4.7 Article

Separation and recovery of proteins and sugars from Halloumi cheese whey

Journal

FOOD RESEARCH INTERNATIONAL
Volume 65, Issue -, Pages 477-483

Publisher

ELSEVIER
DOI: 10.1016/j.foodres.2014.03.060

Keywords

Membrane; Ultrafiltration; Nanofiltration; Whey; Protein; Lactose

Ask authors/readers for more resources

Halloumi is the traditional cheese of Cyprus that is produced using typically two processes: milk coagulation and pressed curd cooking. Both processes generate two whey substrates rich in proteins and sugars. The scope of the current study is to investigate the separation and recovery of these compounds by processing samples with a cross-flow ultrafiltration module and five membranes in two sequential steps. Three membranes (100, 50 and 20 kDa) were tested in the first scenario and 20 kDa-permeates were assayed in the second using the two materials with narrower pores of 2 and 1 kDa. Experiments were conducted under constant temperature, circulation flux and several transmembrane pressures, while recovery monitoring was performed by determining operation parameters and retention of proteins, reducing and non-reducing sugars. Results indicated that ultrafiltration is able to separate the target compounds optimally upon two different approaches: using a 100 kDa-polysulphone barrier or by combining 20 kDa-polysulphone and 2 kDa-polyethersulphone membranes. The recovery of proteins in the first approach was high for both samples (69-76%), while the retention of non-reducing sugars (herein expressed mainly lactose) was negligible (2-7%). Using the combined treatment, the recovery of proteins and non-reducing sugars was almost quantitative (87-90%) and rather low (39-32%), respectively. (C) 2014 Elsevier Ltd. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Review Food Science & Technology

The fourth industrial revolution in the food industry-Part I: Industry 4.0 technologies

Abdo Hassoun, Abderrahmane Ait-Kaddour, Adnan M. Abu-Mahfouz, Nikheel Bhojraj Rathod, Farah Bader, Francisco J. Barba, Alessandra Biancolillo, Janna Cropotova, Charis M. Galanakis, Anet Rezek Jambrak, Jose M. Lorenzo, Ingrid Mage, Fatih Ozogul, Joe Regenstein

Summary: Climate change, population growth, food waste, and the risk of new disease outbreaks pose significant challenges to food sustainability and global security. Industry 4.0 technologies have emerged as a vital driver for sustainable development, transforming the food industry and offering solutions to these challenges. However, groundbreaking sustainable solutions can only be achieved by combining multiple technologies simultaneously.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2023)

Article Environmental Sciences

Bioeconomy and green recovery in a post-COVID-19 era

Charis M. Galanakis, Gianluca Brunori, David Chiaramonti, Robert Matthews, Calliope Panoutsou, Uwe R. Fritsche

Summary: This article discusses how the bioeconomy can enhance the resilience and sustainability of biobased, food, and energy systems in the post-COVID-19 era by integrating technological innovations, environment, culture, arts, and other sectors to promote economic and social innovations.

SCIENCE OF THE TOTAL ENVIRONMENT (2022)

Article Food Science & Technology

Sustainable Applications for the Valorization of Cereal Processing By-Products

Charis M. Galanakis

Summary: This review article examines the sustainable practices and applications of valorizing valuable components from cereal processing by-products. The article focuses on the healthy compounds and functional properties of these by-products and explores reutilization opportunities and extraction methods. Additionally, it discusses the various applications of the recovered compounds.

FOODS (2022)

Article Chemistry, Applied

Isolation and characterisation of milk-derived amyloid-like protein aggregates (MAPA) from cottage cheese

Shweta Malik, Indranil De, Manish Singh, Charis M. Galanakis, Abdulhakeem S. Alamri, Jay Kant Yadav

Summary: This study found that the heat treatment and subsequent acidification during cottage cheese preparation lead to the formation of non-toxic amyloid-like aggregates.

FOOD CHEMISTRY (2022)

Article Biotechnology & Applied Microbiology

Salmonella Enteritidis survival in different temperatures and nutrient solution pH levels in hydroponically grown lettuce

Panayiota Xylia, Antonios Chrysargyris, George Botsaris, Panagiotis Skandamis, Nikolaos Tzortzakis

Summary: The study investigated the effects of different plant growth stages, pH values, and bacterial inoculum levels on hydroponically cultivated lettuce spiked with Salmonella Enteritidis. pH and inoculum levels were found to affect the internalization and survival of the pathogen in the hydroponic environment and plant tissue. Younger plants were more susceptible to pathogen internalization, and despite bacterium presence in the hydroponic solution, no leaf internalization was observed at all lettuce growth stages.

FOOD MICROBIOLOGY (2022)

Review Food Science & Technology

Carica papaya biowaste valorization: Biorefinery advances and extraction optimization

Sherif Shaheen, Charis M. Galanakis, Mohamed A. Farag

Summary: Papaya is a tropical fruit known for its nutritional value and health benefits. The seeds and peels of papaya, which account for 20% of the fruit weight, contain valuable chemical constituents such as glucosinolates, lipids, proteins, and dietary fibers. This review outlines the composition of papaya fruit biowastes, their biological functions, and their applications in functional food, bioenergy, and other industries.

FOOD REVIEWS INTERNATIONAL (2023)

Article Chemistry, Multidisciplinary

Green and highly extraction of phenolic compounds and antioxidant capacity from kinkeliba (Combretum micranthum G. Don) by natural deep eutectic solvents (NADESs) using maceration, ultrasound-assisted extraction and homogenate-assisted extraction

Oscar Zannou, Hojjat Pashazadeh, Salam A. Ibrahim, Ilkay Koca, Charis M. Galanakis

Summary: In this study, natural deep eutectic solvents were used for the extraction of phenolic compounds from dried Kinkeliba leaves. The results showed that acidic deep eutectic solvents, especially combined with ultrasound-assisted or homogenate-assisted extraction, achieved higher extraction efficiency.

ARABIAN JOURNAL OF CHEMISTRY (2022)

Review Food Science & Technology

Truths and myths about superfoods in the era of the COVID-19 pandemic

Abdo Hassoun, Rania Harastani, Sandeep Jagtap, Hana Trollman, Guillermo Garcia-Garcia, Nour M. H. Awad, Oscar Zannou, Charis M. Galanakis, Gulden Goksen, Gulzar Ahmad Nayik, Asad Riaz, Sajid Maqsood

Summary: During the COVID-19 pandemic, consumers are seeking foods that not only satisfy hunger but also enhance health and well-being. This has led to increased attention on superfoods, which claim to be rich in valuable nutrients and have the ability to prevent illness and improve overall health. However, scientific evidence for the effectiveness of superfoods is still limited. More research and collaboration are needed to differentiate facts from marketing gimmicks and promote human health and nutrition.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2022)

Article Plant Sciences

Non-ionic surfactants-mediated green extraction of polyphenols from red grape pomace

Darija Sazdanic, Milica A. Krstonosic, Dejan Cirin, Jelena Cvejic, Abdulhakeem Alamri, Charis M. Galanakis, Veljko Krstonosic

Summary: This study investigated the potential of eleven non-ionic surfactants belonging to poloxamer, Brij, Triton, and Tween subgroups for the extraction of polyphenols from red grape pomace. The results showed that surfactants with longer oxyethylene chains provided greater efficiency (Brij S20 and poloxamer 407). It was also found that the chemical structure of surfactants influenced the solubilization mechanism of polyphenols.

JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS (2023)

Article Chemistry, Multidisciplinary

Exploring the Amino-Acid Composition, Secondary Structure, and Physicochemical and Functional Properties of Chickpea Protein Isolates

Sumeyra Onder, Asli Can Karaca, Beraat Ozcelik, Abdulhakeem S. Alamri, Salam A. Ibrahim, Charis M. Galanakis

Summary: This study analyzed the amino-acid profile, secondary structure, and physicochemical and functional properties of proteins from Anatolian chickpea landraces. The study also aimed to determine the relationship between amino-acid composition and physicochemical and functional properties. The results showed that aspartic acid and glutamic acid were the dominant amino acids, and the proteins lacked methionine. The dominant secondary structure was beta-sheet, and the isolates exhibited different properties such as denaturation temperature, surface charge, solubility, water holding capacity, oil-holding capacity, emulsion capacity, emulsifying activity, and stability indices.

ACS OMEGA (2023)

Article Green & Sustainable Science & Technology

Exploring the Potential of Halloumi Second Cheese Whey for the Production of Lactic Acid Cultures for the Dairy Industry

Eleni Naziri, Eugenia Papadaki, Iordanis Savvidis, George Botsaris, Konstantinos Gkatzionis, Ediriisa Mugampoza, Fani Th. Mantzouridou

Summary: The production of halloumi cheese in Cyprus has been increasing rapidly due to its growing popularity, resulting in excess production of whey streams that pose environmental concerns. In this study, the second cheese whey (SCW) generated during the extraction of anari, a by-product of halloumi cheese, was investigated for the production of dairy industry lactic acid bacteria (LAB) culture. The LAB isolated from SCW were molecularly identified and characterized, and their potential use as starter cultures for the dairy industry was explored. Different species of LAB demonstrated species-specific differences in galactose and lactose catabolism, and culturing the selected isolates in SCW supplemented with skimmed milk improved their freeze-drying tolerance and viability during storage.

SUSTAINABILITY (2023)

Article Food Science & Technology

The Vertigo of the Food Sector within the Triangle of Climate Change, the Post-Pandemic World, and the Russian-Ukrainian War

Charis M. M. Galanakis

Summary: In recent years, the world has faced multiple crises, including climate change, the COVID-19 pandemic, and the Russian-Ukrainian war, which have had a significant impact on the food sector. To address the challenges posed by these crises, it is crucial to transform food systems and increase their resilience and sustainability. This requires the cooperation and involvement of all actors within the supply chain, as well as the adoption of proactive measures such as food safety, circular economy principles, digitalization, and inclusivity. Modernizing food production and developing shorter, domestic supply chains are also key to achieving food resilience and security.

FOODS (2023)

Article Veterinary Sciences

Knowledge and Practices of Cypriot Bovine Farmers towards Effective and Safe Manure Management

Soteris Christophe, Kristina Pentieva, George Botsaris

Summary: The study aims to evaluate the knowledge and practices of Cypriot bovine farmers towards safer manure management and highlights the importance of providing relevant training to farmers to ensure proper manure management. Interventions to promote the use of more effective treatment methods would be beneficial in decreasing manure pathogens.

VETERINARY SCIENCES (2023)

Article Multidisciplinary Sciences

Preparation, optimization and evaluation of Osthole transdermal therapeutic system

Muhammad Naeem, Taniya Iqbal, Muhammad Yousuf, Zarqa Nawaz, Sajjad Hussain, Abdulhakeem S. Alamri, Charis M. Galanakis, Atif Ali

Summary: In this study, the solubility and permeability of Osthole-loaded microemulsion were enhanced, leading to increased bioavailability. The microemulsion preparation demonstrated good stability and non-irritating properties, making it a promising carrier for transdermal delivery of Osthole.

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2023)

Article Food Science & Technology

Creating new opportunities for sustainable food packaging through dimensions of industry 4.0: New insights into the food waste perspective

Abdo Hassoun, Fatma Boukid, Fatih Ozogul, Abderrahmane Ait-Kaddour, Jose Miguel Soriano, Jose M. Lorenzo, Rosa Perestrelo, Charis M. Galanakis, Gioacchino Bono, Abdelhakim Bouyahya, Zuhaib Bhat, Slim Smaoui, Anet Rezek Jambrak, Jose S. Camaraj

Summary: This review discusses the interplay between food packaging, food waste, and sustainability. It briefly summarizes common strategies used in food packaging and emphasizes the significance of nanobiotechnology, smart sensors, and other Industry 4.0 innovations. Consumer acceptance of novel packaging materials/technologies is also mentioned, and conclusions are drawn.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2023)

Article Food Science & Technology

Analysis of aroma components changes in Gannan navel orange at different growth stages by HS-SPME-GC-MS, OAV, and multivariate analysis

Yonghong Ye, Songyan Zheng, Yuanxing Wang

Summary: In this study, the changes of aroma components in Gannan navel orange during growth were systematically studied using HS-SPME-GC-MS. Key aroma components and markers were identified. These findings contribute to a better understanding of the dynamic variation of aroma compounds during navel orange growth and have potential for industrial applications.

FOOD RESEARCH INTERNATIONAL (2024)

Article Food Science & Technology

Effects of foliar selenium application on Se accumulation, elements uptake, nutrition quality, sensory quality and antioxidant response in summer-autumn tea

Jia Xu, Yayuan Zhang, Mengke Zhang, Xinlin Wei, Yiming Zhou

Summary: This study evaluated the effects of glucosamine selenium on summer-autumn tea, showing that it can enhance nutritional quality, improve sensory characteristics, and increase plant adaptation to environmental changes and abiotic stresses.

FOOD RESEARCH INTERNATIONAL (2024)

Article Food Science & Technology

The application of ice glazing containing D-sodium erythorbate combined with vacuum packaging to maintain the physicochemical quality and sweet/umami non-volatile flavor compounds of frozen stored large yellow croaker (Pseudosciaena crocea)

Yuanming Chu, Zhaoyang Ding, Jing Xie

Summary: This study investigated the use of ice glazing containing D-sodium erythorbate (DSE) and vacuum packaging to maintain the quality of large yellow croaker during frozen storage. The results showed that vacuum packaging effectively inhibited ice crystal growth and minimized water loss, while the combination of vacuum packaging and 0.3% DSE-infused ice glazing maintained freshness indicators at low levels throughout the 300-day storage period. This combination was considered the most effective in preserving the quality of the fish.

FOOD RESEARCH INTERNATIONAL (2024)

Review Food Science & Technology

Insights into the mechanisms and key factors influencing biofilm formation by Aeromonas hydrophila in the food industry: A comprehensive review and bibliometric analysis

Md. Ashikur Rahman, Shirin Akter, Md. Ashrafudoulla, Md. Anamul Hasan Chowdhury, A. G. M. Sofi Uddin Mahamud, Si Hong Park, Sang-Do Ha

Summary: This comprehensive review aims to provide insights into the mechanisms and key factors influencing biofilm formation by A. hydrophila in the food industry. It explores the molecular processes involved in various stages of biofilm formation and investigates the impact of intrinsic factors and environmental conditions on biofilm architecture and resilience. The article also highlights the potential of bibliometric analysis in conceptualizing the research landscape and identifying knowledge gaps in A. hydrophila biofilm research.

FOOD RESEARCH INTERNATIONAL (2024)

Article Food Science & Technology

Effects of solid lipid ratio in curcumin loaded emulsions on its gastrointestinal fate: Colloidal stability and mucus absorption efficiency

Chujing Wang, Wenni Tian, Zengliu Song, Qun Wang, Yong Cao, Jie Xiao

Summary: Solid lipid ratio in emulsions has significant effects on colloidal stability, mucus permeability, and bioavailability in vivo. Higher solid lipid ratio improves intestinal stability but reduces mucus permeability.

FOOD RESEARCH INTERNATIONAL (2024)

Article Food Science & Technology

Development of sequential online extraction electrospray ionization mass spectrometry for accurate authentication of highly-similar Atractylodis Macrocephalae

Dacai Zhong, Liping Kang, Juan Liu, Xiang Li, Li Zhou, Luqi Huang, Zidong Qiu

Summary: In this study, a novel online extraction electrospray ionization mass spectrometry method was developed to comprehensively characterize complex foods. Meanwhile, a characteristic marker screening method and chemometrics modeling were used for the accurate authentication of highly-similar foods. This research has significant implications for ensuring food quality and safety.

FOOD RESEARCH INTERNATIONAL (2024)

Article Food Science & Technology

Hypoglycemic effect and intestinal transport of phenolics-rich extract from digested mulberry leaves in Caco-2/insulin-resistant HepG2 co-culture model

Qiannan Zhao, Jinyi Yang, Jiahui Li, Lei Zhang, Xiaohai Yan, Tianli Yue, Yahong Yuan

Summary: This study investigated the transport and hypoglycemic effects of phenolics in mulberry leaves using an in vitro digestion model. The results showed that digested phenolics had higher absorbability and could inhibit glucose digestion and absorption. Phenolics also regulated glucose metabolism. Luteoforol and p-coumaric acid were found to be the primary phenolics strongly correlated with the hypoglycemic ability of mulberry leaves.

FOOD RESEARCH INTERNATIONAL (2024)

Article Food Science & Technology

Modulating in vitro digestion of whey protein cold-set emulsion gels via gel properties modification with gallic acid and EGCG

Yanyun Cao, Qingling Wang, Jinou Lin, Yin-Yi Ding, Jianzhong Han

Summary: This study investigated the effects of gallic acid (GA) and epigallocatechin gallate (EGCG) at different ratios on the gel properties of calcium induced-whey protein emulsion gel. It was found that GA and EGCG could promote gel formation, increase gel strength, and delay the release of emulsified oil droplets.

FOOD RESEARCH INTERNATIONAL (2024)

Article Food Science & Technology

Simultaneous extraction, separation, and analysis of 5-caffeoylquinic acid and caffeine from coffee co-product by PLE-SPE x HPLC-PDA two-dimensional system

Monique Martins Strieder, Vitor Lacerda Sanches, Mauricio Ariel Rostagno

Summary: This study proposed an integrated and automated procedure for extracting, separating, and quantifying bioactive compounds from coffee co-products. By optimizing the extraction, separation, and analysis method, real-time analysis was achieved. The results suggest that coupling different techniques can efficiently extract, separate, and analyze phenolic compounds, providing an integrated method to produce high-added value ingredients for various applications.

FOOD RESEARCH INTERNATIONAL (2024)

Article Food Science & Technology

Geographical origin discriminatory analysis of onions: Chemometrics methods applied to ICP-OES and ICP-MS analysis

Kyu Sang Sim, Hyoyoung Kim, Suel Hye Hur, Tae Woong Na, Ji Hye Lee, Ho Jin Kim

Summary: Geographical origin plays a crucial role in determining the quality and safety of agricultural products. In this study, ICP analysis was used to determine the inorganic elemental content of onions and identify their geographical origin. Chemometric methods were applied to analyze the ICP results, and the accuracy of distinguishing between Korean and Chinese onions was found to be excellent. The findings suggest that this method can be beneficial for identifying agricultural products.

FOOD RESEARCH INTERNATIONAL (2024)