Review
Cell Biology
Ian James Bell, Matthew Sheldon Horn, Terence John Van Raay
Summary: The non-canonical Wnt signaling pathways have different roles in cancer and development, involving calcium and Vangl2. Cells tend to choose non-canonical Wnt signaling over canonical Wnt signaling when presented with a choice, but this occurs rarely in vivo.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Xiaowen Wu, Xiaochen Wang, Liying Shan, Jie Zhou, Xin Zhang, Endong Zhu, Hairui Yuan, Baoli Wang
Summary: The loss of NFIX gene affects endochondral ossification and mineralization in bone, with NFIX inducing osteoblast differentiation and impairing adipocyte formation by regulating HMGA1 expression. HMGA1 plays a role in osteoblast commitment and mediates NFIX function by activating canonical Wnt signaling pathways.
Article
Biochemistry & Molecular Biology
Zheng Ma, Yun Chen, Ju Qiu, Rui Guo, Keli Cai, Yan Zheng, Yuyao Zhang, Xueqing Li, Linsen Zan, Anning Li
Summary: In this study, a novel circRNA, circBTBD7, was identified to inhibit adipogenesis in bovine adipocytes by binding to miR-183 and regulating SMAD4 expression.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Fatih Karadeniz, Jung Hwan Oh, Hyun Jin Jo, Jiho Yang, Hyunjung Lee, Youngwan Seo, Chang-Suk Kong
Summary: This study reported the anti-adipogenic effect of dracunculin (DCC) in human bone marrow-derived mesenchymal stromal cells (hBM-MSCs). DCC inhibits lipid accumulation and the expression of adipogenic transcription factors. The mechanism of action involves activating the beta-catenin pathway via AMPK. DCC has potential use as a nutraceutical against bone marrow adiposity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Chatvadee Kornsuthisopon, Ajjima Chansaenroj, Jeeranan Manokawinchoke, Kevin A. Tompkins, Nopadon Pirarat, Thanaphum Osathanon
Summary: This study found that Jagged1 promoted osteo/odontogenic differentiation of human dental pulp stem cells (hDPSCs) by modulating the non-canonical Wnt pathway.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Chongyang Wang, Ruochen Hu, Liuyuan Duan, Qili Hou, Mengqing Yang, Ting Wang, Haijin Liu, Sa Xiao, Ruyi Dang, Juan Wang, Xinglong Wang, Shuxia Zhang, Zengqi Yang
Summary: This study showed that pseudorabies virus (PRV) infection activates the Wnt/beta-catenin signaling pathway, leading to enhanced virus proliferation and regulation of virus-induced autophagy. Inhibitors of this pathway can reduce PRV titers, while stimulation of the pathway can enhance PRV proliferation. These findings provide potential targets for the development of antiviral agents against PRV.
VETERINARY MICROBIOLOGY
(2022)
Article
Peripheral Vascular Disease
Irena Kasacka, Zaneta Piotrowska, Natalia Domian, Magdalena Acewicz, Alicja Lewandowska
Summary: The study revealed alterations in Wnt/beta-catenin signaling in the kidneys of rats during hypertension, with the severity of these changes depending on the type of hypertension. This study is the first to assess the levels of some elements of the canonical Wnt/beta-catenin signal transduction pathway in various types of arterial hypertension by immunohistochemistry, and may lead to further molecular and functional studies of this pathway in hypertension.
HYPERTENSION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Qi Zhou, Shengyu Lyu, Anthony A. Bertrand, Allison C. Hu, Candace H. Chan, Xiaoyan Ren, Marley J. Dewey, Aleczandria S. Tiffany, Brendan A. C. Harley, Justine C. Lee
Summary: By comparing noncrosslinked (NX-MC) and crosslinked (MC) forms of MC-GAG, this study evaluated the impact of stiffness on osteogenicity, finding that increasing stiffness can induce osteogenic differentiation through YAP/TAZ and the canonical Wnt signaling pathway, while activation of the canonical BMPR signaling pathway is independent of stiffness.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Feng Long, Xiaoyu Wang, Yuan Wan, Ziyi Zhang, Wenzhen Zhang, Linsen Zan, Gong Cheng
Summary: This study reveals the role of bta-miR-493 in regulating the proliferation and differentiation of bovine preadipocytes through targeting BMPR1A via the TGFfi/BMP and p38MAPK signaling pathways. This finding has significant implications for the molecular breeding of beef cattle, particularly in enhancing intramuscular fat deposition.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Cell Biology
Jiaqi Guo, Linglin Qian, Jingjing Ji, Zhenjun Ji, Yu Jiang, Ya Wu, Ziwei Yang, Genshan Ma, Yuyu Yao
Summary: Serpina3c promotes adipogenesis and maintains normal fat function by inhibiting the Wnt/beta-catenin pathway. Serpina3c knockout mice exhibit reduced body weight and white adipose tissue weight, and increased lipid depositions in the liver and muscles. In addition, knockout mice show severe insulin resistance.
CELLULAR SIGNALLING
(2022)
Article
Plant Sciences
Yingxing Xu, Yaping Jiang, Bin Jia, Yingzhen Wang, Tao Li
Summary: The study demonstrates that ICA can promote osteogenic differentiation and inhibit adipogenic differentiation of hBMSC through miR-23a-mediated activation of the Wnt/beta-catenin signaling pathway. This provides a new research direction for the application of ICA in bone tissue engineering.
Article
Medicine, Research & Experimental
Jian Song, Nan Jiang, Xueqi Gan, Wei Zhi, Zhuoli Zhu
Summary: The study demonstrated that the local injection of argatroban can rescue bone loss in periodontal disease, while inducing osteogenic differentiation through activating the canonical Wnt signaling pathway. Therefore, argatroban has potential applications in promoting alveolar bone regeneration and remodeling based on BMSCs.
Article
Endocrinology & Metabolism
Bo Wang, Saima Khan, Pengtao Wang, Xiaofang Wang, Yangxi Liu, Jingjing Chen, Xiaolin Tu
Summary: The small molecule CHIR99021 (C91) has been found to promote osteoblast differentiation and mineralization by activating the canonical Wnt signaling pathway and inducing autophagy. In addition, a C91 slow-release hydrogel has been developed for in vivo detection of C91 in the osteogenesis process.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Cell Biology
Florian Hermans, Lara Hemeryck, Ivo Lambrichts, Annelies Bronckaers, Hugo Vankelecom
Summary: Tooth development relies on interactions between dental epithelium and mesenchyme, regulated by molecular pathways including Wnt, BMP, FGF, and Shh. These signaling modules intertwine during tooth development, outlining specific cell populations' development pathways.Recent studies have uncovered crucial Wnt-Shh interactions during odontogenesis, showing conservation in developing ectodermal appendages.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Morgane Le Rolle, Filippo Massa, Pam Siggers, Laurent Turchi, Agnes Loubat, Bon-Kyoung Koo, Hans Clevers, Andy Greenfield, Andreas Schedl, Marie-Christine Chaboissier, Anne-Amandine Chassot
Summary: This study shows that germ cell-intrinsic beta-catenin activity in mouse embryonic ovaries is essential for maintaining pluripotency and its repression is crucial for differentiation and meiosis entry. The interaction of beta-catenin with the pluripotent-associated factor POU5F1 in the nucleus is associated with germ cell pluripotency, and its exit from the nucleus correlates with germ cell differentiation. These findings highlight the role of beta-catenin as a central gatekeeper in ovarian differentiation and gametogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Ke Mi, Lizhong Zeng, Yang Chen, Jingya Ning, Siyuan Zhang, Peilin Zhao, Shuanying Yang
Summary: In this study, the researchers explored the role of DHX38 in NSCLC and its underlying molecular mechanism. They found that DHX38 was overexpressed in NSCLC and patients with high DHX38 expression had poor prognosis. DHX38 promoted cell proliferation, migration, and invasion in NSCLC and activated the MAPK pathway. The researchers also identified G3BP1 as a target protein that interacted with DHX38 and showed that DHX38 regulated the expression of G3BP1. Silencing G3BP1 reversed the effects of DHX38 overexpression on tumor cell proliferation, migration, and invasion and inhibited the MAPK pathway activation.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Tiina A. Jokela, Mark A. Dane, Rebecca L. Smith, Kaylyn L. Devlin, Sundus Shalabi, Jennifer C. Lopez, Masaru Miyano, Martha R. Stampfer, James E. Korkola, Joe W. Gray, Laura M. Heiser, Mark A. Labarge
Summary: Microenvironment signals have a significant impact on cell fate and tissue homeostasis. Understanding how different microenvironment factors regulate cellular phenotype has been challenging. In this study, a high-throughput microenvironment microarray was used to identify factors that support the proliferation and maintenance of primary human mammary luminal epithelial cells. Multiple factors that modulate luminal cell number were identified and their effects were confirmed using RNA sequencing and cell-based functional studies. Hepatocyte growth factor (HGF) was found to be robust to individual variation and played a role in expanding luminal cells. Our approach demonstrates the power of high-dimensional cell-based approaches in dissecting microenvironmental signals.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chao He, Yongfeng Ding, Yan Yang, Gang Che, Fei Teng, Haohao Wang, Jing Zhang, Donghui Zhou, Yanyan Chen, Zhan Zhou, Haiyong Wang, Lisong Teng
Summary: This study categorized gastric cancer patients into three stemness subtypes, each demonstrating distinct prognoses, components of tumor microenvironment (TME) infiltration, and varying sensitivity or resistance to treatment. A stemness risk model was constructed to predict treatment response and prognosis.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haile Zhao, Lijuan Feng, Rui Cheng, Man Wu, Xiaozhou Bai, Lifei Fan, Yaping Liu
Summary: miR-29c-3p is overexpressed in benign and malignant ovarian carcinoma and is associated with poor prognosis. Its overexpression modulates tumorigenesis in ovarian cancer cells, including epithelial-mesenchymal transition, proliferation, migration, and invasion, through the regulation of DNMT3A, TET1, and HBP1. miR-29c-3p may serve as a potential biomarker for clinical diagnosis or co-diagnosis of ovarian carcinoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haiyan Zhao, Fangfang Bi, Mengyuan Li, Yuhan Diao, Chen Zhang
Summary: This study confirmed the tumor suppressor effect of RNF180 on ovarian cancer, elucidated the mechanism of the molecule network related to RNF180 and IPO4 in ovarian cancer, and identified a new therapeutic target for ovarian cancer.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chu Chen, Guanhua Xu, Jiajia Chen, Chunshuai Wu, Jinlong Zhang, Jiawei Jiang, Hongxiang Hong, Zhiming Cui
Summary: This study investigated the role of transcription factor FoxO1 in facet joint osteoarthritis (FJOA) and found that FoxO1 deletion led to severe osteoarthritic changes. Transcriptome sequencing and bioinformatics analysis identified differentially expressed genes (DEGs) and potential key contributors to FJOA. Additionally, over-expression of certain genes and inhibition of others were shown to counteract the impairments caused by FoxO1 deletion in chondrocyte migration and extracellular matrix synthesis. These findings help unravel the molecular mechanisms underlying FJOA and open up promising therapeutic avenues for its treatment.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Wen Deng, Ru Chen, Situ Xiong, Jianqiang Nie, Hailang Yang, Ming Jiang, Bing Hu, Xiaoqiang Liu, Bin Fu
Summary: This study demonstrates that circFSCN1 is upregulated in bladder cancer and associated with cancer-specific survival. CircFSCN1 promotes tumor progression and epithelial-mesenchymal transition in bladder cancer through enhancing MDM2-mediated silencing of p53 by sponging miR-145-5p.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Jun Wu, Weibin Hu, Wenhui Yang, Yihao Long, Kaizhao Chen, Fugui Li, Xiaodong Ma, Xun Li
Summary: Cholesterol biosynthesis and metabolism play critical roles in tumor development and microenvironmental conditions. Squalene Epoxidase (SQLE), the second rate-limiting enzyme in cholesterol synthesis, is found to be uniquely expressed in various cancers, and its expression level is closely associated with tumor mutation burden and microsatellite instability. SQLE expression is negatively correlated with immune cell infiltration. Inhibition of SQLE alters the immune response in the tumor microenvironment. Furthermore, protein metabolism and translation are identified as main binding factors with SQLE.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhihong Zhang, Mingyue Li, Yi Tai, Yue Xing, Hongxiang Zuo, Xuejun Jin, Juan Ma
Summary: ZNF70 plays an important role in colitis-associated colorectal cancer (CAC) by regulating macrophages IL-1 beta secretion to promote HCT116 proliferation. It may serve as a promising target for treating CAC.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zenghong Wu, Gangping Li, Weijun Wang, Kun Zhang, Mengke Fan, Yu Jin, Rong Lin
Summary: This study comprehensively explored the role of immune checkpoints and tumor microenvironment in gastric cancer patients based on genomic data. It constructed an ICIs signature and ICI score to evaluate patient prognosis and heterogeneity.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Yantong Wan, Jieshu Zhou, Panpan Zhang, Xuemei Lin, Hao Li
Summary: This study found that Rac1 plays a role in astrocyte activation and attenuates chronic inflammatory pain by blocking the phosphorylation of NLRP3 inflammasome and NF-kappa B.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhen Wang, Diankun She, Lei Liu, Xianming Hua, Hao Zhu, Lingfeng Yu, Han Wang, Yan Zhu, Gentao Fan, Yicun Wang, Meng Xu, Guangxin Zhou
Summary: Circular RNAs (circRNAs) are non-coding RNAs that play a role in the regulation of various cancers, including osteosarcoma (OS). This study identified circSATB2 as a highly expressed circRNA in OS tissues and cell lines, and demonstrated its involvement in promoting OS proliferation and migration. Mechanistically, circSATB2 was found to regulate the progression of OS by sponging miR-661 and FUS to regulate ZNFX1 mRNA. These findings suggest that circSATB2 could serve as a prognostic marker and therapeutic target for osteosarcoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Kenichi Ogata, Masafumi Moriyama, Tatsuya Kawado, Hiroki Yoshioka, Aiko Yano, Mayu Matsumura-Kawashima, Seiji Nakamura, Shintaro Kawano
Summary: This study found that extracellular vesicles released by induced pluripotent stem cells can reduce inflammatory cell infiltration, increase saliva volume, and decrease the production of antibodies associated with Sjogren's syndrome in a mouse model. The let-7 family in these vesicles may suppress the expression of TLR4 and NF-kappa B, which leads to the inhibition of pro-inflammatory cytokine production through the MAPK pathway.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Mikayla R. Erdelsky, Sarah A. Groves, Charmi Shah, Samantha B. Delios, M. Bibiana Umana, Donald H. Maurice
Summary: Recent evidence suggests that cAMP signaling within the primary cilium plays a crucial role in promoting adipogenic differentiation of 3T3-L1 preadipocytes. In this study, the researchers identified the specific cAMP phosphodiesterases expressed by these cells and found that inhibition of PDE4 promotes FFAR4-mediated adipogenesis. This work could potentially lead to the discovery of more targeted therapeutic approaches for controlling adipogenesis and differentiation of other stem cells.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chun-Hui Liu, Jun-Jie Zhang, Qian-Jin Zhang, Yang Dong, Zhen-Duo Shi, Si-Hao Hong, Hou-Guang He, Wei Wu, Cong-Hui Han, Lin Hao
Summary: Bladder cancer, the most common malignant tumor in the urinary system, is associated with significantly up-regulated expression of P3H4, which is regulated by METTL3 and plays a crucial role in the proliferation, metastasis, and EMT progression of bladder cancer. Targeting this METTL3-P3H4 pathway may serve as a potential therapeutic strategy for bladder cancer.
CELLULAR SIGNALLING
(2024)