Article
Engineering, Biomedical
Y. Kawamura, S. -I. Murtada, F. Gao, X. Liu, G. Tellides, J. D. Humphrey
Summary: Altered TGFβ signaling increases the risk of aortic dissection in patients, affecting medial properties and adventitial strength. This study emphasizes the importance of cell- and layer-specific studies to understand changes in wall biology that dictate differences between life and death in cases of thoracic aortic dissection and rupture.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Ming-Hui Zheng, Su-Kang Shan, Xiao Lin, Feng Xu, Feng Wu, Bei Guo, Fu-Xing-zi Li, Zhi-Ang Zhou, Yi Wang, Li-Min Lei, Ke-Xin Tang, Jia-Yue Duan, Yun-Yun Wu, Ye-Chi Cao, Xiao-Bo Liao, Ling-Qing Yuan
Summary: Vascular calcification is common in chronic renal failure (CRF) patients and significantly increases the risk of cardiovascular events. Previous studies have found that endothelial cells (ECs) and vascular smooth muscle cells (VSMCs) can interact and regulate VSMCs calcification through paracrine effects. This study aims to investigate the effects of exosomes secreted by high phosphorus-induced adventitial fibroblasts (AFs) on VSMCs calcification and the underlying mechanism, further highlighting the importance of AFs in the high phosphorus vascular wall microenvironment.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Medicine, Research & Experimental
Chao Ye, Fen Zheng, Tao Xu, Nan Wu, Ying Tong, Xiao-Qing Xiong, Ye-Bo Zhou, Jue-Jin Wang, Qi Chen, Yue-Hua Li, Guo-Qing Zhu, Ying Han
Summary: This study reveals that NE promotes the release of small EVs and ACE transfer from AFs, leading to VSMCs proliferation in hypertension. Intervention of AFs-derived EVs release may be a potential therapeutic approach for vascular remodeling in hypertension related to excessive sympathetic activation.
Article
Physiology
Amber M. Kennon, James A. Stewart
Summary: By studying different cell types involved in diabetes-mediated vascular calcification, it was found that VSMCs depend on the AGE/RAGE pathway, while AFBs exhibit a calcification response that is not mediated by AGE. Additionally, the activation of P38 MAPK signaling pathway plays a crucial role in this process.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Immunology
Yuhan Chen, Yuanyuan Chen, Xueze Jiang, Mengkun Shi, Zhenwei Yang, Zhiyong Chen, Xuesheng Hua, Jie Chen, Yuepeng Wang
Summary: This study demonstrated that FGF10 derived from VAFs promoted the proliferation, migration, and neointima formation of VSMCs, while the FGF10-FGFR2 signaling pathway triggered the activation of the MAPK/PI3K-AKT pathways in VSMCs. Additionally, PDGF synergistically amplified the FGF10 signaling.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Endocrinology & Metabolism
Stephanie K. Bonney, Liam T. Sullivan, Timothy J. Cherry, Richard Daneman, Andy Y. Shih
Summary: The study identified the distribution and characteristics of perivascular fibroblasts (PVFs) in the cerebral cortex, providing clarity on their differences with mural cells and addressing the challenge of distinguishing between the two cell types.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Amber M. Kennon, James A. A. Stewart Jr
Summary: Our study aimed to investigate the impact of paracrine signals from different aortic layers on the diabetic microenvironment, specifically on vascular smooth muscle cells (VSMCs) and adventitial fibroblasts (AFBs). We collected pre-conditioned calcified media from diabetic and non-diabetic VSMCs and AFBs to treat murine diabetic and non-diabetic VSMCs and AFBs. Calcium assays, western blots, and cytokine/chemokine profile kits were used to determine signaling responses. The results showed that responses from VSMCs and AFBs differed when treated with non-diabetic and diabetic pre-conditioned media.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Joana Alpoim-Moreira, Anna Szostek-Mioduchowska, Magda Slyszewska, Maria Rosa Rebordao, Dariusz J. Skarzynski, Graca Ferreira-Dias
Summary: Endometrosis is a major cause of infertility in mares, characterized by excessive deposition of collagen and alpha smooth muscle actin in the endometrium. Epigenetic alterations in fibroblast phenotype are linked with the development of endometrial fibrosis. This study investigated the in vitro epigenetic regulation of mare endometrial fibrogenesis through DNMTs transcription and the effect of 5-aza-2'-deoxycytidine on collagen expression in fibroblasts challenged with TGF-beta 1. The findings suggest the potential role of epigenetic mechanisms in equine endometrial fibrogenesis and highlight the therapeutic potential of 5-aza-dC.
Article
Cardiac & Cardiovascular Systems
Alessandro Bressan, Maristella Donato, Paola Canzano, Marina Camera, Paolo Poggio, Mariangela Garofalo, Giovanni Panighel, Massimiliano Ruscica, Andrea Baragetti, Valentina Bollati, Elisabetta Faggin, Marcello Rattazzi, Alberico L. Catapano, Nicola Ferri, Maria Giovanna Lupo, Maria Francesca Greco, Sara De Martin
Summary: This study investigated the influence of kidney function on PCSK9 levels and its role in vascular smooth muscle cell (SMC) calcification. The results showed a negative association between glomerular filtration rate and plasma PCSK9 levels. Overexpression of PCSK9 in cells led to increased calcification and release of extracellular vesicles enriched in calcium and alkaline phosphatase. The findings suggest a direct role of PCSK9 in vascular calcification associated with renal impairment.
Article
Cell & Tissue Engineering
Qing Zhang, Ting Chen, Yun Zhang, Lingxia Lyu, Bohuan Zhang, Chengchen Huang, Xuhao Zhou, Yutao Wu, Zhoubin Li
Summary: The expression of miR-30c-5p was significantly decreased in atherosclerotic arteries, and it inhibited VSMC differentiation from adventitial Sca-1(+) progenitor cells through targeting OPG, suggesting a new therapeutic target for VSMC-associated diseases.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Allen Sam Titus, Mereena George Ushakumary, Harikrishnan Venugopal, Mingyi Wang, Edward G. Lakatta, Shivakumar Kailasam
Summary: The molecular mechanisms underlying the therapeutic effects of metformin, a drug used to treat diabetes, beyond its anti-hyperglycemic effects are not well understood. Recent investigations have shown that a receptor called Discoidin Domain Receptor 2 (DDR2) plays a role in collagen type I gene expression in fibroblasts and may be involved in arterial fibrosis and metabolic syndrome. This study found that metformin targets DDR2 and inhibits the expression of fibronectin and collagen type I, providing insight into the molecular basis of its protective effects against vascular and cardiac fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dermatology
Lukas Nollet, Matthias Van Gils, Andy Willaert, Paul J. Coucke, Olivier M. Vanakker
Summary: Excessive DDR/PARP1 signaling is involved in the pathogenesis of PXE, contributing to aberrant mineralization. Treatment with the PARP1 inhibitor minocycline attenuates this signaling and reduces ectopic calcification. This study provides new insights for the treatment of PXE.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Multidisciplinary Sciences
Bart Spronck, Marcos Latorre, Mo Wang, Sameet Mehta, Alexander W. Caulk, Pengwei Ren, Abhay B. Ramachandra, Sae-Il Murtada, Alexia Rojas, Chang-Shun He, Bo Jiang, Matthew R. Bersi, George Tellides, Jay D. Humphrey
Summary: The study found that hypertension-induced aortic remodeling may be adaptive or maladaptive, depending on different immuno-mechanical mechanisms. The aortas of 129S6/SvEvTac mice showed near mechano-adaptive remodeling, while those of C57BL/6 J mice exhibited grossly maladaptive remodeling.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2021)
Review
Pharmacology & Pharmacy
Shigang Lin, Ruoran Lin, Hongkun Zhang, Qingbo Xu, Yangyan He
Summary: This review focuses on the functional changes of peripheral arterial cells and the mechanisms of PAD pathogenesis. It summarizes the progress of current clinical treatment and potential therapeutic methods for PAD, and provides insights for future perspectives.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Oncology
Bowen Li, Zhiwei Wang, Junmou Hong, Yanjia Che, Ruoshi Chen, Zhipeng Hu, Xiaoping Hu, Qi Wu, Junxia Hu, Min Zhang
Summary: The study revealed that patients with AMD had iron deficiency, and this deficiency was associated with the development of AMD in hypertensive patients. Experiments on mice showed that iron-deficient feeding combined with Ang II injection promoted the formation of AMD and significantly shortened the survival time of mice. Additionally, iron deficiency significantly impaired the cytoskeleton of vascular smooth muscle cells.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Geriatrics & Gerontology
Odessa Addison, Alice S. Ryan, Steven J. Prior, Leslie I. Katzel, Rishi Kundi, Brajesh K. Lal, Andrew W. Gardner
JOURNAL OF GERIATRIC PHYSICAL THERAPY
(2017)
Article
Rehabilitation
Odessa Addison, Steven J. Prior, Rishi Kundi, Monica C. Serra, Leslie I. Katzel, Andrew W. Gardner, Alice S. Ryan
ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION
(2018)
Article
Surgery
Behzad S. Farivar, Shahab Toursavadkohi, Thomas S. Monahan, Jashank Sharma, Areck A. Ucuzian, Rishi Kundi, Rajabrata Sarkar, Brajesh K. Lal
JOURNAL OF VASCULAR SURGERY-VENOUS AND LYMPHATIC DISORDERS
(2019)
Article
Clinical Neurology
Abhinandan Reddy Mallepally, Yelamarthy Karthik, Neshar Ansari, Harvinder Singh Chhabra, Shakti Amar Goel
WORLD NEUROSURGERY
(2019)
Article
Critical Care Medicine
Leonid Belyayev, Joseph A. Herrold, Ara Ko, Rishi Kundi, Joseph J. DuBose, Thomas M. Scalea, Jonathan J. Morrison
JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
(2020)
Article
Emergency Medicine
Marta J. Madurska, Ashley McLenithan, Thomas M. Scalea, Rishi Kundi, Joseph M. White, Jonathan J. Morrison, Joseph J. DuBose
Summary: The study compared the outcomes of P-REBOA and C-REBOA in trauma patients with infradiaphragmatic hemorrhage, finding that prolonged REBOA occlusion time was associated with higher mortality and organ failure. P-REBOA showed a potential advantage in reducing organ support requirements, but its survival benefit in prolonged occlusion remains unclear. More clinical data are needed to further elucidate the benefits of partial occlusion REBOA.
EUROPEAN JOURNAL OF TRAUMA AND EMERGENCY SURGERY
(2022)
Article
Emergency Medicine
Melike Harfouche, Jonathan Morrison, Rishi Kundi, Joseph J. DuBose, Thomas M. Scalea
JOURNAL OF ENDOVASCULAR RESUSCITATION AND TRAUMA MANAGEMENT
(2020)
Meeting Abstract
Surgery
Joseph Jeremy DuBose, Faris Azar, Rishi Kundi, Thomas M. Scalea, Tiffany Kay Bee, Timothy C. Fabian, David V. Feliciano
JOURNAL OF THE AMERICAN COLLEGE OF SURGEONS
(2020)
Meeting Abstract
Surgery
Matthew T. Chrencik, Steven J. Prior, Eric J. Christensen, Rishi Kundi, Alice S. Ryan, Odessa Addison, Brajesh K. Lal
JOURNAL OF VASCULAR SURGERY
(2020)
Article
Orthopedics
Abhinandan Reddy, Rajat Mahajan, Tarush Rustagi, Shakti A. Goel, Murari L. Bansal, Harvinder Singh Chhabra
ASIAN SPINE JOURNAL
(2019)
Letter
Emergency Medicine
Francisco Javier Martin-Sanchez, Berenice Nayla Brizzi, Sira Aguilo Mir, Montserrat Lazaro del Nogal
Article
Rehabilitation
Odessa Addison, Rishi Kundi, Alice S. Ryan, Andrew P. Goldberg, Richa Patel, Brajesh K. Lal, Steven J. Prior
DISABILITY AND REHABILITATION
(2018)
Review
Orthopedics
Shakti A. Goel, Hitesh N. Modi
INDIAN JOURNAL OF ORTHOPAEDICS
(2018)
Article
Surgery
Richa Kalsi, Charles B. Drucker, Jose H. Salazar, Lauren I. Luther, Jose J. Diaz, Rishi Kundi
JOURNAL OF VASCULAR SURGERY CASES AND INNOVATIVE TECHNIQUES
(2018)
Review
Anesthesiology
Bianca M. Conti, Justin E. Richards, Rishi Kundi, Jason Nascone, Thomas M. Scalea, Maureen McCunn
A & A CASE REPORTS
(2017)
Article
Cell Biology
Ke Mi, Lizhong Zeng, Yang Chen, Jingya Ning, Siyuan Zhang, Peilin Zhao, Shuanying Yang
Summary: In this study, the researchers explored the role of DHX38 in NSCLC and its underlying molecular mechanism. They found that DHX38 was overexpressed in NSCLC and patients with high DHX38 expression had poor prognosis. DHX38 promoted cell proliferation, migration, and invasion in NSCLC and activated the MAPK pathway. The researchers also identified G3BP1 as a target protein that interacted with DHX38 and showed that DHX38 regulated the expression of G3BP1. Silencing G3BP1 reversed the effects of DHX38 overexpression on tumor cell proliferation, migration, and invasion and inhibited the MAPK pathway activation.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Tiina A. Jokela, Mark A. Dane, Rebecca L. Smith, Kaylyn L. Devlin, Sundus Shalabi, Jennifer C. Lopez, Masaru Miyano, Martha R. Stampfer, James E. Korkola, Joe W. Gray, Laura M. Heiser, Mark A. Labarge
Summary: Microenvironment signals have a significant impact on cell fate and tissue homeostasis. Understanding how different microenvironment factors regulate cellular phenotype has been challenging. In this study, a high-throughput microenvironment microarray was used to identify factors that support the proliferation and maintenance of primary human mammary luminal epithelial cells. Multiple factors that modulate luminal cell number were identified and their effects were confirmed using RNA sequencing and cell-based functional studies. Hepatocyte growth factor (HGF) was found to be robust to individual variation and played a role in expanding luminal cells. Our approach demonstrates the power of high-dimensional cell-based approaches in dissecting microenvironmental signals.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chao He, Yongfeng Ding, Yan Yang, Gang Che, Fei Teng, Haohao Wang, Jing Zhang, Donghui Zhou, Yanyan Chen, Zhan Zhou, Haiyong Wang, Lisong Teng
Summary: This study categorized gastric cancer patients into three stemness subtypes, each demonstrating distinct prognoses, components of tumor microenvironment (TME) infiltration, and varying sensitivity or resistance to treatment. A stemness risk model was constructed to predict treatment response and prognosis.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haile Zhao, Lijuan Feng, Rui Cheng, Man Wu, Xiaozhou Bai, Lifei Fan, Yaping Liu
Summary: miR-29c-3p is overexpressed in benign and malignant ovarian carcinoma and is associated with poor prognosis. Its overexpression modulates tumorigenesis in ovarian cancer cells, including epithelial-mesenchymal transition, proliferation, migration, and invasion, through the regulation of DNMT3A, TET1, and HBP1. miR-29c-3p may serve as a potential biomarker for clinical diagnosis or co-diagnosis of ovarian carcinoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haiyan Zhao, Fangfang Bi, Mengyuan Li, Yuhan Diao, Chen Zhang
Summary: This study confirmed the tumor suppressor effect of RNF180 on ovarian cancer, elucidated the mechanism of the molecule network related to RNF180 and IPO4 in ovarian cancer, and identified a new therapeutic target for ovarian cancer.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chu Chen, Guanhua Xu, Jiajia Chen, Chunshuai Wu, Jinlong Zhang, Jiawei Jiang, Hongxiang Hong, Zhiming Cui
Summary: This study investigated the role of transcription factor FoxO1 in facet joint osteoarthritis (FJOA) and found that FoxO1 deletion led to severe osteoarthritic changes. Transcriptome sequencing and bioinformatics analysis identified differentially expressed genes (DEGs) and potential key contributors to FJOA. Additionally, over-expression of certain genes and inhibition of others were shown to counteract the impairments caused by FoxO1 deletion in chondrocyte migration and extracellular matrix synthesis. These findings help unravel the molecular mechanisms underlying FJOA and open up promising therapeutic avenues for its treatment.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Wen Deng, Ru Chen, Situ Xiong, Jianqiang Nie, Hailang Yang, Ming Jiang, Bing Hu, Xiaoqiang Liu, Bin Fu
Summary: This study demonstrates that circFSCN1 is upregulated in bladder cancer and associated with cancer-specific survival. CircFSCN1 promotes tumor progression and epithelial-mesenchymal transition in bladder cancer through enhancing MDM2-mediated silencing of p53 by sponging miR-145-5p.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Jun Wu, Weibin Hu, Wenhui Yang, Yihao Long, Kaizhao Chen, Fugui Li, Xiaodong Ma, Xun Li
Summary: Cholesterol biosynthesis and metabolism play critical roles in tumor development and microenvironmental conditions. Squalene Epoxidase (SQLE), the second rate-limiting enzyme in cholesterol synthesis, is found to be uniquely expressed in various cancers, and its expression level is closely associated with tumor mutation burden and microsatellite instability. SQLE expression is negatively correlated with immune cell infiltration. Inhibition of SQLE alters the immune response in the tumor microenvironment. Furthermore, protein metabolism and translation are identified as main binding factors with SQLE.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhihong Zhang, Mingyue Li, Yi Tai, Yue Xing, Hongxiang Zuo, Xuejun Jin, Juan Ma
Summary: ZNF70 plays an important role in colitis-associated colorectal cancer (CAC) by regulating macrophages IL-1 beta secretion to promote HCT116 proliferation. It may serve as a promising target for treating CAC.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zenghong Wu, Gangping Li, Weijun Wang, Kun Zhang, Mengke Fan, Yu Jin, Rong Lin
Summary: This study comprehensively explored the role of immune checkpoints and tumor microenvironment in gastric cancer patients based on genomic data. It constructed an ICIs signature and ICI score to evaluate patient prognosis and heterogeneity.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Yantong Wan, Jieshu Zhou, Panpan Zhang, Xuemei Lin, Hao Li
Summary: This study found that Rac1 plays a role in astrocyte activation and attenuates chronic inflammatory pain by blocking the phosphorylation of NLRP3 inflammasome and NF-kappa B.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhen Wang, Diankun She, Lei Liu, Xianming Hua, Hao Zhu, Lingfeng Yu, Han Wang, Yan Zhu, Gentao Fan, Yicun Wang, Meng Xu, Guangxin Zhou
Summary: Circular RNAs (circRNAs) are non-coding RNAs that play a role in the regulation of various cancers, including osteosarcoma (OS). This study identified circSATB2 as a highly expressed circRNA in OS tissues and cell lines, and demonstrated its involvement in promoting OS proliferation and migration. Mechanistically, circSATB2 was found to regulate the progression of OS by sponging miR-661 and FUS to regulate ZNFX1 mRNA. These findings suggest that circSATB2 could serve as a prognostic marker and therapeutic target for osteosarcoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Kenichi Ogata, Masafumi Moriyama, Tatsuya Kawado, Hiroki Yoshioka, Aiko Yano, Mayu Matsumura-Kawashima, Seiji Nakamura, Shintaro Kawano
Summary: This study found that extracellular vesicles released by induced pluripotent stem cells can reduce inflammatory cell infiltration, increase saliva volume, and decrease the production of antibodies associated with Sjogren's syndrome in a mouse model. The let-7 family in these vesicles may suppress the expression of TLR4 and NF-kappa B, which leads to the inhibition of pro-inflammatory cytokine production through the MAPK pathway.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Mikayla R. Erdelsky, Sarah A. Groves, Charmi Shah, Samantha B. Delios, M. Bibiana Umana, Donald H. Maurice
Summary: Recent evidence suggests that cAMP signaling within the primary cilium plays a crucial role in promoting adipogenic differentiation of 3T3-L1 preadipocytes. In this study, the researchers identified the specific cAMP phosphodiesterases expressed by these cells and found that inhibition of PDE4 promotes FFAR4-mediated adipogenesis. This work could potentially lead to the discovery of more targeted therapeutic approaches for controlling adipogenesis and differentiation of other stem cells.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chun-Hui Liu, Jun-Jie Zhang, Qian-Jin Zhang, Yang Dong, Zhen-Duo Shi, Si-Hao Hong, Hou-Guang He, Wei Wu, Cong-Hui Han, Lin Hao
Summary: Bladder cancer, the most common malignant tumor in the urinary system, is associated with significantly up-regulated expression of P3H4, which is regulated by METTL3 and plays a crucial role in the proliferation, metastasis, and EMT progression of bladder cancer. Targeting this METTL3-P3H4 pathway may serve as a potential therapeutic strategy for bladder cancer.
CELLULAR SIGNALLING
(2024)