Biological and medical applications of a brain-on-a-chip

Food for thought ... on animal tests

(2016) Thomas Hartung   ALTEX-Alternatives to Animal Experimentation

Evidence-based toxicology – the toolbox of validation for the 21st century?

(2016) Thomas Hartung   ALTEX-Alternatives to Animal Experimentation

C-Type Natriuretic Peptide Modulates Permeability of the Blood–Brain Barrier

(2014) Manoj Bohara et al.   JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM

Instruction of Circulating Endothelial Progenitors In Vitro towards Specialized Blood-Brain Barrier and Arterial Phenotypes

(2014) Julie Boyer-Di Ponio et al.   PLoS One

Alternative approaches for medical countermeasures to biological and chemical terrorism and warfare

(2014) Thomas Hartung   ALTEX-Alternatives to Animal Experimentation

Look back in anger – what clinical studies tell us about preclinical work

(2014) Thomas Hartung   ALTEX-Alternatives to Animal Experimentation

Food for Thought … Mechanistic Validation

(2014) Thomas Hartung   ALTEX-Alternatives to Animal Experimentation

Dravet syndrome patient-derived neurons suggest a novel epilepsy mechanism

(2013) Yu Liu et al.   ANNALS OF NEUROLOGY

Evaluation of a human neurite growth assay as specific screen for developmental neurotoxicants

(2013) Anne K. Krug et al.   ARCHIVES OF TOXICOLOGY

Human Ntera2 cells as a predictive in vitro test system for developmental neurotoxicity

(2013) Michael Stern et al.   ARCHIVES OF TOXICOLOGY

A new dynamic in vitro modular capillaries-venules modular system: Cerebrovascular physiology in a box

(2013) Luca Cucullo et al.   BMC NEUROSCIENCE

Cerebral Organoids in a Dish: Progress and Prospects

(2013) Marina Bershteyn et al.   CELL

Human iPSC-Derived Oligodendrocyte Progenitor Cells Can Myelinate and Rescue a Mouse Model of Congenital Hypomyelination

(2013) Su Wang et al.   Cell Stem Cell

Modeling Dravet syndrome using induced pluripotent stem cells (iPSCs) and directly converted neurons

(2013) Jiao Jiao et al.   HUMAN MOLECULAR GENETICS

Investigation of Rett syndrome using pluripotent stem cells

(2013) Rana Dajani et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Fluorescent probes as a tool for cell population tracking in spontaneously active neural networks derived from human pluripotent stem cells

(2013) M. Mäkinen et al.   JOURNAL OF NEUROSCIENCE METHODS

Engineering of functional, perfusable 3D microvascular networks on a chip

(2013) Sudong Kim et al.   LAB ON A CHIP

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Timothy syndrome is associated with activity-dependent dendritic retraction in rodent and human neurons

(2013) Jocelyn F Krey et al.   NATURE NEUROSCIENCE

Scientific and ethical issues related to stem cell research and interventions in neurodegenerative disorders of the brain

(2013) Roger A. Barker et al.   PROGRESS IN NEUROBIOLOGY

Brief report: A human induced pluripotent stem cell model of cernunnos deficiency reveals an important role for XLF in the survival of the primitive hematopoietic progenitors

(2013) Katarzyna Tilgner et al.   STEM CELLS

Toward a 3D model of human brain development for studying gene/environment interactions

(2013) Helena T Hogberg et al.   Stem Cell Research & Therapy

Thick collagen-based 3D matrices including growth factors to induce neurite outgrowth

(2012) M.-N. Labour et al.   Acta Biomaterialia

Influence of a three-dimensional, microarray environment on human Cell culture in drug screening systems

(2012) Luciana Meli et al.   BIOMATERIALS

Reliable permeability assay system in a microfluidic device mimicking cerebral vasculatures

(2012) Ju Hun Yeon et al.   BIOMEDICAL MICRODEVICES

BBB ON CHIP: microfluidic platform to mechanically and biochemically modulate blood-brain barrier function

(2012) L. M. Griep et al.   BIOMEDICAL MICRODEVICES

Confined 3D microenvironment regulates early differentiation in human pluripotent stem cells

(2012) Giovanni G. Giobbe et al.   BIOTECHNOLOGY AND BIOENGINEERING

Induced Pluripotent Stem Cells from Patients with Huntington's Disease Show CAG-Repeat-Expansion-Associated Phenotypes

(2012) The HD iPSC Consortium   Cell Stem Cell

Progress made in the reprogramming field: new factors, new strategies and a new outlook

(2012) Samer MI Hussein et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Characterization of a microfluidic in vitro model of the blood-brain barrier (μBBB)

(2012) Ross Booth et al.   LAB ON A CHIP

SyM-BBB: a microfluidic blood brain barrier model

(2012) Balabhaskar Prabhakarpandian et al.   LAB ON A CHIP

A modular approach to create a neurovascular unit-on-a-chip

(2012) Anil Kumar H. Achyuta et al.   LAB ON A CHIP

Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells

(2012) Ethan S Lippmann et al.   NATURE BIOTECHNOLOGY

Modeling human cortical development in vitro using induced pluripotent stem cells

(2012) J. Mariani et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nanostructured Superhydrophobic Substrates Trigger the Development of 3D Neuronal Networks

(2012) Tania Limongi et al.   Small

Automated neurosphere sorting and plating by the COPAS large particle sorter is a suitable method for high-throughput 3D in vitro applications

(2012) K. Gassmann et al.   TOXICOLOGY IN VITRO

Astrocytes generated from patient induced pluripotent stem cells recapitulate features of Huntington’s disease patient cells

(2012) Tarja A Juopperi et al.   Molecular Brain

Establishment of hepatic and neural differentiation platforms of Wilson’s disease specific induced pluripotent stem cells

(2012) Fei Yi et al.   Protein & Cell

Human Neural Crest Stem Cells Derived from Human ESCs and Induced Pluripotent Stem Cells: Induction, Maintenance, and Differentiation into Functional Schwann Cells

(2012) Qiuyue Liu et al.   Stem Cells Translational Medicine

Validation and quality control of replacement alternatives – current status and future challenges

(2012) Marcel Leist et al.   Toxicology Research

Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells

(2011) Shin-ichiro Ogawa et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

Development of a three-dimensional, all-human in vitro model of the blood–brain barrier using mono-, co-, and tri-cultivation Transwell models

(2011) Kathryn Hatherell et al.   JOURNAL OF NEUROSCIENCE METHODS

Modelling schizophrenia using human induced pluripotent stem cells

(2011) Kristen J. Brennand et al.   NATURE

Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells

(2011) Ryan Lister et al.   NATURE

Using iPSC-derived neurons to uncover cellular phenotypes associated with Timothy syndrome

(2011) Sergiu P Paşca et al.   NATURE MEDICINE

Isogenic Pairs of Wild Type and Mutant Induced Pluripotent Stem Cell (iPSC) Lines from Rett Syndrome Patients as In Vitro Disease Model

(2011) Gene Ananiev et al.   PLoS One

Epigenetic Characterization of the FMR1 Gene and Aberrant Neurodevelopment in Human Induced Pluripotent Stem Cell Models of Fragile X Syndrome

(2011) Steven D. Sheridan et al.   PLoS One

Brief Report: Phenotypic Rescue of Induced Pluripotent Stem Cell-Derived Motoneurons of a Spinal Muscular Atrophy Patient

(2011) Tammy Chang et al.   STEM CELLS

Concise Review: Induced Pluripotent Stem Cells Versus Embryonic Stem Cells: Close Enough or Yet Too Far Apart?

(2011) Josipa Bilic et al.   STEM CELLS

Ochratoxin A at nanomolar concentration perturbs the homeostasis of neural stem cells in highly differentiated but not in immature three-dimensional brain cell cultures

(2011) Marie-Gabrielle Zurich et al.   TOXICOLOGY LETTERS

Concurrent imaging of synaptic vesicle recycling and calcium dynamics

(2011) Haiyan Li   Frontiers in Molecular Neuroscience

Prenatal Organochlorine Exposure and Behaviors Associated With Attention Deficit Hyperactivity Disorder in School-Aged Children

(2010) S. K. Sagiv et al.   AMERICAN JOURNAL OF EPIDEMIOLOGY

Increased Risk of ADHD Associated With Early Exposure to Pesticides, PCBs

(2010) Bridget M. Kuehn   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Astrocytes and Pericytes Differentially Modulate Blood—Brain Barrier Characteristics during Development and Hypoxic Insult

(2010) Abraham Al Ahmad et al.   JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM

Lessons Learned from Alternative Methods and their Validation for a New Toxicology in the 21st Century

(2010) Thomas Hartung   JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART B-CRITICAL REVIEWS

Pericytes are required for blood–brain barrier integrity during embryogenesis

(2010) Richard Daneman et al.   NATURE

Microelectrode arrays: A physiologically based neurotoxicity testing platform for the 21st century☆

(2010) Andrew F.M. Johnstone et al.   NEUROTOXICOLOGY

Application of micro-electrode arrays (MEAs) as an emerging technology for developmental neurotoxicity: Evaluation of domoic acid-induced effects in primary cultures of rat cortical neurons

(2010) Helena T. Hogberg et al.   NEUROTOXICOLOGY

Use of high content image analysis to detect chemical-induced changes in synaptogenesis in vitro

(2010) Joshua A. Harrill et al.   TOXICOLOGY IN VITRO

Ethanol inhibition of aspartyl-asparaginyl-β-hydroxylase in fetal alcohol spectrum disorder: Potential link to the impairments in central nervous system neuronal migration

(2009) Suzanne M. de la Monte et al.   ALCOHOL

Integrating human stem cell expansion and neuronal differentiation in bioreactors

(2009) Margarida Serra et al.   BMC BIOTECHNOLOGY

Vascular Mechanobiology

(2009) Joji Ando et al.   CIRCULATION JOURNAL

Human Neurospheres as Three-Dimensional Cellular Systems for Developmental Neurotoxicity Testing

(2009) Michaela Moors et al.   ENVIRONMENTAL HEALTH PERSPECTIVES

Toxicology for the twenty-first century

(2009) Thomas Hartung   NATURE

Differentiation of spinal motor neurons from pluripotent human stem cells

(2009) Bao-Yang Hu et al.   Nature Protocols

Structure and function of the blood–brain barrier

(2009) N. Joan Abbott et al.   NEUROBIOLOGY OF DISEASE

In vitro developmental neurotoxicity (DNT) testing: Relevant models and endpoints

(2009) Anna K. Bal-Price et al.   NEUROTOXICOLOGY

Comparison of PC12 and cerebellar granule cell cultures for evaluating neurite outgrowth using high content analysis

(2009) Nicholas M. Radio et al.   NEUROTOXICOLOGY AND TERATOLOGY

A Novel Combination of Factors, Termed SPIE, which Promotes Dopaminergic Neuron Differentiation from Human Embryonic Stem Cells

(2009) Tandis Vazin et al.   PLoS One

Generation of Functional Neural Artificial Tissue from Human Umbilical Cord Blood Stem Cells

(2009) Marcin Jurga et al.   TISSUE ENGINEERING PART C-METHODS

mRNA Expression is a Relevant Tool to Identify Developmental Neurotoxicants Using an In Vitro Approach

(2009) Helena T. Hogberg et al.   TOXICOLOGICAL SCIENCES

Neuronal in vitro models for the estimation of acute systemic toxicity

(2009) A. Forsby et al.   TOXICOLOGY IN VITRO

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell

Neuron network activity scales exponentially with synapse density

(2008) G J Brewer et al.   Journal of Neural Engineering

An intrinsic mechanism of corticogenesis from embryonic stem cells

(2008) Nicolas Gaspard et al.   NATURE

Relevance of in vitro neurotoxicity testing for regulatory requirements: Challenges to be considered

(2008) Anna K. Bal-Price et al.   NEUROTOXICOLOGY AND TERATOLOGY

Micropatterning of human embryonic stem cells dissects the mesoderm and endoderm lineages

(2008) Lawrence Haoran Lee et al.   Stem Cell Research

Gene expression as a sensitive endpoint to evaluate cell differentiation and maturation of the developing central nervous system in primary cultures of rat cerebellar granule cells (CGCs) exposed to pesticides

(2008) Helena T. Hogberg et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

A novel in vitro metabolomics approach for neurotoxicity testing, proof of principle for methyl mercury chloride and caffeine

(2007) Erwin van Vliet et al.   NEUROTOXICOLOGY