Article
Biochemistry & Molecular Biology
Kamalakshi Deka, Sougata Saha
Summary: This study identified arginylation of HuR as a factor affecting its stability and RNA binding activity, leading to alternative polyadenylation of Hsp70.3 mRNA and playing a role in early heat stress response.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Biochemistry & Molecular Biology
Francesca Paron, Simone Barattucci, Sara Cappelli, Maurizio Romano, Christian Berlingieri, Cristiana Stuani, Douglas Laurents, Miguel Mompean, Emanuele Buratti
Summary: TDP-43 is a nucleic acid-binding protein that can accumulate in the cytoplasm under pathological conditions, leading to diseases like frontotemporal dementia and ALS. Research on TDP-43 is focusing on its post-translational modifications and their connection to disease-associated mutations. A recent study identified a novel mutation (S375G) in TDP-43 that affected nuclear-cytoplasmic distribution and cellular toxicity. The study also found that this mutation and its phosphomimetic variant (S375E) regulate distinct sets of genes but share a common target in mitochondrial apoptotic genes. These findings support the idea that alterations in TDP-43 post-translational modifications play a role in disease pathogenesis and are linked to mitochondrial health.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Ahmed Sidali, Varsha Teotia, Nadeen Shaikh Solaiman, Nahida Bashir, Radhakrishnan Kanagaraj, John J. Murphy, Kalpana Surendranath
Summary: This article reviews the role of AU-RBPs in maintaining genome integrity, including their regulation of mRNA transcripts and response to genotoxic stresses. Dysfunctional AU-RBPs have been found to be associated with many human cancers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Natalia Filippova, Xiuhua Yang, Subramaniam Ananthan, Jennifer Calano, Vibha Pathak, Larry Bratton, Rakesh H. Vekariya, Sixue Zhang, Edward Ofori, Emily N. Hayward, David Namkoong, David K. Crossman, Michael R. Crowley, Peter H. King, James Mobley, Louis B. Nabors
Summary: The study developed a new class of inhibitors targeting HuR protein dimerization, successfully inhibiting cancer cell proliferation, inducing apoptosis, and reducing colony formation, while also inhibiting tumor growth in mouse models. The research highlights the importance of focusing on key attributes of HuR, such as cytoplasmic localization and multimerization, which contribute to cancer progression.
Review
Oncology
Kun Xu, Shenghui Sun, Mingjing Yan, Ju Cui, Yao Yang, Wenlin Li, Xiuqing Huang, Lin Dou, Beidong Chen, Weiqing Tang, Ming Lan, Jian Li, Tao Shen
Summary: DEAD-box (DDX)5 and DDX17, members of the DEAD-box RNA helicase family, are shuttle proteins that can move between the nucleus and cytoplasm. They participate in various RNA metabolism processes and play important roles in tumorigenesis and tumor progression.
FRONTIERS IN ONCOLOGY
(2022)
Review
Cell Biology
Wiljan J. A. J. Hendriks, Remco T. P. van Cruchten, Rafael Pulido
Summary: Protein tyrosine phosphatases and protein tyrosine kinases play important roles in controlling molecular signaling processes at cellular and organismal levels, affecting the quality of life. This review focuses on studying germline variants in the genes encoding the thirty-seven classical members of the protein tyrosine phosphatase superfamily. The results suggest a potential association between individual genes and specific disorders, highlighting the need for further research to establish a comprehensive genotype-phenotype map for this intriguing protein family.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Oncology
Yu-Zhou Chang, Rui-Chao Chai, Bo Pang, Xin Chang, Song Yuan An, Ke-Nan Zhang, Tao Jiang, Yong-Zhi Wang
Summary: The study confirmed the role of METTL3 in IDH-wildtype gliomas, promoting malignant progression by enhancing the stability of MALAT1.
Article
Biochemistry & Molecular Biology
Dariusz Zakrzewicz, Joachim Geyer
Summary: Hepatitis B virus infections are a major public health concern worldwide. A recently discovered high-affinity hepatic host receptor has become a key focus of research for potential therapies. This article presents a newly generated three-dimensional model of the receptor and discusses its role in the entry of HBV into hepatocytes.
Review
Oncology
Yue-Tao Tan, Jin-Fei Lin, Ting Li, Jia-Jun Li, Rui-Hua Xu, Huai-Qiang Ju
Summary: The interactions between alterations in cancer-associated energy metabolism and lncRNA-mediated posttranslational modifications play a crucial role in tumor initiation and progression. A better understanding of lncRNA-mediated cancer metabolic reprogramming can help identify new strategies for cancer diagnosis and therapy.
CANCER COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Michael D. Barnhart, Yi Yang, Erick E. Nakagaki-Silva, Thomas H. Hammond, Mariavittoria Pizzinga, Clare Gooding, Katherine Stott, Christopher W. J. Smith
Summary: The study reveals that phosphorylation of RBPMS protein can rapidly modulate the splicing program in smooth muscle cells. This phosphorylation leads to a decrease in RBPMS activity, affecting RNA binding and splicing regulation. ERK2 is identified as the kinase responsible for phosphorylation at Thr113 and Thr118.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Immunology
Pu Zhang, Zijian Liu, Decai Wang, Yunxue Li, Yifei Xing, Yajun Xiao
Summary: This study comprehensively described alterations of 26 RNA modification writer genes in BLCA and constructed a ten-gene signature with predictive and prognostic value for BLCA patients' overall survival. The writer-related gene signature was also associated with EMT-related pathways and immune characteristics, indicating its potential role in predicting patients who will benefit from immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
William A. Cantara, Chathuri Pathirage, Joshua Hatterschide, Erik D. Olson, Karin Musier-Forsyth
Summary: The phosphorylation of LysRS in HIV-1 infection plays a crucial role in the release and targeting of tRNA primer. Phosphorylated LysRS binds to gRNA and promotes the release of the tRNA primer, leading to a new working model for the interaction between LysRS and gRNA.
Article
Multidisciplinary Sciences
Neil R. Lloyd, Deborah S. Wuttke
Summary: Cyp33 is an essential human cyclophilin prolyl isomerase that plays various roles in splicing and chromatin remodeling, particularly through direct interaction with the MLL1 complex. Understanding how RNA binding drives the action of Cyp33 reveals its regulatory mechanism and interaction with the MLL1 complex.
Review
Cardiac & Cardiovascular Systems
Kun Zhao, Yukang Mao, Yansong Li, Chuanxi Yang, Kai Wang, Jing Zhang
Summary: This review summarizes the emerging advances in epigenetic regulation in pathological myocardial remodeling and provides an overview of the potential mechanisms involved in this regulation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Malgorzata M. Duszczyk, Harry Wischnewski, Tamara Kazeeva, Rajika Arora, Fionna E. Loughlin, Christine von Schroetter, Ugo Pradere, Jonathan Hall, Constance Ciaudo, Frederic H. -T. Allain
Summary: DND1 is an RNA-binding protein essential for germline development, mediating its function through a non-canonical AU-rich RNA recognition mode by the tandem RRMs, while the dsRBD plays a role in recruiting effector complexes for target regulation. The authors report an unusual mode of AU-rich RNA recognition by the RRMs of DND1 in a 27.5 kDa NMR structure and provide additional insight on DND1 function from cell-based experiments.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Neelam Shah, Simone A. Beckham, Jacqueline A. Wilce, Matthew C. J. Wilce
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Jianrong Sang, Ketav Kulkarni, Gabrielle M. Watson, Xiuquan Ma, David J. Craik, Sonia T. Henriques, Aaron G. Poth, Aurelie H. Benfield, Jacqueline A. Wilce
Article
Biochemistry & Molecular Biology
Fionna E. Loughlin, Jacqueline A. Wilce
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Gabrielle M. Watson, Jacqueline A. Wilce
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Irene Garcia-Palmero, Neelam Shah, Naveid A. Ali, Roger J. Daly, Jacqueline A. Wilce, Antonio Villalobo
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2020)
Article
Endocrinology & Metabolism
Monica P. Goney, Matthew C. J. Wilce, Jacqueline A. Wilce, William A. Stocker, Georgia M. Goodchild, Karen L. Chan, Craig A. Harrison, Kelly L. Walton
Article
Biochemistry & Molecular Biology
Simone A. Beckham, Mehdi Y. Matak, Matthew J. Belousoff, Hariprasad Venugopal, Neelam Shah, Naveen Vankadari, Hans Elmlund, Joseph H. C. Nguyen, Bert L. Semler, Matthew C. J. Wilce, Jacqueline A. Wilce
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biochemical Research Methods
Joseph Polidano, Naveen Vankadari, John T. Price, Jacqueline A. Wilce
PROTEIN EXPRESSION AND PURIFICATION
(2020)
Article
Biochemistry & Molecular Biology
Fionna E. Loughlin, Danella L. West, Menachem J. Gunzburg, Saboora Waris, Simon A. Crawford, Matthew C. J. Wilce, Jacqueline A. Wilce
Summary: TIA-1 is an RNA-binding protein that can sequester target RNA into stress granules under stress conditions, with its self-association and liquid-liquid phase separation depend on multiple nucleic acid binding sites. The presence of tandem binding sites is crucial in enhancing phase separation of TIA-1, which is finely tuned by the protein:binding site stoichiometry rather than nucleic acid length. Native tandem TIA-1 binding sites within the 3' UTR of p53 mRNA efficiently enhance phase separation of TIA-1 and may act as potent nucleation sites for stress granule assembly.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Natasha P. Sturre, Rhys N. Colson, Neelam Shah, Gabrielle M. Watson, Xue Yang, Matthew C. J. Wilce, John T. Price, Jacqueline A. Wilce
Summary: The development of peptide inhibitors against intracellular targets requires achieving a balance between high affinity and specificity for the target and cellular permeability. This study demonstrates that incorporating specific amino acids in a bicyclic peptide framework can enhance the binding affinity for a specific protein domain. Interestingly, the inclusion of tryptophan in the bicyclic peptide showed potent inhibition of breast cancer cell migration.
Article
Microbiology
Tomalika R. Ullah, Katherine R. Balka, Rebecca L. Ambrose, Genevieve Pepin, Matthew C. J. Wilce, Jacqueline A. Wilce, Belinda J. Thomas, Dominic De Nardo, Bryan R. G. Williams, Michael P. Gantier
Summary: Cytoplasmic detection of DNA is an essential component of antiviral responses. Genistein, a compound found naturally in soy-based foods, inhibits cGAS-STING antiviral signaling, weakening the antiviral activity.
Article
Biochemistry & Molecular Biology
Danella L. West, Fionna E. Loughlin, Francisco Rivero-Rodriguez, Naveen Vankadari, Alejandro Velazquez-Cruz, Laura Corrales-Guerrero, Irene Diaz-Moreno, Jacqueline A. Wilce
Summary: Stress granules are important non-membrane bound RNA-protein granules for cell survival during stress. The TIA-1 protein plays a key role in stress granule formation through liquid-liquid phase separation, but it is also prone to irreversible fibrillar aggregation. This study reveals that stress granule co-factors Zn2+ and RGG-rich regions from FUS, along with nucleic acids, induce liquid-liquid phase separation of TIA-1 and inhibit fibril formation. These co-factors promote TIA-1 multimerization independently of the prion-like domain, contributing to the dynamic nature of stress granules and cellular protection.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ketav Kulkarni, Jianrong Sang, Xiuquan Ma, Jacqueline A. Wilce
Article
Biochemistry & Molecular Biology
Ketav Kulkarni, Gabrielle M. Watson, Jianrong Sang, Jacqueline A. Wilce
Article
Immunology
Naveen Vankadari, Jacqueline A. Wilce
EMERGING MICROBES & INFECTIONS
(2020)
Article
Cell Biology
Ke Mi, Lizhong Zeng, Yang Chen, Jingya Ning, Siyuan Zhang, Peilin Zhao, Shuanying Yang
Summary: In this study, the researchers explored the role of DHX38 in NSCLC and its underlying molecular mechanism. They found that DHX38 was overexpressed in NSCLC and patients with high DHX38 expression had poor prognosis. DHX38 promoted cell proliferation, migration, and invasion in NSCLC and activated the MAPK pathway. The researchers also identified G3BP1 as a target protein that interacted with DHX38 and showed that DHX38 regulated the expression of G3BP1. Silencing G3BP1 reversed the effects of DHX38 overexpression on tumor cell proliferation, migration, and invasion and inhibited the MAPK pathway activation.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Tiina A. Jokela, Mark A. Dane, Rebecca L. Smith, Kaylyn L. Devlin, Sundus Shalabi, Jennifer C. Lopez, Masaru Miyano, Martha R. Stampfer, James E. Korkola, Joe W. Gray, Laura M. Heiser, Mark A. Labarge
Summary: Microenvironment signals have a significant impact on cell fate and tissue homeostasis. Understanding how different microenvironment factors regulate cellular phenotype has been challenging. In this study, a high-throughput microenvironment microarray was used to identify factors that support the proliferation and maintenance of primary human mammary luminal epithelial cells. Multiple factors that modulate luminal cell number were identified and their effects were confirmed using RNA sequencing and cell-based functional studies. Hepatocyte growth factor (HGF) was found to be robust to individual variation and played a role in expanding luminal cells. Our approach demonstrates the power of high-dimensional cell-based approaches in dissecting microenvironmental signals.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chao He, Yongfeng Ding, Yan Yang, Gang Che, Fei Teng, Haohao Wang, Jing Zhang, Donghui Zhou, Yanyan Chen, Zhan Zhou, Haiyong Wang, Lisong Teng
Summary: This study categorized gastric cancer patients into three stemness subtypes, each demonstrating distinct prognoses, components of tumor microenvironment (TME) infiltration, and varying sensitivity or resistance to treatment. A stemness risk model was constructed to predict treatment response and prognosis.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haile Zhao, Lijuan Feng, Rui Cheng, Man Wu, Xiaozhou Bai, Lifei Fan, Yaping Liu
Summary: miR-29c-3p is overexpressed in benign and malignant ovarian carcinoma and is associated with poor prognosis. Its overexpression modulates tumorigenesis in ovarian cancer cells, including epithelial-mesenchymal transition, proliferation, migration, and invasion, through the regulation of DNMT3A, TET1, and HBP1. miR-29c-3p may serve as a potential biomarker for clinical diagnosis or co-diagnosis of ovarian carcinoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haiyan Zhao, Fangfang Bi, Mengyuan Li, Yuhan Diao, Chen Zhang
Summary: This study confirmed the tumor suppressor effect of RNF180 on ovarian cancer, elucidated the mechanism of the molecule network related to RNF180 and IPO4 in ovarian cancer, and identified a new therapeutic target for ovarian cancer.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chu Chen, Guanhua Xu, Jiajia Chen, Chunshuai Wu, Jinlong Zhang, Jiawei Jiang, Hongxiang Hong, Zhiming Cui
Summary: This study investigated the role of transcription factor FoxO1 in facet joint osteoarthritis (FJOA) and found that FoxO1 deletion led to severe osteoarthritic changes. Transcriptome sequencing and bioinformatics analysis identified differentially expressed genes (DEGs) and potential key contributors to FJOA. Additionally, over-expression of certain genes and inhibition of others were shown to counteract the impairments caused by FoxO1 deletion in chondrocyte migration and extracellular matrix synthesis. These findings help unravel the molecular mechanisms underlying FJOA and open up promising therapeutic avenues for its treatment.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Wen Deng, Ru Chen, Situ Xiong, Jianqiang Nie, Hailang Yang, Ming Jiang, Bing Hu, Xiaoqiang Liu, Bin Fu
Summary: This study demonstrates that circFSCN1 is upregulated in bladder cancer and associated with cancer-specific survival. CircFSCN1 promotes tumor progression and epithelial-mesenchymal transition in bladder cancer through enhancing MDM2-mediated silencing of p53 by sponging miR-145-5p.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Jun Wu, Weibin Hu, Wenhui Yang, Yihao Long, Kaizhao Chen, Fugui Li, Xiaodong Ma, Xun Li
Summary: Cholesterol biosynthesis and metabolism play critical roles in tumor development and microenvironmental conditions. Squalene Epoxidase (SQLE), the second rate-limiting enzyme in cholesterol synthesis, is found to be uniquely expressed in various cancers, and its expression level is closely associated with tumor mutation burden and microsatellite instability. SQLE expression is negatively correlated with immune cell infiltration. Inhibition of SQLE alters the immune response in the tumor microenvironment. Furthermore, protein metabolism and translation are identified as main binding factors with SQLE.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhihong Zhang, Mingyue Li, Yi Tai, Yue Xing, Hongxiang Zuo, Xuejun Jin, Juan Ma
Summary: ZNF70 plays an important role in colitis-associated colorectal cancer (CAC) by regulating macrophages IL-1 beta secretion to promote HCT116 proliferation. It may serve as a promising target for treating CAC.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zenghong Wu, Gangping Li, Weijun Wang, Kun Zhang, Mengke Fan, Yu Jin, Rong Lin
Summary: This study comprehensively explored the role of immune checkpoints and tumor microenvironment in gastric cancer patients based on genomic data. It constructed an ICIs signature and ICI score to evaluate patient prognosis and heterogeneity.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Yantong Wan, Jieshu Zhou, Panpan Zhang, Xuemei Lin, Hao Li
Summary: This study found that Rac1 plays a role in astrocyte activation and attenuates chronic inflammatory pain by blocking the phosphorylation of NLRP3 inflammasome and NF-kappa B.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhen Wang, Diankun She, Lei Liu, Xianming Hua, Hao Zhu, Lingfeng Yu, Han Wang, Yan Zhu, Gentao Fan, Yicun Wang, Meng Xu, Guangxin Zhou
Summary: Circular RNAs (circRNAs) are non-coding RNAs that play a role in the regulation of various cancers, including osteosarcoma (OS). This study identified circSATB2 as a highly expressed circRNA in OS tissues and cell lines, and demonstrated its involvement in promoting OS proliferation and migration. Mechanistically, circSATB2 was found to regulate the progression of OS by sponging miR-661 and FUS to regulate ZNFX1 mRNA. These findings suggest that circSATB2 could serve as a prognostic marker and therapeutic target for osteosarcoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Kenichi Ogata, Masafumi Moriyama, Tatsuya Kawado, Hiroki Yoshioka, Aiko Yano, Mayu Matsumura-Kawashima, Seiji Nakamura, Shintaro Kawano
Summary: This study found that extracellular vesicles released by induced pluripotent stem cells can reduce inflammatory cell infiltration, increase saliva volume, and decrease the production of antibodies associated with Sjogren's syndrome in a mouse model. The let-7 family in these vesicles may suppress the expression of TLR4 and NF-kappa B, which leads to the inhibition of pro-inflammatory cytokine production through the MAPK pathway.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Mikayla R. Erdelsky, Sarah A. Groves, Charmi Shah, Samantha B. Delios, M. Bibiana Umana, Donald H. Maurice
Summary: Recent evidence suggests that cAMP signaling within the primary cilium plays a crucial role in promoting adipogenic differentiation of 3T3-L1 preadipocytes. In this study, the researchers identified the specific cAMP phosphodiesterases expressed by these cells and found that inhibition of PDE4 promotes FFAR4-mediated adipogenesis. This work could potentially lead to the discovery of more targeted therapeutic approaches for controlling adipogenesis and differentiation of other stem cells.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chun-Hui Liu, Jun-Jie Zhang, Qian-Jin Zhang, Yang Dong, Zhen-Duo Shi, Si-Hao Hong, Hou-Guang He, Wei Wu, Cong-Hui Han, Lin Hao
Summary: Bladder cancer, the most common malignant tumor in the urinary system, is associated with significantly up-regulated expression of P3H4, which is regulated by METTL3 and plays a crucial role in the proliferation, metastasis, and EMT progression of bladder cancer. Targeting this METTL3-P3H4 pathway may serve as a potential therapeutic strategy for bladder cancer.
CELLULAR SIGNALLING
(2024)