Journal
BIOCONJUGATE CHEMISTRY
Volume 21, Issue 6, Pages 1018-1022Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc100083d
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Funding
- U.S. Department of Energy [LLNL-JRNL-420436, DE-AC52-07NA27344]
- Lawrence Livermore National Laboratory [LDRD 06-S1-003, LDRD 09-LW-077]
- NIAID through the Western Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research (NIH) [U54 AI057156, R21 AI077077]
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Subunit antigens are attractive candidates for vaccine development, as they are safe, cost-effective, and rapidly produced. Nevertheless, subunit antigens often need to be adjuvanted and/or formulated to produce products with acceptable potency and efficacy. Here, we describe a simple method for improving the potency and efficacy of a recombinant subunit antigen by its immobilization on nickel-chelating nanolipoprotein particles (NiNLPs). NiNLPs are membrane mimetic nanoparticles that provide a delivery and presentation platform amenable to binding any recombinant subunit immunogens featuring a polyhistidine tag. A His-tagged, soluble truncated form of the West Nile virus (WNV) envelope protein (trE-His) was immobilized on NiNLPs. Single inoculations of the NiNLP-trE-His produced superior anti-WNV immune responses and provided significantly improved protection against a live WNV challenge compared to mice inoculated with trE-His alone. These results have broad implications in vaccine development and optimization, as NiNLP technology is well-suited to many types of vaccines, providing a universal platform for enhancing the potency and efficacy of recombinant subunit immunogens.
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