☆ 4.3 Article

The role of oxidative stress in acrolein-induced DNA damage in HepG2 cells

FREE RADICAL RESEARCH (2008)

Journal

FREE RADICAL RESEARCH

Volume 42, Issue 4, Pages 354-361

Publisher

TAYLOR & FRANCIS LTD

DOI: 10.1080/10715760802008114

Keywords

acrolein; single cell gel electrophoresis; DNA-protein crosslinks; oxidative stress; HepG2 cells

Categories

Biochemistry & Molecular Biology

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Abstract

This study evaluated the role of oxidative stress in acrolein-induced DNA damage, using HepG2 cells. Using the standard single cell gel electrophoresis (SCGE) assay, a significant dose-dependent increment in DNA migration was detected at lower concentrations of acrolein; but at the higher tested concentrations, a reduction in the migration was observed. Post-incubation with proteinase K significantly increased DNA migration in cells exposed to higher concentrations of acrolein. These results indicated that acrolein caused DNA strand breaks and DNA-protein crosslinks (DPC). To elucidate the oxidatively generated DNA damage mechanism, the 2,7-dichlorofluorescein diacetate (DCFH-DA) and o-phthalaldehyde (OPT) were used to monitor the levels of reactive oxygen species (ROS) and glutathione (GSH), respectively. The present study showed that acrolein induced the increased levels of ROS and depletion of GSH in HepG2 cells. Moreover, acrolein significantly caused 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) formation in HepG2 cells. These results demonstrate that the DNA damage induced by acrolein in HepG2 cells is related to the oxidative stress.

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