Article
Immunology
Yongchong Peng, Xiaojie Zhu, Lin Gao, Jieru Wang, Han Liu, Tingting Zhu, Yifan Zhu, Xin Tang, Changmin Hu, Xi Chen, Huanchun Chen, Yingyu Chen, Aizhen Guo
Summary: This study aimed to identify the role of a novel protein Rv0309 in Mycobacterium tuberculosis in inhibiting the host inflammatory response and enhancing bacterial survival. Rv0309 was found to decrease cell wall permeability and suppress cytokine production through NF-κB and MAPK signaling pathways.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Yingyu Chen, Wenjun Zhai, Kailun Zhang, Han Liu, Tingting Zhu, Li Su, Luiz Bermudez, Huanchun Chen, Aizhen Guo
Summary: This study examined the differential expression of sRNAs in Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guerin (BCG) infection models, finding that many were associated with lipid metabolism. One particular sRNA, ncBCG427, was significantly downregulated when BCG entered macrophages and was linked to increased biofilm formation. The results suggest that virulence reduction in mycobacteria may be dependent on the regulation of lipid metabolism.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Miquel de Homdedeu, Lidia Sanchez-Moral, Concepcio Violan, Neus Rafols, Dan Ouchi, Berta Martin, Miguel A. Peinado, Alheli Rodriguez-Cortes, Marta Arch-Sisquella, Daniel Perez-Zsolt, Jordana Munoz-Basagoiti, Nuria Izquierdo-Useros, Betlem Salvador, Joan Matllo, Sergi Lopez-Serrano, Joaquim Segales, Cristina Vilaplana, Pere Toran-Monserrat, Rosa Morros, Ramon Monfa, Maria-Rosa Sarrias, Pere-Joan Cardona
Summary: The study aims to analyze whether heat-killed environmental mycobacteria can induce trained immunity against SARS-CoV-2 infection. The results show that heat-killed mycobacteria induce cellular immune changes in vitro, but no similar effects were observed when orally administered in vivo.
Article
Microbiology
Tong Qiu, Guangping Luo, Jinfeng Jiang, Ping Ding, Qintong Li
Summary: This study establishes an industrial strain of Mycobacterium phlei (M. phlei) and characterizes its genomic, metabolic, and immunological profiles. The strain has been stably passed for more than a decade and shows potential as an immunomodulating agent. Immunophenotyping and metabolomic analysis demonstrate immune responses and consistent metabolic profiles in mice treated with the strain. The results suggest highly reproducible production of M. phlei under GMP conditions.
MICROBIOLOGY SPECTRUM
(2022)
Article
Immunology
Giovanni Ghielmetti, Anne M. Kupca, Matthias Hanczaruk, Ute Friedel, Hubert Weinberger, Sandra Revilla-Fernandez, Erwin Hofer, Julia M. Riehm, Roger Stephan, Walter Glawischnig
Summary: This article reports three cases of M. microti infections in free-ranging red deer, with manifestations including pyogranulomatous pleuropneumonia and granulomas on the pericardium. Phylogenetic analysis revealed independent infection events without host-adapted genotype. Awareness of the pathogenicity and zoonotic potential of M. microti is important for personnel involved in bovine tuberculosis-monitoring programs.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Immunology
Yingying Cui, Guanghui Dang, Hui Wang, Yiyi Tang, Mingyue Lv, Siguo Liu, Ningning Song
Summary: In this study, the role and mechanism of DosR in Mycobacterium bovis Bacillus Calmette-Guerin (BCG) were investigated using omics analysis. The results showed that deleting DosR significantly affected the transcriptional levels of genes and proteins. Targeted metabolomics data revealed that DosR knockout upregulated L-Aspartic acid and serine synthesis while downregulating seven other amino acids. These findings suggest that DosR regulates amino acid synthesis and metabolism, providing insights into its novel regulatory target.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Masanori Asai, Gerard Sheehan, Yanwen Li, Brian D. Robertson, Kevin Kavanagh, Paul R. Langford, Sandra M. Newton
Summary: The study used the larvae of G. mellonella as a non-mammalian infection model for the Mycobacterium tuberculosis complex, characterizing the immune response through proteomic and transcriptomic approaches. The results showed distinct changes in the proteome at different time points post-infection, with inconsistencies between gene expression and protein abundance. Insights into the innate immune response and potential antimycobacterial agents were gained, validating the insect model as a cost-effective and ethically acceptable tool for studying MTBC members.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Elena Mata, Raquel Tarancon, Claudia Guerrero, Eduardo Moreo, Flavie Moreau, Santiago Uranga, Ana Belen Gomez, Dessislava Marinova, Miriam Domenech, Fernando Gonzalez-Camacho, Marta Monzon, Juan Badiola, Jorge Dominguez-Andres, Jose Yuste, Alberto Anel, Antonio Peixoto, Carlos Martin, Nacho Aguilo
Summary: In this study, pulmonary delivery of BCG was shown to preactivate alveolar macrophages, leading to efficient containment of Mtb and potential protection against respiratory pathogens. The results suggest the generation of a trained innate memory-like response in alveolar macrophages induced by pulmonary BCG vaccination, even after vaccine clearance. Additionally, BCG-induced innate activation also provided heterologous protection against Streptococcus pneumoniae challenge.
SCIENCE IMMUNOLOGY
(2021)
Article
Microbiology
Shannon C. Duffy, Manigandan Venkatesan, Shubhada Chothe, Indira Poojary, Valsan Philip Verghese, Vivek Kapur, Marcel A. Behr, Joy Sarojini Michael
Summary: The study describes the development of a two-step multiplex real-time PCR assay to detect and identify BCG strains. The assay has shown potential in accurately identifying BCG from cultures and infected tissue, offering a rapid method for diagnosis and patient management. Identifying and differentiating BCG strains may also be valuable for BCG vaccine pharmacovigilance.
MICROBIOLOGY SPECTRUM
(2021)
Review
Immunology
Neelam Mukherjee, Esther Julian, Jordi B. Torrelles, Robert S. Svatek
Summary: The BCG vaccine, originally developed to protect against active tuberculosis, has shown impressive antitumor activity, particularly in preventing bladder cancer. Recent studies indicate the importance of both innate and adaptive cell responses in BCG therapy, with cells like NK cells, macrophages, dendritic cells, neutrophils, as well as T cells playing key roles in eradicating cancer cells.
Article
Microbiology
Mengjin Qu, Zhengmin Liang, Yulan Chen, Yuanzhi Wang, Haoran Wang, Ziyi Liu, Yiduo Liu, Yuhui Dong, Xin Ge, Hao Li, Xiangmei Zhou
Summary: Research shows that antibodies targeting the cell wall of M. bovis can protect against infection by promoting phagocytosis, inhibiting bacterial growth, and enhancing phagosome-lysosome fusion. This study highlights the importance of these antibodies in defense against tuberculosis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ming Li, Yoshiyuki Yamada, G. Marcela Rodriguez, Thomas Dick, Mei Lin Go
Summary: The research demonstrates that the redox cycler SA23, by generating reactive oxygen species (ROS), simulates an iron-deficient state in bacteria and enhances its bactericidal activity by increasing the expression of iron acquisition genes. Further exacerbating iron deficiency or oxidative stress enhances the activity of SA23. This study suggests that redox cyclers that perturb iron homeostasis and cellular respiration could be potent next-generation anti-tubercular drugs.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biotechnology & Applied Microbiology
Andreon Santos Machado da Silva, Lawrence Henrique Paz Albuquerque, Carlos Germano Garrido de Ponte, Matheus Rogerio de Almeida, Sandra Elizabete Ribeiro de Faria, Mariana da Silva Ribeiro, Evelyn Nunes Goulart da Silva Pereira, Paulo Renato Zuquim Antas
Summary: Tuberculosis is a major public health issue globally, with the Bacillus Calmette-Guerin (BCG) vaccine being the only available vaccine against it. Different strains of BCG vaccine have varying effects on host immune responses, with the BCG Moreau vaccine showing a higher inflammatory cytokine profile and lower TGF-beta levels compared to other strains like Russia, Pasteur, and Danish strains. The differences in vaccine strains may influence the activation of host cells and the protective efficacy in combating TB.
HUMAN VACCINES & IMMUNOTHERAPEUTICS
(2022)
Article
Immunology
Reginaldo G. Bastos, Heba F. Alzan, Vignesh A. Rathinasamy, Brian M. Cooke, Odir A. Dellagostin, Raul G. Barletta, Carlos E. Suarez
Summary: This article discusses the potential use of BCG TI as a strategy to control babesiosis infection more efficiently, supported by experimental evidence and the possibility of vaccine development.
Article
Cell Biology
Yinjuan Song, Xin Ge, Yulan Chen, Tariq Hussain, Zhengmin Liang, Yuhui Dong, Yuanzhi Wang, Chengyuan Tang, Xiangmei Zhou
Summary: Mitophagy, a selective autophagy mechanism for eliminating damaged mitochondria, plays a crucial role in immune evasion. Mycobacterium bovis utilizes host mitophagy to suppress xenophagy, enhancing its intracellular survival. The PINK1-PRKN/Parkin pathway is involved in M. bovis-induced mitophagy, with inhibition of mitophagy restricting bacterial survival in macrophages and mouse lungs. Induction of mitophagy competes with host xenophagy, highlighting a new insight into the intracellular survival mechanism of M. bovis.
Article
Immunology
Reto Guler, Thabo Mpotje, Mumin Ozturk, Justin K. Nono, Suraj P. Parihar, Julius Ebua Chia, Nada Abdel Aziz, Lerato Hlaka, Santosh Kumar, Sugata Roy, Adam Penn-Nicholson, Willem A. Hanekom, Daniel E. Zak, Thomas J. Scriba, Harukazu Suzuki, Frank Brombacher
MUCOSAL IMMUNOLOGY
(2019)
Article
Medicine, General & Internal
Adam Penn-Nicholson, Thomas Hraha, Ethan G. Thompson, David Sterling, Stanley Kimbung Mbandi, Kirsten M. Wall, Michelle Fisher, Sara Suliman, Smitha Shankar, Willem A. Hanekom, Nebojsa Janjic, Mark Hatherill, Stefan H. E. Kaufmann, Jayne Sutherland, Gerhard Walzl, Mary Ann De Groote, Urs Ochsner, Daniel E. Zak, Thomas J. Scriba
Editorial Material
Infectious Diseases
Ole F. Olesen, Fareed Abdullah, Rene Coppens, Peter J. Gardner, Ann M. Ginsberg, Willem A. Hanekom, Hannu Laang, David M. Lewinsohn, Glaudina Loots, Alexander Schmidt, Johan Vekemans, Gerald H. Voss
LANCET INFECTIOUS DISEASES
(2019)
Correction
Medicine, General & Internal
Adam Penn-Nicholson, Thomas Hraha, Ethan G. Thompson, David Sterling, Stanley Kimbung Mbandi, Kirsten M. Wall, Michelle Fisher, Sara Suliman, Smitha Shankar, Willem A. Hanekom, Nebojsa Janjic, Mark Hatherill, Stefan H. E. Kaufmann, Jayne Sutherland, Gerhard Walzl, Mary Ann De Groote, Urs Ochsner, Daniel E. Zak, Thomas J. Scriba
Article
Immunology
Marc Tebruegge, Nicole Ritz, Susan Donath, Binita Dutta, Benjamin Forbes, Vanessa Clifford, Christel Zufferey, Robert De Rose, Roy M. Robins-Browne, Willem Hanekom, Stephen M. Graham, Tom Connell, Nigel Curtis
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Immunology
Sara Suliman, Melissa Murphy, Munyaradzi Musvosvi, Anele Gela, Erin W. Meermeier, Hennie Geldenhuys, Christiaan Hopley, Asma Toefy, Nicole Bilek, Ashley Veldsman, Willem A. Hanekom, John L. Johnson, W. Henry Boom, Gerlinde Obermoser, Huang Huang, Mark Hatherill, David M. Lewinsohn, Elisa Nemes, Thomas J. Scriba
JOURNAL OF IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Adam Penn-Nicholson, Stanley Kimbung Mbandi, Ethan Thompson, Simon C. Mendelsohn, Sara Suliman, Novel N. Chegou, Stephanus T. Malherbe, Fatoumatta Darboe, Mzwandile Erasmus, Willem A. Hanekom, Nicole Bilek, Michelle Fisher, Stefan H. E. Kaufmann, Jill Winter, Melissa Murphy, Robin Wood, Carl Morrow, Ildiko Van Rhijn, Branch Moody, Megan Murray, Bruno B. Andrade, Timothy R. Sterling, Jayne Sutherland, Kogieleum Naidoo, Nesri Padayatchi, Gerhard Walzl, Mark Hatherill, Daniel Zak, Thomas J. Scriba
SCIENTIFIC REPORTS
(2020)
Article
Microbiology
Esther van Woudenbergh, Edward B. Irvine, Leela Davies, Marwou de Kock, Willem A. Hanekom, Cheryl L. Day, Sarah Fortune, Galit Alter
Article
Multidisciplinary Sciences
Maria Papadopoulou, Tanya Dimova, Muki Shey, Libby Briel, Helen Veldtsman, Nondumiso Khomba, Hadn Africa, Marcia Steyn, Willem A. Hanekom, Thomas J. Scriba, Elisa Nemes, David Vermijlen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Immunology
Elisa Nemes, Shabaana A. Khader, Rosemary Swanson, Willem A. Hanekom
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Medicine, General & Internal
Mark J. Siedner, John D. Kraemer, Mark J. Meyer, Guy Harling, Thobeka Mngomezulu, Patrick Gabela, Siphephelo Dlamini, Dickman Gareta, Nomathamsanqa Majozi, Nothando Ngwenya, Janet Seeley, Emily Wong, Collins Iwuji, Maryam Shahmanesh, Willem Hanekom, Kobus Herbst
Article
Immunology
Gwendolyn M. Swarbrick, Anele Gela, Meghan E. Cansler, Megan D. Null, Rowan B. Duncan, Elisa Nemes, Muki Shey, Mary Nsereko, Harriet Mayanja-Kizza, Sarah Kiguli, Jeffrey Koh, Willem A. Hanekom, Mark Hatherill, Christina Lancioni, David M. Lewinsohn, Thomas J. Scriba, Deborah A. Lewinsohn
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Biology
Virginie Rozot, Elisa Nemes, Hennie Geldenhuys, Munyaradzi Musvosvi, Asma Toefy, Frances Rantangee, Lebohang Makhethe, Mzwandile Erasmus, Nicole Bilek, Simbarashe Mabwe, Greg Finak, William Fulp, Ann M. Ginsberg, David A. Hokey, Muki Shey, Sanjay Gurunathan, Carlos DiazGranados, Linda-Gail Bekker, Mark Hatherill, Thomas J. Scriba
COMMUNICATIONS BIOLOGY
(2020)
Article
Critical Care Medicine
Laure F. Pittet, Nora Fritschi, Marc Tebruegge, Binita Dutta, Susan Donath, Nicole L. Messina, Dan Casalaz, Willem A. Hanekom, Warwick J. Britton, Roy Robins-Browne, Nigel Curtis, Nicole Ritz
Summary: The presence of a scar after BCG vaccination is associated with stronger mycobacteria-specific T-cell responses. Further study on the relationship between scar formation and protection against tuberculosis as well as the need for revaccination in scar-negative individuals is important.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Medicine, Research & Experimental
Julius Muller, Rachel Tanner, Magali Matsumiya, Margaret A. Snowden, Bernard Landry, Iman Satti, Stephanie A. Harris, Matthew K. O'Shea, Lisa Stockdale, Leanne Marsay, Agnieszka Chomka, Rachel Harrington-Kandt, Zita-Rose Manjaly Thomas, Vivek Naranbhai, Elena Stylianou, Stanley Kimbung Mbandi, Mark Hatherill, Gregory Hussey, Hassan Mahomed, Michele Tameris, J. Bruce McClain, Thomas G. Evans, Willem A. Hanekom, Thomas J. Scriba, Helen McShane, Helen A. Fletcher