Article
Multidisciplinary Sciences
Mirko Marino, Letizia Gigliotti, Peter Moller, Patrizia Riso, Marisa Porrini, Cristian Del Bo
Summary: The comet assay is used to assess DNA damage in tissues and biological samples. This study found that storage time had varying effects on DNA damage levels in PBMCs and WB samples, with significant increases observed in WB samples stored for over 6 months. WB samples showed higher levels of DNA damage compared to PBMCs.
SCIENTIFIC REPORTS
(2021)
Article
Public, Environmental & Occupational Health
Martin Roursgaard, Monika Hezareh Rothmann, Juliane Schulte, Ioanna Karadimou, Elena Marinelli, Peter Moller
Summary: This study investigated the extraction of nanoplastic particles from food containers and found that these nanoparticles can cause genotoxicity in cell cultures, while their cytotoxic effects were limited.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Food Science & Technology
Merve Bacanli, Ozgur Esim, Hakan Erdogan, Meral Sarper, Onur Erdem, Yalcin Ozkan
Summary: This study evaluated the cytotoxicity and genotoxicity of paclitaxel nanoencapsulated with PLGA in human neuroblastoma cells. Results showed that PLGA nanoparticles can be considered as a biocompatible carrier system for drug delivery and might be a promising agent against neuroblastoma.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Review
Environmental Sciences
Aryelle Canedo, Thiago Lopes Rocha
Summary: Zebrafish have become a popular model for assessing the genotoxicity of pollutants due to their orthologous genes and involvement in repair pathways. Comet assay is the main technique used in these studies, but standardization is needed for better interpretation and comparisons. Understanding repair mechanisms in zebrafish subjected to DNA damage is essential for further research on genotoxic effects caused by pollutants.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Alaa El-Din H. Sayed, Shaimaa K. Idriss, Sary Kh. Abdel-Ghaffar, Asmaa A. A. Hussein
Summary: The study examined the DNA damage response in erythrocytes of fish exposed to volatile organic compounds (VOCs), finding evidence of genotoxic effects and potential biomarkers for environmental pollution. Despite the dangers of VOCs, little is known about their hemotoxic, cytotoxic, and genotoxic effects on fish. The research optimized an assay method to measure apoptosis and DNA damage in erythrocytes of tilapia fish exposed to benzene, toluene, and xylene and identified the highest levels of apoptosis, DNA damage, and histopathological alterations in fish exposed to benzene.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Marcelino Benvindo-Souza, Daiany Folador Sotero, Cirley Gomes Araujo dos Santos, Rhayane Alves de Assis, Rinneu Elias Borges, Lia Raquel de Souza Santos, Daniela de Melo e Silva
Summary: Pollution from mining activities can cause genotoxic damage to bats, particularly in species like Carollia perspicillata and Glossophaga soricina. High levels of elements such as Mn, Cr, Pb, and Zn were found in water samples from the mining area. The study suggests that bats in mining areas are susceptible to increased DNA damage, similar to what has been observed in other species.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Environmental Sciences
Felipe de Araujo Nascimento, Daniela de Melo e Silva, Thays Millena Alves Pedroso, Jheneffer Sonara Aguiar Ramos, Michelle Rocha Parise
Summary: Occupational exposure to pesticides in farmers results in significant DNA damage, showing a notable impact and variability in the studies. Smokers who are occupationally exposed to pesticides have slightly reduced DNA damage compared to non-smokers, indicating some genetic toxicity. The study demonstrates a higher risk of occupationally exposed rural workers developing related diseases due to the genotoxic effect of pesticides.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Environmental Sciences
Vehbi Atahan Togay, Gulcin Yavuz Turel, Dilek Asci celik, Meltem ozgocmen, Eda Evgen Tuluceoglu, Ismail Sen, Yusuf Ayvaz
Summary: This study found that cyprodinil and thiacloprid exposure can lead to increased DNA damage risk in zebrafish, as demonstrated by comet assay. These pesticides were identified as genotoxic agents that require further investigation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Peter Moller, Regitze Solling Wils, Emilio Di Ianni, Claudia Andrea Torero Gutierrez, Martin Roursgaard, Nicklas Raun Jacobsen
Summary: The review summarizes the genotoxic effects of JRC materials and MWCNT-7, with a significant increase in the current database compared to the IARC monograph. Cell culture studies show a genotoxic response by MWCNT-7, less consistent effect by NM-400 and NM-402, and least consistent effect by NM-401 and NM-403, indicating strongest evidence of genotoxicity for MWCNT-7. There are limited observations from animal models and humans to draw general conclusions about genotoxicity.
MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH
(2021)
Article
Environmental Sciences
Rafael Valencia-Quintana, Mirta Milic, Stefano Bonassi, Maria Antonieta Ochoa-Ocana, Victoria Campos-Pena, Maria Guadalupe Tenorio-Arvide, Guillermo Alejandro Perez-Flores, Juana Sanchez-Alarcon
Summary: This study evaluated the effect of pesticides on genetic damage to agricultural workers in Los Reyes, Michoacan, Mexico. The results showed that pesticide use poses a risk of genetic damage to agricultural workers, but measures can be taken to reduce this risk.
Article
Pharmacology & Pharmacy
Jorid B. Sorlia, Alexander C. O. Jensen, Alicja Mortensen, Jozef Szarek, Claudia A. T. Gutierrez, Lucas Givelet, Katrin Loeschner, Charis Loizides, Iosif Hafez, George Biskos, Ulla Vogel, Niels Hadrup
Summary: By conducting nose-only inhalation tests on mice, we found that the air concentration of molybdenum disulphide particles has no observed adverse effect on body weight gain and genotoxicity below 13mg/m³, while the effects on respiratory function suggest this level as the lowest observed adverse effect concentration.
Article
Environmental Sciences
Claudia Castro, Ana Carvalho, Isabel Gaivao, Jose Lima-Brito
Summary: The study evaluated the tolerance of four wine-producing varieties to different concentrations of Cu by treating V. vinifera cuttings with Cu-rich hydroponic solutions. It was found that 50μM Cu treatment increased DNA damage, with 'Tinto Cao' being the most sensitive variety. Comet-FISH analysis revealed partial damage on rDNA and telomeric DNA in response to Cu treatment.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Nanoscience & Nanotechnology
Regitze Solling Wils, Nicklas Raun Jacobsen, Emilio Di Ianni, Martin Roursgaard, Peter Moller
Summary: This study evaluated the genotoxicity of different types of carbon nanotubes on mouse lung epithelial cells, showing that CNTs can cause DNA damage, reactive oxygen species production, and impact telomere length in long-term exposure.
Article
Biochemistry & Molecular Biology
Lina Hudhud, David R. Chisholm, Andrew Whiting, Anita Steib, Krisztina Pohoczky, Angela Kecskes, Eva Szoke, Zsuzsanna Helyes
Summary: We investigated the effects of stable diphenylacetylene-based analogues of all-trans-retinoic acid (ATRA) on cell viability and genotoxicity. These analogues demonstrated variation in cytotoxic activity, with some showing stronger genotoxicity at higher concentrations. Although these compounds exhibited retinoid receptor-independent genotoxicity, further research is needed to understand their molecular mechanisms and complex biological functions.
Article
Environmental Sciences
Marcelino Benvindo-Souza, Akemi Vieira Hosokawa, Cirley Gomes Araujo dos Santos, Rhayane Alves de Assis, Thays Millena Alves Pedroso, Rinneu Elias Borges, Susi Missel Pacheco, Lia Raquel de Souza Santos, Daniela de Melo e Silva
Summary: Habitat loss and fragmentation pose significant threats to biodiversity. The use of bioindicators can provide insight into the health of the environment. A study conducted in agricultural areas found higher genotoxic damage in bats compared to a control area.
ENVIRONMENTAL POLLUTION
(2022)
Article
Entomology
Welber Costa Pina, Lenice Souza-Shibatta, Natalia Uemura, Andre Luiz Gobatto, Gabriele Antico Freiria, Silvia Helena Sofia
Summary: This study describes the mating behavior of Epicharis dejeanii and evaluates the reproductive strategies employed by males. The results show that males use different patrol strategies during mating, but both strategies are effective in copulation success. The study also reveals a correlation between patrol strategy and male body size.
JOURNAL OF APICULTURAL RESEARCH
(2022)
Article
Entomology
Denilson Costa Martins, Patricia Maia Correia de Albuquerque, Jose Manuel Macario Rebelo, Thais Kotelok-Diniz, Silvia Helena Sofia, Wilson Frantine-Silva
Summary: This study investigated the genetic diversity and population structure of the orchid bee species E. cordata in different Brazilian biomes. The results suggest that local factors and historical factors, rather than vegetation or geographic distances, drive the observed genetic structure. These findings have important implications for the conservation of orchid bees.
JOURNAL OF APICULTURAL RESEARCH
(2023)
Article
Oncology
T. S. Freire, M. P. Mori, J. N. F. A. Miranda, L. Y. M. Muta, F. T. Machado, N. C. Moreno, N. C. Souza-Pinto
Summary: XPC deficiency is linked to mitochondrial dysfunction and increased p53 expression, while treatment with antioxidants can help restore cellular balance.
Article
Biochemistry & Molecular Biology
Nathalia Quintero-Ruiz, Camila Corradi, Natalia Cestari Moreno, Tiago Antonio de Souza, Ligia Pereira Castro, Clarissa Ribeiro Reily Rocha, Carlos Frederico Martins Menck
Summary: Nucleotide excision repair (NER) is crucial for genome protection from UVB-induced mutations, as seen in XP-C-deficient cells which exhibit a mutation spectrum similar to human skin cancer.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2022)
Article
Zoology
Dhiego G. Ferreira, Bruno A. Galindo, Caroline Apolinario-Silva, Raul H. C. Nascimento, Wilson Frantine-Silva, Analiza F. Cavenagh, Matheus M. Silva, Daniele C. Feliciano, Carlos E. G. Aggio, Augusto S. Zanatta, Sandremir Carvalho, Silvia H. Sofia
Summary: Due to the increasing global demand for electricity, many countries have been seeking methods to diversify their power production. However, hydroelectric power plants still remain the predominant source in countries like Brazil, occupying important drainage systems. This study analyzed the genetic distribution of fish populations in the Upper Parana River basin by examining the DNA of non-migratory fish species upstream and downstream of three Small Hydroelectric Plants. The results indicate that Small Hydroelectric Plants have genetically subdivided fish populations, and other factors such as the number of plants and connectivity with undisturbed stretches of rivers can also influence genetic differentiation levels.
STUDIES ON NEOTROPICAL FAUNA AND ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Marcela T. Latancia, Natalia C. Moreno, Giovana S. Leandro, Victoria Chaves Ribeiro, Izadora de Souza, William Kleber Martins Vieira, Andre Uchimura Bastos, Nicolas Carlos Hoch, Clarissa R. R. Rocha, Carlos F. M. Menck
Summary: This study investigates the effects of TMZ treatment on human XP-V cells and its complemented counterpart. The results show that pol eta plays a role in protecting human cells from TMZ-induced DNA damage, but this role may be different from its canonical TLS mechanism. This finding opens up new therapeutic possibilities of using pol eta as a target to improve the efficacy of TMZ-based cancer therapies.
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
(2022)
Review
Entomology
Lucas Battisti, Michele Potrich, Everton Ricardi Lozano, Claudia Bueno dos Reis Martinez, Silvia Helena Sofia
Summary: Bees are declining worldwide, and pesticide use, including herbicides, has been found to negatively impact bees. Glyphosate (GLY) is the most sold pesticide and exposure to GLY can cause sublethal effects on bees. This study reviewed the literature on the sublethal effects of GLY and commercial formulations on bees. The findings showed that GLY exposure can alter gene expression, enzyme functioning, metabolism, tissue structure, microbiota diversity, learning, feeding, flight, circadian cycle, and body development in bees. Further research is needed to understand the long-term effects of GLY on bee colony homeostasis and the toxicity of surfactants in different commercial formulations.
JOURNAL OF APPLIED ENTOMOLOGY
(2023)
Article
Biodiversity Conservation
Andre Luiz Gobatto, Patricia Nakayama Miranda, Natalia Uemura, Susanna Mendes Miranda, Welber Costa Pina, Silvia Helena Sofia
Summary: This study evaluated the influence of agricultural landscapes on the abundance and diversity of trap-nesting bees, wasps, and their natural enemies in restored areas. The results showed that monoculture agriculture had a negative impact on the abundance of wasps and their natural enemies, while bee diversity was negatively related to the distance from the edge of reforestation to the nearest forest fragment. The findings suggest that the characteristics of the surrounding landscape should be considered in restoration projects to maximize the effectiveness of ecological services.
BIODIVERSITY AND CONSERVATION
(2023)
Article
Biochemistry & Molecular Biology
Larissa Costa de Almeida, Felipe Antunes Calil, Natalia Cestari Moreno, Paula Rezende-Teixeira, Luiz Alberto Beraldo de Moraes, Paula Christine Jimenez, Carlos Frederico Martins Menck, Joao Agostinho Machado-Neto, Leticia Veras Costa-Lotufo
Summary: DNA-targeting agents have clinical significance in anticancer chemotherapy, but their widespread use is limited due to toxicity. This study used in silico modeling and transcriptomics to characterize the DNA repair pathways activated by pradimicin-IRD treatment. Pradimicin-IRD was found to be a DNA intercalating agent and can potentially inhibit DNA-binding proteins. Genes related to DNA repair functions were modulated by pradimicin-IRD, providing insights for further research on this compound as a new antineoplastic agent.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Entomology
Wilson Frantine-Silva, Douglas Caldeira Giangarelli, Guaraci Duran Cordeiro, Isabel Alves-Dos-Santos, Maria Cristina Gaglianone, Silvia Helena Sofia
Summary: This study reveals that Pleistocene climate fluctuations have influenced the population structure of the orchid bee species, and the genetic structure aligns with the climatic models. Population expansion occurred after the Last Glacial Maximum. The genetic structure of Euglossa annectans in this area is reported for the first time.
JOURNAL OF APICULTURAL RESEARCH
(2023)
Meeting Abstract
Environmental Sciences
Natalia Cestari Moreno, Dana D'Orlando, Marcela T. Latancia, Irina Bezsonova, Nicholas W. Ashton, Roger Woodgate
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2023)
Meeting Abstract
Environmental Sciences
Marcela Teatin Latancia, Natalia Cestari Moreno, Giovana Leandro, Andre Uchimura Bastos, Davi Jardim, Izadora Souza, Clarissa R. R. Rocha, Carlos F. M. Menck
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2022)
Meeting Abstract
Environmental Sciences
Natalia C. Moreno, Tiago A. Souza, Camila C. M. Garcia, Clarissa R. R. Rocha, Veridiana Munford, Ligia P. Castro, Camila Corradi, Nathalia R. Quintero, Nadja C. Souza-Pinto, Carlos F. M. Menck
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2022)
Meeting Abstract
Environmental Sciences
Maria C. C. Ramalho, Isabeli Y. A. Osawa, Natalia C. Moreno, Marina Andrade-Tomaz, Hugo A. Armelin, Clarissa R. R. Rocha, Luciana R. Gomes
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
(2022)
Meeting Abstract
Oncology
Marcela Teatin Latancia, Andre Uchimura Bastos, Natalia C. Moreno, Davi Jardim, Clarissa R. R. Rocha, Giovana Leandro, Carlos Frederico Martins Menck
