Article
Food Science & Technology
Africa Sanchiz, Paulina Sanchez-Enciso, Carmen Cuadrado, Rosario Linacero
Summary: This study used chloroplast markers and real-time PCR to enhance the sensitivity of peanut detection, applying different DNA extraction protocols, selecting appropriate primers and probes for detection, and ultimately determining that the mat k chloroplast marker was the most sensitive. The method is also capable of accurately detecting peanuts in processed foods, with a wide range of potential applications.
Article
Allergy
Stephen C. Dreskin, Stef J. Koppelman, Sandra Andorf, Kari C. Nadeau, Anjeli Kalra, Werner Braun, Surendra S. Negi, Xueni Chen, Catherine H. Schein
Summary: The study examines the immunologic basis for allergies to peanuts, tree nuts, and sesame seeds, focusing on the similarities among their 2S albumins. Research indicates that the 2S albumins play a crucial role as allergens in these allergies, and their common physicochemical properties may explain the observed coallergy.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Chemistry, Applied
Hai Jiang, Qian Guo, Cheng Zhang, Zhikang Sun, Xuan Weng
Summary: This study developed an origami microfluidic electrochemical nano-aptasensor for rapid peanut allergen detection. The sensor could complete detection within 20 min, with a linear range from 50 to 1000 ng/mL and a detection limit of 21.6 ng/mL, successfully detecting Ara h1 spiked cookie dough samples.
Article
Allergy
Alexandra F. Santos, Marcel Bergmann, Helen A. Brough, Natalia Couto-Francisco, Matthew Kwok, Valentina Panetta, Diab Haddad, Gideon Lack, Philippe Eigenmann, Jean-Christoph Caubet
Summary: The study demonstrates that BAT is a valuable tool in predicting allergic status and reducing the need for OFCs in children with nut or seed allergies, leading to a decrease in total OFCs and positive OFCs while maintaining a high diagnostic accuracy.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2021)
Article
Chemistry, Applied
Joost L. D. Nelis, James A. Broadbent, Utpal Bose, Alisha Anderson, Michelle L. Colgrave
Summary: This study improves the quantification of trace level peanut allergens in processed food using LC-MS by enhancing method robustness, total analysis time, and method sensitivity. The study optimized extraction buffers and peptide choice, and developed a rapid extraction and in-solution digestion method to reduce analysis time. The results showed accurate quantification, excellent linearity, and improved sensitivity compared to previous methods. These methodological improvements contribute to reliable peanut detection in food and can be applied to other foodborne allergens.
Article
Food Science & Technology
Clara Tramuta, Lucia Decastelli, Elisa Barcucci, Francesco Ingravalle, Sandra Fragassi, Samantha Lupi, Daniela Manila Bianchi
Summary: Food safety laboratories have developed a real-time PCR assay for the validation and accreditation of detecting peanut traces in food products. The method showed high sensitivity and specificity and was tested on different types of food matrices.
Article
Multidisciplinary Sciences
Megan Fay, Joelle K. Salazar, Padmini Ramachandran, Diana Stewart
Summary: Metagenomic analysis of pine nuts and sesame seeds revealed that these products are dominated by environmental bacterial genera and commensal organisms, which can aid in the risk assessment of these products by identifying food spoilage potential and community members which may co-enrich with foodborne bacterial pathogens.
Article
Chemistry, Applied
Robert M. Hnasko, Alice V. Lin, Jeffery A. McGarvey, Christopher P. Mattison
Summary: This study developed sensitive and selective immunoassays for the detection of peanut allergen Ara h1, which can provide a rapid surveillance method for peanut in food production and consumption.
Review
Allergy
Samuel Weiss, Derek Smith
Summary: Sesame allergy has been increasingly recognized as a major food allergen worldwide, with its prevalence, clinical presentation, natural history, allergenic epitopes, diagnosis, and treatment being extensively studied and documented. The United States will require sesame to be labeled as a food allergen starting from 2023, following the passage of relevant legislation. This review highlights the need for reliable methods of sesame allergy identification and management.
ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY
(2023)
Article
Food Science & Technology
Emilie Manny, Sebastien La Vieille, Virginie Barrere, Jeremie Theolier, Samuel Benrejeb Godefroy
Summary: Precautionary allergen labeling (PAL) is widely used in the food industry, but little is known about its occurrence in Canada. Peanuts and hazelnuts were found in 4% and 9% of analyzed products, with chocolates being mostly impacted.
NPJ SCIENCE OF FOOD
(2021)
Article
Cell Biology
Nada S. Alakhras, Jaeho Shin, Scott A. Smith, Anthony L. Sinn, Wenwu Zhang, Gyoyeon Hwang, Jenna Sjoerdsma, Emily K. Bromley, Karen E. Pollok, Basar Bilgicer, Mark H. Kaplan
Summary: Peanut-induced allergy is a type I hypersensitivity reaction mediated by IgE, which can range from local edema to life-threatening anaphylaxis. There are currently few preventive therapies for this type of allergy, but a covalent heterobivalent inhibitor (cHBI) has shown promise in both in vitro and in vivo models. In this study, a humanized mouse model expressing human mast cells was developed and used to demonstrate that cHBI can prevent allergic reactions, including fatal anaphylaxis, when administered before or shortly after allergen exposure.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Review
Food Science & Technology
Chang Chen, Zhongli Pan
Summary: This comprehensive review summarizes the scientific research and industrial processing practices of almonds, walnuts, and pistachios, focusing on the processes of drying, disinfection, disinfestation, and downstream processes. It also discusses new perspectives and applications of tree nut processing waste and by-products and highlights future trends and research needs.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Food Science & Technology
Joseph Touma, Silvia Dominguez, Sebastien La Vieille, Benjamin C. Remington, Joseph L. Baumert, Jeremie Theolier, Samuel Benrejeb Godefroy
Summary: Despite the lack of studies on sesame allergens in the Middle East, a research in Lebanon showed that bakery products, particularly sfouf, pose a serious risk to sesame-allergic children and adolescents. Guidance on the use of Product Advisory Labels (PAL) is needed to better protect allergic consumers in the region.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Allergy
Vicki L. McWilliam, Jennifer J. Koplin, Katie Allen, Marnie Robinson, Joanne Smart, Paxton Loke, Rachel L. Peters, Thanh Dang, Katherine J. Lee, Kim Dalziel, Dean Tey, Mark Taranto, Kirsten P. Perrett
Summary: TreEAT is a trial that aims to test the efficacy and safety of a novel strategy, a supervised multi-nut oral food challenge, for the prevention of tree nut allergies in high-risk infants. The trial will compare this strategy with standard care for tree nut allergy prevention.
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2023)
Review
Agriculture, Multidisciplinary
Yaozhong Hu, Yi Wang, Jing Lin, Sihao Wu, Serge Muyldermans, Shuo Wang
Summary: The unique characteristics of camelid heavy-chain only antibodies (HCAb) derived nanobodies (Nbs) have led to their widespread application in various fields. However, their application in food allergen inspection and immunotherapy has been lacking. Nbs possess strong properties and a simple selecting strategy, making them advantageous for immunoassays and as blocking antibodies. The development of specific Nbs against food allergens shows their potential in food allergen surveillance and immunotherapy.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
