4.7 Article

Interaction between lysozyme and procyanidin: Multilevel structural nature and effect of carbohydrates

Journal

FOOD CHEMISTRY
Volume 138, Issue 2-3, Pages 1596-1603

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.11.027

Keywords

Procyanidins; Lysozyme; Aggregation; Fluorescence; Carbohydrates

Funding

  1. Natural Science Foundation of China [20806057, 31071509]
  2. Ministry of Science and Technology of China [2012BAD29B05, 2012YQ090194, 2012AA06A303]
  3. Ministry of Education [B06006, NCET-11-0372]

Ask authors/readers for more resources

The interaction of procyanidins with proteins has aroused extensive attention due to its important relationship with the bioavailability and astringent property of polyphenols. In the present work, we have investigated the interactions of lysozyme with procyanidin dimer (B3) using various biophysical approaches, which aims to provide insights into the mechanism of protein/polyphenol aggregation. Procyanidin B3 spontaneously binds lysozyme, inducing the multilevel structural changes in lysozyme and the formation of insoluble complexes. The relationship between lysozyme aggregation and the loss of enzymatic activity was monitored using dynamic light scattering and fluorescence quenching. The influences of two carbohydrates (gum arabic and sucrose) on lysozyme/B3 aggregation were also studied. Gum arabic effectively inhibited the formation of insoluble aggregates, but was unable to restore the fluorescence and activity of lysozyme. However, sucrose concomitantly decreased the aggregate size with the recovery of fluorescence and lysozyme activity. These results proposed two probable mechanisms by which these two carbohydrates inhibit protein/polyphenol aggregation. (C) 2012 Elsevier Ltd. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Review Food Science & Technology

Recent progress in oil-in-water-in-oil (O/W/O) double emulsions

Zijian Zhi, Rui Liu, Wenjun Wang, Koen Dewettinck, Filip Van Bockstaele

Summary: This review summarizes the research advances of O/W/O double emulsions over the past two decades, focusing on preparation strategies, stabilization mechanism, and potential applications. Various emulsification strategies, interfacial stabilizers, and viscosity effects on stability are discussed. Analytical methods for evaluating stability and possible innovative food applications are also highlighted.

CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION (2023)

Article Chemistry, Physical

Lubricants for osteoarthritis treatment: From natural to bioinspired and alternative strategies

Hui Yuan, Laura L. E. Mears, Yuefei Wang, Rongxin Su, Wei Qi, Zhimin He, Markus Valtiner

Summary: Osteoarthritis, a common degenerative joint disease, causes serious pain due to the progressive loss and destruction of articular cartilage. Restoring joint lubrication is crucial for its treatment, and various lubricants have been developed to reduce friction and promote cartilage repair. This review discusses the recent progress in natural, bioinspired, and alternative lubricants for osteoarthritis treatment, as well as their structural characterization and lubrication properties.

ADVANCES IN COLLOID AND INTERFACE SCIENCE (2023)

Review Chemistry, Multidisciplinary

Biomimetic mineralization based on self-assembling peptides

Qing Li, Yuefei Wang, Gong Zhang, Rongxin Su, Wei Qi

Summary: Biomimetic science has attracted significant interest in various fields, including chemistry, biology, materials science, and energy. Biomimetic mineralization involves the synthesis of inorganic minerals under the control of organic molecules or biomolecules, with peptides playing a crucial role as building blocks. Peptides can precisely control the morphology, size, and composition of mineralized materials, and also provide templates for the nucleation and growth of inorganic nanomaterials, offering potential for high catalytic efficiency, selectivity, and biotherapeutic functions.

CHEMICAL SOCIETY REVIEWS (2023)

Article Environmental Sciences

Efficient removal of Phaeocystis globosa from seawater with the persulfate activation by arbutin-modified cellulose nanocrystals

Mingda Che, Cancan Shan, Wenjie Zhang, Yanyi Duan, Renliang Huang, Mei Cui, Wei Qi, Rongxin Su

Summary: A novel heterogenous Fenton-like process utilizing arbutin-modified cellulose nanocrystals (AT-CNC) activated persulfate was proposed to remove the harmful algal blooms (HABs) Phaeocystis globosa from seawater. The results showed that the removal efficiency of P. globosa reached high levels under certain additive conditions. Therefore, this method has the potential to be an efficient option for controlling HABs.

CHEMOSPHERE (2023)

Article Chemistry, Multidisciplinary

Hierarchical metal-peptide assemblies with chirality-encoded spiral architecture and catalytic activity

Jiayu Liu, Jiaxing Zhang, Liwei Zhang, Yuefei Wang, Hao Wei, Yuhe Shen, Jiwei Min, Xi Rong, Wei Qi, Rongxin Su, Zhimin He

Summary: This study reports the coordination assembly of ferrocene-diphenylalanine (Fc-FF) with divalent copper ions (Cu2+), resulting in the formation of metal-peptide assemblies (MPAs) with hierarchical spiral architectures. The MPAs consist of helically organized nanofibers that resemble logarithmic spirals. These MPAs exhibit hierarchically porous structures with abundant Fc and Cu2+ active sites, displaying significantly higher catalytic activity than natural laccase in decolorization reactions. Furthermore, the temperature and enantiomeric excess (ee) can be controlled to synthesize a series of hierarchical structures for MPAs. Peptide enantiomers with higher ee values self-assemble into highly complex and ordered structures, which exhibit higher surface area and porosity and thus enhanced catalytic activity compared to those assembled by peptides with lower ee values. These findings provide new insights into the crucial role of chirality in directing the self-assembly of biomolecules into highly ordered complex functional structures.

SCIENCE CHINA-CHEMISTRY (2023)

Article Food Science & Technology

Identification of a Novel Walnut Iron Chelating Peptide with Potential High Antioxidant Activity and Analysis of Its Possible Binding Sites

Chaozhong Fan, Xintong Wang, Xiwang Song, Ronghao Sun, Rui Liu, Wenjie Sui, Yan Jin, Tao Wu, Min Zhang

Summary: A new type of walnut peptide iron (WP-Fe) chelate with high iron-binding capacity and antioxidant activity was prepared. The WP-Fe chelate exhibited a different morphological structure and stronger free radical scavenging ability compared to WP. Peptide iron chelates are expected to become a promising source of iron supplement and antioxidant activities.

FOODS (2023)

Article Biotechnology & Applied Microbiology

A reusable and leakage-proof immobilized laccase@UiO-66-NH2(30) for the efficient biodegradation of rifampicin and lincomycin

Chang Liu, Xiaoyue Zhang, Yu Zhou, Lin Zhu, Chengyu Zhang, Xiaohui Yan, Shengping You, Wei Qi, Rongxin Su

Summary: A leakage-proof Zr-based metal-organic framework (MOF) carrier, UiO-66NH2(30), was constructed to immobilize laccase (Lac) and enhance antibiotic biodegradation. The Lac was fixed inside the carrier with a high loading amount (275.96 mg/g) and recovery (618%). The immobilized Lac@UiO-66-NH2(30) exhibited excellent reusability and stability, achieving almost complete degradation of rifampicin and lincomycin within 6 minutes and 6 hours, respectively.

BIOCHEMICAL ENGINEERING JOURNAL (2023)

Article Chemistry, Applied

Eco-friendly marine antifouling coating consisting of cellulose nanocrystals with bioinspired micromorphology

Yanyi Duan, Jiangjiexing Wu, Wei Qi, Rongxin Su

Summary: A nanocomposite superhydrophobic coating was fabricated using natural sourced nanomaterials (cellulose nanocrystals, CNCs) as nanofillers via a simple sol-gel synthesis method. The coating with 20 wt% CNCs (CNC20) showed the best antifouling performance, reducing fouling by 82% compared to the control group in a 90-day marine field test. This biomimetic study provides a novel strategy for the development of environmentally friendly coatings with CNCs nanofillers.

CARBOHYDRATE POLYMERS (2023)

Article Biotechnology & Applied Microbiology

Rational design of tryptophan hydroxylation 1 for improving 5-Hydroxytryptophan production

Feifei Song, Tao Gu, Lin Zhang, Jiaxing Zhang, Shengping You, Wei Qi, Rongxin Su

Summary: This study improved the catalytic activity of L-Trp-TPH1-BH4 by rational design and obtained the S337A/F318Y beneficial mutant, which increased the yield of 5-HTP and produced 0.91 g/L of 5-HTP, offering the prospect of green production of 5-HTP.

ENZYME AND MICROBIAL TECHNOLOGY (2023)

Article Chemistry, Physical

Biospecific cation-p interaction by modulating molecular hydration and supramolecular structure of short peptides

Heng Chang, Vahid Adibnia, Rongxin Su, Wei Qi, Xavier Banquy

Summary: This study introduces new adhesive materials that use cation-p interactions for specific cohesive interaction in water. These materials are short peptides based on the FKF motif with different side groups. By controlling the composition of the side group, the strength and specificity of the cohesive and adhesive energies of the peptides can be finely tuned. The interfacial properties of the adhesive peptides depend on the deposition solvent, and using DMSO as the solvent results in higher cohesive and adhesive energies. The supramolecular structure of the peptide film, specifically needle-like structures, significantly enhance the adhesion of the material. Overall, cation-p interaction can be effectively utilized to create adhesive materials with features found in underwater adhesives, such as solvent displacement activation, as well as new properties like specificity and supramolecular structure enhanced adhesion.

JOURNAL OF COLLOID AND INTERFACE SCIENCE (2023)

Article Chemistry, Multidisciplinary

Peroxidase-Mimicking Hierarchically Organized Gold Particles for Glucose Detection

Xiaoxuan Ji, Qing Li, Rongxin Su, Yuefei Wang, Wei Qi

Summary: In this study, hierarchically organized gold nanoparticles (Au HOPs-X) with peroxidase-like catalytic activity were synthesized by reducing Au-thiolate hierarchically organized particles (Au HOPs) in situ. The Au HOPs-X exhibited enhanced catalytic activity compared to the initial Au HOPs due to the reduction of Au-thiolate complexes into Au nanoparticles and increased specific surface area. The highly catalytic Au HOPs-10 was combined with glucose oxidase to establish a standard curve for rapid detection of glucose through visual determination of solution saturation captured by mobile phone photos.

LANGMUIR (2023)

Article Green & Sustainable Science & Technology

Sustainable energy integration within the circular economy

Jiri Jaromir Klemes, Aoife Foley, Fengqi You, Kathleen Aviso, Rongxin Su, Awais Bokhari

Summary: This article highlights the importance of renewable and sustainable energy and circular economy in achieving sustainable development. It emphasizes the need for a portfolio of technologies and strategies to reduce the carbon emissions intensity of the energy sector and utilize renewable energy sources. The article also addresses the development of more sustainable and renewable energy devices and the establishment of infrastructure to improve energy efficiency. Lastly, it suggests the development of optimization models considering temporal and geographical constraints for the proper deployment of these strategies.

RENEWABLE & SUSTAINABLE ENERGY REVIEWS (2023)

Article Biotechnology & Applied Microbiology

Structural Properties of a Heteropolysaccharide Released from Isaria cicadae Miq. Solid-State Fermented Wheat Bran

Rui Liu, Ruixin Liu, Xuebing Yan, Ningjie Li, Ming Li, Zijian Zhi, Boli Guo, Min Zhang

Summary: The study aimed to improve the efficiency of wheat bran polysaccharide extraction through solid-state fermentation using the bioactive fungus Isaria cicadae Miq. The structural properties of the fermented wheat bran polysaccharide (IC-FWBP) were identified. The IC-FWBP consisted of mannose, glucose, and galactose, with glycosidic linkages and triple-helix conformations.

FERMENTATION-BASEL (2023)

Article Chemistry, Applied

Enzymatic oxidation of tyrosine enantiomers into biomimetic pigments with enhanced performance for hair dyeing

Yuhe Shen, Dongshuang Jia, Yuefei Wang, Tao Yu, Xiaojian Xu, Heng Chang, Qing Li, Rongxin Su, Wei Qi

Summary: In this study, we designed an asymmetric reaction system of tyrosine derivatives and demonstrated that the substrate molecular chirality can affect the reaction rate, intermolecular forces, and arrangement during assembly, thereby influencing the morphology, structure, and properties of biomimetic pigment materials. We also discovered that L + D-tyrosine derivatives can be oxidized and deposited on hair surfaces to achieve black and red-brown color effects, providing a green and safe biological hair dyeing method. Compared to previous studies, this approach offers faster coloring speed, higher color saturation, stronger color fixation stability, good mechanical strength, and biocompatibility, suggesting its potential for the manufacture of chiral-inspired advanced materials with bio-nanotechnology applications.

DYES AND PIGMENTS (2023)

Article Engineering, Chemical

The preparation of antifouling and superhydrophobic polyurethane sponge by grafting Econea to diisocyanate monomer for durable continuous oil/water separation and cleanup of oil spills

Haoyu Wang, Jinghui Wang, Mei Cui, Rongxin Su, Renliang Huang

Summary: A novel antifouling and superhydrophobic polyurethane sponge was successfully prepared, which showed excellent antifouling properties and super-hydrophobicity. It exhibited a high absorption capacity for trichloromethane and maintained a high absorption rate even after 50 cycles. It also demonstrated exceptional oil-water separation performance with high separation fluxes and efficiency. Therefore, this sponge is a promising and applicable material for long-term oil/water separation and cleanup of oil spills in marine environments.

SEPARATION AND PURIFICATION TECHNOLOGY (2023)

Article Chemistry, Applied

The first harmonised total diet study in Portugal: Vitamin D occurrence and intake assessment

M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira

Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

The digestion fates of lipids with different unsaturated levels in people with different age groups

Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu

Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Fabrication and characterization of chitosan-pectin emulsion-filled hydrogel prepared by cold-set gelation to improve bioaccessibility of lipophilic bioactive compounds

Hyunjong Yu, Huisu Kim, Pahn-Shick Chang

Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

The effect of lactic acid bacteria fermentation on physicochemical properties of starch from fermented proso millet flour

Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke

Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Novel competitive electrochemical impedance biosensor for the ultrasensitive detection of umami substances based on Pd/Cu-TCPP(Fe)

Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu

Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Identification and comparison of milk fat globule membrane and whey proteins from Selle Français, Welsh pony, and Tieling Draft horse mare's milk

Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang

Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Phenols and saliva effect on virgin olive oil aroma release: A chemical and sensory approach

Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales

Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Preparation and properties of pH-sensitive cationic starch nanoparticles

Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen

Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Direct seeding compromised the vitamin C content of baby vegetables and the glucosinolate content of mature vegetables in Asian leafy brassicas

Andrea Koo, Vinayak Ghate, Weibiao Zhou

Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

ACE inhibitory peptides from enzymatic hydrolysate of fermented black sesame seed: Random forest-based optimization, screening, and molecular docking analysis

Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie

Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Exploration of digestion-resistant immunodominant epitopes in shrimp (Penaeus vannamei) allergens

Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun

Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Effect of milling on in vitro Digestion-Induced release and bioaccessibility of active compounds in rice

Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan

Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Unraveling proteome changes of Sunit lamb meat in different feeding regimes and its relationship to flavor analyzed by TMT-labeled quantitative proteomic

Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin

Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Mechanism of aroma enhancement methods in accelerating Congou black tea acidification subjected to room temperature storage

Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni

Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.

FOOD CHEMISTRY (2024)

Article Chemistry, Applied

Immobilizing amyloglucosidase on inorganic hybrid nanoflowers to prepare time-temperature integrators for chilled pork quality monitoring

Lin Wang, Falai Ma, Zihan Li, Yan Zhang

Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.

FOOD CHEMISTRY (2024)