Article
Chemistry, Analytical
Fangbin Xiao, Ju Liu, Xiaoyan Feng, Guoyang Xie, Wanqing Zhang, Hengyi Xu
Summary: This study developed a method based on magnetic nanoparticles and recombinase aided amplification for rapid capture and detection of Staphylococcus aureus in food samples. The method has low cost, short detection time, and high sensitivity, and can simultaneously analyze multiple bacteria, which is important for evaluating the microbiological safety of food samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Engineering, Electrical & Electronic
Zhangliang Xu, Yang Luo
Summary: This paper describes an immunoglobulin G (IgG) biofunctionalized quartz crystal microbalance (QCM) sensor for Staphylococcus aureus (S. aureus) sensing applications. The experimental results show that the IgG-based QCM sensor exhibits good sensitivity performance and has a higher sensitivity in liquid phase compared to air.
IEEE SENSORS JOURNAL
(2022)
Article
Microbiology
Runan Cheng, Lei Li, Sihui Zhen, Honglei Liu, Zhouhui Wu, Yu Wang, Zhen Wang
Summary: In this study, a novel method based on immunomagnetic separation using immunomagnetic beads (IMBs) and recombinase polymerase amplification (RPA) was developed for the rapid detection of Staphylococcus aureus (S. aureus) in milk and pork. The IMBs-RPA method showed potential for food safety supervision and rapid early diagnosis of diseases due to its short detection time, high sensitivity, and specificity.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Wei Chen, Yanke Zhang, Qingteng Lai, Youzhen Li, Zhengchun Liu
Summary: In this study, a fluorometric aptasensor for the rapid and accurate detection of Staphylococcus aureus was designed. The aptasensor demonstrated ultra-high sensitivity, specificity, and fast detection speed. By utilizing multiple amplification mechanisms, the concentration of S. aureus could be precisely quantified. The recovery test also confirmed the effectiveness of the aptasensor in detecting S. aureus in spiked blood samples. The study highlights the potential of aptasensors based on multiple amplification for practical, high-performance bacterial detection biosensors.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Applied
Wenjie Yu, Yanjing Chen, Zhiying Wang, Lu Qiao, Ruibin Xie, Juan Zhang, Suying Bian, Hui Li, Yan Zhang, Ailiang Chen
Summary: A real-time fluorescent multiplex LAMP assay based on a centrifugal microfluidic chip was developed to simultaneously detect milk from cow, camel, horse, goat, and yak. The assay simplifies the detection process, reduces false-positive results, and has a genomic detection limit that meets the authentication requirements for detecting and differentiating common milk species.
Article
Food Science & Technology
Yanan Shi, Yufang Li, Kun Yang, Guangqiang Wei, Aixiang Huang
Summary: The novel antimicrobial agent BCp12 derived from buffalo milk has been found to possess superior antimicrobial activity against Staphylococcus aureus. This study investigated the effect of BCp12 on the cell wall synthesis and molecular pathways of Staphylococcus aureus through various analyses. The results showed that BCp12 damaged the cell wall integrity and resulted in pore formation, and also affected different molecular functions and pathways. The study also verified the expression of related proteins and genes. Overall, BCp12 demonstrates promising potential for application in food preservation due to its dual-targeted effects against S. aureus in milk.
Article
Agriculture, Dairy & Animal Science
Jiai Yan, Ruijin Yang, Suhuai Yu, Wei Zhao
Summary: Staphylococcus aureus is a prevalent foodborne pathogen that poses a threat to human health, especially with the emergence of multidrug-resistant strains like MRSA. The study found that the endolysin LysGH15 and its catalytic domain CHAP(LysGH15) exhibited strong antimicrobial efficacy against S. aureus, with differing optimal activity conditions. The sensitivity to NaCl concentration also varied between LysGH15 and CHAP(LysGH15), affecting their lytic activity levels.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Microbiology
Colin Wood, Jason Sahl, Sara Maltinsky, Briana Coyne, Benjamin Russakoff, David Panisello Yague, Jolene Bowers, Talima Pearson
Summary: Existing molecular assays for Staphylococcus aureus detection lack sensitivity and specificity. The newly developed qPCR assay SaQuant shows high sensitivity and specificity, with potential for accurate detection and quantification of S. aureus. It has the ability to better understand the dynamics of prevalence and transmission in community settings.
Article
Microbiology
Ryota Miyazawa, So Shimoda, Keiichi Matsuda, Ryuta Tobe, Tasuke Ando, Hiroshi Yoneyama
Summary: Staphylococcus aureus is a major pathogen in humans and livestock, causing serious issues such as bovine mastitis. This study monitored cases of intramammary infection caused by S. aureus in the Miyagi Prefecture, Japan, and analyzed the strains obtained from dairy farms. The study found recurrent cases of infection and identified a Methicillin-Sensitive SA (MSSA) ST398 strain isolated from mastitis-containing cows, which is the first confirmed case in Japan. The results highlight the importance of nationwide surveillance to manage the distribution of ST398 strains in dairy farms for human and animal health.
Article
Agriculture, Dairy & Animal Science
Xuelong Chen, Honghai Wang, Chang Liu, Yanping Qi, Weina Guo
Summary: The study established a closed-tube isothermal multiple self-matching-initiated amplification (IMSA) technique for visually confirming the presence of Staph. aureus in milk samples. The method successfully amplified the target sequence under optimized conditions with high specificity and sensitivity.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Qin Ouyang, Mingming Zhang, Yongcun Yang, Zia-ud Din, Quansheng Chen
Summary: In this study, a novel fluorescence biosensor was designed for the rapid detection of Staphylococcus aureus in meat. By using mesoporous silica-modified upconversion nanoparticles, the biosensor achieved low detection limits and wide detection range. The results showed that the biosensor had comparable performance to the traditional plate counting method in detecting S. aureus in meat.
Article
Food Science & Technology
Wajeehah Wajahat, Z. R. Azaz Ahmad Azad, Sadaf Nazir, Gazia Nasir
Summary: The study evaluated the use of RT-PCR to detect adulteration of camel milk, successfully amplifying camel specific primers. The results indicated positive amplification for camel milk and its products, demonstrating the effectiveness and practicality of this method for identification.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Biotechnology & Applied Microbiology
Ya-Lei Wang, Xin Zhang, Quan Wang, Peng-Xuan Liu, Wei Tang, Rong Guo, Hai-Yang Zhang, Zhao-Guo Chen, Xian-Gan Han, Wei Jiang
Summary: This study developed a novel approach combining lateral flow recombinase polymerase amplification (RPA-LF) with immunomagnetic separation (IMS) for rapid detection of Staphylococcus aureus in milk. The method showed high sensitivity, specificity, and speed, detecting as low as 40 CFU per reaction in milk samples within a total testing time of 70 minutes.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Zhonglin Shi, Yanan Li, Anzhong Hu, Junsheng Cui, Min Shao, Ling Zhu, Ke Yang, Yong Liu, Guoqing Deng, Cancan Zhu
Summary: In this study, a rapid and accurate drug-resistant Staphylococcus aureus (S. aureus) detection technology was developed by combining fluorescent probes with recombinase polymerase amplification (RPA). The method showed a low detection limit and high specificity for detecting two resistance genes in drug-resistant S. aureus.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Article
Food Science & Technology
Zhenzhen Zhang, Yanmin Song, Liyan Ma, Kunlun Huang, Zhihong Liang
Summary: This study investigated the presence of Staphylococcus aureus and Ochratoxin A (OTA) in pasteurized milk in ten urban Beijing districts, and found the co-occurrence of these two biohazards. The results indicate that when the cold chain is not guaranteed, the presence of S. aureus carrying enterotoxin genes in milk poses a risk to food safety, while the high detection rates and levels of OTA suggest it is a hidden risk.
Article
Microbiology
Karel Brinda, Alanna Callendrello, Kevin C. Ma, Derek R. MacFadden, Themoula Charalampous, Robyn S. Lee, Lauren Cowley, Crista B. Wadsworth, Yonatan H. Grad, Gregory Kucherov, Justin O'Grady, Michael Baym, William P. Hanage
NATURE MICROBIOLOGY
(2020)
Article
Cell & Tissue Engineering
A. J. Trotter, R. Dean, C. E. Whitehouse, J. Mikalsen, C. Hill, R. Brunton-Sim, G. L. Kay, M. Shakokani, A. Z. E. Durst, J. Wain, I McNamara, J. O'Grady
BONE & JOINT RESEARCH
(2020)
Article
Infectious Diseases
Erik Alm, Eeva K. Broberg, Thomas Connor, Emma B. Hodcroft, Andrey B. Komissarov, Sebastian Maurer-Stroh, Angeliki Melidou, Richard A. Neher, Aine O'Toole, Dmitriy Pereyaslov
Article
Biology
Kate Reddington, David Eccles, Justin O'Grady, Devin M. Drown, Lars Hestbjerg Hansen, Tue Kjaergaard Nielsen, Anne-Lise Ducluzeau, Richard M. Leggett, Darren Heavens, Ned Peel, Terrance P. Snutch, Anthony Bayega, Spyridon Oikonomopoulos, Jiannis Ragoussis, Thomas Barry, Eric van der Helm, Dino Jolic, Hollian Richardson, Hans Jansen, John R. Tyson, Miten Jain, Bonnie L. Brown
Review
Public, Environmental & Occupational Health
A. P. Wagner, V. Enne, D. M. Livermore, J. Craig, D. A. Turner
JOURNAL OF HOSPITAL INFECTION
(2020)
Article
Genetics & Heredity
Ebenezer Foster-Nyarko, Nabil-Fareed Alikhan, Anuradha Ravi, Gaetan Thilliez, Nicholas M. Thomson, David Baker, Gemma Kay, Jennifer D. Cramer, Justin O'Grady, Martin Antonio, Mark J. Pallen
MICROBIAL GENOMICS
(2020)
Article
Multidisciplinary Sciences
Louis du Plessis, John T. McCrone, Alexander E. Zarebski, Verity Hill, Christopher Ruis, Bernardo Gutierrez, Jayna Raghwani, Jordan Ashworth, Rachel Colquhoun, Thomas R. Connor, Nuno R. Faria, Ben Jackson, Nicholas J. Loman, Aine O'Toole, Samuel M. Nicholls, Kris Parag, Emily Scher, Tetyana Vasylyeva, Erik M. Volz, Alexander Watts, Isaac I. Bogoch, Kamran Khan, David M. Aanensen, Moritz U. G. Kraemer, Andrew Rambaut, Oliver G. Pybus
Summary: The study analyzed the genetic lineage structure of the early UK COVID-19 epidemic through genomic sampling, identifying that virus lineages introduced before the national lockdown tended to be larger and more dispersed. After the lockdown, lineage importation and regional diversity decreased, while lineage elimination depended on lineage size. The research emphasizes the impact of genetic perspective on transmission dynamics for COVID-19 epidemiology and control.
Article
Multidisciplinary Sciences
Ebenezer Foster-Nyarko, Nabil-Fareed Alikhan, Usman N. Ikumapayi, Golam Sarwar, Catherine Okoi, Peggy-Estelle Maguiagueu Tientcheu, Marianne Defernez, Justin O'Grady, Martin Antonio, Mark J. Pallen
Summary: The study reveals the genomic diversity of Escherichia coli in asymptomatic children from rural Gambia, with 56 genotypes identified and an average of 2.7 genotypes per host. Most diversity is attributed to immigration events, while a minority arises from within-host evolution. Many isolates carry virulence factors and resistance genes, indicating a potential reservoir for these genes. The study also highlights the importance of immigration and strain establishment in generating diversity, with only a minor role played by within-host evolution.
Article
Genetics & Heredity
Ebenezer Foster-Nyarko, Nabil-Fareed Alikhan, Anuradha Ravi, Nicholas M. Thomson, Sheikh Jarju, Brenda A. Kwambana-Adams, Arss Secka, Justin O'Grady, Martin Antonio, Mark John Pallen
Summary: The study revealed that E. coli isolates from chickens and guinea fowl in Gambian homes harbor antimicrobial resistance genes, particularly the ST155 strain showing multidrug resistance to clinically relevant antibiotics, indicating potential pathogenicity. Moreover, close relatedness between Gambian poultry strains and human isolates suggests a risk of transmission.
MICROBIAL GENOMICS
(2021)
Article
Multidisciplinary Sciences
Steven A. Kemp, Dami A. Collier, Rawlings P. Datir, Isabella A. T. M. Ferreira, Salma Gayed, Aminu Jahun, Myra Hosmillo, Chloe Rees-Spear, Petra Mlcochova, Ines Ushiro Lumb, David J. Roberts, Anita Chandra, Nigel Temperton, Katherine Sharrocks, Elizabeth Blane, Yorgo Modis, Kendra E. Leigh, John A. G. Briggs, Marit J. van Gils, Kenneth G. C. Smith, John R. Bradley, Chris Smith, Rainer Doffinger, Lourdes Ceron-Gutierrez, Gabriela Barcenas-Morales, David D. Pollock, Richard A. Goldstein, Anna Smielewska, Jordan P. Skittrall, Theodore Gouliouris, Ian G. Goodfellow, Effrossyni Gkrania-Klotsas, Christopher J. R. Illingworth, Laura E. McCoy, Ravindra K. Gupta
Summary: Chronic infection with SARS-CoV-2 can lead to viral evolution and increased resistance to neutralizing antibodies in immunosuppressed individuals treated with convalescent plasma. During convalescent plasma therapy, there were significant shifts in the viral population structure and sensitivity, suggesting strong selection pressure on the virus during treatment.
Article
Genetics & Heredity
Dave J. Baker, Alp Aydin, Thanh Le-Viet, Gemma L. Kay, Steven Rudder, Leonardo de Oliveira Martins, Ana P. Tedim, Anastasia Kolyva, Maria Diaz, Nabil-Fareed Alikhan, Lizzie Meadows, Andrew Bell, Ana Victoria Gutierrez, Alexander J. Trotter, Nicholas M. Thomson, Rachel Gilroy, Luke Griffith, Evelien M. Adriaenssens, Rachael Stanley, Ian G. Charles, Ngozi Elumogo, John Wain, Reenesh Prakash, Emma Meader, Alison E. Mather, Mark A. Webber, Samir Dervisevic, Andrew J. Page, Justin O'Grady
Summary: CoronaHiT is a flexible sequencing method for SARS-CoV-2 genomes, suitable for rapid expansion of global sequencing efforts. In experiments, CoronaHiT demonstrated good performance on both nanopore and Illumina platforms.
Article
Microbiology
Tianqi Li, Enriqueta Garcia-Gutierrez, Daniel A. Yara, Jacob Scadden, Jade Davies, Chloe Hutchins, Alp Aydin, Justin O'Grady, Arjan Narbad, Stefano Romano, Lizbeth Sayavedra
Summary: This study aimed to establish a sensitive and reliable method for detecting SARS-CoV-2 in stool samples. Through ultrafiltration and RNA extraction, the virus was detected in most samples, with the primer set targeting the N1 region providing more reliable results and a limit of detection of 1 viral particle per mg of stool despite high variability at low viral titres.
Article
Biochemistry & Molecular Biology
Owen Higgins, Terry J. Smith
Summary: PCR is crucial for infectious disease detection, especially during the COVID-19 pandemic. Real-time multiplex detection and single-base specificity play key roles in identifying single-nucleotide polymorphisms (SNPs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Nabil-Fareed Alikhan, Luisa Zanolli Moreno, Luis Ricardo Castellanos, Marie Anne Chattaway, Jim McLauchlin, Martin Lodge, Justin O'Grady, Roxana Zamudio, Emma Doughty, Liljana Petrovska, Marcos Paulo Vieira Cunha, Terezinha Knobl, Andrea Micke Moreno, Alison E. Mather
Summary: The most prevalent serovars in Brazilian poultry and in poultry products imported from Brazil into the UK were Salmonella Heidelberg and Salmonella Minnesota. However, these serovars did not lead to an increase in clinical disease cases in the UK. They shared resistance to certain antibiotics not widely observed in other circulating serovars. Introduction of the Salmonella Enteritidis vaccine and increased antimicrobial use in Brazilian poultry could have resulted in replacement of drug-resistant serovars that are less associated with disease in humans in the UK.
Article
Infectious Diseases
Vera Manageiro, Vanessa Salgueiro, Tania Rosado, Narcisa M. Bandarra, Eugenia Ferreira, Terry Smith, Elsa Dias, Manuela Canica
Summary: This study analyzed the resistome, virulome, and mobilome of an MCR-9-producing Enterobacter strain. The results highlight the spread of antibiotic resistance in aquaculture through food, the environment, and humans, posing a serious threat to human health.
