Article
Food Science & Technology
Xueran Geng, Jiayu Lei, Tergun Bau, Dongdong Guo, Mingchang Chang, Cuiping Feng, Lijing Xu, Yanfen Cheng, Ningke Zuo, Junlong Meng
Summary: In this study, a novel acidic alpha-galactosidase was purified from Oudemansiella radicata and further immobilized to obtain iORG. The immobilized enzyme showed higher stability and better degradation effect on raffinose family oligosaccharides (RFOs) in soymilk, indicating its potential application in the food industry.
Article
Biochemistry & Molecular Biology
Bing Hua, Mengying Zhang, Jinji Zhang, Haibo Dai, Zhiping Zhang, Minmin Miao
Summary: In cucumber fruits, RFOs hydrolysis mainly occurs in the vascular tissues. CsAGA1 and CsAGA2 play key but partly distinct roles in the hydrolysis of RFOs, with three alkaline forms of alpha-Gals being preferentially expressed in fruit vascular tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xixi Bai, Xiaoyang Sun, Yue Yu, Yuheng Guo, Linyu Nian, Chongjiang Cao, Shujie Cheng
Summary: In this study, a PVA-CS-GMA material was successfully synthesized and used to immobilize α-galactosidase. The resulting material had a stable porous network and showed suitable mechanical properties and high water content for retaining catalytic activity. The immobilized α-galactosidase exhibited improved enzymatic properties and reusability compared to the free enzyme. It was successfully applied in the hydrolysis of RFOs in soybeans, providing a new strategy for diet intervention in IBS.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Multidisciplinary
Yumin Zhang, Dan Li, Lynnette M. A. Dirk, A. Bruce Downie, Tianyong Zhao
Summary: ZmAGA1 overexpression in Arabidopsis reduces RFOs and galactinol content in seeds, leading to increased seed germination percentage under stress conditions, but decreased seed aging tolerance. Manipulation of ZmAGA1 expression can potentially regulate maize seed germination and aging tolerance simultaneously.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Evran Bicak Celem, Secil Onal
Summary: The immobilized alpha-galactosidase can effectively hydrolyze RFOs in soymilk, thus improving its nutritional value.
PROCESS BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Lei Li, Carmela Belloch, Monica Flores
Summary: This study investigated the impact of replacing pork lard with coconut oil and inoculating Debaryomyces hansenii on the transformation of amino acids into volatile compounds in a meat model system. The research used yeast counts, solid-phase microextraction, and gas chromatography/mass spectrometry to measure yeast growth and volatile production. The results showed that the presence of fat and yeast influenced the composition of volatile compounds, and different effects were observed with pork lard and coconut oil models. Yeast activity also affected the degradation of amino acids and the generation of specific compounds.
Article
Horticulture
Surelys Ramos-Bell, Luis G. Hernandez-Montiel, Rita M. Velazquez-Estrada, Juan A. Herrera-Gonzalez, Porfirio Gutierrez-Martinez
Summary: The study found that antagonistic yeast Debaryomyces hansenii from marine origin can effectively inhibit the growth of Botrytis cinerea in blueberries, reduce disease incidence, and prolong the shelf life of the fruits.
Article
Environmental Sciences
Jasvinder Kaur, Vandana Anand, Sonal Srivastava, Vidisha Bist, Mariya Naseem, Pallavi Singh, Vartika Gupta, Poonam C. Singh, Sangeeta Saxena, Saraswati Bisht, Pankaj Kumar Srivastava, Suchi Srivastava
Summary: The study aimed to investigate the use of microbial co-inoculants for mitigating arsenic (As) toxicity in rice. The combination of a yeast strain (Debaryomyces hansenii NBRI-Sh2.11) and a bacterial strain (Citrobacter sp. NBRI-B5.12) was found to significantly reduce the accumulation of As in rice grain under stressed conditions. This reduction in As accumulation was associated with enhanced nutrient uptake and increased production of indole acetic acid (IAA) and gibberellic acid (GA) in the plants. The results suggest that these microbial co-inoculants have the potential to alleviate As toxicity and improve biomass in rice.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biotechnology & Applied Microbiology
Carmela Belloch, Laura Perea-Sanz, Amparo Gamero, Monica Flores
Summary: This study evaluated 60 strains of D. hansenii for their potential application as starters in dry-cured fermented sausages. The results showed that D. hansenii is a safe yeast, capable of tolerating abiotic stress and generating desirable aromas. The selected strains were deemed suitable for use as starters in meat products.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Plant Sciences
Udhaya Kannan, Roopam Sharma, Manu P. Gangola, Seedhabadee Ganeshan, Monica Baga, Ravindra N. Chibbar
Summary: This study investigated the accumulation mechanism of raffinose family oligosaccharides during lentil seed development, confirming the important roles of RS and STS in the accumulation process, and examining the trends of various oligosaccharides at different stages of development.
JOURNAL OF PLANT PHYSIOLOGY
(2021)
Article
Food Science & Technology
Xymena Polomska, Cecile Neuveglise, Joanna Zyzak, Barbara Zarowska, Serge Casaregola, Zbigniew Lazar
Summary: This study reveals a higher prevalence of VLEs in freshly isolated D. hansenii killer yeast compared to strains stored in culture collections. The newly identified VLE systems show high conservation, but do not participate in the biocontrol of other fungi present in blue cheeses.
Article
Biotechnology & Applied Microbiology
Martina Aulitto, Andrea Strazzulli, Ferdinando Sansone, Flora Cozzolino, Maria Monti, Marco Moracci, Gabriella Fiorentino, Danila Limauro, Simonetta Bartolucci, Patrizia Contursi
Summary: This study demonstrates the ability of B. coagulans MA-13 to over-produce α-galactosidase and β-galactosidase, which are of great importance to the food industry. This paves the way for potential use in treating gastrointestinal diseases.
MICROBIAL CELL FACTORIES
(2021)
Article
Chemistry, Applied
Esin Calci, Secil Onal
Summary: This study presents a new strategy for enzyme immobilization, called bioaffinity immobilization, which utilizes biospecific affinity interactions between enzymes and matrices. The authors immobilized alpha-galactosidase on chitosan functionalized with Concanavalin A using three different methods. The results showed that each method had different immobilization yields and activity levels, with method II achieving the highest immobilization yield and method III exhibiting the greatest hydrolysis efficiency for raffinose. The immobilized enzymes also demonstrated promising biochemical characterization and stability, indicating potential applications in the food and feed industries.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Agriculture, Dairy & Animal Science
Xingbo Liu, Kun Xing, Ran Ning, Sergi Carne, Xingqiang Wu, Wei Nie
Summary: The experiments showed that enzyme supplementation improved the digestibility of calcium and ileal digestibility of total amino acids in broiler chickens. Additionally, enzyme inclusion increased the digestibility of crude protein, gross energy, raffinose, and stachyose, improved average daily gain, and reduced chyme viscosity in the jejunum. These results support the positive effects of enzyme supplementation on nutrients digestibility and growth performance in broiler chickens.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Weizhe Wang, Sudun, Huizhong Hu, Jieran An, Hongxing Zhang, Zigang Zhao, Yanling Hao, Zhengyuan Zhai
Summary: This study investigates the impact of raffinose on the growth of L. salivarius and identifies the genes responsible for raffinose transport and hydrolysis. Results show that the RafP gene plays an important role in raffinose transport, while GalA1 is the primary enzyme involved in raffinose hydrolysis.
Article
Biochemistry & Molecular Biology
Roberta Corsino Ferreira, Murillo Peterlini Tavares, Tulio Morgan, Yan da Silva Clevelares, Marina Quadri Raposo Branco Rodrigu, Maria Catarina Megumi Kasuya, Tiago Antonio de Oliveira Mendes, Valeria Monteze Guimaraes
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Bruna Cristina dos Santos Cruz, Vinicius da Silva Duarte, Alessio Giacomini, Viviana Corich, Sergio Oliveira de Paula, Lilian da Silva Fialho, Valeria Monteze Guimaraes, Celia Lucia de Luces Fortes Ferreira, Maria do Carmo Gouveia Peluzio
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Energy & Fuels
Mariana Furtado Granato de Albuquerque, Valeria Monteze Guimaraes, Sebastiao Tavares de Rezende
Summary: The study evaluated the impact of different lignocellulosic materials on the enzyme production of Chrysoporthe cubensis, and found that a new enzyme cocktail derived from the combination of wheat bran and sugar beet flour showed the highest efficiency in biomass hydrolysis.
BIOENERGY RESEARCH
(2021)
Article
Agricultural Engineering
Marcele Pandelo Martins, Rafaela Ines de Souza Ladeira Azar, Gabriela Piccolo Maitan-Alfenas, Valeria Monteze Guimara
BIOMASS & BIOENERGY
(2020)
Article
Biochemical Research Methods
Murillo Peterlini Tavares, Tulio Morgan, Riziane Ferreira Gomes, Marina Quadrio Raposo Branco Rodrigues, William Castro-Borges, Sebastiao Tavares de Rezende, Tiago Antonio de Oliveira Mendes, Valeria Monteze Guimaraes
Summary: The phytopathogenic fungus Chrysoporthe cubensis has a high capacity to produce efficient enzymes for lignocellulosic biomass hydrolysis. The study identified a diverse set of enzymes in the fungal extract, indicating versatility in acting on complex biomasses. This research provides insights into lignocellulose degradation mechanisms and the potential industrial applications of C. cubensis enzymes.
JOURNAL OF PROTEOMICS
(2021)
Article
Biotechnology & Applied Microbiology
Marciana da Luz Morales, Lucas Filipe Almeida, Rafaela Ines de Souza Ladeira Azar, Valeria Monteze Guimaraes, Rafael Ferreira Alfenas, Gabriela Piccolo Maitan-Alfenas
Summary: The study revealed the biotechnological potential of Kretzschmaria zonata for producing various enzymes, particularly xylanase, after growth on corn cob. The fungus extract showed synergy with commercial enzyme mixtures, indicating its potential for second generation ethanol production.
Article
Chemistry, Analytical
Richard Marins da Silva, Valeria Monteze Guimaraes, Lizzy Ayra Alcantara Verissimo, Marcia Cristina Teixeira Ribeiro Vidigal, Valeria Paula Rodrigues Minim, Luis Antonio Minim
Summary: This study successfully extracted and purified gamma-conglutin, a protein from lupin seeds known for its blood glucose lowering properties. The purification process was effective in obtaining a high degree of purity, and the protocol used was simple and easily scalable.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Tulio Morgan, Daniel Luciano Falkoski, Murillo Peterlini Tavares, Mariana Bicalho Oliveira, Valeria Monteze Guimaraes, Tiago Antonio de Oliveira Mendes
Summary: Filamentous fungi are prolific producers of carbohydrate-active enzymes and play an important role in plant cell wall degradation. In this study, the ability of selected fungi to produce plant cell wall-degrading enzyme activities was evaluated, and the culture supernatant of one fungus showed promising effects on enhancing sugarcane straw saccharification. Genome and proteomic analyses revealed a high repertoire of cell wall-degrading enzyme-coding genes in this fungus, which likely contribute to improved saccharification performance.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tulio Morgan, Murillo Peterlini Tavares, Rafaela I. S. Ladeira-Azar, Tiago Antonio de Oliveira Mendes, Valeria Monteze Guimaraes
Summary: This study investigated the impact of oxidoreductases secreted by Chrysoporthe cubensis on the saccharification of sugarcane bagasse. Genome and proteomic analyses revealed different functional and evolutionary features of lytic polysaccharide monooxygenases (LPMO9s) in C. cubensis. The study also identified clusters enriched with phylo-specific LPMO9 and clusters containing LPMO9s from fungi with no genes coding for cellobiose dehydrogenase (CDH). Furthermore, the experiment showed that CcLPMO9C and CcLPMO9D were better suited for sugarcane bagasse saccharification compared to other LPMO9s secreted by C. cubensis.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Marciana da Luz Morales, Rafaela Ladeira de Souza Azar, Valeria Monteze Guimaraes, Rafael Ferreira Alfenas, Gabriela Piccolo Maitan-Alfenas
Summary: This study discovered that the xylanase produced by the fungus Kretzschmaria zonata after growth on corncob can efficiently hydrolyze beechwood xylan, resulting in xylobiose and xylotriose, which have the potential to be used as prebiotics.
JOURNAL OF FOOD PROCESSING AND PRESERVATION
(2022)
Article
Mycology
Tulio Morgan, Fabio Alex Custodio, Murillo Peterlini Tavares, Olinto Liparini Pereira, Valeria Monteze Guimaraes, Tiago Antonio de Oliveira Mendes
Summary: This study reports a new endophytic Trichoderma species isolated from orchid roots, which has undergone genome simplification and exhibits the ability to degrade plant cell wall.
MYCOLOGICAL PROGRESS
(2022)
Article
Biochemistry & Molecular Biology
Murillo Peterlini Tavares, Thiago Rodrigues Dutra, Tulio Morgan, Rafaela Zandonade Ventorim, Rafaela Ines de Souza Ladeira Azar, Ednilson Mascarenhas Varela, Roberta Corsino Ferreira, Tiago Antonio de Oliveira Mendes, Sebastiao Taveres de Rezende, Valeria Monteze Guimaraes
Summary: The genome of the phytopathogenic fungus Chrysoporthe cubensis contains distinct groups of multicopper oxidase (MCO) coding genes, including laccases, which have potential for biotechnological applications. In this study, the complete genomic arsenal of MCOs from C. cubensis was identified and characterized. Laccases were induced, purified, and tested for improving sugarcane bagasse saccharification. The fungal cultivation in different lignocellulosic biomass yielded more efficient enzymatic extracts than commercial preparations, with the highest laccase activity detected in the crude extract of the fungus cultivated on wheat bran and orange peel (3:1).
PROCESS BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Cristina P. S. Martins, Denise Fernandes, Valeria M. Guimaraes, Dongliang Du, Delmira C. Silva, Alex-Alan F. Almeida, Frederick G. Gmitter, Wagner C. Otoni, Marcio G. C. Costa
Summary: This study assessed the potential functions of citrus galactinol synthase (GolS) in stress response and tolerance mechanisms. The results showed that citrus GolS genes have low-level tissue-specific and stress-inducible expression in response to drought, salt stress, and infection. Overexpression of CsGolS6 improved tobacco tolerance to drought and salt stresses, leading to increased mesophyll cell expansion, photosynthesis, and plant growth. Metabolite profiling revealed no significant changes in endogenous galactinol, but an increase in metabolites with antioxidant properties was observed in the transgenic plants.
Article
Engineering, Chemical
Thiago Rodrigues Dutra, Valeria Monteze Guimaraes, Ednilson Mascarenhas Varela, Mariana Furtado Granato de Albuquerque, Juan Diego Rios Diez, Acelino Couto Alfenas, Sebastiao Tavares de Rezende
Summary: An enzyme cocktail (SSF-SmF-cocktail) was produced by extracting enzymes of Chrysoporthe cubensis grown under solid-state fermentation and submerged fermentation. This cocktail showed the highest efficiency in glucose release during alkali-pretreated sugarcane bagasse hydrolysis, indicating its potential for industrial applications.
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Biology
Mariana Furtado Granato de Albuquerque, Tiago Ferreira Leal, Rafaela Ines de Souza Ladeira Azar, Adriane Maria Ferreira Milagres, Valeria Monteze Guimaraes, Sebastiao Tavares de Rezende
Summary: This study demonstrates that the enzymatic cocktails produced by Chrysoporthe cubensis and Penicillium pinophilum fungi have efficient hydrolytic activity on biomass pretreated with hot water, leading to significant glucose and xylose production. The use of hydrothermal pretreatment without chemical compounds enhances the performance of the enzymes, contributing to energy savings in the ethanol production process.
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2021)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
