Article
Multidisciplinary Sciences
Nichola J. Starr, Mohammed H. Khan, Max K. Edney, Gustavo F. Trindade, Stefanie Kern, Alexander Pirkl, Matthias Kleine-Boymann, Christopher Elms, Mark M. O'Mahony, Mike Bell, Morgan R. Alexander, David J. Scurr
Summary: In this study, the 3D OrbiSIMS technique was used to analyze the molecular chemistry of human skin tissue and provide unprecedented details about the nonhomogeneity of the stratum corneum. The analysis resolved debates about chemical gradients within the epidermis and revealed similarities in epidermal compounds between human and porcine skin. Additionally, the study demonstrated the permeation of an antiaging peptide into the human stratum corneum.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Adrienn Jenei, Gergo Kallo, Zsolt Dajnoki, Krisztian Gaspar, Andrea Szegedi, Aniko Kapitany, Eva Csosz
Summary: This study developed a novel approach for examining AMPs in the outer layer of the epidermis, with hexane-free processing producing higher protein yield. Among the investigated peptides, 9 could be detected in healthy or inflamed skin, and significant topographical differences were observed in the amount of certain peptides among different regions of healthy skin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Joudi Bakar, Rime Michael-Jubeli, Rindala El Khoury, Sabrina Hamla, Ali Assi, Arlette Baillet-Guffroy, Ali Tfayli
Summary: This study aimed to characterize the molecular and functional features of reconstructed human epidermis models using various techniques, focusing on the barrier function, structure, and lipid composition changes during keratinocyte differentiation. The results highlighted the decrease in caffeine penetration, increase in stratum corneum thickness, lipid organization evolution, and ceramide changes throughout the differentiation process. Different behaviors of ceramide subclasses were observed, with a shift between lipid generation and organization identified. Three maturation groups were described based on analytical data, providing insights into the barrier function at different stages of differentiation.
Review
Biochemistry & Molecular Biology
Joke A. Bouwstra, Andreea Nadaban, Wim Bras, Clare McCabe, Annette Bunge, Gerrit S. Gooris
Summary: The barrier function of the skin is primarily located in the outermost layer called stratum corneum (SC), which contains lipid lamellae that play a crucial role in permeation of compounds. Analyzing the lipid composition is essential in understanding the skin barrier function and its impairment in inflammatory skin diseases.
PROGRESS IN LIPID RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Andrej Kovacik, Petra Pullmannova, Lukas Opalka, Michaela Silarova, Jaroslav Maixner, Katerina Vavrova
Summary: The study shows that different types of ceramides play distinct roles in the structural organization and function of the skin lipid barrier, with their effects on lipid models varying depending on the sphingoid base chain and configuration at the alpha-carbon.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Harley Robinson, Jeffrey Molendijk, Alok K. Shah, Tony Rahman, Gregory J. Anderson, Michelle M. Hill
Summary: This study comprehensively evaluated the accuracy of lipid quantification using Attenuated Total Reflectance (ATR)-Fourier-Transform Infrared Spectroscopy (FTIR) and established a simple workflow for lipidomics sample quantification. The impact of sample extraction method and FTIR spectral processing on lipid quantification was investigated. A Lipid Quality (LiQ) score was developed for sample quality screening. The workflow was validated by comparing with LC-MS quantification results.
Article
Multidisciplinary Sciences
Chao-Chun Yang, Yun-Yo Yen, Chao-Kai Hsu, Nan-Yu Cheng, Shih-Yu Tzeng, Shih-Jay Chou, Jun-Ming Chang, Sheng-Hao Tseng
Summary: This study investigated near infrared spectroscopic features of psoriatic and normal skin, revealing significant differences that could be linked to the epidermal barrier function. It was found that using diffuse reflectance spectroscopy could achieve objective evaluation of the epidermal barrier function of psoriasis.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Physical
Yao Chen, Mingrui Liao, Kun Ma, Zi Wang, Bruno Deme, Jeff Penfold, Jian R. Lu, John R. P. Webster, Peixun Li
Summary: This study investigated how surfactants interact with a lipid SC model using neutron diffraction and MD simulations, revealing the impact of surfactant molecular architecture on their interaction. It demonstrated that surfactants can enhance membrane hydration and reduce phase separated crystalline cholesterol, depending on the surfactant chain length, branching, and double bond, providing useful guidance for selecting or designing surfactants for transdermal applications.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Medicine, Research & Experimental
Alexander A. Merleev, Stephanie T. Le, Claire Alexanian, Atrin Toussi, Yixuan Xie, Alina Marusina, Steven M. Watkins, Forum Patel, Allison C. Billi, Julie Wiedemann, Yoshihiro Izumiya, Ashish Kumar, Ranjitha Uppala, J. Michelle Kahlenberg, Fu-Tong Liu, Iannis E. Adamopoulos, Elizabeth A. Wang, Chelsea Ma, Michelle Y. Cheng, Halani Xiong, Amanda Kirane, Guillaume Luxardi, Bogi Andersen, Lam C. Tsoi, Carlito B. Lebrilla, Johann E. Gudjonsson, Emanual Maverakis
Summary: This study characterized the biogeographic alterations of human epidermal lipids across different body sites and compared the gene expression of keratinocytes. The findings highlight the unique adaptation of the epidermis to environmental insults and the impact of inflammation-associated gene expression changes on the epidermal lipidome.
Article
Biochemistry & Molecular Biology
Madoka Suzuki, Yusuke Ohno, Akio Kihara
Summary: This study used LC/MS/MS analysis to identify 1,327 unbound ceramides and 254 protein-bound ceramides in the stratum corneum. The researchers discovered a wide variety of ceramide species with different long-chain bases in the human skin, and demonstrated the production of specific long-chain bases by one of the enzymes. This study provides a molecular basis for understanding ceramide diversity in the skin and the pathogenesis of skin disorders.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Charlotte M. Beddoes, Denise E. Rensen, Gert S. Gooris, Marc Malfois, Joke A. Bouwstra
Summary: The study found that the effects of FFA chain lengths on lamellar phases and lateral packing vary, with a threshold for water permeability at FFA C16.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Madoka Suzuki, Yusuke Ohno, Akio Kihara
Summary: This study conducted LC/MS/MS analyses to reveal various ceramide species in the human stratum corneum, quantifying a large number of ceramides and providing a molecular basis for understanding the diversity of human skin ceramides and the pathogenesis of skin disorders.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Cell Biology
Takayuki Sassa, Akio Kihara
Summary: The permeability barrier in the oral cavity is crucial for protection against infection. This study reveals the presence of u-O-acylceramides and protein-bound ceramides, which are essential for barrier formation, in the oral mucosae, esophagus, and stomach in mice. Conditional knockout of the fatty acid elongase Elovl1 in the oral mucosae and esophagus leads to increased pigment penetration and enhanced aversive responses. Acylceramides and protein-bound ceramides are also found in human oral mucosae. These findings highlight the importance of acylceramides and protein-bound ceramides in oral barrier formation.
Review
Dermatology
Lars Norlen, Magnus Lundborg, Christian Wennberg, Ali Narangifard, Bertil Daneholt
Summary: The skin's barrier, composed of a lipid structure in the stratum corneum, plays a crucial role in protecting the body from the environment. Recent advancements in cryo-EM have allowed for a better understanding of the architecture and formation process of this barrier.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Pharmacology & Pharmacy
Aneta Vovesna, Alexander Zhigunov, Martin Balouch, Jarmila Zbytovska
Summary: The study indicated that stable liposomal systems containing ceramides and other skin barrier lipids are effective in repairing a disrupted skin barrier, and non-homogenized liposomes are more effective than their homogenized counterparts.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Biochemistry & Molecular Biology
Raja Narayanasamy, Dandamudi Usharani, Ram Rajasekharan
Summary: This study investigated the role of ABHD16B in lipid metabolism. The overexpression of ABHD16B was found to decrease cellular triacylglycerol levels and increase phospholipid synthesis in yeast cells. Additionally, ABHD16B overexpression led to a reduction in lipid droplets and significant modifications in fatty acid composition. These findings highlight the importance of ABHD16B in lipid homeostasis and provide insights into its regulatory function in cellular lipid metabolism.
CHEMISTRY AND PHYSICS OF LIPIDS
(2024)
