Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization

Resolution of the interaction mechanisms and characteristics of non-nucleoside inhibitors of hepatitis C virus polymerase

(2013) Johan Winquist et al.   ANTIVIRAL RESEARCH

Bromo-deaza-SAH: A potent and selective DOT1L inhibitor

(2013) Wenyu Yu et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Structural and biochemical characterization of an atypical short-chain dehydrogenase/reductase reveals an unusual cofactor preference

(2013) Géraldine Buysschaert et al.   FEBS Journal

Pharmacological Chaperones as Therapeutics for Lysosomal Storage Diseases

(2013) Robert E. Boyd et al.   JOURNAL OF MEDICINAL CHEMISTRY

Computational identification of a transiently open L1/S3 pocket for reactivation of mutant p53

(2013) Christopher D. Wassman et al.   Nature Communications

The stability of 2-state, 3-state and more-state proteins from simple spectroscopic techniques... plus the structure of the equilibrium intermediates at the same time

(2012) Javier Sancho   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Small-molecule inhibition of MLL activity by disruption of its interaction with WDR5

(2012) Guillermo Senisterra et al.   BIOCHEMICAL JOURNAL

Real-time protein unfolding: a method for determining the kinetics of native protein denaturation using a quantitative real-time thermocycler

(2012) Kyle Biggar et al.   BIOTECHNIQUES

Correction of Both NBD1 Energetics and Domain Interface Is Required to Restore ΔF508 CFTR Folding and Function

(2012) Wael M. Rabeh et al.   CELL

Assessing the Differential Affinity of Small Molecules for Noncanonical DNA Structures

(2012) Loic Stefan et al.   CHEMBIOCHEM

Thermodynamic studies for drug design and screening

(2012) Nichola C Garbett et al.   Expert Opinion on Drug Discovery

Ligand–receptor interaction platforms and their applications for drug discovery

(2012) Ye Fang   Expert Opinion on Drug Discovery

EvaluatingDrosophilap53 as a Model System for Studying Cancer Mutations

(2012) Gal Herzog et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Discovery, Structure–Activity Relationship, and Biological Evaluation of Noninhibitory Small Molecule Chaperones of Glucocerebrosidase

(2012) Samarjit Patnaik et al.   JOURNAL OF MEDICINAL CHEMISTRY

Identification of HIV-1 Inhibitors Targeting the Nucleocapsid Protein

(2012) Sebastian Breuer et al.   JOURNAL OF MEDICINAL CHEMISTRY

High-Throughput Melting-Temperature Analysis of a Monoclonal Antibody by Differential Scanning Fluorimetry in the Presence of Surfactants

(2012) Tim Menzen et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

A FRET-based screening assay for nucleic acid ligands

(2012) Daniel Renčiuk et al.   METHODS

Protein Quality Control Acts on Folding Intermediates to Shape the Effects of Mutations on Organismal Fitness

(2012) Shimon Bershtein et al.   MOLECULAR CELL

Family-wide chemical profiling and structural analysis of PARP and tankyrase inhibitors

(2012) Elisabet Wahlberg et al.   NATURE BIOTECHNOLOGY

Differential Scanning Fluorometry Signatures as Indicators of Enzyme Inhibitor Mode of Action: Case Study of Glutathione S-Transferase

(2012) Wendy A. Lea et al.   PLoS One

Ternary complex structures of human farnesyl pyrophosphate synthase bound with a novel inhibitor and secondary ligands provide insights into the molecular details of the enzyme’s active site closure

(2012) Jaeok Park et al.   BMC STRUCTURAL BIOLOGY

Functional Rescue of F508del-CFTR Using Small Molecule Correctors

(2012) Steven Molinski et al.   Frontiers in Pharmacology

Thermal Denaturation Assays in Chemical Biology

(2011) Guillermo Senisterra et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Identification of a NBD1-Binding Pharmacological Chaperone that Corrects the Trafficking Defect of F508del-CFTR

(2011) Heidi M. Sampson et al.   CHEMISTRY & BIOLOGY

Structure and Mechanism of Human UDP-glucose 6-Dehydrogenase

(2011) Sigrid Egger et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Evaluation of Quinazoline Analogues as Glucocerebrosidase Inhibitors with Chaperone Activity

(2011) Juan J. Marugan et al.   JOURNAL OF MEDICINAL CHEMISTRY

High Throughput Formulation Screening for Global Aggregation Behaviors of Three Monoclonal Antibodies

(2011) Yi Li et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Comparison of fluorescence and light scattering based methods to assess formation and stability of protein–protein complexes

(2011) Jolanta Kopec et al.   JOURNAL OF STRUCTURAL BIOLOGY

Quantitation of protein-protein interactions by thermal stability shift analysis

(2011) Curtis J. Layton et al.   PROTEIN SCIENCE

High-throughput measurement, correlation analysis, and machine-learning predictions for pH and thermal stabilities of Pfizer-generated antibodies

(2011) Amy C. King et al.   PROTEIN SCIENCE

Calcium modulates force sensing by the von Willebrand factor A2 domain

(2011) Arjen J. Jakobi et al.   Nature Communications

A Molecular Mechanism for Bacterial Susceptibility to Zinc

(2011) Christopher A. McDevitt et al.   PLoS Pathogens

Higher throughput calorimetry: opportunities, approaches and challenges

(2010) Francisco E Torres et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Affinity-based screening techniques: their impact and benefit to increase the number of high quality leads

(2010) Christian Bergsdorf et al.   Expert Opinion on Drug Discovery

Formulation Development of Therapeutic Monoclonal Antibodies Using High-Throughput Fluorescence and Static Light Scattering Techniques: Role of Conformational and Colloidal Stability

(2010) Deborah S. Goldberg et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Biophysical characterization of recombinant proteins: A key to higher structural genomics success

(2010) Masoud Vedadi et al.   JOURNAL OF STRUCTURAL BIOLOGY

High-Affinity Inhibitors of Human NAD+-Dependent 15-Hydroxyprostaglandin Dehydrogenase: Mechanisms of Inhibition and Structure-Activity Relationships

(2010) Frank H. Niesen et al.   PLoS One

Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124

(2010) D. S. Auld et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Use of thermal melt curves to assess the quality of enzyme preparations

(2009) Gregory J. Crowther et al.   ANALYTICAL BIOCHEMISTRY

Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII

(2009) Asta Zubrienė et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Identification and Characterization of Ambroxol as an Enzyme Enhancement Agent for Gaucher Disease

(2009) Gustavo H. B. Maegawa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

High throughput thermostability screening of monoclonal antibody formulations

(2009) Feng He et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

High-Throughput Thermal Scanning: A General, Rapid Dye-Binding Thermal Shift Screen for Protein Engineering

(2009) Jason J. Lavinder et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression

(2009) D. S. Auld et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Specific Mechanism for Nonspecific Activation in Reporter-Gene Assays

(2008) Douglas S. Auld et al.   ACS Chemical Biology

ATLAS—A High-Throughput Affinity-Based Screening Technology for Soluble Proteins: Technology Application Using p38 MAP Kinase

(2008) Rupal Patel et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Identification of Ligand Binding by Protein Stabilization: Comparison of ATLAS with Biophysical and Enzymatic Methods

(2008) Peggy A. Thompson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Identification of Trinucleotide Repeat Ligands with a FRET Melting Assay

(2008) Samir Amrane et al.   CHEMBIOCHEM

High throughput methods of assessing protein stability and aggregation

(2008) Guillermo A. Senisterra et al.   Molecular BioSystems