Article
Anatomy & Morphology
Chan Wang, Bing Wang, Guirong Wang
Summary: This study investigated the sex pheromone recognition mechanism in the peripheral nervous system of Mythimna separata through electroantennogram recordings and single sensillum recording, revealing different types of trichoid sensilla with specific tuned neurons.
FRONTIERS IN NEUROANATOMY
(2021)
Article
Biochemistry & Molecular Biology
Sai Zhang, Shuwei Yan, Zhixiang Zhang, Song Cao, Bin Li, Yang Liu, Guirong Wang
Summary: This study identified the odorant receptor repertoire in three mirid bug species and confirmed that the candidate pheromone receptors have robust responses to main sex pheromone components. It uncovered the mechanism of pheromone perception in these species and provided a theoretical basis for screening effective attractants or mating disturbance agents for enhanced control of these destructive pests.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Katherine H. Shaw, Craig Dent, Travis K. Johnson, Alisha Anderson, Marien de Bruyne, Coral G. Warr
Summary: Research shows that olfactory behaviors in fruit flies are regulated by a large odorant receptor gene family, with variant alleles at the Or22 locus affecting the response properties of ab3A neurons and leading to changes in odorant preference and oviposition site preference in fruit flies.
Article
Biology
Jothi K. Yuvaraj, Rebecca E. Roberts, Yonathan Sonntag, Xiao-Qing Hou, Ewald Grosse-Wilde, Ales Machara, Dan-Dan Zhang, Bill S. Hansson, Urban Johanson, Christer Lofstedt, Martin N. Andersson
Summary: This study annotated 73 ORs from an antennal transcriptome of I. typographus and reported the functional characterization of two ORs (ItypOR46 and ItypOR49) responsive to single enantiomers of common bark beetle pheromone compounds. Homology modeling and molecular docking were used to predict their binding sites, providing insight into ligand-OR interaction for the functional evolution of the insect OR gene family. The ecological importance of these receptors and their potential use in pest control and biosensors to detect bark beetle infestations suggest further evaluation of these ORs.
Article
Agronomy
Xueqin Pan, Jun Liu, Xiao Xu, Liwei Zhang, Long Zhang
Summary: Choosing palatable plants is crucial for insect herbivores' survival, especially for restricted-feeders that specialize on few types of plants. However, the definitive strategy and sensory basis for distinguishing between host and nonhost plants remains controversial. In this study, we investigated the olfactory recognition of host plants in migratory locusts that feed on a limited range of plants. By using a novel behavioral paradigm, we found that the palps of locusts are necessary for differentiating between host and nonhost plants. The characteristic odors of the plants determined the behavioral differentiation, and intact palps were required for this process. We also identified the odorant receptors in the palps that modulate the recognition of key volatiles from host and nonhost plants, demonstrating the role of olfactory signaling in food choice.
Article
Agriculture, Multidisciplinary
Qiu Tang Huang, Cheng Wang Sheng, Andrew K. Jones, Jie Jiang, Tao Tang, Zhao Jun Han, Chun Qing Zhao
Summary: Cs8916 is a potential subunit of the iGABA receptor, which can interact with CsLCCH3 to form a cation-selective channel sensitive to GABA or beta-alanine. It is also sensitive to certain insecticides, such as dieldrin, endosulfan, fipronil, and ethiprole, while showing little effect with others like fluralaner, broflanilide, and avermectin. Additionally, Cs8916/RDL1 has different responses to GABA and insecticides compared to homomeric CsRDL1, indicating a potential role in fine-tuning GABAergic signaling.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Physiology
Tianliang Ji, Zhi Xu, Qingchen Jia, Guirong Wang, Youming Hou
Summary: This study identified an antenna-biased expressed olfactory receptor RferOr6 used by the red palm weevil to detect the volatile compound alpha-pinene in non-palm plants. Results showed that alpha-pinene can significantly repel the red palm weevil, providing a foundation for further research on the functional studies of Or6 in the pest.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Patrick Marcinek, Franziska Haag, Christiane Geithe, Dietmar Krautwurst
Summary: Molecular recognition plays a key role in biological systems, and the study of pyrazines has led to the discovery of OR5K1 as a specialized olfactory receptor across mammals for the detection of pyrazine-based key food odors and semiochemicals, indicating the significance of ecological evolution.
Article
Neurosciences
Rebecca E. Roberts, Jothi Kumar Yuvaraj, Martin N. Andersson
Summary: This study explored the potential of codon optimization for insect odorant receptor genes to enhance their expression and functional characterization in human cells, particularly HEK293 cells. While codon optimization improved protein levels and functional characterization for some odorant receptors, it was not universally effective for all receptors tested, suggesting limitations in this approach for studying insect ORs in HEK cells.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Parasitology
Ru Yan, Zhanyi Xu, Jiali Qian, Qiaoling Zhou, Huiming Wu, Yahui Liu, Yirong Guo, Guonian Zhu, Mengli Chen
Summary: This study investigates the olfactory system of the Asian tiger mosquito Aedes albopictus and reveals that AalbOr11 is a broadly tuned receptor. Furthermore, AalbOr11 may mediate the repellency activity of (+)-fenchone. This research helps to deepen our understanding of the mosquito's olfactory system and provides potential semiochemicals for monitoring and controlling mosquito populations.
PARASITES & VECTORS
(2022)
Article
Chemistry, Applied
Weihong Liu, Yu Zheng, Chen Zhang, Lin Chen, Hanyi Zhuang, Guojun Yao, Hang Ren, Yingjian Liu
Summary: In this study, a biomimetic olfactory recognition system was developed to discriminate the aromas of Chinese liquors. By combining odorant receptors and machine learning, the system demonstrated high accuracy in differentiating liquors of different aroma types, brands, and ageing years. The interactions between liquor aroma compounds and odorant receptors were also elucidated, providing insights into odorant coding at the molecular level.
Article
Entomology
Basman H. Al-Jalely, Penghao Wang, Yalin Liao, Wei Xu
Summary: This study conducted transcriptome sequencing on the antennae of male and female adult Diadegma semiclausum wasps, revealing differential expression of OBPs and ORs, providing important insights into the olfactory system of D. semiclausum.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2022)
Article
Entomology
Basman H. Al-Jalely, Wei Xu
Summary: The study focused on the olfactory system of Trichogramma pretiosum, identifying 22 putative odorant binding proteins (OBPs) and 105 odorant receptors (ORs) using scanning electron microscopy to observe four types of olfactory sensilla. By examining the expression patterns of OBPs in male and female adults, three female-specific OBPs were identified, suggesting their crucial roles in host-seeking and oviposition behaviors. This research enriches our understanding of T. pretiosum olfactory genes and enhances our knowledge of its olfactory system.
Article
Biochemistry & Molecular Biology
R. Jason Pitts, Robert M. Huff, Shan Ju Shih, Jonathan D. Bohbot
Summary: The research identifies indolOR genes in mosquitoes, flies, and Culicidae, shedding light on insect chemical ecology and providing targets for novel odor-based tools.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Enrico Lanza, Martina Di Rocco, Silvia Schwartz, Davide Caprini, Edoardo Milanetti, Giuseppe Ferrarese, Maria Teresa Lonardo, Luca Pannone, Giancarlo Ruocco, Simone Martinelli, Viola Folli
Summary: Chemosensory receptors are crucial for organisms like the nematode Caenorhabditis elegans to accurately distinguish a wide range of volatile/soluble molecules. Through behavioral assays, it was demonstrated that the AWC sensory neurons play a significant role in cancer odorants detection, enhancing discrimination accuracy. Additionally, chemotaxis assays on animals lacking GPCRs expressed in AWC helped identify receptors involved in binding cancer metabolites, highlighting the importance of these receptors in the cancer discriminating behavior of C. elegans.
SCIENTIFIC REPORTS
(2021)