Article
Food Science & Technology
Mengxue Peng, Zitong Zhao, Zhihong Liang
Summary: The strain Brevundimonas naejangsanensis ML17 can simultaneously degrade OTA and OTB, with a degradation rate of 100%. Its extracellular enzyme has a degradation rate of OTA above 90% within the temperature range of 25-55 degrees Celsius, making it a potential detoxifier for ochratoxins.
Article
Food Science & Technology
Yan Yang, Weitong Zhong, Zhonghao Liu, Xianli Xue, Qiang Gao, Depei Wang, Ying Zhang, Jian Zhang
Summary: In this study, a bacterial strain CO29 with high OTA detoxification ability was isolated from vineyards and identified as Cytobacillus oceanisediminis. The removal of OTA by CO29 was mainly attributed to degradation, which was completely removed by boiling and could be significantly reduced by proteinase K treatment. OTA degradation was significantly improved by Zn2+, while inhibited by Ca2+ and Mg2+. Organic solvents inhibited OTA degradation in a dose-dependent manner. Two OTA degradation intermediates were observed, one of which was identified as OT alpha. This is the first report of Cytobacillus oceanisediminis strain with the ability to detoxify OTA.
Article
Agriculture, Multidisciplinary
Melvin S. Samuel, Kanimozhi Jeyaram, Saptashwa Datta, Narendhar Chandrasekar, Ramachandran Balaji, Ethiraj Selvarajan
Summary: Ochratoxins are thermostable fungal metabolites with harmful properties, potentially posing health risks to both animals and humans. Studies have shown various approaches to reduce the content of OTA in agricultural products, such as inhibiting contamination and detoxifying food.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Applied
Mengxue Peng, Zhenzhen Zhang, Xinge Xu, Haoxiang Zhang, Zitong Zhao, Zhihong Liang
Summary: In this study, four novel OTA and OTB degrading enzymes, BnOTase1, BnOTase2, BnOTase3, and BnOTase4, were purified from the metabolites of Brevundimonas naejangsanensis ML17 strain. These enzymes hydrolyzed OTA into OT alpha and OTB into OT beta. They showed no significant cytotoxicity to HEK293 cells, suggesting their potential in mitigating the toxicity of OTA and OTB.
Review
Plant Sciences
Yabin Gao, Hang Fan, Anzheng Nie, Kang Yang, Haiyan Xing, Zhiqing Gao, Liujie Yang, Zheng Wang, Linqi Zhang
Summary: This paper reviews the research progress on the pharmacokinetics, physiological and pathological processes of aconitine, and discusses promising research directions and detoxification methods.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Lan Wang, Xia Hua, Jie Shi, Ninghao Jing, Ting Ji, Bing Lv, Lijun Liu, Yun Chen
Summary: This paper reviews the source, occurrence, and latest achievements in the removal and detoxification of Ochratoxin A (OTA) in food and agricultural products. The influencing factors and mechanisms related to the biodetoxification of OTA are discussed, as well as the advantages, disadvantages, and potential application prospects of these methods.
Review
Food Science & Technology
Lijuan Ding, Meihua Han, Xiangtao Wang, Yifei Guo
Summary: This review summarizes the application of technology in the prevention, removal, and detoxification of OTA, and describes the advantages and disadvantages of each method. The results suggest that biological methods have the greatest potential for degrading OTA.
Article
Pharmacology & Pharmacy
Liye Zhu, Haomiao Wang, Jieyu Yuhan, Boyang Zhang, Hongyu Li, Charles Asakiya, Kunlun Huang, Xiaoyun He, Wentao Xu
Summary: The study found that HEK293 cell-derived exosomes treated with OTA could induce cytotoxicity by increasing ROS levels and decreasing mitochondrial membrane potential, mainly affecting protein metabolism and cell cycle. Furthermore, exosomes extracted by ultracentrifugation were more suitable for experimental use.
Article
Food Science & Technology
Chucen Liu, Chengcheng Zhao, Hujun Liu, Wen Du, Jing Sun, Wenhua Zhou, Changpo Sun
Summary: In this study, two novel strains of Brevibacillus sp., ALJ01 and ALJ02, were isolated from wheat samples and found to have the ability to degrade OTA. The degradation products of OTA were identified as ochratoxin and phenylalanine. These strains could completely degrade OTA within 12 hours and efficiently reduce OTA in wheat products. Strain ALJ02 also showed antagonistic activity against certain OTA-producing fungal species. This study discovered two new strains of Brevibacillus genus with good OTA degradation ability and revealed their potential prospects for biodegradation and biocontrol of OTA.
Article
Green & Sustainable Science & Technology
Supitchaya Jenjaiwit, Nontipa Supanchaiyamat, Andrew J. Hunt, Yuvarat Ngernyen, Thunyalux Ratpukdi, Sumana Siripattanakul-Ratpukdi
Summary: The study demonstrates that using microbial cells immobilized on waste biochar can achieve high TCC removal efficiency in wastewater treatment. The use of cell-immobilized biochar shows significant potential for future utilization and can contribute to the development of a circular bio-based economy using renewable agroindustrial wastes.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Food Science & Technology
Jiangyu Tang, Liuwen Yin, Zhiyong Zhao, Lei Ge, Lili Hou, Yunhuan Liu, Xingxiang Chen, Kehe Huang, Fang Gan
Summary: A new OTA-detoxification strain, Pediococcus acidilactici NJB421, was isolated from cow manure with a removal rate of 48.53% for OTA. This strain showed no adverse effects in mice and alleviated OTA-induced toxicity.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Food Science & Technology
Jessica Audrey Feijo Correa, Tiago de Melo Nazareth, Giuseppe Meca, Fernando Bittencourt Luciano
Summary: This study developed a rapid and affordable bacterial screening assay for detecting ochratoxin A (OTA) degradation capability, saving costs.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Food Science & Technology
Hongwen Xu, Liangzhe Wang, Jiadi Sun, Liping Wang, Hongyan Guo, Yongli Ye, Xiulan Sun
Summary: Mycotoxins are metabolites produced by fungi in food or feed that can be toxic to both humans and animals. Traditional methods for detoxification have limitations, leading to an urgent need for new technologies to effectively control mycotoxins. Microbial detoxification technology has been widely used in recent years due to its high efficiency, low toxicity, and strong specificity.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Chemistry, Applied
Kejia Gu, Dojin Ryu, Hyun Jung Lee
Summary: OTA is a nephrotoxin produced by certain fungal species and commonly found in agricultural commodities. This study examined the thermal stability of OTA in the presence of sugars commonly added to food products, finding that heating OTA with sugars led to changes in its degradation products, with fructose resulting in significantly lower OTA levels and a shift towards less toxic degradation products.
Article
Food Science & Technology
Dong Zou, Jian Ji, Yongli Ye, Yang Yang, Jian Yu, Meng Wang, Yi Zheng, Xiulan Sun
Summary: In this study, a mutated strain of Aspergillus niger with a high degradation rate of ochratoxin A (OTA) was obtained and its degradation conditions were investigated. The degraded products and metabolic pathway of OTA were characterized using liquid chromatography-tandem mass spectrometry combined with mass spectrometry. The results showed that the degradation products had reduced toxicity on HepG2 cells. The application of this mutated strain in real samples demonstrated its effectiveness in removing OTA from wheat bran.
Review
Food Science & Technology
Harsh Kumar, Francine Schutz, Kanchan Bhardwaj, Ruchi Sharma, Eugenie Nepovimova, Daljeet Singh Dhanjal, Rachna Verma, Dinesh Kumar, Kamil Kuca, Natalia Cruz-Martins
Summary: The consumption of probiotics can have both positive and negative effects on health, especially in children. Non-viable probiotics, known as paraprobiotics, have shown to interact with the host and provide health benefits, with enhanced safety in children compared to live probiotics.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Dorota Stary, Eugenie Nepovimova, Kamil Kuca, Marek Bajda
Summary: This study presents a structure-based approach for designing and developing potential mTOR kinase inhibitors. Through structural analysis and in silico studies, key amino acid residues for ligand binding and optimal docking protocols were identified.
CHEMICAL BIOLOGY & DRUG DESIGN
(2023)
Review
Biochemistry & Molecular Biology
Teodorico C. Ramalho, Alexandre A. de Castro, Daniel H. S. Leal, Jessika P. Teixeira, Elaine F. F. da Cunha, Kamil Kuca
Summary: Organophosphorus compounds (OP) are important inhibitors used as pesticides and chemical weapons. They inhibit the acetylcholinesterase (AChE) enzyme, leading to elevated ACh levels and serious adverse effects. Reactivators, particularly oximes, have been developed to combat the toxic effects. However, there is currently no universal antidote for reactivating AChE inhibited by any OP agent. This review discusses the intoxication process, reactivator development, and the use of computational methods and in vitro studies to predict toxicological profiles and support the development process.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Mathematics, Interdisciplinary Applications
Najmeh Pakniyat, Gayathri Vivekanandhan, Karthikeyan Rajagopal, Ondrej Krejcar, Kamil Kuca, Hamidreza Namazi
Summary: One important research area in neuroscience is investigating how brain activity changes during aging. In this study, complexity techniques were used to analyze the age-related changes in brain activity during sleep. By analyzing the EEG signals of 22 subjects induced by sleep medication using fractal theory and sample entropy, it was found that the fractal dimension and sample entropy of EEG signals decrease with aging. Therefore, it was concluded that aging leads to lower complexity in EEG signals during sleep. The employed method of analysis can be applied to study the effects of aging on the activity variations of other organs, such as the heart and muscles, by analyzing their related physiological signals, such as ECG and EMG.
FRACTALS-COMPLEX GEOMETRY PATTERNS AND SCALING IN NATURE AND SOCIETY
(2023)
Article
Environmental Sciences
Sosan Andleeb Khan, Mbah Ntepe Leonel Javeres, Syed Tahir Abbas Shah, Nazia Bibi, Zahid Muneer, Sabir Hussain, Eugenie Nepovimova, Kamil Kuca, Syed Muhammad Nurulain
Summary: This study aimed to investigate the status and role of Butyrylcholinesterase (BChE) in seven different occupational groups. The results showed a significant decrease in BChE activity in gasoline fillers and carpenters, as well as a slight decrease in other occupational groups. Levels of pro-inflammatory cytokines were elevated in all groups. Genotypic analysis revealed a significant association between the K-variant of the BCHE gene and occupational groups.
ENVIRONMENTAL RESEARCH
(2023)
Article
Food Science & Technology
Yu Zhang, Ke-Xin Cao, Qin-Jian Niu, Jiang Deng, Ling Zhao, Mahmoud Mohamed Khalil, Niel Alexander Karrow, Kamil Kuca, Lv-Hui Sun
Summary: This study aimed to identify the key glutathione S-transferase (GST) isozymes involved in the detoxification of Aflatoxin B1 (AFB1) in ducks' primary hepatocytes. The researchers isolated and cloned the full-length cDNA encoding 10 GST isozymes from ducks' liver. The results showed that overexpression of GST and GST3 mitigated the toxic effects of AFB1 and increased the detoxified product of AFB1 in ducks' primary hepatocytes.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tushuai Li, Yue Gu, Baocai Xu, Kamil Kuca, Jie Zhang, Wenda Wu
Summary: CircZBTB44 is upregulated in renal cell carcinoma tissues and its knockdown inhibits RCC cell viability, proliferation, migration, and tumorigenesis. HNRNPC mediates circZBTB44 interaction with IGF2BP3, resulting in upregulation of HK3 and promotion of RCC cell malignant behaviors. This study provides new insights into targeted therapy for RCC.
Review
Plant Sciences
Reema Devi, Rachna Verma, Rajni Dhalaria, Ashwani Kumar, Dinesh Kumar, Sunil Puri, Monika Thakur, Saurav Chauhan, Prem Parkash Chauhan, Eugenie Nepovimova, Kamil Kuca
Summary: Endophytic fungi, as plant mutualists, have beneficial impacts on the environment and commercial applications. They enhance the biological properties of plants by producing various bioactive molecules and enzymes. These fungi can exist asymptomatically within plant tissues and play a crucial role in enhancing plant properties as well as human health.
Article
Pharmacology & Pharmacy
Vesna Jacevic, Jelena Dumanovic, Suliman Y. Alomar, Radmila Resanovic, Zoran Milovanovic, Eugenie Nepovimova, Qinghua Wu, Tanos Celmar Costa Franca, Wenda Wu, Kamil Kuca
Summary: Contamination of food products with aflatoxins (AFs) poses serious health risks worldwide, primarily dependent on the concentration of consumed AFs. Risk assessment guidelines established by regulatory bodies in different countries help prevent aflatoxin intoxication and protect public health. Rational risk management decisions require consideration of factors such as toxicological profile, exposure duration, analytical techniques, socioeconomic factors, food intake patterns, and maximum allowable levels of each aflatoxin in different food products, which may vary between countries.
Article
Computer Science, Artificial Intelligence
Xiang Li, Zeshui Xu, Hai Wang, Ondrej Krejcar, Kamil Kuca, Enrique Herrera-Viedma
Summary: In this paper, a novel multi-criteria three-way decision (TWD) method is proposed to solve classification and sorting problems by incorporating both qualitative and quantitative information. Traditional TWD theories often neglect the requirements and targets of decision-makers, thus a parameter is designed to reflect the preference degree of the decision-maker's requirements. Using a combination of real numbers and interval numbers for quantitative information and interval-valued double hierarchy linguistic term sets for qualitative information, the proposed method demonstrates its effectiveness in a case study on the selection of algorithm engineers in recruitment process, outperforming other methods.
EXPERT SYSTEMS WITH APPLICATIONS
(2023)
Article
Food Science & Technology
Luying Zhang, Xu Wang, Eugenie Nepovimova, Qinghua Wu, Wenda Wu, Kamil Kuca
Summary: Trichothecene mycotoxin deoxynivalenol (DON) negatively regulates immune response by damaging host immune system and harming the organism's health. We hypothesized that DON can initiate an active immunosuppressive mechanism similar to immune evasion to alter the cellular microenvironment and evade immune surveillance.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Food Science & Technology
Yi-Xin Mo, Meng-Ling Ruan, Jie Wang, Ying Liu, Yuan-Yuan Wu, Guan-Lin Wang, Yan-Ming Han, Hai-Feng Wan, Dessalegn Lamesgen, Kamil Kuca, Jiang Deng, Lv-Hui Sun
Summary: This study evaluated the efficacy of TOXO-XL, an integrated mycotoxin-mitigating agent, in mitigating aflatoxin B1 (AFB1)-induced damage in three cell lines: Leghorn male hepatoma (LMH), porcine jejunum epithelial cell line (IPEC-J2), and porcine alveolar macrophages (3D4/21). The results showed that XL supplementation could ameliorate the cytotoxic effects of AFB1, including cell viability, ROS, apoptosis, DNA damage, migration, and phagocytosis abilities. XL also attenuated AFB1-induced inflammatory response and reversed the alterations in apoptotic signaling. Additionally, XL may have a protective effect on the intestinal barrier by restoring occludin protein.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Food Science & Technology
Ben Wei, Huiping Xiao, Baocai Xu, Kamil Kuca, Zihui Qin, Xinyi Guo, Wenda Wu, Qinghua Wu
Summary: Type B trichothecenes, including DON and four congeners, cause emetic reactions in human and animal due to food contamination. PYY and 5-HT play key roles in the emetic reactions induced by these mycotoxins.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Haoyu Gao, Eugenie Nepovimova, Zbynek Heger, Marian Valko, Qinghua Wu, Kamil Kuca, Vojtech Adam
Summary: Senescent cells secrete harmful molecules that damage surrounding cells, leading to age-related diseases. Hypoxia has been found to play a critical role in cellular senescence by regulating the levels of senescence markers. In addition, gut microbiota and exosomal RNAs are involved in cellular senescence. This review provides new insights into hypoxia-mediated cellular senescence and potential strategies for anti-aging and disease treatment.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Environmental Sciences
Sara Bibi, Rabia Habib, Sania Shafiq, Syed Sayyam Abbas, Shaiza Khan, Syed Ali Musstjab Akber Shah Eqani, Eugenie Nepovimova, Mansoor Shaukat Khan, Kamil Kuca, Syed Muhammad Nurulain
Summary: This study aimed to investigate the impact of chronic consumption of fluoride contained groundwater on human subjects. Through examination and analysis of 512 volunteers from different areas of Pakistan, it was found that participants from fluoride endemic areas exhibited symptoms of dental and skeletal fluorosis. Cholinergic enzymes, ACHE gene 3'-UTR variant, and BCHE K-variant were identified to be associated with the risk of fluorosis. Increased levels of pro-inflammatory cytokines were also observed, which had a significant correlation with fluoride exposure and cholinergic enzymes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)