Solid-Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution?

Stereodivergent Evolution of Artificial Enzymes for the Michael Reaction

(2018) Xavier Garrabou et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Directed Evolution of Protein Catalysts

(2018) Cathleen Zeymer et al.   Annual Review of Biochemistry

Enzymatic construction of highly strained carbocycles

(2018) Kai Chen et al.   SCIENCE

Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform

(2018) Fuqiang Ma et al.   Nature Communications

P450-Catalyzed Regio- and Diastereoselective Steroid Hydroxylation: Efficient Directed Evolution Enabled by Mutability Landscaping

(2018) Carlos G. Acevedo-Rocha et al.   ACS Catalysis

“Site and Mutation”-Specific Predictions Enable Minimal Directed Evolution Libraries

(2018) Jeffrey C Moore et al.   ACS Synthetic Biology

Relaxation of nonproductive binding and increased rate of coenzyme release in an alcohol dehydrogenase increases turnover with a nonpreferred alcohol enantiomer

(2017) Emil Hamnevik et al.   FEBS Journal

Structural and Computational Insight into the Catalytic Mechanism of Limonene Epoxide Hydrolase Mutants in Stereoselective Transformations

(2017) Zhoutong Sun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enantioselective, intermolecular benzylic C–H amination catalysed by an engineered iron-haem enzyme

(2017) Christopher K. Prier et al.   Nature Chemistry

Structure-guided SCHEMA recombination generates diverse chimeric channelrhodopsins

(2017) Claire N. Bedbrook et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Site-directed mutant libraries for isolating minimal mutations yielding functional changes

(2017) Dong hee Chung et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Discovery, Engineering, and Synthetic Application of Transaminase Biocatalysts

(2017) Iustina Slabu et al.   ACS Catalysis

Are Directed Evolution Approaches Efficient in Exploring Nature’s Potential to Stabilize a Lipase in Organic Cosolvents?

(2017) Ulrich Markel et al.   Catalysts

Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

(2017) Xue-Qing Li et al.   AMB Express

Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution

(2016) Aitao Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Rational design of thermostability in bacterial 1,3-1,4-β-glucanases through spatial compartmentalization of mutational hotspots

(2016) Chengtuo Niu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Fully automatized high-throughput enzyme library screening using a robotic platform

(2016) Mark Dörr et al.   BIOTECHNOLOGY AND BIOENGINEERING

New Concepts for Increasing the Efficiency in Directed Evolution of Stereoselective Enzymes

(2016) Zhoutong Sun et al.   CHEMISTRY-A EUROPEAN JOURNAL

Directed evolution of artificial metalloenzymes for in vivo metathesis

(2016) Markus Jeschek et al.   NATURE

Structure-Guided Triple-Code Saturation Mutagenesis: Efficient Tuning of the Stereoselectivity of an Epoxide Hydrolase

(2016) Zhoutong Sun et al.   ACS Catalysis

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently

(2015) Andrew Currin et al.   CHEMICAL SOCIETY REVIEWS

Improving and repurposing biocatalysts via directed evolution

(2015) Carl A Denard et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering

(2015) Matthew C. Blackburn et al.   NUCLEIC ACIDS RESEARCH

Biocatalysis: A Status Report

(2015) Andreas S. Bommarius   Annual Review of Chemical and Biomolecular Engineering

Computational tools for designing and engineering enzymes

(2014) Jiri Damborsky et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Directed evolution of stereoselective enzymes based on genetic selection as opposed to screening systems

(2014) Carlos G. Acevedo-Rocha et al.   JOURNAL OF BIOTECHNOLOGY

Computationally designed libraries for rapid enzyme stabilization

(2014) H. J. Wijma et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Speeding up Directed Evolution: Combining the Advantages of Solid-Phase Combinatorial Gene Synthesis with Statistically Guided Reduction of Screening Effort

(2014) Sabrina Hoebenreich et al.   ACS Synthetic Biology

Strategies for the discovery and engineering of enzymes for biocatalysis

(2013) Timo Davids et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Library construction and evaluation for site saturation mutagenesis

(2013) Bradford Sullivan et al.   ENZYME AND MICROBIAL TECHNOLOGY

Humanization of Antibodies Using Heavy Chain Complementarity-determining Region 3 Grafting Coupled within VitroSomatic Hypermutation

(2013) Peter M. Bowers et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Composability of regulatory sequences controlling transcription and translation inEscherichia coli

(2013) Sriram Kosuri et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-resolution mapping, characterization, and optimization of autonomously replicating sequences in yeast

(2012) I. Liachko et al.   GENOME RESEARCH

Combinatorial Design of an Anticalin Directed against the Extra-Domain B for the Specific Targeting of Oncofetal Fibronectin

(2012) Michaela Gebauer et al.   JOURNAL OF MOLECULAR BIOLOGY

Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay

(2012) Alexandre Melnikov et al.   NATURE BIOTECHNOLOGY

Inferring gene regulatory logic from high-throughput measurements of thousands of systematically designed promoters

(2012) Eilon Sharon et al.   NATURE BIOTECHNOLOGY

Complex effects of nucleotide variants in a mammalian cis-regulatory element

(2012) J. C. Kwasnieski et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Combination of In Vivo Selection and Cell Sorting for the Identification of Enantioselective Biocatalysts

(2011) Elena Fernández-Álvaro et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

When Second Best Is Good Enough: Another Probabilistic Look at Saturation Mutagenesis

(2011) Yuval Nov   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Synthetic Antibodies Designed on Natural Sequence Landscapes

(2011) Wenwu Zhai et al.   JOURNAL OF MOLECULAR BIOLOGY

Parallel on-chip gene synthesis and application to optimization of protein expression

(2011) Jiayuan Quan et al.   NATURE BIOTECHNOLOGY

Regio- and stereoselectivity of P450-catalysed hydroxylation of steroids controlled by laboratory evolution

(2011) Sabrina Kille et al.   Nature Chemistry

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering of an Orthogonal Aminoacyl-tRNA Synthetase for Efficient Incorporation of the Non-natural Amino Acid O-Methyl-L-tyrosine using Fluorescence-based Bacterial Cell Sorting

(2010) Sebastian M. Kuhn et al.   JOURNAL OF MOLECULAR BIOLOGY

Rational assignment of key motifs for function guides in silico enzyme identification

(2010) Matthias Höhne et al.   Nature Chemical Biology

Induced allostery in the directed evolution of an enantioselective Baeyer-Villiger monooxygenase

(2010) S. Wu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Directed Evolution of an Enantioselective Enoate-Reductase: Testing the Utility of Iterative Saturation Mutagenesis

(2009) Despina J. Bougioukou et al.   ADVANCED SYNTHESIS & CATALYSIS

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis

(2009) Rupali P Patwardhan et al.   NATURE BIOTECHNOLOGY

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

(2008) Joaquin Sanchis et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A novel solid phase technology for high-throughput gene synthesis

(2008) Jan Van den Brulle et al.   BIOTECHNIQUES

Enzyme optimization: moving from blind evolution to statistical exploration of sequence–function space

(2008) Richard J. Fox et al.   TRENDS IN BIOTECHNOLOGY

Non-polymerase-cycling-assembly-based chemical gene synthesis: Strategies, methods, and progress

(2007) Ai-Sheng Xiong et al.   BIOTECHNOLOGY ADVANCES