Review
Cell Biology
Eugenia V. Gurevich, Vsevolod V. Gurevich
Summary: Many neurotransmitter receptors are part of the G protein-coupled receptor (GPCR) superfamily and undergo two-step homologous desensitization. This process involves phosphorylation, binding of arrestin proteins, and initiation of different signaling pathways through arrestin.
Review
Cell Biology
Jeffrey L. Benovic
Summary: Agonist activation of G protein-coupled receptors leads to sequential interaction with G proteins, GRKs, and arrestins. GRKs play a central role in mediating the switch from G protein to arrestin interaction and controlling processes like receptor desensitization. Early studies on GRK identification and cloning laid the foundation for understanding the structure and function of these enzymes.
Article
Biochemistry & Molecular Biology
Marta Sanchez-Soto, Noelia M. Boldizsar, Kayla A. Schardien, Nora S. Madaras, Blair K. A. Willette, Laura R. Inbody, Christopher Dasaro, Amy E. Moritz, Julia Drube, Raphael S. Haider, R. Benjamin Free, Carsten Hoffman, David R. Sibley
Summary: The recruitment and activation of beta-arrestins to the D2 dopamine receptor (D2R) are not completely dependent on GPCR kinase (GRK)-mediated receptor phosphorylation, highlighting the importance of the GRK subfamily in D2R-beta-arrestin interactions.
Review
Cell Biology
Edda S. F. Matthees, Raphael S. Haider, Carsten Hoffmann, Julia Drube
Summary: This review discusses the regulatory mechanisms of G protein-coupled receptors (GPCRs) by GPCR kinases (GRKs) and beta-arrestins, highlighting the importance of direct GPCR-GRK interactions and tissue-specific expression of GRKs and beta-arrestins in influencing GPCR phosphorylation patterns. The analysis of expression data for GRKs and beta-arrestins in different tissues provides insights into the pathophysiological dysregulation of the GPCR/GRK/beta-arrestin system, indicating the potential key role of tissue-specific perspectives in understanding the individual impact of different GRK isoforms on GPCR regulation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Preethi C. Karnam, Sergey A. Vishnivetskiy, Vsevolod V. Gurevich
Summary: Arrestins are a small family of proteins that bind G protein-coupled receptors (GPCRs) with high affinity to active phosphorylated GPCRs. They must have two sensors, which detect receptor-attached phosphates and the active receptor conformation independently, enabling transition into a high-affinity receptor-binding state. This transition involves a global conformational rearrangement that stabilizes the complex by bringing additional elements of the arrestin molecule in contact with a GPCR.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Michael Ippolito, Jeffrey L. Benovic
Summary: β-adrenergic receptors consist of three subtypes and play important roles in regulating physiological processes. Developing biased agonists could potentially enhance treatment efficacy for diseases.
CELLULAR SIGNALLING
(2021)
Article
Pharmacology & Pharmacy
Jamie J. Manning, Gabriel Rawcliffe, David B. Finlay, Michelle Glass
Summary: This study investigated the impact of phosphorylation modifications in a specific sequence of the CB1 receptor C-terminus on the translocation of Arrestin-2 and Arrestin-3. The results showed that these modifications partially contributed to the translocation of Arrestin, but complete inhibition was only achieved when all phosphorylation sites were mutated. Additionally, the dissociation of G proteins was also impaired proportionally to the extent of Arrestin translocation.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Riko Tatsumi, Saki Aihara, Seiya Matsune, Junken Aoki, Asuka Inoue, Takao Shimizu, Motonao Nakamura
Summary: This study reveals the role of phosphorylation in signal transduction of G protein-coupled receptor BLT1. The results show that different concentrations of LTB4 can induce phosphorylation of BLT1 on Ser(310) and Thr(308), thus regulating its binding with beta-arrestin and altering signal transduction.
Review
Cell Biology
Melissa S. Maginnis
Summary: Viruses exploit GPCR-mediated signaling pathways to invade host cells and reprogram cellular processes for viral replication. Beta-arrestins and GRKs, as crucial cellular factors, play a major role in viral entry and signaling pathway reprogramming. Various viruses, including polyomaviruses, flaviviruses, influenza virus, and SARS-CoV-2, activate or rely on GPCR-mediated pathways, suggesting the conservation of host-cell invasion mechanisms. Therefore, targeting GPCR-mediated pathways holds promise for the development of broad antiviral therapies.
CELLULAR SIGNALLING
(2023)
Article
Biochemistry & Molecular Biology
Cierra A. Birch, Helen Wedegaertner, Lennis B. Orduna-Castillo, Monica L. Gonzalez Ramirez, Huaping Qin, Joann Trejo
Summary: This study reveals the mechanisms by which different cytoprotective responses mediated through specific G protein-coupled receptor co-receptors and G protein receptor kinases (GRKs) in endothelial cells.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Vinay Kumar Sharma, Xuyu Yang, Soo-Kyung Kim, Amirhossein Mafi, Daniel Saiz-Sanchez, Patricia Villanueva-Anguita, Lan Xiao, Asuka Inoue, William A. Goddard, Y. Peng Loh
Summary: Interaction between NF-alpha 1/CPE and 5-HTR1E activates the beta-arrestin/ERK/CREB/BCL2 pathway to protect neurons from oxidative stress and neuroexcitotoxicity, preventing cognitive dysfunction.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Neurosciences
Chia-Ling Hsieh, Yun Yao, Vsevolod V. Gurevich, Jeannie Chen
Summary: Arr1 plays a key role in facilitating the dephosphorylation of rhodopsin in vivo. Mice lacking Arr1 show delayed dephosphorylation of rhodopsin, which cannot be explained by cell stress or downregulation of protein phosphatase 2A.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Multidisciplinary Sciences
Shane C. Wright, Viktoriya Lukasheva, Christian Le Gouill, Hiroyuki Kobayashi, Billy Breton, Samuel Mailhot-Larouche, Elodie Blondel-Tepaz, Nichelle Antunes Vieira, Claudio Costa-Neto, Madeleine Heroux, Nevin A. Lambert, Lucas Tabajara Parreiras-e-Silva, Michel Bouvier
Summary: This study uses bioluminescence resonance energy transfer (BRET) to investigate the translocation and activity of Gq in endosomes, revealing different mechanisms underlying Gq activity at the plasma membrane and endosomes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Ya Zhuo, Valeria L. Robleto, Adriano Marchese
Summary: beta-arrestins are versatile adaptor proteins that regulate various aspects of G protein-coupled receptor signaling. By using APEX-based proximity labeling, potential novel beta-arrestin interacting partners were discovered. Our study demonstrates that beta arr1-APEX can interact with known interacting proteins and label known beta arr1-interacting partners upon agonist stimulation. This study highlights the value of beta arr1-APEX-based proximity labeling in identifying novel players involved in GPCR signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Benjamin Barsi-Rhyne, Aashish Manglik, Mark von Zastrow
Summary: Beta-arrestins are important regulators of cellular signaling, involving in the desensitization and endocytosis of GPCRs. Two different modes of endocytic activity have been discovered, one dependent on the C-terminus and the other dependent on the C-lobe of beta-arrestins. These modes are triggered in a receptor-specific manner and have opposite effects on the signaling output of receptors.
Article
Biochemistry & Molecular Biology
Layara Akemi Abiko, Marco Rogowski, Antoine Gautier, Gebhard Schertler, Stephan Grzesiek
Summary: The study presents strategies for E. coli expression of GPCRs and developed an optimized protocol with quantification of losses in receptor material during solubilization and purification steps, resulting in final yields of 0.2-0.3 mg per liter; High-quality NMR studies were successfully conducted using E. coli-expressed mutants, with similar results to mutants expressed in insect cells.
JOURNAL OF BIOMOLECULAR NMR
(2021)
Article
Chemistry, Medicinal
Rachel A. Rowlands, Qiuyan Chen, Renee A. Bouley, Larisa Avramova, John J. G. Tesmer, Andrew D. White
Summary: The ability of GRK2 and GRK5 to regulate GPCR desensitization makes them attractive targets for heart failure and cancer treatment. Previous research showed potential for selective inhibition of GRK5 over GRK2 using a specific residue, but the inhibitors were not very potent. A new study successfully adapted an indolinone scaffold with covalent warheads, creating compounds with high selectivity for GRK5 and low nanomolar potency.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
M. Claire Cato, Yu-Chen Yen, Charnelle J. Francis, Kaely E. Elkins, Afzaal Shareef, Rachel Sterne-Marr, John J. G. Tesmer
Summary: This paper discusses three distinct models for how GRKs recognize activated GPCRs, addresses limitations in the approaches used to generate them, and experimentally tests a hypothetical GPCR interaction site in GRK2 suggested by the two newest models.
Article
Cell Biology
Valerie Panneels, Ana Diaz, Cornelia Imsand, Manuel Guizar-Sicairos, Elisabeth Mueller, Anne Greet Bittermann, Takashi Ishikawa, Andreas Menzel, Andres Kaech, Mirko Holler, Christian Grimm, Gebhard Schertler
Summary: Ptychographic hard X-ray computed tomography (PXCT) is a recent method that allows imaging with quantitative electron-density contrast, providing isotropic 3D information of cellular and subcellular structures. The potential of PXCT for analyzing disease processes, such as neurodegeneration, at sub-200 nm resolution is illustrated through comparison with tomograms of degenerative retina. PXCT is non-destructive, suitable for correlative imaging, and powerful for tissue volumes analysis.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Na Wu, Agnieszka M. Olechwier, Cyrill Brunner, Patricia C. Edwards, Ching-Ju Tsai, Christopher G. Tate, Gebhard F. X. Schertler, Gisbert Schneider, Xavier Deupi, Renato Zenobi, Pikyee Ma
Summary: Developed high-sensitivity, high-throughput MALDI-MS method to study the interaction between GPCRs and signaling partners, focusing on GPCR-G protein complex formation. Studied over 70 ligand-GPCR-partner protein combinations to determine selectivity and binding affinities, demonstrating the potential for broad use in screening GPCR-targeting drugs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Diane C. A. Barret, Gebhard F. X. Schertler, U. Benjamin Kaupp, Jacopo Marino
Summary: The cryo-EM structure of the bovine rod CNG channel isolated from retina reveals an additional gate within the ion conduction pathway contributed by the CNGB1 subunit, shedding light onto the structural basis and answering long-standing questions regarding the function of CNG channels in visual and olfactory neurons.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Article
Rheumatology
Victor Z. Sun, Terry L. Melim, Soumya Mitra, Jamie E. Erickson, Shaughn H. Bryant, Avery Farnham, Susan Westmoreland, Heather Knight, Liang Zhang, Wendy Ritacco, Kristoff Homan, Lorenzo Benatuil, Annette J. Schwartz Sterman, Andrew D. Goodearl
Summary: This study assessed the ability of monoclonal antibodies specific for FnEDA to target diseased tissues in mouse collagen-induced arthritis models. The targeting was found to be specific and focused on inflamed synovium. The antibodies could accumulate in diseased tissue at relevant concentrations and sustain targeting for up to 14 days. FnEDA was shown to support targeting of multiple doses of the antibodies given 5 days apart.
ADVANCES IN RHEUMATOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Liang Zhang, Yuzhen Wang, Kristoff T. Homan, Stephanie M. Gaudette, Andrew J. McCluskey, Ying Chan, Joanne Murphy, Mary Abdalla, Christine M. Nelson, Victor Z. Sun, Jamie E. Erickson, Heather L. Knight, Anca Clabbers, Annette J. Schwartz Sterman, Soumya Mitra
Summary: This study successfully visualized the alternatively spliced D domain of tenascin C expressed in the colon using near infrared-labeled targeted molecular imaging agents in a murine colitis model. The imaging data suggest that this imaging technique has potential applications in delivery-based therapies for inflammatory bowel diseases.
MOLECULAR IMAGING AND BIOLOGY
(2023)
Article
Chemistry, Physical
Cecilia M. Casadei, Ahmad Hosseinizadeh, Gebhard F. X. Schertler, Abbas Ourmazd, Robin Santra
Summary: Time-resolved serial femtosecond crystallography (TR-SFX) allows for the study of protein dynamics with atomic resolution on sub-picosecond timescales. In this work, the authors propose a novel approach called low-pass spectral analysis (LPSA) to improve the analysis of TR-SFX data. LPSA projects the data onto a subspace defined by trigonometric functions, attenuating high-frequency features and facilitating the retrieval of underlying dynamics. The authors demonstrate the effectiveness of LPSA in reconstructing dynamics and compare it to other existing data analysis techniques.
STRUCTURAL DYNAMICS-US
(2022)
Article
Multidisciplinary Sciences
Yu-Chen Yen, Christopher T. Schafer, Martin Gustaysson, Stefanie A. Eberle, Pawel K. Dominik, Dawid Deneka, Penglie Zhang, Thomas J. Schall, Anthony A. Kossiakoff, John J. G. Tesmer, Tracy M. Handel
Summary: This study presents cryo-EM structures of ACKR3 in complex with CXCL12, a more potent CXCL12 variant, and a small-molecule agonist. The binding poses of these chemokines are different from those established for CXCR4 and observed in other receptor-chemokine complexes. These structures, together with functional studies, provide insights into the ligand-binding promiscuity of ACKR3, its inability to couple to G proteins, and its bias towards beta-arrestin, laying the foundation for understanding the physiological interplay of ACKR3 with other GPCRs.
Article
Multidisciplinary Sciences
Diane C. A. Barret, Dina Schuster, Matthew J. Rodrigues, Alexander Leitner, Paola Picotti, Gebhard F. X. Schertler, U. Benjamin Kaupp, Volodymyr M. Korkhov, Jacopo Marino
Summary: Calmodulin (CaM) regulates ion channels to control calcium entry into cells, and mutations affecting this interaction are linked to fatal diseases. The structural basis of CaM regulation is not well understood. In retinal photoreceptors, CaM binds to the CNGB subunit of CNG channels and modulates the channel's sensitivity to changes in ambient light. This study provides the structural characterization of CaM regulation of a CNG channel using cryo-electron microscopy and proteomics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemical Research Methods
Matthew J. Rodrigues, Cecilia M. Casadei, Tobias Weinert, Valerie Panneels, Gebhard F. X. Schertler
Summary: Rhodopsin is a light-sensitive G-protein-coupled receptor that plays a crucial role in vertebrate vision. By using serial femtosecond crystallography, the structure of rhodopsin was successfully solved at room temperature. However, a lattice-translocation defect (LTD) was discovered in the crystals, which required correction to accurately interpret the data. This correction was essential in order to confidently model the unilluminated state of the receptor and analyze the light-activated data.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2023)
Article
Chemistry, Physical
Cecilia M. Casadei, Ahmad Hosseinizadeh, Spencer Bliven, Tobias Weinert, Jorg Standfuss, Russell Fung, Gebhard F. X. Schertler, Robin Santra
Summary: Low-pass spectral analysis (LPSA) is an effective algorithm for retrieving dynamics in model data affected by incompleteness and weighting errors. In this study, LPSA is applied to experimental time-resolved crystallography data and the parametric sensitivity is analyzed. The presence of high-frequency contamination in dynamical modes is investigated using synthetic data with various uncertainties and errors. A method is proposed to handle missing observations and improved dynamics retrieval is achieved.
STRUCTURAL DYNAMICS-US
(2023)
Article
Biochemistry & Molecular Biology
Jessica J. Waninger, Tyler S. Beyett, Varun V. Gadkari, Ronald F. Siebenaler, Carson Kenum, Sunita Shankar, Brandon T. Ruotolo, Arul M. Chinnaiyan, John J. G. Tesmer
Summary: Oncogenic mutations in the KRAS gene result in a constitutively active form, making direct targeting of KRAS challenging. A study identified AGO2 as a protein that interacts with RAS-driven oncogenesis. While purified AGO2 was shown to co-immunoprecipitate with KRAS, the complex between FL AGO2 and KRAS could not be isolated, indicating further research is needed to understand the interaction of KRAS with purified AGO2 forms.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Article
Cell Biology
Ke Mi, Lizhong Zeng, Yang Chen, Jingya Ning, Siyuan Zhang, Peilin Zhao, Shuanying Yang
Summary: In this study, the researchers explored the role of DHX38 in NSCLC and its underlying molecular mechanism. They found that DHX38 was overexpressed in NSCLC and patients with high DHX38 expression had poor prognosis. DHX38 promoted cell proliferation, migration, and invasion in NSCLC and activated the MAPK pathway. The researchers also identified G3BP1 as a target protein that interacted with DHX38 and showed that DHX38 regulated the expression of G3BP1. Silencing G3BP1 reversed the effects of DHX38 overexpression on tumor cell proliferation, migration, and invasion and inhibited the MAPK pathway activation.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Tiina A. Jokela, Mark A. Dane, Rebecca L. Smith, Kaylyn L. Devlin, Sundus Shalabi, Jennifer C. Lopez, Masaru Miyano, Martha R. Stampfer, James E. Korkola, Joe W. Gray, Laura M. Heiser, Mark A. Labarge
Summary: Microenvironment signals have a significant impact on cell fate and tissue homeostasis. Understanding how different microenvironment factors regulate cellular phenotype has been challenging. In this study, a high-throughput microenvironment microarray was used to identify factors that support the proliferation and maintenance of primary human mammary luminal epithelial cells. Multiple factors that modulate luminal cell number were identified and their effects were confirmed using RNA sequencing and cell-based functional studies. Hepatocyte growth factor (HGF) was found to be robust to individual variation and played a role in expanding luminal cells. Our approach demonstrates the power of high-dimensional cell-based approaches in dissecting microenvironmental signals.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chao He, Yongfeng Ding, Yan Yang, Gang Che, Fei Teng, Haohao Wang, Jing Zhang, Donghui Zhou, Yanyan Chen, Zhan Zhou, Haiyong Wang, Lisong Teng
Summary: This study categorized gastric cancer patients into three stemness subtypes, each demonstrating distinct prognoses, components of tumor microenvironment (TME) infiltration, and varying sensitivity or resistance to treatment. A stemness risk model was constructed to predict treatment response and prognosis.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haile Zhao, Lijuan Feng, Rui Cheng, Man Wu, Xiaozhou Bai, Lifei Fan, Yaping Liu
Summary: miR-29c-3p is overexpressed in benign and malignant ovarian carcinoma and is associated with poor prognosis. Its overexpression modulates tumorigenesis in ovarian cancer cells, including epithelial-mesenchymal transition, proliferation, migration, and invasion, through the regulation of DNMT3A, TET1, and HBP1. miR-29c-3p may serve as a potential biomarker for clinical diagnosis or co-diagnosis of ovarian carcinoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Haiyan Zhao, Fangfang Bi, Mengyuan Li, Yuhan Diao, Chen Zhang
Summary: This study confirmed the tumor suppressor effect of RNF180 on ovarian cancer, elucidated the mechanism of the molecule network related to RNF180 and IPO4 in ovarian cancer, and identified a new therapeutic target for ovarian cancer.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chu Chen, Guanhua Xu, Jiajia Chen, Chunshuai Wu, Jinlong Zhang, Jiawei Jiang, Hongxiang Hong, Zhiming Cui
Summary: This study investigated the role of transcription factor FoxO1 in facet joint osteoarthritis (FJOA) and found that FoxO1 deletion led to severe osteoarthritic changes. Transcriptome sequencing and bioinformatics analysis identified differentially expressed genes (DEGs) and potential key contributors to FJOA. Additionally, over-expression of certain genes and inhibition of others were shown to counteract the impairments caused by FoxO1 deletion in chondrocyte migration and extracellular matrix synthesis. These findings help unravel the molecular mechanisms underlying FJOA and open up promising therapeutic avenues for its treatment.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Wen Deng, Ru Chen, Situ Xiong, Jianqiang Nie, Hailang Yang, Ming Jiang, Bing Hu, Xiaoqiang Liu, Bin Fu
Summary: This study demonstrates that circFSCN1 is upregulated in bladder cancer and associated with cancer-specific survival. CircFSCN1 promotes tumor progression and epithelial-mesenchymal transition in bladder cancer through enhancing MDM2-mediated silencing of p53 by sponging miR-145-5p.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Jun Wu, Weibin Hu, Wenhui Yang, Yihao Long, Kaizhao Chen, Fugui Li, Xiaodong Ma, Xun Li
Summary: Cholesterol biosynthesis and metabolism play critical roles in tumor development and microenvironmental conditions. Squalene Epoxidase (SQLE), the second rate-limiting enzyme in cholesterol synthesis, is found to be uniquely expressed in various cancers, and its expression level is closely associated with tumor mutation burden and microsatellite instability. SQLE expression is negatively correlated with immune cell infiltration. Inhibition of SQLE alters the immune response in the tumor microenvironment. Furthermore, protein metabolism and translation are identified as main binding factors with SQLE.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhihong Zhang, Mingyue Li, Yi Tai, Yue Xing, Hongxiang Zuo, Xuejun Jin, Juan Ma
Summary: ZNF70 plays an important role in colitis-associated colorectal cancer (CAC) by regulating macrophages IL-1 beta secretion to promote HCT116 proliferation. It may serve as a promising target for treating CAC.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zenghong Wu, Gangping Li, Weijun Wang, Kun Zhang, Mengke Fan, Yu Jin, Rong Lin
Summary: This study comprehensively explored the role of immune checkpoints and tumor microenvironment in gastric cancer patients based on genomic data. It constructed an ICIs signature and ICI score to evaluate patient prognosis and heterogeneity.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Yantong Wan, Jieshu Zhou, Panpan Zhang, Xuemei Lin, Hao Li
Summary: This study found that Rac1 plays a role in astrocyte activation and attenuates chronic inflammatory pain by blocking the phosphorylation of NLRP3 inflammasome and NF-kappa B.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Zhen Wang, Diankun She, Lei Liu, Xianming Hua, Hao Zhu, Lingfeng Yu, Han Wang, Yan Zhu, Gentao Fan, Yicun Wang, Meng Xu, Guangxin Zhou
Summary: Circular RNAs (circRNAs) are non-coding RNAs that play a role in the regulation of various cancers, including osteosarcoma (OS). This study identified circSATB2 as a highly expressed circRNA in OS tissues and cell lines, and demonstrated its involvement in promoting OS proliferation and migration. Mechanistically, circSATB2 was found to regulate the progression of OS by sponging miR-661 and FUS to regulate ZNFX1 mRNA. These findings suggest that circSATB2 could serve as a prognostic marker and therapeutic target for osteosarcoma.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Kenichi Ogata, Masafumi Moriyama, Tatsuya Kawado, Hiroki Yoshioka, Aiko Yano, Mayu Matsumura-Kawashima, Seiji Nakamura, Shintaro Kawano
Summary: This study found that extracellular vesicles released by induced pluripotent stem cells can reduce inflammatory cell infiltration, increase saliva volume, and decrease the production of antibodies associated with Sjogren's syndrome in a mouse model. The let-7 family in these vesicles may suppress the expression of TLR4 and NF-kappa B, which leads to the inhibition of pro-inflammatory cytokine production through the MAPK pathway.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Mikayla R. Erdelsky, Sarah A. Groves, Charmi Shah, Samantha B. Delios, M. Bibiana Umana, Donald H. Maurice
Summary: Recent evidence suggests that cAMP signaling within the primary cilium plays a crucial role in promoting adipogenic differentiation of 3T3-L1 preadipocytes. In this study, the researchers identified the specific cAMP phosphodiesterases expressed by these cells and found that inhibition of PDE4 promotes FFAR4-mediated adipogenesis. This work could potentially lead to the discovery of more targeted therapeutic approaches for controlling adipogenesis and differentiation of other stem cells.
CELLULAR SIGNALLING
(2024)
Article
Cell Biology
Chun-Hui Liu, Jun-Jie Zhang, Qian-Jin Zhang, Yang Dong, Zhen-Duo Shi, Si-Hao Hong, Hou-Guang He, Wei Wu, Cong-Hui Han, Lin Hao
Summary: Bladder cancer, the most common malignant tumor in the urinary system, is associated with significantly up-regulated expression of P3H4, which is regulated by METTL3 and plays a crucial role in the proliferation, metastasis, and EMT progression of bladder cancer. Targeting this METTL3-P3H4 pathway may serve as a potential therapeutic strategy for bladder cancer.
CELLULAR SIGNALLING
(2024)