anti-Hsp70 CAR, cell lifespan can be prolonged while maintaining their ability to recognize tumor cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Materials Science, Biomaterials
Meng Lyu, Mina Yazdi, Yi Lin, Miriam Hoehn, Ulrich Laechelt, Ernst Wagner
Summary: In this study, an active-targeted dual pH-responsive delivery system was developed for efficient intracellular delivery of protein drugs. The system combines extracellular pH-triggered charge reversal, tumor receptor targeting, and pH-controlled endosomal release to enhance cellular uptake and nuclear localization of the cargo protein.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Review
Chemistry, Multidisciplinary
Ricarda Carolin Steffens, Ernst Wagner
Summary: Nucleic acid therapeutics have emerged as a promising treatment for a variety of diseases, including genetic disorders and cancer. They have also been effectively utilized as vaccines during the COVID-19 pandemic. However, in order to maximize their therapeutic potential, these nano agents must overcome various obstacles, particularly the challenge of targeted transport to specific tissues and cell types.
PHARMACEUTICAL RESEARCH
(2023)
Article
Oncology
Carolin Kitzberger, Rebekka Spellerberg, Yang Han, Kathrin A. Schmohl, Christina Stauss, Christian Zach, Roland E. Kaelin, Gabriele Multhoff, Matthias Eiber, Franz Schilling, Rainer Glass, Wolfgang A. Weber, Ernst Wagner, Peter J. Nelson, Christine Spitzweg
Summary: In this study, genetically engineered MSCs carrying the NIS gene were injected into mice to treat experimental glioblastoma. The results showed that NIS-MSCs successfully homed to the tumor and expressed the NIS gene, and subsequent treatment with therapeutically effective radioiodide (131I) significantly delayed tumor growth and prolonged mouse survival.
CLINICAL CANCER RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Yi Lin, Ulrich Wilk, Jana Poehmerer, Elisa Hoerterer, Miriam Hoehn, Xianjin Luo, Hongcheng Mai, Ernst Wagner, Ulrich Laechelt
Summary: The CRISPR/Cas9 system provides opportunities for precise modification of the genome to treat various diseases. This study presents Cas9 RNP nanocarriers based on lipid-modified oligoamino amides and folic acid (FolA)-PEG for receptor-mediated uptake and gene editing in cancer cells. The nanocarriers achieve efficient knockout of GFP, PD-L1, and PVR genes, and exhibit superior gene editing and tumor growth inhibition compared to non-targeted nanoparticles in an in vivo mouse model.
Article
Chemistry, Multidisciplinary
Sophie Thalmayr, Melina Grau, Lun Peng, Jana Poehmerer, Ulrich Wilk, Paul Folda, Mina Yazdi, Eric Weidinger, Tobias Burghardt, Miriam Hoehn, Ernst Wagner, Simone Berger
Summary: By combining cationizable lipids and polymers, we have developed highly potent pH-responsive nucleic acid carriers for efficient intracellular delivery. These carriers exhibit tunable polarity and structural changes in response to pH, allowing for dynamic nucleic acid delivery. Through optimization of topologies, LAF types, and LAF/aminoethylene ratios, we have identified carriers that are up to several 100-fold more efficient than previous generations and maintain activity in the presence of serum. Importantly, these carriers show excellent in vivo performance, particularly in the spleen, tumor, lungs, and liver.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Paul Strasser, Bianca Montsch, Silvia Weiss, Haider Sami, Christoph Kugler, Sonja Hager, Hemma Schueffl, Robert Mader, Oliver Brueggemann, Christian R. R. Kowol, Manfred Ogris, Petra Heffeter, Ian Teasdale
Summary: The design and synthesis of novel bottlebrush polymers (PPz-g-PGA) with exceptional control over their structure and molecular dimensions have been reported. These polymers show outstanding aqueous solubility, ultra-high multivalency, and biodegradability, making them ideal as nanomedicines. They exhibit enhanced cellular uptake, pharmacokinetics, and tumor accumulation, offering valuable insights into the influence of architecture on the in vivo performance of nanomedicines.
Article
Pharmacology & Pharmacy
Faqian Shen, Yi Lin, Miriam Hoehn, Xianjin Luo, Markus Doeblinger, Ernst Wagner, Ulrich Laechelt
Summary: This study presents a versatile platform for the cellular delivery of various cargo peptides by integrating them into metallo-peptidic coordination nanoparticles. These nanoparticles can enhance the stability and membrane permeability of the peptides, making them efficient in delivering bioactive cargos into cells. The nanoparticles also generate reactive oxygen species (ROS) in cells, further enhancing their therapeutic potential.
Article
Chemistry, Multidisciplinary
Alessio Malfanti, Haider Sami, Anna Balasso, Giulia Marostica, Busra Arpac, Francesca Mastrotto, Giuseppe Mantovani, Elisa Cola, Martina Anton, Paolo Caliceti, Manfred Ogris, Stefano Salmaso
Summary: Studies have shown that liposomes decorated with OCE and PEG have advantages in nucleic acid delivery, improving stability and reducing toxicity. OCE decoration increases liposome adhesion and uptake, while PEG coating reduces hemolysis and cytotoxicity. Treatment with chloroquine can optimize the time window for endo-lysosomal escape.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Yi Lin, Xianjin Luo, Tobias Burghardt, Sarah Dorrer, Miriam Hoehn, Ernst Wagner, Ulrich Laechelt
Summary: This study reports a series of artificial peptides based on novel ionizable amino acids that efficiently deliver Cas9 RNP into cells. The relationship between the xenopeptide logD(7.4) and genome editing potency was revealed through systematic variation of hydrophobic properties. The optimized amphiphilic carriers enable high levels of eGFP knockout and homology-directed repair (HDR) in reporter cells, and mechanistic studies show that hydrophobically balanced xenopeptides are more resistant to stress and promote endocytosis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Polymer Science
Fengrong Zhang, Teoman Benli-Hoppe, Wei Guo, Johanna Seidl, Yi Wang, Rongqin Huang, Ernst Wagner
Summary: Carbon nanodots have great potential in bioimaging applications due to their resistance to photobleaching, bright photoluminescence, and superior biocompatibility. In this study, researchers used four-armed oligomers caged with a novel ligand reL57 to enhance cellular uptake of nanoprobes. The study also highlighted the importance of incorporating epsilon-amino-linked lysines in the development of the oligomers, which significantly extended their contour length per monomer.
Article
Biochemistry & Molecular Biology
Yanira Zeyn, Dominika Hobernik, Ulrich Wilk, Jana Poehmerer, Christoph Hieber, Carolina Medina-Montano, Nadine Roehrig, Caroline F. Straehle, Andrea K. Thoma-Kress, Ernst Wagner, Matthias Bros, Simone Berger
Summary: A deeper understanding of the role of the tumor microenvironment in cancer development has led to the development of new strategies, such as gene-based cancer immunotherapy. This study tested the concept of DC-focused transcriptional targeting by modifying and optimizing plasmids to achieve specific gene expression in antigen-presenting cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)