Article
Engineering, Environmental
Xianbo Chang, Yuanyuan He, Lehui Song, Jing Ding, Suyu Ren, Min Lv, Lingxin Chen
Summary: Microalgae Chlorella vulgaris and Phaeodactylum tricornutum have the ability to degrade methylparaben (MPB), with Chlorella vulgaris showing higher efficiency and rate of degradation, and the ability to completely mineralize and detoxify MPB.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Ting-Bin Hao, Yang Lu, Zhong-Hong Zhang, Si-Fen Liu, Xiang Wang, Wei-Dong Yang, Srinivasan Balamurugan, Hong-Ye Li
Summary: This study successfully improved carotenoid production in diatom Phaeodactylum tricornutum through genetic modification, particularly by overexpressing key carotenogenic genes involved in the MEP pathway. The elevated expression of carotenogenic genes led to increased total carotenoid content, with significant enhancement in fucoxanthin content in the engineered lines CMK and CMS.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Min Wang, Angeles Moron-Ortiz, Jianjun Zhou, Ana Benitez-Gonzalez, Paula Mapelli-Brahm, Antonio J. Melendez-Martinez, Francisco J. Barba
Summary: This study investigated the impact of pressurized liquid extraction (PLE) and DMSO concentration on the yield of antioxidants and minerals from Chlorella. The results showed that PLE increased the antioxidant yield, with 100% DMSO obtaining more polyphenols, chlorophylls, and carotenoids. PLE + 100% DMSO was found to be the most suitable method for the recovery of antioxidants and pigments from Chlorella. The extracts were also found to be a good source of minerals for different populations.
Article
Biotechnology & Applied Microbiology
Sergio Sousa, Ana C. Freitas, Ana M. Gomes, Ana P. Carvalho
Summary: Temperature and light intensity were independently used as stress modulators to enhance EPA production by Nannochloropsis oculata. The study found that temperature stress had the highest impact on EPA content, while lower light intensity stress also increased EPA production significantly.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Giovanna Salbitani, Francesco Del Prete, Simona Carfagna, Giovanni Sansone, Carmela M. A. Barone
Summary: The study demonstrates that the addition of bicarbonate to Nannochloropsis oculata cultures can significantly enhance algal growth and pigment contents, especially at higher concentrations. Bicarbonate from 30 to 60 mM can rapidly improve the cellular levels of chlorophylls and carotenoids in a short period of time, providing a sustainable opportunity to improve microalgae cultivation.
Article
Biotechnology & Applied Microbiology
Sergio Sousa, Ana P. Carvalho, Carlos A. Pinto, Renata A. Amaral, Jorge A. Saraiva, Ricardo N. Pereira, Antonio A. Vicente, Ana C. Freitas, Ana M. Gomes
Summary: To make Nannochloropsis oculata a financially viable source for commercial applications, extraction efficiency needs to be improved. This study tested emerging technologies such as high hydrostatic pressure (HHP) and moderate electric fields (MEF), along with solvents with different polarity indexes, to increase accessibility and extraction yields of eicosapentaenoic acid (EPA). The combined use of HHP and MEF resulted in a 62% increase in EPA extraction yields from wet N. oculata biomass. These findings are highly relevant for the food and pharmaceutical industries, providing alternatives to traditional extraction methods with increased yields and reduced environmental impact.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Bing Li, Di Wu, Yan Li, Yan Shi, Chenlin Wang, Jiasi Sun, Chunfeng Song
Summary: Antibiotic resistance is a significant environmental challenge, and the use of microalgae for environmental purification shows great potential, particularly in the degradation of sulfadimethoxine.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Yan Sun, Yi Xin, Luyao Zhang, Ying Wang, Ruolan Liu, Xiaohui Li, Chengxu Zhou, Lin Zhang, Jichang Han
Summary: This study successfully increased the violaxanthin content in Nannochloropsis by transforming PtCRTISO4, and found that PtCRTISO4 played a regulatory role in carotenoid accumulation and also affected fatty acid content. These findings provide valuable information for the production of violaxanthin from Nannochloropsis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tomas Grivalsky, Antonin Strizek, Pavel Pribyl, Jaromir Lukavsky, Radim Cegan, Roman Hobza, Pavel Hrouzek
Summary: Detection of fast-growing green algae Chlorella vulgaris as a contaminant in diatom Phaeodactylum tricornutum cultures using various approaches reveals PCR/qPCR as the most reliable and sensitive method with a detection sensitivity close to 75 cells/mL. This method is effective even in mixed cultures containing a large number of P. tricornutum cells. The cultivation media exchange method from sea water to fresh water is similarly sensitive to PCR approaches for detecting contaminants, albeit with a longer detection time.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Sergio C. Sousa, Manuela Machado, Ana C. Freitas, Ana M. Gomes, Ana P. Carvalho
Summary: Research has shown that the lipid extracts of Nannochloropsis oculata can enhance their health benefits by modulating temperature, exhibiting various biological activities such as antioxidant, anti-inflammatory, adipolytic, and anti-steatosis effects.
Article
Food Science & Technology
Joana Matos, Claudia Afonso, Carlos Cardoso, Maria L. Serralheiro, Narcisa M. Bandarra
Summary: Microalgae, such as Isochrysis galbana, are being explored as a valuable source of natural nutrients and bioactive compounds for functional food applications. This study demonstrated that incorporating I. galbana biomass and lipid extract into yogurt can significantly increase the omega 3 LC-PUFAs content, particularly DHA, making it a potentially important nutritional source. Further research is needed to confirm the bioavailability of these compounds in vivo.
Article
Fisheries
Shigeki Dan, Kazuhiro Yamashita, Hiroyuki Matsunari, Kosuke Nagakura, Katsuyuki Hamasaki
Summary: The study investigates the ability of Artemia to digest microalgae and finds that it acquires the ability to consume them in a step-by-step manner depending on its growth and the species of microalgae.
AQUACULTURE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Aisamuddin Ardi Zainal Abidin, Chotika Yokthongwattana, Zetty Norhana Balia Yusof
Summary: The study investigated the impact of salinity and oxidative stress on carotenoid biosynthesis in Nannochloropsis oculata. The results showed that both stressors upregulated carotenoid biosynthesis genes and resulted in the overexpression of targeted carotenoids. This insight is beneficial for understanding microalgae responses to abiotic stress via carotenoid synthesis.
Article
Biotechnology & Applied Microbiology
Danae Samara Sanchez-Sandoval, Omar Gonzalez-Ortega, Juan Vazquez-Martinez, Ramon Fernando Garcia de la Cruz, Ruth Elena Soria-Guerra
Summary: In this study, different microalgae were evaluated for their efficiency in removing diclofenac. The results showed that S. obliquus was the best alternative for bioremediation of diclofenac.
Article
Biochemistry & Molecular Biology
Maria D. Gkioni, Vasilis Andriopoulos, Eleni Koutra, Sophia Hatziantoniou, Michael Kornaros, Fotini N. Lamari
Summary: The aim of this study was to develop an efficient green extraction method for Nannochloropsis oculata to produce antioxidant extracts and nutritious residual biomass. Among the 21 extraction methods evaluated, ultrasound-assisted extraction with ethanol or betaine: 1,2-propanediol in a molar ratio of 2:5 showed optimal extractive capacity. The ethanol extract exhibited higher antioxidant activity and contained higher concentrations of violaxanthin and antheraxanthin. The use of betaine: 1,2-propanediol caused less depletion of lipids from the biomass compared to ethanol extraction, but resulted in higher content of proteins, carbohydrates, and ash in the extracted biomass.
Article
Engineering, Environmental
Zhenyu Zhao, Koenraad Muylaert, Anthony Szymczyk, Ivo F. J. Vankelecom
Summary: This study highlights the significant impact of membrane charge and surface pattern on membrane filtration performance, especially in terms of microalgae harvesting efficiency. By optimizing the membrane pattern and charge, it is possible to enhance the clean water permeance and microalgae harvesting efficiency.
Article
Agriculture, Multidisciplinary
Robbe Demets, Lore Bonneux, Celine Dejonghe, Lore Gheysen, Ann Van Loey, Imogen Foubert
Summary: This study investigated the oxidative impact of illumination on photoautotrophic microalgae and heterotrophic microalgae during storage, and found that photo-oxidation had minimal effect on the oxidative stability of photoautotrophic microalgae, while causing strong autoxidation in heterotrophic microalgae.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Robbe Demets, Simon Van Broekhoven, Lore Gheysen, Ann Van Loey, Imogen Foubert
Summary: Fish oil is an important source of health-beneficial omega-3 long-chain polyunsaturated fatty acids, but it is susceptible to lipid oxidation. This research investigates the potential of Phaeodactylum carotenoids as antioxidants for fish oil. It was found that the ratio of carotenoids to n-3 LC-PUFA content determines the oxidative stability of the fish oil.
Article
Environmental Sciences
Karen Portilla, Elizabeth Velarde, Ellen Decaestecker, Franco Teixeira de Mello, Koenraad Muylaert
Summary: Submerged macrophytes promote water clarity in high-altitude Andean shallow lakes by reducing the cyanobacteria bloom and increasing zooplankton grazing. In a series of experiments in Yahuarcocha Lake, it was found that the presence of Egeria densa resulted in a strong reduction in cyanobacteria, while the small-sized fish Poecilia reticulata promoted cyanobacteria dominance. The combination of E. densa, fish, and zooplankton substantially reduced the algae, indicating the important role of submerged plants in improving water quality in high-elevation eutrophic ecosystems.
Article
Agricultural Engineering
N. R. H. Rao, V. P. Beyer, R. K. Henderson, W. Thielemans, K. Muylaert
Summary: Flocculation combined with dissolved air flotation (DAF) is a promising technology for harvesting microalgae, and the optimization of flocculant-DAF operating conditions is frequently explored in laboratory experiments. The aim of this study was to systematically compare the harvesting efficiency (eta) between different types of benchtop DAF jars. Evaluation of 30 different types of DAF jars revealed that the eta was not influenced by the volume of the jars, but was impacted by the height to diameter ratio. These results provide useful guidance for the design of lab-scale DAF microalgae harvesting experiments.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Applied
Robbe Demets, Lore Gheysen, Ann Van Loey, Imogen Foubert
Summary: This research investigates the role of carotenoids in the oxidative stability of mixtures of fish oil and lipid extracts of two different microalgae. It finds that the initial amount of carotenoids relative to n-3 LC-PUFA is a good indicator to differentiate between oxidatively stable and unstable mixtures. The degradation of fucoxanthin, diatoxanthin, and beta-carotene during storage illustrates their antioxidative role.
Article
Chemistry, Applied
Eline Van Wayenbergh, Jolien Verheijen, Niels A. Langenaeken, Imogen Foubert, Christophe M. Courtin
Summary: In this study, a new method for analyzing retinyl palmitate without the need for saponification was developed and validated. The results showed that the improved extraction protocols had low limits of detection and quantification, as well as good recovery and precision for retinyl palmitate extraction from model systems. For complex food matrices, the chloroform/methanol extraction protocol demonstrated the best performance characteristics.
Article
Biotechnology & Applied Microbiology
N. R. H. Rao, A. Gonzalez-Torres, B. Tamburic, Y. W. Wong, I. Foubert, K. Muylaer, R. K. Henderson, D. Vandamme
Summary: Flocculation in marine microalgae can be induced sustainably through alkaline flocculation, resulting in flocs that can be harvested via dissolved air flotation (DAF) or sedimentation. Though DAF provides faster separation and higher solids content compared to sedimentation, its effectiveness in saline environments when used with alkaline flocculation has not been tested. This study evaluates the impact of alkaline floc properties on DAF performance and compares it with sedimentation and ferric chloride flocculation.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Food Science & Technology
Esther Mienis, Dries Vandamme, Imogen Foubert
Summary: This review provides an overview of recent literature on enzyme-assisted disruption (EAD) of Nannochloropsis to increase lipid extraction yield. The selection of enzymes and solvents, as well as treatment conditions, vary considerably in the literature. The aim is to provide guidelines and good practices for enzyme selection, pre-treatment, and post-treatment that can be applied to other oleaginous microalgae.
CURRENT OPINION IN FOOD SCIENCE
(2023)
Article
Food Science & Technology
Robbe Demets, Lore Gheysen, Ann Van Loey, Imogen Foubert
Summary: Microalgae rich in omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) have the potential for developing functional food and offer a sustainable alternative to fish stock. The presence of endogenous carotenoids in microalgae contributes to the oxidative stability of enriched products. However, further research is needed to understand the antioxidative role of carotenoids and its impact on selecting microalgae for enrichment.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Eline Van Wayenbergh, Niels A. Langenaeken, Nore Struyf, Peter Goos, Imogen Foubert, Christophe M. Courtin
Summary: Food fortification is an effective strategy against vitamin A deficiency, and cereal bran can be used as a natural stabilising agent. The stabilising effect of wheat bran on vitamin A is stronger for samples with high antioxidant capacity, high bound lipid content, and low lipase activity. Wheat bran antioxidants and bound lipids protect vitamin A from degradation during storage, while endogenous lipase activity counteracts the stabilising effect.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Marine & Freshwater Biology
Karen Portilla, Elizabeth Velarde, Tania Ona, Ellen Decaestecker, Franco Teixeira de Mello, Koenraad Muylaert
Summary: This study investigated the behavioral response of the local Daphnia pulex in the high-elevation shallow Lake Yahuarcocha in Peru to an exotic submerged macrophyte and an exotic fish. The results showed that Daphnia pulex strongly avoided the macrophyte, indicating that it does not provide refuge from fish predation.
Article
Food Science & Technology
Hannes Withouck, Axel Paelinck, Imogen Foubert, Ilse Fraeye
Summary: This study is focused on the extraction of polyphenols from applewood using ultrasonic-assisted extraction (UAE). The influence of solvent composition and mass-volume (m:v) ratio on the extraction yield was investigated at both lab scale (200 mL) and pilot scale (100 L). Higher yields of polyphenols were obtained with certain conditions, such as a ratio of 1:33 (m:v) and a presence of 30 v/v % ethanol mixture. The UAE technique showed a significantly higher yield compared to conventional solid-liquid extraction technique.
Meeting Abstract
Chemistry, Applied
Eline Van Wayenbergh, Christopher M. Courtin, Niels A. Langenaeken
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2022)
Meeting Abstract
Chemistry, Applied
Esther Mienis, Foubert Imogen
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2022)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
