Article
Agriculture, Multidisciplinary
Yulin Zhao, Haoyan Huang, Ning Lv, Chunyan Huang, Huili Chen, Han Xing, Chaorui Guo, Ning Li, Di Zhao, Xijing Chen, Yongjie Zhang
Summary: In this study, the thiol-GSH conjugates of GP, termed M532-1 and M532-2, were identified for the first time. The catalytic activities of GSTs were investigated both in vitro and in vivo. It was found that GSTA1-1 and GSTA4-4 exhibited high activity in the formation of both thiol-GSH conjugates, with GSTA4-4 specifically catalyzing M532-2 formation in vitro. The study demonstrated the inactivation of GP by GSTs and suggested that interindividual variability of GSTs may be a risk factor for susceptibility to GE-induced hepatotoxicity.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Mathieu Schwartz, Helene Brignot, Gilles Feron, Thomas Hummel, Yunmeng Zhu, Dorothee von Koskull, Jean-Marie Heydel, Federic Lirussi, Francis Canon, Fabrice Neiers
Summary: The molecules that cause taste sensation interact with taste receptors in the taste buds. Enzymes involved in detoxification processes exist in saliva as well as in type II cells where taste receptors, including bitter taste receptors, are located. These enzymes are known to have interactions with various molecules. The study shows that salivary glutathione transferases (GSTA1 and GSTP1) can metabolize bitter molecules, and their levels in the saliva of people with taste disorders are significantly lower than those in the control group.
Article
Genetics & Heredity
Xiaoyu Duan, Xuejing Yu, Yidan Wang, Wei Fu, Ruifang Cao, Lu Yang, Xueling Ye
Summary: This study identified and analyzed 46 GST genes in cucumber, and found that most of these genes respond to cold stress. GO and KEGG analysis suggested that these genes may respond to cold stress through glutathione metabolism.
FRONTIERS IN GENETICS
(2022)
Review
Biochemistry & Molecular Biology
Ning Lv, Chunyan Huang, Haoyan Huang, Zhiqiang Dong, Xijing Chen, Chengcan Lu, Yongjie Zhang
Summary: Glutathione S-transferases (GSTs) are important metabolic enzymes that play essential roles in detoxification and have diverse activities in various diseases. Much research has been done on GST overexpression in tumor drug resistance, leading to the development of GST inhibitors for the treatment of drug-resistant tumors. In recent years, novel roles of GSTs in other diseases have also been recognized. This review summarizes the roles of GSTs in diseases, inventories specific GST inhibitors, and discusses their potential applications in targeting cell signaling pathways and intracellular processes.
Article
Biochemistry & Molecular Biology
Zhi-Xiang Liu, Xiao-Rong Xing, Xin-Hao Liang, Jian-hao Ding, Yi-Jiangcheng Li, Ying Shao, Fu-An Wu, Jun Wang, Sheng Sheng
Summary: Glyphodes pyloalis, a destructive pest on mulberry trees, poses a significant threat to the sericultural industry in China. This study reveals the important roles of glutathione-S-transferases (GSTs) in the tolerance of the insecticides phoxim and chlorfenapyr in G. pyloalis.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemical Research Methods
Yuxing Zhang, Deliang Huang, Ning Lv, Guilan Zhu, Jinghan Peng, Tiansheng Chou, Zhibin Zhu, Ju Wang, Yuanyuan Chen, Xiangdong Fang, Jiuxin Qu, Jun Chen, Siqi Liu
Summary: The concentration of glutathione S-transferase (GST) in serum is considered as a potential biomarker for disease diagnosis. This study developed a new method for the enrichment and quantification of serum GSTs, which has apparent clinical potential for disease diagnosis.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Plant Sciences
Irfan Ali Sabir, Muhammad Aamir Manzoor, Iftikhar Hussain Shah, Xunju Liu, Songtao Jiu, Jiyuan Wang, Pravej Alam, Muhammad Abdullah, Caixi Zhang
Summary: In this study, 67 GST genes were identified in the sweet cherry genome, classified into seven chief subfamilies according to phylogenetic tree analysis. Most PavGST genes showed relatively well-maintained exon-intron and motif arrangement within the same group, as revealed by gene structure and motif analyses. The findings provide a foundation for future research on LED-induced anthocyanin and antioxidants accumulation in sweet cherries.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yongheng Zhang, Jingyuan He, Yezi Xiao, Yingao Zhang, Yingqin Liu, Siqing Wan, Lu Liu, Yuan Dong, Huan Liu, Youben Yu
Summary: The study demonstrates that in tea plants, the CsGSTU8 gene is upregulated in response to drought stress and ABA treatment, playing a critical role in enhancing drought tolerance. It also reveals that CsWRKY48 functions as a transcriptional activator and activates the expression of CsGSTU8, providing new insights into drought stress responses in tea plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yuwei Cao, Leifeng Xu, Hua Xu, Panpan Yang, Guoren He, Yuchao Tang, Xianyu Qi, Meng Song, Jun Ming
Summary: The study identified the LhGST gene in Asiatic hybrid lilies, which may be involved in anthocyanin sequestration and transport in lily tepals. Functional analysis confirmed the role of LhGST in anthocyanin accumulation. Additionally, the promoter of LhGST contains various hormone- and stress-responsive elements, and is regulated by a MYB transcription factor.
PLANT CELL REPORTS
(2021)
Article
Plant Sciences
Yize Zou, Shihan Cao, Bochui Zhao, Zhonghua Sun, Liru Liu, Mingshan Ji
Summary: Weeds, specifically Sagittaria trifolia in Liaoning, China, have developed resistance to the herbicide bensulfuron-methyl, causing significant yield losses in rice. This study explores the mechanisms underlying this resistance, focusing on the non-target-site resistance mechanisms involving glutathione S-transferase (GST) and reactive oxygen species (ROS) scavenging.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Entomology
Marija Vukcevic, Aleksandra Nikolic-Kokic, Ivan Aleksic, Sanja Todorovic, Zorana Orescanin-Dusic, Dusko Blagojevic, Dragana Despot
Summary: Controlling the number of ticks as carriers of infectious diseases is important, but their protective mechanisms can complicate the process. We investigated the relationship between tick abundance and glutathione-S-transferases activity (GSTs) after pyrethroid treatment. We collected ticks from six locations in Belgrade, tested their GSTs activity, and correlated it with tick numbers. The results showed a correlation between tick abundance and GSTs activity. Treatment efficiency varied by location but was similar in both spring and autumn. GSTs activity reflects the influence of local chemicals and pyrethroid, indicating its potential for improving tick removal practices.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Plant Sciences
Madhu, Alok Sharma, Amandeep Kaur, Shivi Tyagi, Santosh Kumar Upadhyay
Summary: This review discusses the importance of glutathione peroxidases (GPXs) in plant physiology, including their role in scavenging reactive oxygen species, maintaining redox homeostasis, and responding to environmental stresses. The study of GPX genes provides valuable information for genetic engineering to enhance agricultural plant yields under unfavorable conditions.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Agronomy
Guojun Zhang, Linqin Meng, Rui Chen, Wen Wang, Xiangfeng Jing, Keyan Zhu-Salzman, Weining Cheng
Summary: This study characterized delta and epsilon GST genes in S. mosellana for the first time, showing increased expression in larvae feeding on resistant wheat varieties. The tissue-specific expression and metabolic capabilities of these SmGSTs suggest their importance in detoxifying wheat defensive compounds during feeding, potentially contributing to host plant adaptation in this pest species.
PEST MANAGEMENT SCIENCE
(2023)
Article
Medicine, Research & Experimental
Cheng-Huang Shen, Jin-Yi Wu, Shou-Chieh Wang, Chi-Hung Wang, Chen-Tai Hong, Pei-Yu Liu, Sin-Rong Wu, Yi-Wen Liu
Summary: Glutathione S-transferases (GSTs) play an important role in metabolism and detoxification of various substances. A study found that GSTM2 gene expression is lower in advanced stages of bladder urothelial carcinoma, and overexpression of GSTM2 inhibits invasion, migration, and tumor formation of bladder cancer cells. The study also identified an SP1 site and several phytochemicals that can affect GSTM2 expression, with berberrubine and resveratrol being the most effective. Furthermore, it was found that a DNA methylation inhibitor and berberrubine can influence the methylation level of the GSTM2 gene.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Agriculture, Multidisciplinary
Jia-Nan Liu, Jing-Hui Xi, Zhun Wang, Shi-Wen Zhao, Xiao Wang, Yun-Wei Bu, Ke-Xin Zhou, Yu Pan, Shang Wang
Summary: In this study, 31 glutathione S-transferases (GSTs) were identified from the antennal transcriptome of Holotrichia parallela, with HpGSTd1 showing the highest expression level. Recombinant HpGSTd1 was found to have degradation activity towards various unsaturated aldehyde volatiles, and the metabolite of cinnamaldehyde was identified using high-resolution mass spectrometry (HRMS). Molecular docking analysis and site-directed mutagenesis revealed the key residues of HpGSTd1 involved in odorant degradation. The unsaturated aldehyde volatiles also elicited behavioral and electrophysiological responses in H. parallela. These findings suggest that HpGSTd1 plays an essential role in inactivating odorants in this underground pest, providing new insights for identifying molecular targets and exploring effective olfactory regulators.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Rosa A. Luirink, Marlies C. A. Verkade-Vreeker, Jan N. M. Commandeur, Daan P. Geerke
Article
Toxicology
Jan B. Koenderink, Jeroen J. M. W. van den Heuvel, Ab Bilos, Galvin Vredenburg, Nico P. E. Vermeulen, Frans G. M. Russel
ARCHIVES OF TOXICOLOGY
(2020)
Editorial Material
Biochemistry & Molecular Biology
Herman Autrup, Frank A. Barile, Sir Colin Berry, Bas J. Blaauboer, Alan Boobis, Herrmann Bolt, Christopher J. Borgert, Wolfgang Dekant, Daniel Dietrich, Jose L. Domingo, Gio Batta Gori, Helmut Greim, Jan Hengstler, Sam Kacew, Hans Marquardt, Olavi Pelkonen, Kai Savolainen, Pat Heslop-Harrison, Nico P. Vermeulen
CHEMICO-BIOLOGICAL INTERACTIONS
(2020)
Editorial Material
Toxicology
Herman Autrup, Frank A. Barile, Sir Colin Berry, Bas J. Blaauboer, Alan Boobis, Herrmann Bolt, Christopher J. Borgert, Wolfgang Dekant, Daniel Dietrich, Jose L. Domingo, Gio Batta Gori, Helmut Greim, Jan Hengstler, Sam Kacew, Hans Marquardt, Olavi Pelkonen, Kai Savolainen, Pat Heslop-Harrison, Nico P. Vermeulen
TOXICOLOGY IN VITRO
(2020)
Editorial Material
Toxicology
Herman Autrup, Frank A. Barile, Sir Colin Berry, Bas J. Blaauboer, Alan Boobis, Herrmann Bolt, Christopher J. Borgert, Wolfgang Dekant, Daniel Dietrich, Jose L. Domingo, Gio Batta Gori, Helmut Greim, Jan Hengstler, Sam Kacew, Hans Marquardt, Olavi Pelkonen, Kai Savolainen, Pat Heslop-Harrison, Nico P. Vermeulen
TOXICOLOGY LETTERS
(2020)
Editorial Material
Toxicology
Herman Autrup, Frank A. Barile, Sir Colin Berry, Bas J. Blaauboer, Alan Boobis, Herrmann Bolt, Christopher J. Borgert, Wolfgang Dekant, Daniel Dietrich, Jose L. Domingo, Gio Batta Gori, Helmut Greim, Jan Hengstler, Sam Kacew, Hans Marquardt, Olavi Pelkonen, Kai Savolainen, Pat Heslop-Harrison, Nico P. Vermeulen
ARCHIVES OF TOXICOLOGY
(2020)
Article
Pharmacology & Pharmacy
Sandra Ortega Ugalde, Kerstin Wallraven, Alexander Speer, Wilbert Bitter, Tom N. Grossmann, Jan N. M. Commandeur
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Paul Erhardt, Kenneth Bachmann, Donald Birkett, Michael Boberg, Nicholas Bodor, Gordon Gibson, David Hawkins, Gabrielle Hawksworth, Jack Hinson, Daniel Koehler, Brian Kress, Amarjit Luniwal, Hiroshi Masumoto, Raymond Novak, Phillip Portoghese, Jeffrey Sarver, M. Teresa Serafini, Christopher Trabbic, Nico Vermeulen, Steven Wrighton
Summary: This project, started over 15 years ago, aimed to create a glossary of drug metabolism terms specifically for medicinal chemists. Through extensive beta-testing, it engaged international audiences and expanded to enhance interdisciplinary communication. The current version maintains a chemical structure theme and tutorial comments to educate early stage researchers and investigators in today's collaborative drug discovery teams.
PURE AND APPLIED CHEMISTRY
(2021)
Correction
Toxicology
Liliana Capinha, Paul Jennings, Jan N. M. Commandeur
TOXICOLOGY LETTERS
(2021)
Article
Toxicology
Liliana Capinha, Paul Jennings, Jan N. M. Commandeur
Summary: The study demonstrates that three regioisomers of GSH-conjugates of TCE are formed in rat and human liver fractions, with different activities in human enzymes. Human liver fractions exhibit lower GSH-conjugation activity of TCE, suggesting a lower risk for TCE-associated nephrotoxic effects in humans compared to rats.
TOXICOLOGY LETTERS
(2021)
Letter
Toxicology
Frank A. Barile, Sir Colin Berry, Bas Blaauboer, Alan Boobis, Herrmann Bolt, Christopher J. Borgert, Wolfgang Dekant, Daniel Dietrich, Jose L. Domingo, Gio Batta Gori, Helmut Greim, Jan Hengstler, Sam Kacew, Hans Marquardt, Olavi Pelkonen, Kai Savolainen, Pat Heslop-Harrison, Aristides Tsatsakis, Nico P. Vermeulen
TOXICOLOGY LETTERS
(2021)
Letter
Toxicology
Frank A. Barile, Colin Berry, Bas Blaauboer, Alan Boobis, Herrmann M. Bolt, Christopher Borgert, Wolfgang Dekant, Daniel Dietrich, Jose L. Domingo, Corrado L. Galli, Gio Batta Gori, Helmut Greim, Jan G. Hengstler, Pat Heslop-Harrison, Sam Kacew, Hans Marquardt, Angela Mally, Olavi Pelkonen, Kai Savolainen, Emanuela Testai, Aristides Tsatsakis, Nico P. Vermeulen
Summary: The EU chemicals strategy for sustainability emphasizes the need for further regulation due to threats to human health and the environment. Concerns were raised by the German BfR regarding the scientific justification for this strategy. Any initiatives to improve consumer protection must be based on scientific knowledge and avoid confounding risk with other factors in policy-making.
ARCHIVES OF TOXICOLOGY
(2021)
Correction
Toxicology
Frank A. Barile, Sir Colin Berry, Bas Blaauboer, Alan Boobis, Herrmann Bolt, Christopher J. Borgert, Wolfgang Dekant, Daniel Dietrich, Jose L. Domingo, Gio Batta Gori, Helmut Greim, Jan Hengstler, Sam Kacew, Hans Marquardt, Olavi Pelkonen, Kai Savolainen, Pat Heslop-Harrison, Aristides Tsatsakis, Nico P. Vermeulen
TOXICOLOGY LETTERS
(2021)
Article
Toxicology
Liliana Capinha, Yaran Zhang, Anna-Katharina Holzer, Anna-Katharina Uckert, Melinda Zana, Giada Carta, Cormac Murphy, Jenna Baldovini, Zahra Mazidi, Johannes Grillari, Andras Dinnyes, Bob van de Water, Marcel Leist, Jan N. M. Commandeur, Paul Jennings
Summary: This study used six human derived cell models to investigate the effects of trichloroethylene (TCE) S-conjugates. The results showed that TCE S-conjugates induced Nrf2 pathway and unfolded protein response, which may explain the nephrotoxicity and neurotoxicity of TCE. This study expands our knowledge on the tissue specificity of TCE S-conjugates and highlights the value of human cell models and transcriptomics in mechanistic studies.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Toxicology
Liliana Capinha, Paul Jennings, Jan N. M. Commandeur
Summary: Bioactivation of trichloroethylene (TCE) through glutathione conjugation can cause adverse effects in the kidney and other extrahepatic tissues. Among the three regioisomeric conjugates formed, only 1,2-trans-DCVG and its corresponding cysteine-conjugate have been extensively studied. This study investigates the metabolism and cellular effects of 1,2-cis-DCVG and 1,2-cis-DCVC for the first time, revealing lower toxicity and slower formation rate for 1,2-cis-DCVC compared to 1,2-trans-DCVC.
TOXICOLOGY LETTERS
(2023)