Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biology
Wei Zou, Juanjuan Xing, Shijie Zou, Mei Jiang, Xinping Chen, Qi Chen, Daozheng Liu, Xiangcheng Zhang, Xin Fu
Summary: The study found that HIV-1 (LAI) Nef interferes with the development of HSPCs into myeloid-erythroid lineage cells, thus disrupting hematopoiesis. Therapeutics targeting Nef may hold promise in correcting HIV-1 associated hematopoietic abnormalities.
Article
Cell Biology
Fatemeh Safi, Parashar Dhapola, Sarah Warsi, Mikael Sommarin, Eva Erlandsson, Jonas Ungerback, Rebecca Warfvinge, Ewa Sitnicka, David Bryder, Charlotta Boiers, Ram Krishna Thakur, Goran Karlsson
Summary: By investigating the chromatin accessibility of single cells, it has been found that there is a population of cells that display both stem-like and lineage-affiliated chromatin signatures. These cells are positioned between stem cells and committed progenitors, showing multi-lineage potential but lacking self-renewal activity.
Article
Multidisciplinary Sciences
Leal Oburoglu, Els Mansell, Niels-Bjarne Woods
Summary: Glutamine metabolism plays an essential role in regulating the emergence and specification of hematopoietic cells during endothelial to hematopoietic transition (EHT), with different hematopoietic lineages requiring distinct derivatives of glutamine.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Fabrizio Martelli, Paola Verachi, Maria Zingariello, Maria Mazzarini, Alessandro M. Vannucchi, Annalisa Lonetti, Barbara Bacci, Giuseppe Sarli, Anna Rita Migliaccio
Summary: The study revealed that hGATA1 can rescue the anemia and thrombocytopenia caused by Gata1(low) mutation, but is ineffective in rescuing the megakaryocyte defect.
FRONTIERS IN GENETICS
(2021)
Article
Cell Biology
Zihan Xu, Libing He, Yilin Wu, Lu Yang, Cheng Li, Hong Wu
Summary: Loss of PTEN alters chromatin accessibility and leads to changes in lineage potentials of fetal liver HSCs, with PU.1 playing a critical role in modulating the epigenetic state and lineage plasticity of prepro-B progenitors.
Article
Engineering, Biomedical
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
Summary: This study demonstrates the successful generation of AGM-like hematopoietic system from human pluripotent stem cells through temporal manipulation of Wnt signaling. The generated hematopoietic progenitor cells resemble primary cord blood HSCs at the transcriptional level and exhibit lymphoid and myeloid potential in vitro. The cells also show the ability to home to a definitive hematopoietic site, rescue bloodless zebrafish, and engraft and repopulate in mouse recipients.
Article
Multidisciplinary Sciences
Irene Mariam Roy, P. V. Anu, Samantha Zaunz, Srinu Reddi, Aravind M. Giri, Rithika Saroj Sankar, Sarah Schouteden, Joerg Huelsken, Catherine M. Verfaillie, Satish Khurana
Summary: Interaction with microenvironmental factors is crucial for the regulation of hematopoietic stem cell function. In this study, it was found that integrin signaling regulates the expression of SDF-1 alpha through transcriptional regulation. Inhibition of SRC gene or pharmacological inhibition resulted in increased expression of the chemokine SDF-1 alpha. Systemic inhibition of SRC led to increased SDF-1 alpha levels in BM plasma, which improved the homing efficiency of transplanted HSCs and enhanced hematopoietic function.
Article
Hematology
Te Ling, Kevin Zhang, Jiayue Yang, Sandeep Gurbuxani, John D. Crispino
Summary: GATA1 mutations leading to the loss of N-terminal 83 amino acids are observed in various diseases, including myeloid leukemia in children with Down syndrome and certain types of anemia. A mouse model, expressing only the short GATA1 isoform, has been used to study hematopoiesis defects during gestation. However, this study demonstrates that Gata1s mutant mice display macrocytic anemia and abnormal megakaryopoiesis throughout their lifespan, suggesting the potential use of this model for GATA1 deficiency studies.
Article
Endocrinology & Metabolism
Josefine Tratwal, Shanti Rojas-Sutterlin, Charles Bataclan, Sabine Blum, Olaia Naveiras
Summary: Bone marrow adipocytes play important roles in the regulation of the hematopoietic microenvironment, with their heterogeneity determining their regulatory capacities in different contexts.
BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Review
Hematology
Nayoung Kwon, Evrett N. Thompson, Madeline Y. Mayday, Vanessa Scanlon, Yi-Chien Lu, Diane S. Krause
Summary: This review provides insights into our current understanding of fate decisions in bipotent megakaryocyte-erythroid progenitors (MEPs), discussing isolation of primary human MEP, gene expression patterns, transcription factors, and external factors involved in MEP fate determination, with an overview of debates in the field. Understanding MEP fate is crucial for studying diseases like anemia and leukemia, and MEP also serve as a model system for studying cell fate decisions, applicable to stem/progenitor cell biology.
CURRENT OPINION IN HEMATOLOGY
(2021)
Article
Cell & Tissue Engineering
Ece Somuncular, Julia Hauenstein, Prajakta Khalkar, Anne-Sofie Johansson, Ozge Dumral, Nicolai S. Frengen, Charlotte Gustafsson, Giuseppe Mocci, Tsu-Yi Su, Hugo Brouwer, Christine L. Trautmann, Michael Vanlandewijck, Stuart H. Orkin, Robert Mansson, Sidinh Luc
Summary: This study reveals the heterogeneity of hematopoietic stem cells (HSCs) and provides insights into the functional differences and regulatory mechanisms of HSCs through transcriptional and epigenetic mechanisms.
Article
Biochemistry & Molecular Biology
Meng-Xi Wang, Li Yan, Juan Chen, Jun-Mei Zhao, Jiang Zhu, Shan-He Yu
Summary: This study explored the feasibility of reducing the malignant characteristics of t(8;21) AML cells by reinforcing their endogenous differentiation potential. The results showed that in an AE9a murine model, the process of erythroid differentiation was accompanied by a decline or loss of leukemia-initiating potential. Therefore, enhancing the erythroid differentiation of t(8;21) AML cells may be a promising intervention strategy.
Review
Developmental Biology
Suwei Gao, Yifan Zhang, Feng Liu
Summary: For a long time, it was believed that self-renewing and multipotent hematopoietic stem cells (HSCs) were the main contributors to both embryonic and adult hematopoiesis. However, recent studies have shown that native hematopoiesis is not solely driven by hierarchical differentiation of HSCs, but rather by hematopoietic progenitor cells (HPCs). These findings help redefine our understanding of the cellular origins of embryonic and adult hematopoiesis and highlight the differences in revealing HSC potential versus HSC fate using different approaches.
Article
Hematology
Michiko Ichii, Kenji Oritani, Jun Toda, Hideaki Saito, Henyun Shi, Hirohiko Shibayama, Daisuke Motooka, Yuichi Kitai, Ryuta Muromoto, Jun-ichi Kashiwakura, Kodai Saitoh, Daisuke Okuzaki, Tadashi Matsuda, Yuzuru Kanakura
Summary: STAP-2 is an adaptor protein that plays a role in the growth and development of B cells. It is not essential under normal conditions, but its absence enhances the recovery of B cells following transplantation. Further research on STAP-2 may provide insights into speeding up the recovery of humoral immunity in response to chemotherapy or transplantation.
Article
Cardiac & Cardiovascular Systems
Ning Li, Thomas A. Csepe, Brian J. Hansen, Lidiya V. Sul, Anuradha Kalyanasundaram, Stanislav O. Zakharkin, Jichao Zhao, Avirup Guha, David R. Van Wagoner, Ahmet Kilic, Peter J. Mohler, Paul M. L. Janssen, Brandon J. Biesiadecki, John D. Hummel, Raul Weiss, Vadim V. Fedorov
Article
Cell & Tissue Engineering
J. Patrick Gonzalez, Sergii Kyrychenko, Viktoriia Kyrychenko, Joel S. Schneider, Celine J. Granier, Eric Himelman, Kevin C. Lahey, Qingshi Zhao, Ghassan Yehia, Yuan-Xiang Tao, Mantu Bhaumik, Natalia Shirokova, Diego Fraidenraich
Article
Cell Biology
Ning Li, Brian J. Hansen, Thomas A. Csepe, Jichao Zhao, Anthony J. Ignozzi, Lidiya V. Sul, Stanislav O. Zakharkin, Anuradha Kalyanasundaram, Jonathan P. Davis, Brandon J. Biesiadecki, Ahmet Kilic, Paul M. L. Janssen, Peter J. Mohler, Raul Weiss, John D. Hummel, Vadim V. Fedorov
SCIENCE TRANSLATIONAL MEDICINE
(2017)
Article
Developmental Biology
Celine Granier, Vasily Gurchenkov, Aitana Perea-Gomez, Anne Camus, Sascha Ott, Costis Papanayotou, Julian Iranzo, Anne Moreau, John Reid, Georgy Koentges, Delara Saberan-Djoneidi, Jerome Collignon
DEVELOPMENTAL BIOLOGY
(2011)
Article
Medicine, Research & Experimental
Natalia A. Kokorina, Stanislav O. Zakharkin, Paul H. Krebsbach, Brian Nussenbaum
Article
Medicine, Research & Experimental
Natalia A. Kokorina, James S. Lewis, Stanislav O. Zakharkin, Paul H. Krebsbach, Brian Nussenbaum
Article
Otorhinolaryngology
Jordan P. Sand, Natalia A. Kokorina, Stanislav O. Zakharkin, James S. Lewis, Brian Nussenbaum
OTOLARYNGOLOGY-HEAD AND NECK SURGERY
(2014)
Article
Cell & Tissue Engineering
Joseph Kramer, Celine J. Granier, Stephani Davis, Katherine Piso, Jane Hand, Arnold B. Rabson, Hatem E. Sabaawy
STEM CELLS AND DEVELOPMENT
(2013)
Article
Biology
Celine J. Granier, Wei Wang, Tiffany Tsang, Ruth Steward, Hatem E. Sabaawy, Mantu Bhaumik, Arnold B. Rabson
Article
Dermatology
Marion Godeau, Jason Shourick, Isabelle Dreyfus, Eline Casassa, Anais Bergeron, Maella Severino-Freire, Celine Granier Tournier, Julie Malloizel-Delaunay, Olivia Boccara, Helene Aubert, Annabel Maruani, Christine Chiaverini, Christine Labreze, Juliette Mazereeuw-Hautier
Summary: A validated HRQoL questionnaire for children aged 11-15 with LFMs was developed, showing excellent psychometric capacities and usability.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Letter
Dermatology
C. Valette, N. Jonca, J. Fischer, J. Pernin-Grandjean, C. Granier Tournier, A. Diociaiuti, I. Neri, I. Dreyfus, M. Furman, K. Giehl, A. Wollenberg, S. Mallet, L. Martin, A. Martin-Santiago, G. Onnis, P. Broue, S. Leclerc-Mercier, M. Schmuth, E. Sprecher, R. Gruber, K. Suessmuth, E. Bourrat, K. Komlosi, S. Hill, E. A. O'Toole, O. Schischmanoff, F. Caux, J. Mazereeuw-Hautier
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Virology
Christopher J. J. LaRocca, Kari L. L. Jacobsen, Kazuho Inoko, Stanislav O. O. Zakharkin, Masato Yamamoto, Julia Davydova
Summary: There have been significant advances in gene therapy and oncolytic virotherapy, particularly in the development of advanced animal models for testing these treatments. It is crucial to consider the biosafety risks when testing these vectors in animals. In this study, we conducted adenovirus-based viral shedding studies in murine models to determine the appropriate time to downgrade the animals from Biosafety Level 2 to Biosafety Level 1. Our results indicate that mice can be safely downgraded to BSL 1 after 72 hours following adenovirus injection, with no significant biologically active virus shedding.
Article
Cardiac & Cardiovascular Systems
Brian J. Hansen, Jichao Zhao, Ning Li, Alexander Zolotarev, Stanislav Zakharkin, Yufeng Wang, Josh Atwal, Anuradha Kalyanasundaram, Suhaib H. Abudulwahed, Katelynn M. Helfrich, Anna Bratasz, Kimerly A. Powell, Bryan Whitson, Peter J. Mohler, Paul Ml Janssen, Orlando P. Simonetti, John D. Hummel, Vadim V. Fedorov
JACC-CLINICAL ELECTROPHYSIOLOGY
(2018)