Article
Entomology
Basman H. Al-Jalely, Wei Xu
Summary: The study focused on the olfactory system of Trichogramma pretiosum, identifying 22 putative odorant binding proteins (OBPs) and 105 odorant receptors (ORs) using scanning electron microscopy to observe four types of olfactory sensilla. By examining the expression patterns of OBPs in male and female adults, three female-specific OBPs were identified, suggesting their crucial roles in host-seeking and oviposition behaviors. This research enriches our understanding of T. pretiosum olfactory genes and enhances our knowledge of its olfactory system.
Article
Entomology
Basman H. Al-Jalely, Penghao Wang, Yalin Liao, Wei Xu
Summary: This study conducted transcriptome sequencing on the antennae of male and female adult Diadegma semiclausum wasps, revealing differential expression of OBPs and ORs, providing important insights into the olfactory system of D. semiclausum.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2022)
Article
Entomology
Ningna Yin, Haiyan Xiao, Anjin Yang, Chun Wu, Naiyong Liu
Summary: This study investigates the correlation between host plant range and chemoreceptor gene repertoire sizes in Papilio butterflies. The results show that the breath of host plants does not appear to result in the obvious expansions of the odorant (ORs) and gustatory (GRs) receptors in Papilio butterflies. Additionally, candidate molecular targets associated with olfaction, oviposition, or reproduction in P. xuthus are identified.
Article
Biochemistry & Molecular Biology
Patrick Marcinek, Franziska Haag, Christiane Geithe, Dietmar Krautwurst
Summary: Molecular recognition plays a key role in biological systems, and the study of pyrazines has led to the discovery of OR5K1 as a specialized olfactory receptor across mammals for the detection of pyrazine-based key food odors and semiochemicals, indicating the significance of ecological evolution.
Article
Entomology
Jingtao Liu, Jiaoxin Xie, Adel Khashaveh, Jingjiang Zhou, Yongjun Zhang, Hui Dong, Bin Cong, Shaohua Gu
Summary: In this study, odorant receptor genes of the green peach aphid were identified and their phylogenetic relationship and genomic structure were analyzed. The results showed that the odorant receptors of the green peach aphid play an important role in chemical sensing and provide a foundation for investigating the interactions between aphids and hosts and natural enemies.
Article
Neurosciences
Ghazia Abbas, Spencer Tang, Joyce Noble, Robert P. Lane
Summary: The study found that odorant receptor coding sequences have intrinsic self-silencing capability, which may facilitate mutually exclusive odorant receptor expression in olfactory sensory neurons.
MOLECULAR AND CELLULAR NEUROSCIENCE
(2021)
Article
Chemistry, Applied
Weihong Liu, Yu Zheng, Chen Zhang, Lin Chen, Hanyi Zhuang, Guojun Yao, Hang Ren, Yingjian Liu
Summary: In this study, a biomimetic olfactory recognition system was developed to discriminate the aromas of Chinese liquors. By combining odorant receptors and machine learning, the system demonstrated high accuracy in differentiating liquors of different aroma types, brands, and ageing years. The interactions between liquor aroma compounds and odorant receptors were also elucidated, providing insights into odorant coding at the molecular level.
Article
Cell Biology
Mayra L. Ruiz Tejada Segura, Eman Abou Moussa, Elisa Garabello, Thiago S. Nakahara, Melanie Makhlouf, Lisa S. Mathew, Li Wang, Filippo Valle, Susie S. Y. Huang, Joel D. Mainland, Michele Caselle, Matteo Osella, Stephan Lorenz, Johannes Reisert, Darren W. Logan, Bettina Malnic, Antonio Scialdone, Luis R. Saraiva
Summary: This study created a genome-wide 3D atlas of the mouse olfactory mucosa using spatial transcriptomics. The distribution of odorant receptor genes (Olfrs) and non-Olfrs in different regions was found to be continuous and overlapping. The spatial locations of Olfrs correlated with the solubility of the odorants they recognized, providing evidence for the chromatographic theory of olfaction.
Article
Multidisciplinary Sciences
Enrico Lanza, Martina Di Rocco, Silvia Schwartz, Davide Caprini, Edoardo Milanetti, Giuseppe Ferrarese, Maria Teresa Lonardo, Luca Pannone, Giancarlo Ruocco, Simone Martinelli, Viola Folli
Summary: Chemosensory receptors are crucial for organisms like the nematode Caenorhabditis elegans to accurately distinguish a wide range of volatile/soluble molecules. Through behavioral assays, it was demonstrated that the AWC sensory neurons play a significant role in cancer odorants detection, enhancing discrimination accuracy. Additionally, chemotaxis assays on animals lacking GPCRs expressed in AWC helped identify receptors involved in binding cancer metabolites, highlighting the importance of these receptors in the cancer discriminating behavior of C. elegans.
SCIENTIFIC REPORTS
(2021)
Article
Entomology
Joerg Fleischer, Alexander Rausch, Kathrin Dietze, Silvio Erler, Sina Cassau, Juergen Krieger
Summary: The study explored the differences in expression of male-biased olfactory receptors in the antennae of European honey bees. It was found that these receptors are expressed in higher numbers in drone bees compared to female workers and queens, indicating a sex-specific difference in antennal cell expression. Further analyses revealed that male-biased receptors are expressed in distinct subsets of olfactory sensory neurons in drones, reminiscent of similar patterns seen in moths for detecting female-released sex pheromones.
Article
Cell Biology
L. Gonzalez-Brusi, J. G. Hamze, I Lamas-Toranzo, M. Jimenez-Movilla, P. Bermejo-Alvarez
Summary: This study aimed to identify sperm proteins interacting with the TMEM95 protein, which is essential for fertilization. Mass spectrometry identified 41 sperm proteins pulled down with TMEM95, suggesting an independent role of TMEM95 in fertilization. Among these proteins, OLFR601 is located in the acrosomal region and may mediate affinity for an odorant involved in fertilization. However, disruption of OLFR601 did not impair sperm fertilization ability, suggesting redundant function with other sperm proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katherine H. Shaw, Craig Dent, Travis K. Johnson, Alisha Anderson, Marien de Bruyne, Coral G. Warr
Summary: Research shows that olfactory behaviors in fruit flies are regulated by a large odorant receptor gene family, with variant alleles at the Or22 locus affecting the response properties of ab3A neurons and leading to changes in odorant preference and oviposition site preference in fruit flies.
Article
Agronomy
Xueqin Pan, Jun Liu, Xiao Xu, Liwei Zhang, Long Zhang
Summary: Choosing palatable plants is crucial for insect herbivores' survival, especially for restricted-feeders that specialize on few types of plants. However, the definitive strategy and sensory basis for distinguishing between host and nonhost plants remains controversial. In this study, we investigated the olfactory recognition of host plants in migratory locusts that feed on a limited range of plants. By using a novel behavioral paradigm, we found that the palps of locusts are necessary for differentiating between host and nonhost plants. The characteristic odors of the plants determined the behavioral differentiation, and intact palps were required for this process. We also identified the odorant receptors in the palps that modulate the recognition of key volatiles from host and nonhost plants, demonstrating the role of olfactory signaling in food choice.
Article
Biochemistry & Molecular Biology
Xiao-Qing Hou, Jothi Kumar Yuvaraj, Rebecca E. Roberts, Dan-Dan Zhang, C. Rikard Unelius, Christer Lofstedt, Martin N. Andersson
Summary: This study investigated the functional evolution of odorant receptors (ORs) in the Eurasian spruce bark beetle Ips typographus, revealing that these receptors primarily respond to compounds from host trees and beetle-associated fungi. The ORs showed a shared property of responding to monoterpenoids, indicating early neofunctionalization following gene duplication in this lineage.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Siddhant Kalra, Aayushi Mittal, Manisha Bajoria, Tripti Mishra, Sidrah Maryam, Debarka Sengupta, Gaurav Ahuja
Summary: High-throughput omics technologies have revealed the expression of olfactory receptors in nonolfactory tissues, but lack information about their cell type of origin. Single-cell RNA-sequencing technology addresses this issue and provides a new means for cell type-specific analysis, while large-scale single-cell expression atlases offer resources for systematic reannotation of extranasal olfactory receptors.
