Article
Multidisciplinary Sciences
Adrian Aguirre-Tamaral, Manuel Cambon, David Poyato, Juan Soler, Isabel Guerrero
Summary: Cell-cell communication is crucial during embryonic development, and cytonemes, filopodia-like structures, have been proposed as a mechanism for morphogen gradient formation. The stabilization and orientation of cytonemes may depend on the levels of Ihog and glypicans, suggesting a guiding mechanism.
NATURE COMMUNICATIONS
(2022)
Article
Biology
Ryo Hatori, Brent M. Wood, Guilherme Oliveira Barbosa, Thomas B. Kornberg
Summary: The study found that the dispersion of morphogen signaling proteins to their targets is regulated; most of Hh and some Wg are taken up by cells and activate signaling; the amount of morphogen taken up and initiating signaling does not change with varying expression levels.
Article
Chemistry, Physical
Haiyi Chen, Yu Kang, Mojie Duan, Tingjun Hou
Summary: The study revealed that the presence of heparan sulfate is crucial for the binding between the spike protein of SARS-CoV-2 and host ACE2, while the stability of the T470-F490 loop and hydrophobic interactions play key roles in the binding process.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Dominique Manikowski, Georg Steffes, Jurij Froese, Sebastian Exner, Kristina Ehring, Fabian Gude, Daniele Di Iorio, Seraphine V. Wegner, Kay Grobe
Summary: Morphogens play a crucial role in cell differentiation in a concentration dependent manner. In the case of Hedgehog (Hh) morphogens, they spread from their source to guide growth and pattern formation in the Drosophila wing disc. Heparan sulfate (HS) expression is essential for this process, and it has been found that HS directly regulates Hh signaling and spread in the wing disc epithelium. Interfering with this regulation alters Hh signaling range and gradient robustness.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Cell Biology
Toshihiro Inubushi, Nag Priyanka, Masakatsu Watanabe, Yusuke Takahashi, Shinnosuke Kusano, Hiroshi Kurosaka, Silvana Papagerakis, Petros Papagerakis, Mikako Hayashi, Takashi Yamashiro
Summary: This study demonstrates that the chlorinated oxidant MA-T can enhance dentinogenesis by modifying HSPG and activating Wnt signaling. The treatment decreases sulfation of HSPG, upregulates the expression of dentin-related genes, and promotes dentin formation in developing teeth.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Xin Luo, Nellie A. Campbell, Li He, Daniel R. O'Brien, Mark S. Singer, Hassan Lemjabbar-Alaoui, Keun Soo Ahn, Rory Smoot, Michael S. Torbenson, Steven D. Rosen, Lewis R. Roberts
Summary: The study reveals the important role of SULF2 in CCA, regulating the PDGFRβ-YAP signaling pathway to promote tumor growth and chemoresistance. Targeting SULF2 with the anti-SULF2 mouse monoclonal antibody 5D5 can inhibit the PDGFRβ-YAP signaling pathway and slow down tumor growth.
Article
Biology
Shu Yang, Ya Zhang, Chuxuan Yang, Xuefeng Wu, Sarah Maria El Oud, Rongfang Chen, Xudong Cai, Xufeng S. Wu, Ganhui Lan, Xiaoyan Zheng
Summary: The study reveals that Ihog in Drosophila not only forms a complex with Patched to mediate signaling, but also stabilizes cytonemes through trans-homophilic binding, both involving a specific heparin-binding site. The heterophilic Hh-Ihog interaction dominates, displacing Ihog molecules involved in trans-homophilic binding along cytonemes. The seemingly conflicting functions of Ihog in homophilic adhesion and ligand binding cooperate to facilitate Hh transport and reception along cytonemes.
Article
Biochemistry & Molecular Biology
Linh Nguyen, Kelli A. McCord, Duong T. Bui, Kim M. Bouwman, Elena N. Kitova, Mohamed Elaish, Dhanraj Kumawat, Gour C. Daskhan, Ilhan Tomris, Ling Han, Pradeep Chopra, Tzu-Jing Yang, Steven D. Willows, Andrew L. Mason, Lara K. Mahal, Todd L. Lowary, Lori J. West, Shang-Te Danny Hsu, Tom Hobman, Stephen M. Tompkins, Geert-Jan Boons, Robert P. de Vries, Matthew S. Macauley, John S. Klassen
Summary: Evidence suggests that host glycans, specifically glycolipids containing sialic acid, play a role in facilitating the entry of SARS-CoV-2 virus into host cells by binding to the receptor-binding domain (RBD) of the spike protein. Depletion of cell surface sialic acid levels through various methods decreases RBD binding and infection of the virus, indicating the importance of sialylated glycans in viral entry.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Cell Biology
Olivia Spead, Trevor Moreland, Cory J. Weaver, Irene Dalla Costa, Brianna Hegarty, Kenneth L. Kramer, Fabienne E. Poulain
Summary: This study reveals the important roles of Glypican-3, Teneurin-3, and Latrophilin-3 in regulating axonal pruning in the visual system through trans-axonal signaling. Glypican-3 and Teneurin-3 are selectively expressed by ventral retinal ganglion cells and cooperate to correct missorted dorsal axons, while the adhesion G-protein-coupled receptor Latrophilin-3 signals along dorsal axons to initiate the elimination of topographic sorting errors.
Article
Cell Biology
Charlotte Mallart, Fabienne Chalvet, Sophie Netter, Alba Yurani Torres, Mickael Poidevin, Jacques Montagne, Anne-Marie Pret, Marianne Malartre
Summary: The JAK-STAT pathway plays an important role in Drosophila, but its regulation mechanism is not fully understood. This study discovered that JAK-STAT signaling displays dynamic asymmetry during Drosophila oogenesis, similar to the accumulation of the adherens junction protein E-cadherin. The study also revealed the critical role of E-cadherin in regulating JAK-STAT activity throughout oogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Valeria De Pasquale, Miriam Shasa Quiccione, Simona Tafuri, Luigi Avallone, Luigi Michele Pavone
Summary: Heparan sulfate proteoglycans (HSPGs) are a group of glycoproteins with diverse structures, capable of interacting with various ligands to regulate cellular processes; they also assist many viruses in invading host cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Anett Hudak, Matthew Roach, David Pusztai, Aladar Pettko-Szandtner, Annamaria Letoha, Laszlo Szilak, Mimoun Azzouz, Tamas Letoha
Summary: AAV vectors are safe and efficient gene delivery tools, with AAV9 being capable of crossing the blood-brain barrier for gene transduction in the central nervous system. However, recent studies have revealed limitations in AAV9-mediated gene delivery. This study identified syndecan-4 as a significant contributor to AAV9's cellular internalization, highlighting its role in enhancing gene transduction. By understanding the molecular mechanisms involved, this research provides insights to improve AAV9-based gene therapy approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Juliana Pocas, Catarina Marques, Catarina Gomes, Andreia Hanada Otake, Filipe Pinto, Mariana Ferreira, Tiago Silva, Isabel Faria-Ramos, Rita Matos, Ana Raquel Ribeiro, Emanuel Senra, Bruno Cavadas, Silvia Batista, Joana Maia, Joana A. Macedo, Luis Lima, Luis Pedro Afonso, Jose Alexandre Ferreira, Lucio Lara Santos, Antonio Polonia, Hugo Osorio, Mattias Belting, Celso A. Reis, Bruno Costa-Silva, Ana Magalhaes
Summary: Gastric cancer is influenced by the expression of syndecan-4 (SDC4) which is associated with poor patient survival. SDC4 plays a crucial role in regulating the motility and invasion of gastric cancer cells. Additionally, SDC4 decorated with heparan sulfate is involved in the distribution, uptake, and functional effects of extracellular vesicles (EVs) derived from gastric cancer cells. SDC4 knockout disrupts the tropism of EVs for common gastric cancer metastatic sites. These findings provide insight into the molecular implications of SDC4 expression in gastric cancer cells and present potential therapeutic strategies targeting the glycan-EV axis to limit tumor progression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Isabel Faria-Ramos, Juliana Pocas, Catarina Marques, Joao Santos-Antunes, Guilherme Macedo, Celso A. Reis, Ana Magalhaes
Summary: Heparan sulfate (HS) plays a crucial role in cancer development, impacting various aspects such as proliferation, angiogenesis, and metastasis. Due to its interaction with different ligands, HS has pleiotropic effects on important cellular receptors and downstream signaling pathways. Aberrant expression of HS can determine malignant features of tumors.
Review
Biochemistry & Molecular Biology
Tanya Waseem, Madiha Ahmed, Tausif Ahmed Rajput, Mustafeez Mujtaba Babar
Summary: Diabetes mellitus leads to metabolic derangements and organ damage, partially due to glycosaminoglycan (GAG) damage in the basement membrane. GAGs are polysaccharides with repeating disaccharide units and play a significant role in cellular processes. The destruction of extracellular matrix can result in various diseases in organs. GAG-based therapeutic agents are being evaluated for managing diabetic complications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Masahiko Takemura, Hiroshi Nakato
JOURNAL OF CELL SCIENCE
(2017)
Article
Multidisciplinary Sciences
Rieko Imae, Katsufumi Dejima, Eriko Kage-Nakadai, Hiroyuki Arai, Shohei Mitani
SCIENTIFIC REPORTS
(2016)
Article
Biology
Samuel Potter, Jacob Sifers, Emily Yocom, Sandra L. E. Bluemich, Rachel Potter, Jeremy Nadolski, Douglas A. Harrison, Robin L. Cooper
Correction
Medicine, Research & Experimental
Woojin Kang, Yuichirou Harada, Kenji Yamatoya, Natsuko Kawano, Seiya Kanai, Yoshitaka Miyamoto, Akihiro Nakamura, Mami Miyado, Yoshiki Hayashi, Yoko Kuroki, Hidekazu Saito, Yasuhiro Iwao, Akihiro Umezawa, Kenji Miyado
LABORATORY INVESTIGATION
(2020)
Article
Medicine, Research & Experimental
Woojin Kang, Yuichirou Harada, Kenji Yamatoya, Natsuko Kawano, Seiya Kanai, Yoshitaka Miyamoto, Akihiro Nakamura, Mami Miyado, Yoshiki Hayashi, Yoko Kuroki, Hidekazu Saito, Yasuhiro Iwao, Akihiro Umezawa, Kenji Miyado
LABORATORY INVESTIGATION
(2020)
Review
Biology
Yoshiki Hayashi, Yuto Yoshinari, Satoru Kobayashi, Ryusuke Niwa
CURRENT OPINION IN INSECT SCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Marika Rikitake, Ayako Matsuda, Daisuke Murata, Katsufumi Dejima, Kazuko H. Nomura, Karen L. Abbott, Shohei Mitani, Kazuya Nomura
JOURNAL OF BIOCHEMISTRY
(2020)
Article
Entomology
Nicole T. Marguerite, Jate Bernard, Douglas A. Harrison, David Harris, Robin L. Cooper
Summary: Thermal receptors like TrpA1 can respond to temperature changes, but their effects on heart function in mammals remain unclear. Fruit fly experiments show that high levels of TrpA1 expression can cause the heart to stop beating at lower temperatures, while blowflies demonstrate increased heart rate at higher temperatures.
Article
Multidisciplinary Sciences
Yuji Suehiro, Sawako Yoshina, Tomoko Motohashi, Satoru Iwata, Katsufumi Dejima, Shohei Mitani
Summary: The study successfully applied the TMP/UV mutagenesis method to C. elegans, creating a gene mutation library and discovering 981 novel mutations in the whole genome. By eliminating false positives through machine learning, the accuracy of mutation identification reached over 95%.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Yoshiki Hayashi, Arisa Shibata, Keisuke Kamimura, Satoru Kobayashi
Summary: The study investigated the functions of syndecan and perlecan in the Drosophila GSC niche and found that while both are important components for GSC maintenance, their effects on GSC numbers are distinct. Interestingly, overexpression of syndecan and perlecan did not cause an expansion of the GSC niche. These findings suggest that HSPG genes contribute to GSC maintenance through multiple mechanisms and pave the way for a deeper understanding of ECM functions in the stem cell niche.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2021)
Article
Nanoscience & Nanotechnology
Robert A. Yokel, Marsha L. Ensor, Hemendra J. Vekaria, Patrick G. Sullivan, David J. Feola, Arnold Stromberg, Michael T. Tseng, Douglas A. Harrison
Summary: This study hypothesized that nanoceria catalyst can modulate inflammation/oxidative stress in macrophages. The results showed that nanoceria blocked the effects of inflammatory factors, altered cell metabolism and oxidative stress, and regulated macrophage phenotype. Nanoceria also affected cell morphology.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Article
Multidisciplinary Sciences
Katsufumi Dejima, Rieko Imae, Yuji Suehiro, Keita Yoshida, Shohei Mitani
Summary: This study investigates the role of dsRNA in gene expression regulation and identifies ZIPT-9 as a negative regulator of systemic RNAi in C. elegans.
Article
Cell Biology
Zachary R. Hettinger, Yuan Wen, Bailey D. Peck, Kyoko Hamagata, Amy L. Confides, Douglas W. Van Pelt, Douglas A. Harrison, Benjamin F. Miller, Timothy A. Butterfield, Esther E. Dupont-Versteegden
Summary: The study found that fibroadipogenic progenitor cells (FAPs) in aged muscle recovering from disuse are enriched in chemotaxis genes but lack ECM remodeling genes, while mechanotherapy can promote ECM remodeling in aged muscle, making gene expression patterns resemble those in adult muscle.
Meeting Abstract
Zoology
N. T. Marguerite, J. Bernard, D. A. Harrison, D. Harris, R. L. Cooper
INTEGRATIVE AND COMPARATIVE BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nanako Kanaki, Ayako Matsuda, Katsufumi Dejima, Daisuke Murata, Kazuko H. Nomura, Takashi Ohkura, Keiko Gengyo-Ando, Sawako Yoshina, Shohei Mitani, Kazuya Nomura