Article
Agriculture, Dairy & Animal Science
Lourenco Ramos-Pinto, Marina Machado, Josep Calduch-Giner, Jaume Perez-Sanchez, Jorge Dias, Luis E. C. Conceicao, Tome S. Silva, Benjamin Costas
Summary: The study aimed to evaluate the effects of histidine, taurine, and threonine supplementation on the immune status of gilthead seabream. The results showed that dietary supplementation with these amino acids had mild immune-modulation effects on gilthead seabream, particularly noteworthy for further study under disease challenge conditions.
Article
Veterinary Sciences
Concetta Maria Messina, Simona Manuguerra, Rosaria Arena, Cristobal Espinosa-Ruiz, Eleonora Curcuraci, Maria Angeles Esteban, Andrea Santulli
Summary: Chemical contaminants such as heavy metals, PBDEs and drugs are commonly found in the marine environment, and their effects on animal welfare, food safety and security have attracted the attention of the scientific community. This study examined the effects of a mixture of carbamazepine, PBDE-47 and cadmium chloride on Sparus aurata specimens, analyzing oxidative stress in the liver and the quality of the fish in terms of fatty acid profile and lipid peroxidation.
RESEARCH IN VETERINARY SCIENCE
(2023)
Article
Fisheries
Daniel Gonzalez-Silvera, Alberto Cuesta, Maria Angeles Esteban
Summary: This study identified and characterized different humoral defence mechanisms in the liver and bile of gilthead seabream for the first time, finding that bile contained higher levels of proteases, esterase, and antioxidant enzymes with potent bacteriostatic activity against pathogenic bacteria, while the liver only showed low levels of antioxidant enzymes.
JOURNAL OF FISH BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Rosaria Arena, Adja Cristina Lira de Medeiros, Giulia Secci, Simone Mancini, Simona Manuguerra, Fulvia Bovera, Andrea Santulli, Giuliana Parisi, Concetta Maria Messina, Giovanni Piccolo
Summary: In recent years, there has been increasing interest in the use of natural compounds, such as honeybee pollen (HBP), as fish health promoters in aquaculture. This study investigated the effects of HBP and its extract on immune response and quality parameters in farmed gilthead seabream. The results showed that a diet containing HBP extract at a certain level improved fish health parameters without impacting fillets quality, suggesting its potential as an immunostimulant for gilthead seabream.
Article
Fisheries
Ana Galindo, Jose A. Perez, Virginia Martin, Nieves G. Acosta, Diana B. Reis, Ignacio A. Jimenez, Goncalo Rosa, Marianna Venuleo, Manuel Marrero, Covadonga Rodriguez
Summary: This study assesses the use of beach-cast macroalgae as a dietary supplement for gilthead seabream juveniles. The results show that the inclusion of 7% macroalgae in the diet has no negative effect on the growth and health of the fish and can increase the content of beneficial substances in the muscle.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Marta Carvalho, Daniel Montero, Silvia Torrecillas, Pedro Castro, Marta Jesus Zamorano, Marisol Izquierdo
Summary: This study investigated how the combination of poultry oil with microalgae oils could modulate hepatic lipid metabolism in gilthead sea bream juveniles. It was found that combining microalgae with poultry oil could be an alternative lipid and essential fatty acid source to fish oil in marine fish diets.
Article
Fisheries
Sam J. S. Houston, Vasileios Karalazos, John Tinsley, Douglas R. Tocher, Brett D. Glencross, Oscar Monroig
Summary: Carnivorous marine fish species such as gilthead seabream require dietary EPA and DHA acids for optimal growth. However, the limited supply of marine oils in aquafeeds due to the rapid growth of global aquaculture has led to the use of vegetable oils lacking EPA and DHA. Therefore, the EFA requirements of juvenile marine fish need to be reassessed. A dietary trial with gilthead seabream demonstrated that the EFA requirement is a function of fish mass, decreasing as the fish grows, and may be as high as 2% when considering feed conversion ratio. Different regression models were explored to determine nutrient requirement estimates, with the split linear model fitting the data best for both pellet sizes.
Article
Biotechnology & Applied Microbiology
Alba Galafat, Antonio Jesus Vizcaino, Maria Isabel Saez, Juan Luis Gomez-Pinchetti, Francisco Gabriel Acien, Tomas Francisco Martinez, Francisco Javier Alarcon
Summary: This study evaluated the potential dietary ingredients of different microalgae and cyanobacteria in aquafeeds. The results showed that all the evaluated microalgae and cyanobacteria had high protein content and a balanced amino acid profile.
JOURNAL OF APPLIED PHYCOLOGY
(2022)
Article
Horticulture
Safoora Saadati, Bahram Baninasab, Mostafa Mobli, Mahdiyeh Gholami
Summary: This study found that foliar application of MeJA and EBR can significantly increase freezing tolerance in olive leaves, with improvements in soluble carbohydrates, proline, total soluble proteins, antioxidant enzymes, DPPH radical scavenging capacity, and total phenolic content. Notably, the highest freezing tolerance was observed in EBR 0.6 mu M treatment and the lowest in the control group.
SCIENTIA HORTICULTURAE
(2021)
Article
Physiology
Mariana Ferreira, Pedro C. Ribeiro, Laura Ribeiro, Marisa Barata, Valentina F. Domingues, Sara Sousa, Cristina Soares, Alexandra Marques, Pedro Pousao-Ferreira, Jorge Dias, L. Filipe C. Castro, Antonio Marques, Maria L. Nunes, Luisa M. P. Valente
Summary: This study evaluated the impact of biofortified diets containing microalgae, macroalgae, and selenised yeast on gilthead seabream. The results showed that microalgae can partially replace fish meal and fish oil in the diets for seabream. The addition of macroalgae and selenised yeast should be controlled at proper levels. The muscle of seabream fed with microalgae diets had higher EPA and DHA content, and their retention and gain of selenium were also increased. However, more research is needed for iodine fortification in fish.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Environmental Sciences
Antonia Solomando, Xavier Capo, Carme Alomar, Montserrat Compa, Jose Maria Valencia, Antoni Sureda, Salud Deudero
Summary: Exposure to LDPE-MPs had significant impacts on oxidative stress and inflammation biomarkers in the liver and plasma of Sparus aurata, while no significant changes were observed in erythrocytes. During the depuration period, most of these biomarkers showed a trend of recovery and reached levels similar to those of the control group. This indicates a certain degree of reversibility in the effects of LDPE-MPs on S. aurata.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Chemistry, Medicinal
Concetta Maria Messina, Rosaria Arena, Simona Manuguerra, Giuseppe Renda, Vincenzo Alessandro Laudicella, Giovanna Ficano, Gioacchino Fazio, Laura La Barbera, Andrea Santulli
Summary: This study demonstrates a pilot scale protocol for obtaining omega 3-enriched oil from farmed gilthead sea bream viscera, which shows bioactivity and good quality for animal and human consumption. The extracted TFA ethyl esters enriched in PUFAE exhibit antioxidant activities and anti-adipogenic effects, proving beneficial for both farmed fish and human health. These results may promote the recovery and utilization of aquaculture by-products on a larger scale and contribute to the development of more sustainable business models in aquaculture resource utilization.
Article
Fisheries
Norhan E. Saleh, Nevien Abdelkhalek, Samah Lashen, Eman Abbas
Summary: Supplementation of inosine (I) and uracil (U) in sea bream fingerlings' diet enhances growth, improves intestinal health, boosts immune response, increases resistance to stress exposure, and reduces oxidative stress levels.
AQUACULTURE RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Filomena Del Piano, Adriano Lama, Anna Monnolo, Claudio Pirozzi, Giovanni Piccolo, Simone Vozzo, Davide De Biase, Lorenzo Riccio, Giovanna Fusco, Raffaelina Mercogliano, Rosaria Meli, Maria Carmela Ferrante
Summary: Plastics are a major pollutant in the sea, breaking down into microplastic particles that can cause toxic effects in aquatic organisms. This study focused on the gilthead seabream and found that ingestion of microplastics increases oxidative and nitrosative stress in the intestine, impairing the antioxidant defense system. This damage to the gut can lead to health issues in fish.
Article
Fisheries
Jose Carlos Campos-Sanchez, Daniel Gonzalez-Silvera, Xu Gong, Richard Broughton, Francisco A. Guardiola, Monica B. Betancor, Maria Angeles Esteban
Summary: The role of adipocytes and fatty acids in carrageenan-induced skin inflammation was studied in gilthead seabream. Results showed that injection of lambda-carrageenin led to an increase in adipocyte area at 3 hours post-injection and an increase in adipocyte frequency at 6 hours post-injection. Analysis of lipid classes revealed increased levels of free fatty acids and sphingomyelin in the injected fish compared to the control group. The fatty acid profile of the fish skin also showed changes, with an increase in saturated fatty acids and n-6 polyunsaturated fatty acids, and a decrease in monounsaturated fatty acids, n-3 polyunsaturated fatty acids, and minor fatty acids at 6 hours after injection. Evaluation of eicosanoids indicated an increase in hydroxyeicosatetraenoic acid in the injected fish compared to the control group. These findings demonstrate the contribution of adipocytes and fatty acids to the inflammatory response in gilthead seabream skin.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Fernando Naya-Catala, M. Carla Piazzon, Josep A. Calduch-Giner, Ariadna Sitja-Bobadilla, Jaume Perez-Sanchez
Summary: The present study used a metatranscriptomic approach to investigate the expression of gut microbial genes in farmed gilthead sea bream. The researchers found that fungal transcripts were as abundant as bacterial ones, contrary to previous rRNA amplicon-based studies. The study also revealed that dietary intervention and different growth rates of fish influenced the metatranscriptome, with bacteria primarily shaping vitamin and amino acid metabolism and rhythmic and symbiotic processes, while fungi played a more specific role in configuring host immune, digestive, and endocrine processes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
M. C. Piazzon, F. Naya-Catala, G. V. Pereira, I. Estensoro, R. Del Pozo, J. A. Calduch-Giner, W. G. Nuez-Ortin, O. Palenzuela, A. Sitja-Bobadilla, J. Dias, L. E. C. Conceicao, J. Perez-Sanchez
Summary: This study evaluated the effects of the NoPAP SANA diet on gilthead sea bream, showing that the diet promotes optimal growth and a healthy condition without affecting susceptibility to intestinal parasites.
Article
Genetics & Heredity
Ahmed Ibrahem Hafez, Beatriz Soriano, Aya Allah Elsayed, Ricardo Futami, Raquel Ceprian, Ricardo Ramos-Ruiz, Genis Martinez, Francisco Jose Roig, Miguel Angel Torres-Font, Fernando Naya-Catala, Josep Alvar Calduch-Giner, Lucia Trilla-Fuertes, Angelo Gamez-Pozo, Vicente Arnau, Jose Maria Sempere-Luna, Jaume Perez-Sanchez, Toni Gabaldon, Carlos Llorens
Summary: The GPRO suite is an ongoing bioinformatic project for the analysis of -omics data. It introduces a client- and server-side solution for comparative transcriptomics and variant analysis. The client-side includes two Java applications, RNASeq and VariantSeq, which manage pipelines and workflows using common command line tools. The server-side, named GPRO Server-Side, hosts all dependencies and requires a Linux operating system, PHP, SQL, Python, bash scripting, and third-party software. The solution offers both desktop and web applications, with step-by-step and pipeline modes for execution. It also features an experimental support system called GENIE, with a chatbot assistant and expert system for troubleshooting and recommendations.
Article
Biochemistry & Molecular Biology
Carmen Tatiana Kalinowski, Monica B. Betancor, Silvia Torrecillas, Matthew Sprague, Laurence Larroquet, Vincent Veron, Stephane Panserat, Maria Soledad Izquierdo, Sadasivam J. Kaushik, Stephanie Fontagne-Dicharry
Summary: This study investigated the influence of dietary astaxanthin (AX) on glucose and lipid metabolism in rainbow trout liver. The results showed that dietary AX had a significant impact on neutral and polar lipids in the liver, hepatocyte size, cytoplasm vacuolization, and gene expression related to TAG turnover, beta-oxidation, PL and cholesterol synthesis. Overall, dietary AX modulates the oxidative phase of the pentose phosphate pathway and the last step of glycolysis, affecting various aspects of lipid metabolism in rainbow trout liver.
Article
Fisheries
Socorro Toxqui-Rodriguez, Fernando Naya-Catala, Ariadna Sitja-Bobadilla, M. Carla Piazzon, Jaume Perez-Sanchez
Summary: This study compared different sequencing platforms for microbial characterization in fish gut mucosal samples. The results showed that MinION is a reliable and accurate tool for assessing intestinal bacteria communities, with good correlation with Illumina results. However, less abundant but biologically important taxa may be masked compared to Illumina sequencing.
Article
Fisheries
Enrique Riera-Ferrer, Raquel Del Pozo, M. Carla Piazzon, Ariadna Sitja-Bobadilla, Itziar Estensoro, Oswaldo Palenzuela
Summary: This study explores the best experimental conditions to set up an in vivo infection model capable of mimicking the sparicotylosis signs observed in farmed diseased fish. The experimental setup for parasite transmission consisted of a recipient fish tank with naive GSB receiving water from two S. chrysophrii-infected donor tanks. The study provides an advancement for studying S. chrysophrii's biology and its interaction with its host, as well as for further researching treatment alternatives and prophylactic measures.
Article
Biodiversity Conservation
Yaiza Fernandez-Palacios, Sadasivam Kaushik, Andrej Abramic, Victor Cordero-Penin, Alejandro Garcia-Mendoza, Alberto Bilbao-Sieyro, Yeray Perez-Gonzalez, Pedro Sepulveda, Isabel Lopes, Carlos Andrade, Natacha Nogueira, Gilberto P. Carreira, Maria Magalhaes, Ricardo Haroun
Summary: This study aims to assess the current state and future development trends of the main maritime sectors related to the blue economy in the European Macaronesia Sea Basin, and provide a basis for maritime spatial planning processes at a regional level. The results show that some maritime sectors are well established while others have varying long-term growth potential. The European Macaronesia example has the potential to act as a good practice for promoting sustainable blue economy in other similar geographical settings.
JOURNAL OF COASTAL CONSERVATION
(2023)
Article
Physiology
Paul G. Holhorea, Fernando Naya-Catala, Alvaro Belenguer, Josep A. Calduch-Giner, Jaume Perez-Sanchez
Summary: This study combines multiple systems to understand the schooling behavior and adaptive features of farmed gilthead sea bream in high stocking density conditions. The results show that high density fish exhibit adaptive behavior, such as changes in feeding behavior and enhanced endogenous swimming activity rhythm, but also experience reduced growth rates and limited oxygen availability.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Genetics & Heredity
Beatriz Soriano, Ahmed Ibrahem Hafez, Fernando Naya-Catala, Federico Moroni, Roxana Andreea Moldovan, Socorro Toxqui-Rodriguez, Maria Carla Piazzon, Vicente Arnau, Carlos Llorens, Jaume Perez-Sanchez
Summary: This paper introduces SAMBA, a computational tool that uses a Bayesian network approach to model the network structure and interactions of fish gut microbiomes in aquaculture systems. SAMBA can infer the influence of farming conditions on microbiome diversity and predict changes under different conditions. It is worth noting that SAMBA can be used for modeling microbiome-host network relationships of any vertebrate organism, including humans, in any system and/or ecosystem.
Article
Immunology
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: This study investigated the potential of using a combination of pungent spices as a dietary supplement for gilthead seabream. The results showed that the supplement enhanced growth performance, improved lipid metabolism, and reduced fat accumulation in various tissues. It can be used as part of a nutritional strategy to promote a more judicious use of fish oil in fish diets.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
F. Naya-Catala, A. Belenguer, D. Montero, S. Torrecillas, B. Soriano, J. Calduch-Giner, C. Llorens, R. Fontanillas, S. Sarih, M. J. Zamorano, M. Izquierdo, J. Perez-Sanchez
Summary: The study found that the influence of broodstock nutrition on gilthead sea bream with different genetic backgrounds results in distinct hepatic transcriptome and genome-wide DNA methylation patterns, with the magnitude of the impact depending on the genetic background.
Meeting Abstract
Fisheries
Itziar Estensoro, Raquel Del Pozo, Ariadna Sitja-Bobadilla, M. Carla Piazzon
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Meeting Abstract
Fisheries
E. Riera-Ferrer, E. Gimeno, R. Del Pozo, O. Palenzuela, M. C. Piazzon, A. Sitja-Bobadilla, I. Estensoro
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)