Article
Biochemistry & Molecular Biology
Isabel Fraile-Gutierrez, Susana Iglesias, Niuris Acosta, Julia Revuelta
Summary: This research developed a new hydrogel that can protect beta-galactosidase from degradation in the stomach and retain its activity in the intestine. It represents a promising approach for oral transport of the enzyme to reduce the symptoms of hypolactasia.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Leszek Kadzinski, Robert Lyzen, Katarzyna Bury, Bogdan Banecki
Summary: The studies demonstrate that by optimizing the sol-gel process parameters and adding polydimethylsiloxane, the activity and thermal stability of beta-galactosidase can be enhanced, which is of great significance for industrial applications such as lactose elimination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Zhenshang Xu, Can Li, Yanxin Ye, Ting Wang, Susu Zhang, Xinli Liu
Summary: Transcriptome sequencing revealed the gene expression difference of Lactiplantibacillus plantarum in glucose and lactose medium, identifying that beta-galactosidase LacLM plays a major role in lactose utilization, providing a theoretical foundation for the growth and metabolism of Lp. plantarum in lactose medium.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Polymer Science
J. Lucas Vallejo-Garcia, Ana Arnaiz, Maria D. Busto, Jose M. Garcia, Safil Vallejos
Summary: This study presents the synthesis and characterization of a polyacrylic film material for enzyme immobilization. The material achieves efficient immobilization of the enzyme through the formation of azo linkages and demonstrates superior performance in lactose hydrolysis.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Agricultural Engineering
Kevin Munoz, Diego Ahumada, Felipe Arenas, Cecilia Guerrero, Andres Illanes, Carlos Vera
Summary: The study found that butyl-beta-galactoside is significantly more soluble in the organic phase than lactose and monosaccharides. Additionally, higher values of the 1-butanol/aqueous phase ratio were shown to reduce secondary hydrolysis and increase yields. Interfacial properties for butyl-beta-galactoside were also determined at 25 degrees C.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Physical
Sami A. Al-Harbi, Yaaser Q. Almulaiky
Summary: The immobilization of beta-galactosidase on Cu-trimesic acid-based metal organic frameworks (Cu-TMAs) was studied. The immobilized enzyme showed good retention of activity, storage stability, and improved lactose hydrolysis activity. This study demonstrated the potential of Cu-TMAs as supports for immobilizing beta-galactosidase and suggested practical applications in various industrial processes.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Microbiology
Kalathinathan K. Pooja, Sankaranarayanan Gomathinayagam, Kodiveri M. Gothandam
Summary: The genome of Paracoccus marcusii KGP, isolated from marine sediment collected from the coast of the Bay of Bengal, was sequenced using Oxford Nanopore sequencing technology. The genome contains genes for industrially relevant enzymes and secondary metabolites, with the beta-galactosidase showing transgalactosylation property. Comparison with the CGMCC strain revealed significant differences in the KGP genome, possibly indicating strain-specific genes.
CURRENT MICROBIOLOGY
(2021)
Review
Food Science & Technology
Patrick Schulz, Syed S. H. Rizvi
Summary: The lactose-free dairy market has grown significantly in recent years, with lactose hydrolysis being the most commonly used method due to its economic and sustainable advantages. Current research mainly focuses on enzymatic hydrolysis methods and developing new commercially valuable products.
FOOD REVIEWS INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Yuchen Li, Svitlana Miros, Hossein Kiani, Hans-Georg Eckhardt, Alfonso Blanco, Shane Mulcahy, Hugh McDonnell, Brijesh Kumar Tiwari, Ronald Halim
Summary: This study investigated the mechanism of lactose assimilation in Nannochloropsis oceanica for dairy-wastewater bioremediation and co-production of valuable feed/food ingredients. It was found that mixotrophic cultivation is necessary for lactose assimilation in N. oceanica, and the microalgae can produce beta-galactosidase enzyme to break down lactose. Under mixotrophic conditions, the microalgae can maintain dominance in the culture, while under heterotrophic conditions, bacteria take over the culture. These results have implications for onsite dairy wastewater treatment.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Chemistry, Analytical
Xiaobin Zhou, Mengjun Wang, Junyang Chen, Xingguang Su
Summary: In this study, a high-performance nanozyme, Cu/NC NS, was designed and synthesized for the construction of a high-sensitivity biosensor platform. The Cu/NC NS exhibited high affinity towards H2O2 and TMB under acidic condition, allowing for the accurate detection of lactose and β-Gal through a multienzyme cascade colorimetric sensing system.
Article
Agriculture, Multidisciplinary
Belay Tilahun Tadesse, Ge Zhao, Paul Kempen, Christian Solem
Summary: This study discovered that a mutated strain of Streptococcus thermophilus can efficiently hydrolyze lactose using whole-cell lactase catalysts. Genome sequencing revealed a mutation that affects the growth of the strain and increases its lactose hydrolyzing ability. These findings could potentially lead to the production of sweet lactose-reduced yoghurt without the need for expensive purified lactase enzymes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Shuyue Luan, Xuguo Duan
Summary: In this study, a novel thermostable β-galactosidase (lacZBa) from Bacillus aryabhattai GEL-09 was investigated and found to have potential applications in the dairy industry. LacZBa exhibited maximum activity at 45°C and pH 6.0, and could hydrolyze lactose and oNPG. It efficiently recognized and catalyzed lactose, and could completely hydrolyze lactose in lactose solution and commercial milk within 4 hours at 45°C.
Article
Food Science & Technology
T. Inanan
Summary: This study aimed to optimize lactase immobilization onto Fe-chelated cryogel disks, and found that the immobilized lactase showed higher catalytic activity at different pH and temperature conditions, making it suitable for lactose-free milk preparation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jingjing Sun, Congyu Yao, Yujie Li, Wei Wang, Jianhua Hao, Yi Yu
Summary: In this study, a novel β-galactosidase was isolated from a deep-sea metagenome, overexpressed in Escherichia coli, and purified. The enzyme showed high activity at specific temperatures and pH conditions, and exhibited resistance to salt. Moreover, it displayed hydrolysis and transglycosylation activity towards substrates such as oNPG and lactose.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
David Orrego, Bernadette Klotz-Ceberio
Summary: Valorization of by-products in the dairy industry is a priority worldwide to promote circular economies. This study demonstrates a process to produce galacto-oligosaccharides (GOS) from whey permeate, which can be used directly in daily dairy products. The results show high GOS yield and utilization, and improved flavor and mouthfeel in the final product.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Multidisciplinary
Hee Uk Lee, Joon-Hyung Jin, Seung Wook Kim
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Laxmi Prasad Thapa, Sang Jun Lee, Chulhwan Park, Seung Wook Kim
BIOCHEMICAL ENGINEERING JOURNAL
(2019)
Article
Agricultural Engineering
Sun-A Choi, You-Kwan Oh, Jiye Lee, Sang Jun Sim, Min Eui Hong, Ji-Yeon Park, Min-Sik Kim, Seung Wook Kim, Jin-Suk Lee
BIORESOURCE TECHNOLOGY
(2019)
Article
Chemistry, Physical
Youngsang Chun, Young Gun Ko, Taegu Do, Youngkyun Jung, Seung Wook Kim, Ung Su Choi
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2019)
Article
Chemistry, Multidisciplinary
Dong Sup Kim, Han Suk Choi, Xiaoguang Yang, Ji Hyun Yang, Ja Hyun Lee, Hah Young Yoo, Jinyoung Lee, Chulhwan Park, Seung Wook Kim
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Soo Kweon Lee, Ju Hun Lee, Hyeong Ryeol Kim, Youngsang Chun, Ja Hyun Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim
Article
Chemistry, Physical
Ji Hyun Yang, Hyeong Ryeol Kim, Ja Hyun Lee, Joon-Hyung Jin, Hee Uk Lee, Seung Wook Kim
Summary: The study aimed to enhance power output by optimizing electrical communication for GOx on a composite surface and found that immobilized GOx at a concentration of 40:80 mM EDC:NHS exhibited the highest activity with a power density of 2.24 mWcm(-2) under optimal conditions.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Green & Sustainable Science & Technology
Hyeong Ryeol Kim, Ju Hun Lee, Soo Kweon Lee, Youngsang Chun, Chulhwan Park, Joon-Hyung Jin, Hee Uk Lee, Seung Wook Kim
Summary: The study demonstrates the fabrication of a flexible MnO2/HNO3 pretreated biochar (NBC)/Poly(3,4-ethylenedioxythiophene) (PEDOT) composite microsupercapacitor (MSC) using pen lithography. The HNO3 pretreatment improves the dispersion of biochar ink and increases its specific surface area and graphitic carbon structure, resulting in high capacitance.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Environmental Sciences
Kang Hyun Lee, Soo Kweon Lee, Jeongho Lee, Seunghee Kim, Chulhwan Park, Seung Wook Kim, Hah Young Yoo
Summary: By pretreating chestnut shells with KOH, this study successfully increased their enzymatic digestibility and optimized reaction conditions, resulting in a significant increase in glucan content and enzymatic digestibility of untreated chestnut shells.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Engineering, Chemical
Ju Hun Lee, Do Yoon Lee, Soo Kweon Lee, Hyeong Ryeol Kim, Youngsang Chun, Hah Young Yoo, Ho Seok Kwak, Chulhwan Park, Ja Hyun Lee, Seung Wook Kim
Summary: This study successfully produced 2,3-butanediol by simultaneously utilizing sugars extracted from microalgae and crude glycerol. The production yield was increased and the medium composition was optimized, contributing to the development of zero-waste processes.
Article
Engineering, Environmental
Youngsang Chun, Kyung Rae Kim, Hyeong Ryeol Kim, Soo Kweon Lee, Ju Hun Lee, Ja Hyun Lee, Chulhwan Park, Hah Young Yoo, Seung Wook Kim
Summary: The study enhanced the mechanical strength of biosorbents by using a biochar-alginate composite with a melamine sponge, analyzed the Cu(II) adsorption capacity using suitable adsorption models, and determined the maximum adsorption capacity to be 126.58 mg/g. The biosorbent was found to be effective for removing Cu(II) from wastewater.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Environmental Sciences
Kang Hyun Lee, Soo Kweon Lee, Jeongho Lee, Seunghee Kim, Seung Wook Kim, Chulhwan Park, Hah Young Yoo
Summary: This study improved the biomass-to-glucose conversion process by optimizing the utilization of chestnut shell for bioethanol production. The energy consumption was reduced by designing an efficient pretreatment process. The ethanol yield reached 98%, suggesting the potential of chestnut shell as a feedstock for biorefinery.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Analytical
Dong Sup Kim, Xiaoguang Yang, Ja Hyun Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim, Jinyoung Lee
Summary: Electrochemical nano-biosensors are widely used in various industries for qualitative and quantitative analyses due to their high sensitivity. This study focused on finding stable and efficient materials for real-time detection. The composite of graphite oxide/cobalt/chitosan was found to have excellent stability and electron transfer capability, and was applied in a bio-electrochemical nano-biosensor. The developed sensor successfully detected D-glucose in real-time over a wide range of concentrations, and maintained stable detection response even after 14 days.
Article
Biotechnology & Applied Microbiology
Hyeong Ryeol Kim, Kang Hyun Lee, Youngsang Chun, Soo Kweon Lee, Ju Hun Lee, Seung Wook Kim, Hah Young Yoo
Summary: Food waste-based biorefineries are important for achieving a sustainable circular economy. This study utilized cheese whey powder to produce cyclosporin A and showed the potential of using cheese whey powder as a sustainable feedstock for biorefineries.
FERMENTATION-BASEL
(2022)
Article
Polymer Science
Hye-Jin Lee, Il-Ho Choi, Seung-Wook Kim, Kyung-Ran Hwang
Summary: Al2O3 prepared by solvothermal synthesis was used as a catalyst for the catalytic dehydration of a bio-based fatty alcohol, selectively producing long-chain linear-alpha-olefins (LAO). By adjusting the synthesis temperature, amorphous alumina with the highest surface area and total pore volume was obtained, achieving efficient dehydration results.
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.
