Article
Chemistry, Analytical
Qingqing He, Mian Wang, Yajie Zhao, Guiyu Tan, Man Zhang, Rui Feng, Yujie Chen, Baomin Wang, Qing X. Li
Summary: To improve the selection efficiency of nanobodies against atrazine in a phage display library, anti-carrier protein phages were depleted using immunomagnetic beads conjugated with bovine serum albumin. This depletion strategy tripled the number of atrazine-positive phage clones and resulted in the selection of a sensitive phage clone with greater thermostability and organic solvent tolerance compared to a monoclonal antibody. The Nb3-based immunoassay showed similar specificity but higher IC50 and method detection limit compared to the mAb 5D9-based immunoassay.
ANALYTICA CHIMICA ACTA
(2023)
Article
Environmental Sciences
Lianrun Huang, He Chen, Panpan Cui, Yuan Ding, Minghua Wang, Xiude Hua
Summary: In this study, haptens with carboxylic groups were synthesized and used to produce antibodies, leading to the development of a rapid and accurate assay for determining PND. The assay showed low detection limit, minimal cross-reactivity with PND analogs, and good correlation with traditional analysis methods.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Applied
Robert M. Hnasko, Alice V. Lin, Jeffery A. McGarvey, Christopher P. Mattison
Summary: This study developed sensitive and selective immunoassays for the detection of peanut allergen Ara h1, which can provide a rapid surveillance method for peanut in food production and consumption.
Article
Plant Sciences
Kaewta Rattanapisit, Tharita Kitisripanya, Atthaphon Konyanee, Worapol Sae-Foo, Apisit Burapapiruin, Waraporn Putalun, Seiichi Sakamoto, Waranyoo Phoolcharoen, Gorawit Yusakul
Summary: The plant expression platform is a new source for producing IgG targeting small low-molecular-weight molecules. The study demonstrated that plant-produced functional IgG maintained the binding activity and specificity of IgG derived from mammalian cells, showing potential as an efficient and cost-effective platform for antibody production in immunoassays targeting low-molecular-weight compounds.
PLANT CELL REPORTS
(2021)
Article
Chemistry, Analytical
Yi Li, Fei Deng, Ewa M. Goldys
Summary: The study demonstrates the successful integration of CRISPR/Cas12a-based signal amplification into immunoassays using a functional hybrid conjugate of antibody and DNA oligonucleotide. This approach improves the sensitivity of conventional immunoassays, allowing for the detection of small proteins with attomolar sensitivity. The CRISPR-based Universal Immunoassay Signal Enhancer (CRUISE) can be applied to various immunoassays and has the potential to enhance sensitivity and expand the detection range.
Review
Biotechnology & Applied Microbiology
Clare Mills, Katrina Campbell
Summary: There is an increasing demand for rapid and affordable in-field screening methods for low molecular weight (LMW) compound detection. This review examines the recent resurgence of using anti-idiotypes (Ab2s) and the potential role of phage display and nanobodies in improving LMW compound immunoassays.
TRENDS IN BIOTECHNOLOGY
(2022)
Article
Microbiology
Francisca J. Grill, Collin Jugler, Erin Kaleta, Qiang Chen, D. Mitchell Magee, Thomas E. Grys, Douglas F. Lake, Agostinho Carvalho, Joshua Fierer
Summary: At least half a million diagnostic tests for coccidioidomycosis are conducted annually in the southwestern and western regions of the United States. These tests require precise dilution of patient serum prior to testing. However, the FDA-approved enzyme immunoassays (EIAs) used for coccidioidomycosis diagnosis do not provide controls for the dilution step. This study evaluates the use of a humanized monoclonal antibody as a dilution control in these EIAs and demonstrates its effectiveness in ensuring accurate dilution and reliable results for diagnosis of coccidioidomycosis.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Jean N. Manirarora, Kristen E. Walker, Veerupaxagouda Patil, Gourapura J. Renukaradhya, Joanna LaBresh, Yvonne Sullivan, Ore Francis, Joan K. Lunney
Summary: This study aimed to generate priority immune reagents for pigs and market them. Soluble swine cytokines and monoclonal antibodies (mAbs) were successfully produced. These new antibodies have expanded opportunities for immunology research in swine.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Jinxin He, Yan Zheng, Xiaorong Chen, Na Li, Fang Tang, Nairui Huo, Jianzhong Wang, Shaopeng Gu
Summary: A rabbit IgG-based ELISA was developed for the sensitive and specific detection of MBZ residues in food samples, showing promise for routine screening analysis.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Yuchen Bai, Jie Fei, Weilin Wu, Leina Dou, Minggang Liu, Shibei Shao, Wenbo Yu, Kai Wen, Jianzhong Shen, Zhanhui Wang
Summary: This study determined the minimum distance between two epitopes in sandwich immunoassays for small molecules. The results provide a theoretical basis for hapten design, antibody discovery and development, and the establishment of sandwich immunoassays.
ANALYTICAL CHEMISTRY
(2022)
Review
Environmental Sciences
Xiang Cheng, Ting Luo, Feiyi Chu, Bin Feng, Shibo Zhong, Fei Chen, Jie Dong, Wenbin Zeng
Summary: This review summarizes the recent advances in multifunctional materials for detection, detoxification, adsorption, and removal of mercury ions, discusses the advantages and disadvantages of different types of sensors, and points out the future development direction.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Georg Semmler, Marianna Theresia Traugott, Marianne Graninger, Wolfgang Hoepler, Tamara Seitz, Hasan Kelani, Mario Karolyi, Erich Pawelka, Sara Aragon de La Cruz, Elisabeth Puchhammer-Stockl, Stephan Walter Aberle, Karin Stiasny, Alexander Zoufaly, Judith Helene Aberle, Lukas Weseslindtner
Summary: This study found that differences in sensitivity among commercial immunoassays during acute SARS-CoV-2 infection are only partly related to the target antigen. NCP-specific IgA and IgG antibodies are detected earlier, while higher levels of S1-specific IgA antibodies occur in severely ill patients.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Chemistry, Applied
Supaluk Krittanai, Piriyakorn Pichetpongtorn, Seiichi Sakamoto, Waraporn Putalun
Summary: Licochalcone A (LicoA), a compound found in Chinese licorice, has been approved for the treatment of rosacea and acne due to its antimicrobial and anti-inflammatory properties. A monoclonal antibody against LicoA was developed using a mannich reaction hapten conjugate and hybridoma technique. An ELISA method was then developed to quantitatively determine the concentration of LicoA. This method showed high specificity and can be used for quality control of licorice and its derived products.
FOOD AND AGRICULTURAL IMMUNOLOGY
(2022)
Article
Virology
Yassein M. Ibrahim, Wenli Zhang, Gebremeskel Mamu Werid, He Zhang, Yawen Feng, Yu Pan, Lin Zhang, Changwen Li, Huan Lin, Hongyan Chen, Yue Wang
Summary: Porcine sapelovirus (PSV) is an emerging pathogen associated with various diseases in swine. In this study, four PSV strains were isolated from diarrheic piglets and their genomes were analyzed. Monoclonal antibodies for PSV-VP1 protein and an indirect ELISA for the detection of PSV antibodies in serum were developed. These findings contribute to the understanding of PSV pathophysiology, development of diagnostic assays, and investigation of PSV epidemiology.
Article
Chemistry, Applied
Rongzhi Wang, Juncheng Wang, Haimei Liu, Yehong Gao, Qiang Zhao, Sumei Ling, Shihua Wang
Summary: The study identified a specific IgG against bovine LF for rapid detection of LF content in cow milk using ic-ELISA and lateral flow immunosensors, without the need for expensive equipment and sample pretreatment.