Article
Biochemistry & Molecular Biology
Rasa Garjonyte, Jurga Budiene, Linas Labanauskas, Asta Judzentiene
Summary: Leaves and stems of red raspberry have medicinal properties due to their high content of bioactive compounds, mainly polyphenols. Antioxidant activity of raspberry extracts is correlated with total phenolic content, while hydrogen peroxide-scavenging activity is related to the presence of proteins. The prooxidant activity of raspberry stem extracts is dependent on solution pH and temperature.
Article
Plant Sciences
Guoming Wang, Sophia Lee Guan, Nan Zhu, Qionghou Li, Xinran Chong, Tao Wang, Jiping Xuan
Summary: This study analyzed the characteristics, evolutionary history, and expression patterns of the SnRK gene family in black raspberry, and identified 209 SnRK genes that were classified into three subfamilies. Whole-genome duplication and dispersed duplication were major contributors to the expansion of the SnRK family. Purifying selection was the primary driving force in the evolution of the SnRK family. The spatial expression analysis revealed important roles of RoSnRK genes in different tissues, and 5 RoSnRK2 genes were found to respond to abiotic stresses.
Article
Horticulture
Pauliina Palonen, Tuomo Laine, Katriina Mouhu
Summary: The study evaluated the floricane cropping potential and berry quality in seven primocane raspberry cultivars, showing that growing them as long cane plants for summer crop production is more reliable than for fall cropping. Among the cultivars, 'Imara' and 'Kweli' were the most promising, with high yield potential and excellent berry quality.
SCIENTIA HORTICULTURAE
(2021)
Article
Agronomy
Dejan Zejak, Ivan Glisic, Velibor Spalevic, Pavle Maskovic, Branislav Dudic
Summary: The physical-geographical features of the mountainous area of Montenegro pose challenges to farmers. The study focused on the biological and production characteristics of two raspberry varieties grown in two locations. Results showed differences in growth and maturation time based on altitude.
Article
Plant Sciences
Fernanda Alvarez, Mario Moya, Claudia Rivera-Mora, Paz E. Zuniga, Karla Jara-Cornejo, Paula Munoz, Anibal Ayala-Raso, Sergi Munne-Bosch, Carlos R. Figueroa, Nicolas E. Figueroa, Monika Valdenegro, Juan E. Alvaro, Wilfried Schwab, Bruno G. Defilippi, Lida Fuentes
Summary: The study suggests that abscisic acid (ABA) signaling plays a role in the ripening of raspberry fruit, as evidenced by the concomitant increase in ABA content and the expression of ABA biosynthesis and receptor-related genes during fruit development.
Article
Horticulture
Lina M. Londono-Giraldo, Monica Bueno, Eduardo Corpas-Iguaran, Gonzalo Taborda-Ocampo, Alejandro Cifuentes
Summary: Carotenoids are naturally occurring antioxidants found in many organisms, with different cherry tomato varieties having varying carotenoid profiles and quantities. Wild red tomatoes have higher lycopene content than commercial tomatoes, while yellow tomatoes lack lycopene. Varieties with higher concentrations of carotenoids may have antioxidant activity. This study utilized HPLC-DAD-APCI-MS to analyze wild cherry tomato accessions, showing promising functional traits for potential commercial applications and local social projects.
Article
Plant Sciences
Xiaobai Li, Jian Sun, Zhen Chen, Jingyong Jiang, Aaron Jackson
Summary: This study investigated the carotenoid composition and biosynthetic pathways of Rubus chingii during fruit ripening. Zeaxanthin, beta-citraurin and their esters were found to gradually accumulate during maturation, responsible for fruit coloration. Differential gene/protein expressions in carotenoid metabolism and apocarotenoid biosynthesis led to zeaxanthin and beta-citraurin accumulation. Additionally, genes and enzymes were found to have distinct expression patterns, suggesting their specific functions and roles in fruit coloration.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Xiaobai Li, Jian Sun, Zhen Chen, Jingyong Jiang, Aaron Jackson
Summary: Chinese raspberry is a fruit native to China known for its health benefits and rich antioxidants. The study investigated the changes in carotenoids, phenolics, and antioxidant activity during different ripening phases. It found that while total phenolics decreased during fruit maturation, total carotenoids increased, leading to changes in antioxidant activity.
Article
Horticulture
Biljana B. Sikiric, Olivera S. Stajkovic-Srbinovic, Elmira R. Saljnikov, Andrey Litvinovich, Marina Jovkovic, Vesna V. Mrvic
Summary: The study evaluated the effects of lime and dolomite application on micronutrient concentrations in raspberry leaves and fruits grown in strongly acid soil. The results showed that lime reduced micronutrient content in the soil and plant, but additional doses may be required due to high concentrations of certain elements.
INTERNATIONAL JOURNAL OF FRUIT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Sylwia Cyboran-Mikolajczyk, Katarzyna Meczarska, Katarzyna Solarska-Sciuk, Katarzyna Ratajczak-Wielgomas, Jan Oszmianski, Vera Jencova, Dorota Bonarska-Kujawa
Summary: The aim of this study was to determine the biological activity of ellagitannins rich extracts from raspberry leaves and wild strawberry in relation to cells and cell membranes. The results showed that these extracts have protective effects on cells and membranes, and exhibit high biological activity, which may be used to delay aging and prevent diseases associated with oxidative stress.
Article
Food Science & Technology
Justyna Bauza-Kaszewska, Ewa Zary-Sikorska, Andrzej Gugolek, Anna Ligocka, Monika Kosmala, Elzbieta Karlinska, Bartosz Fotschki, Jerzy Juskiewicz
Summary: The antimicrobial activity of raspberry preparations on pathogenic bacteria was found to be weak, while probiotic Lactobacillus showed significant reduction in enteric pathogens count. The combined use of LAB and raspberry preparations resulted in total elimination of Salmonella strains and reduction in L. monocytogenes number.
POLISH JOURNAL OF FOOD AND NUTRITION SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Rasmus Bandick, Lia V. Busmann, Soraya Mousavi, Nizar W. Shayya, Jakub P. Piwowarski, Sebastian Granica, Matthias F. Melzig, Stefan Bereswill, Markus M. Heimesaat
Summary: This study investigated the disease-alleviating effects of menthol and various plant extracts in acute campylobacteriosis. The combination of these compounds alleviated diarrhea symptoms and reduced colonic T cell numbers in infected mice. However, it did not affect gastrointestinal pathogen loads or extra-intestinal immune responses.
Article
Multidisciplinary Sciences
Nathalie Kuhn, Jonathan Maldonado, Claudio Ponce, Macarena Arellano, Alson Time, Salvatore Multari, Stefan Martens, Esther Carrera, Jose Manuel Donoso, Boris Sagredo, Lee A. Meisel
Summary: Gibberellin (GA) has contrasting effects on two different sweet cherry varieties during fruit ripening, leading to differential modulation of gene expression and resulting in distinct characteristics at maturity.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Mareike Busche, Christopher Acatay, Stefan Martens, Bernd Weisshaar, Ralf Stracke
Summary: Bananas, well known for their edible fruits, are important perennial plants that contribute to food security and employment opportunities. Studies have shown that banana fruits contain high levels of minerals and phytochemicals, such as flavonoids, which are beneficial for human nutrition. Research on the enzymatic functionalities in bananas has provided insights for further genetic engineering to enhance the antioxidant activity and nutritional value of banana fruits.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Matti Mauramo, Tuulia Onali, Wafa Wahbi, Jenni Vasara, Anniina Lampinen, Elina Mauramo, Anne Kivimaki, Stefan Martens, Hely Haggman, Meeri Sutinen, Tuula Salo
Summary: This study found that bilberry powder can inhibit the viability, proliferation, migration, and invasion of oral squamous cell carcinoma cells, with more pronounced effects at higher concentrations. In both in vitro and in vivo experiments, bilberry powder showed anti-tumor effects on OSCC cells.
Article
Veterinary Sciences
Ioanna Poulopoulou, Mark James Horgan, Bianka Siewert, Michaela Siller, Luisa Palmieri, Eftychia Martinidou, Stefan Martens, Pietro Fusani, Veronika Temml, Hermann Stuppner, Matthias Gauly
Summary: The present study aims to find efficient alternatives to synthetic anthelmintics among ethno-veterinary herbs. The effects of methanolic extracts (ME) of nine plant species on the embryonic development (ED) of Ascaridia galli eggs were assessed. The results showed that these plant species have promising results in inhibiting ED, contributing to the identification of alternative anthelmintic treatments.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Plant Sciences
Giulia Pasqualetto, Luisa Palmieri, Stefan Martens, Vincent G. M. Bus, David Chagne, Claudia Wiedow, Mickael A. Malnoy, Susan E. Gardiner
Summary: Apple and pear are economically important fruit crops known for their unique qualities. This research utilized comparative genomic approaches to identify genetic differences and metabolite patterns, validating the effectiveness of three marker types in detecting hybridity between apple and pear. The findings will enhance breeding of new tree fruit crops, benefiting producers and consumers through marker assisted selection of desired traits between pear and apple.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Simon Miranda, Stefano Piazza, Floriana Nuzzo, Mingai Li, Jorge Lagreze, Axel Mithoefer, Alessandro Cestaro, Danuse Tarkowska, Richard Espley, Andrew Dare, Mickael Malnoy, Stefan Martens
Summary: The effects of targeting the key gene MdPGT1 involved in phloridzin biosynthesis in apple using conventional transgenesis and CRISPR/Cas9-mediated genome editing were simultaneously assessed. Knockdown lines showed impaired growth and leaf morphology, while genome-edited lines exhibited normal growth despite reduced phloridzin levels. Transcriptomic and metabolic analyses revealed regulated genes involved in phenylpropanoid and flavonoid pathways. Phytohormone profiling demonstrated differential modulation, with increased salicylic and jasmonic acid levels in dwarf lines and no correlation between auxin and ABA with growth phenotype.
Article
Plant Sciences
Simon Miranda, Jorge Lagreze, Anne-Sophie Knoll, Andrea Angeli, Richard Espley, Andrew P. Dare, Mickael Malnoy, Stefan Martens
Summary: In this study, researchers identified putative 3-hydroxylases in two wild Malus species that are capable of producing sieboldin, a specialised secondary metabolite of the dihydrochalcone group. They also discovered key residues mutations in the CYP98A protein of wild accessions that allow it to accept the substrate phloretin, leading to sieboldin accumulation. These findings provide important insights into the biosynthesis of sieboldin in Malus species.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Green & Sustainable Science & Technology
Virginia Sarropoulou, Eirini Sarrou, Andrea Angeli, Stefan Martens, Eleni Maloupa, Katerina Grigoriadou
Summary: Primula veris subsp. veris is a perennial herbaceous plant whose roots and flowers are valuable pharmaceutical raw materials. An in vitro adventitious root production method was developed to produce bioactive compounds in a controlled environment, leading to higher content compared to soil-grown plants.
Article
Plant Sciences
Eirini Sarrou, Lemonia Doukidou, Evangelia V. Avramidou, Stefan Martens, Andrea Angeli, Rafaela Stagiopoulou, Nikolaos M. Fyllas, Nikos Tourvas, Eleni Abraham, Eleni Maloupa, Irini Nianiou-Obeidat, Ioannis Ganopoulos, Nikos Krigas
Summary: This study evaluated the genetic and phyto-chemical diversity of Sideritis euboea populations in three mountainous areas of Evia Island, Greece. The analysis revealed differences between populations in terms of genetics and polyphenolic metabolites. The findings suggest that each population should be considered as a distinct conservation unit.
JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS
(2022)
Article
Horticulture
Giulia Lauria, Ermes Lo Piccolo, Anna Davini, Monica Ruffini Castiglione, Ylenia Pieracci, Guido Flamini, Stefan Martens, Andrea Angeli, Costanza Ceccanti, Lucia Guidi, Elisa Pellegrini, Luca Incrocci, Marco Landi
Summary: LED light supplementation with narrowband green (G), blue (B), red (R), and polychromatic (W - R:G:B, 1:1:1) lights was used to "photomodulate" the performance of green-leafed ('Tigullio'; TI) and red-leafed ('Red Rubin'; RR) sweet basil plants. The results showed that monochromatic LED lights selectively modulated the metabolomics profile of both basil cultivars, increasing antioxidant content and decreasing toxic compounds.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Umberto Salvagnin, Katharina Unkel, Thorben Sprink, Paul Bundock, Robert Sevenier, Milica Bogdanovic, Sladana Todorovic, Katarina Cankar, Johanna Christina Hakkert, Elio Schijlen, Ronald Nieuwenhuis, Maria Hingsamer, Veronika Kulmer, Michael Kernitzkyi, Dirk Bosch, Stefan Martens, Mickael Malnoy
Summary: In this study, different delivery methods of CRISPR/Cas9 reagents were compared for their efficiency in genome editing of chicory, as well as the frequency of off-target mutations and their environmental and economic impacts. The study found that using RNPs as the delivery method resulted in high editing efficiency, no off-target mutations, and avoided the integration of unwanted plasmid DNA and the need for gene segregation.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Kalliopi Kadoglidou, Catherine Cook, Anastasia Boutsika, Eirini Sarrou, Ifigeneia Mellidou, Christina Aidonidou, Ioannis Grigoriadis, Andrea Angeli, Stefan Martens, Vasiliki Georgiadou, Theodoros Moysiadis, Parthenopi Ralli, Ioannis Mylonas, Nikolaos Tourvas, Michail Michailidis, Apostolos Kalivas, Eleni Maloupa, Ioannis Ganopoulos, Aliki Xanthopoulou
Summary: Greek landraces of dill have distinct morphological, genetic, and chemical traits compared to modern commercial cultivars. Landraces have desirable characteristics such as taller height, larger umbels, and denser foliage. Genetic divergence exists between landraces and cultivars, but some gene flow may have occurred. Greek landraces of dill have potential for breeding programs to develop improved cultivars.
Article
Horticulture
Persefoni Maletsika, Vasiliki Liava, Eirini Sarrou, Vaia Styliani Titeli, Elpida Nasiopoulou, Stefan Martens, Evangelos Karagiannis, Katerina Grigoriadou, Athanassios Molassiotis, George D. Nanos
Summary: This study investigated the effect of pre-harvest foliar calcium application on fruit quality, primary metabolite profile, antioxidant activity, and phenolic compounds of the 'Lemonato' peach. The results showed that the different calcium treatments had no significant effect on the fruit quality characteristics of this peach variety. The response of fruit quality, metabolites, and phenolic compounds to organic calcium and calcium-silicate treatments varied among different orchards, suggesting the involvement of genetic or environmental factors.
Article
Agricultural Engineering
Virginia Sarropoulou, Eirini Sarrou, Andrea Angeli, Stefan Martens, Eleni Maloupa, Katerina Grigoriadou
Summary: In vitro elicitation technology can enhance the production of important secondary metabolites in Primula veris, providing ecological and economic advantages. In this study, eight abiotic elicitors were tested, and the optimal elicitors (L-proline and jasmonic acid) and elicitation conditions (60 days and 30 days) were determined. By using these elicitors and conditions, the yield of roots and the production of bioactive compounds can be improved.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Plant Sciences
Orlando Acevedo, Claudio Ponce, Macarena Arellano, Salvatore Multari, Esther Carrera, Jose Manuel Donoso, Stefan Martens, Nathalie Kuhn, Lee A. Meisel
Summary: Fruit development and color evolution are influenced by signals, particularly abscisic acid (ABA). Mutations in MYB factor genes lead to color differences between dark-red and bicolored sweet cherry cultivars. This study examined the involvement of ABA in the coloring process and found that ABA biosynthetic gene expression decreased in the bicolored cultivar but increased in the dark-red cultivar as fruit ripened. ABA treatment influenced color development in the dark-red cultivar but had no effect on the bicolored cultivar.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
