Article
Chemistry, Multidisciplinary
Yogesh A. Bhadange, Virendra Kumar Saharan
Summary: This study compares the extraction of D-galacturonic acid from basil seeds using different extraction techniques. The results show that ultrasonic extraction has a higher yield and shorter extraction time compared to stirred reactor and soxhlet extraction. The process effectiveness of ultrasonic extraction is significantly better than the other two methods. The study also investigates the kinetics of the extraction process and characterizes the extracted components.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Biotechnology & Applied Microbiology
Andreea Perpelea, Andy Wiranata Wijaya, Luis C. Martins, Dorthe Rippert, Mathias Klein, Angel Angelov, Kaisa Peltonen, Attila Teleki, Wolfgang Liebl, Peter Richard, Johan M. Thevelein, Ralf Takors, Isabel Sa-Correia, Elke Nevoigt
Summary: This study successfully demonstrated the co-fermentation of D-GalUA and glycerol by introducing the fungal D-GalUA pathway into yeast strain S. cerevisiae, resulting in the production of ethanol at a high rate in synthetic minimal medium. The fast and complete consumption of D-GalUA in crude sugar beet pulp hydrolysate was also confirmed, indicating potential for industrial application.
METABOLIC ENGINEERING
(2022)
Article
Green & Sustainable Science & Technology
Chatuphon Siamphan, Jantima Arnthong, Sudarat Tharad, Fei Zhang, Jie Yang, Thanaporn Laothanachareon, Santi Chuetor, Verawat Champreda, Xin-Qing Zhao, Surisa Suwannarangsee
Summary: The study successfully expressed the exopolygalacturonase (exoPG) enzyme from Aspergillus aculeatus in the industrial strain Trichoderma reesei RUT-C30, and achieved the goal of increasing the production of D-galacturonic acid under conditions of 60 degrees Celsius, pH 6.0, and 48 hours.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Agriculture, Multidisciplinary
Alexandra Fatouros, Ulrike Einhorn-Stoll, Hanna Kastner, Stephan Drusch, Lothar W. Kroh
Summary: The study aims to clarify the reasons for high browning potential in sugar acids by measuring mutarotation velocity and CO2 elimination during heat treatment. Results show that the difference in mutarotation velocity between D-galacturonic acid and D-galactose, as well as the release of CO2, may play a role in the chemical mechanisms underlying the high browning potential.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ting Wang, Meiyun Jiang, Shuo Tang, Chenhuan Lai, Caoxing Huang, Yimin Fan, Qiang Yong
Summary: A novel biorefining strategy was established to prepare di-GalA and tri-GalA from commercial pectin, with hydrothermal pretreatment and enzymatic hydrolysis enhancing the yield and selectivity of oligogalacturonic acids.
PROCESS BIOCHEMISTRY
(2021)
Article
Chemistry, Physical
Sashikumar Ramamirtham, Martin A. K. Williams, Davoud Zare, Mike Weeks, Catherine P. Whitby
Summary: Complexation of high-methylesterified pectins with beta-lactoglobulin at pH 4 can strengthen protein films at dodecane/water interfaces, doubling the steady state shear elastic moduli. The charge density and pattern of the polysaccharide primarily determine the lag times of film formation. Adsorbed beta-lactoglobulin molecules cross-link in the aqueous phase, strengthening the resulting interfacial layer.
Article
Microbiology
Dorthe Rippert, Federica Linguardo, Andreea Perpelea, Mathias Klein, Elke Nevoigt
Summary: The study identified a specific enzyme in baker's yeast, Gcy1, responsible for the conversion of d-GalUA to l-GalA, shedding light on the process of valorising d-GalUA in Saccharomyces cerevisiae.
Article
Chemistry, Physical
Alessandro Boverio, Hugo L. van Beek, Simone Savino, Adeline Ranoux, Wouter J. J. Huijgen, Harry W. C. Raaijmakers, Marco W. Fraaije, Nikola Loncar
Summary: This study reports the discovery of a new flavin-containing carbohydrate oxidase URAO(Cs3) from Citrus sinensis, which showed high thermostability and selectivity for uronic acids. The enzyme was highly effective in selectively oxidizing D-galacturonic acid, while leaving other carbohydrates untouched. These findings suggest that plant proteomes can serve as an interesting source for new biocatalysts.
Article
Biotechnology & Applied Microbiology
Anu Tamminen, Rosaliina Turunen, Dorothee Barth, Virve Vidgren, Marilyn G. Wiebe
Summary: Mucic acid, which has potential applications in various industries, can be produced from D-galacturonic acid found in pectin through microbial conversion. The study found that a newly generated transformant of a marine Trichoderma species showed high production of mucic acid in glucose-limited fed-batch culture. Yeast extract was not necessary for high production, but increased the initial production rate.
Article
Agriculture, Multidisciplinary
Pengpu Wang, Guoyu Sun, Pei Lu, Yuchen Zhu, Xiaosong Hu, Fang Chen
Summary: This study focuses on the role of galacturonic acid in the formation of acrylamide. The results show that the addition of galacturonic acid can accelerate the formation of acrylamide, and the formation of acrylamide is closely related to pH value and temperature. Moreover, some intermediates were identified, contributing to the formation of acrylamide in fruit products.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biotechnology & Applied Microbiology
J. Wagner, D. Schafer, N. von den Eichen, C. Haimerl, S. Harth, M. Oreb, J. P. Benz, D. Weuster-Botz
Summary: This study successfully demonstrated the conversion of pectin in sugar beet press pulp to L-galactonate using engineered Saccharomyces cerevisiae. Through simple batch processes, the potential for sustainable production of high-value chemicals was shown.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Carol N. Flores-Fernandez, Max Cardenas-Fernandez, Gary J. Lye, John M. Ward
Summary: This study investigates the application of thermophilic pectinases in the bioconversion of pectin-rich biomass into bio-based chemical building blocks. The results show that these pectinases exhibit optimum activity and stability at high temperatures, and the synergistic action between pectin methylesterases and exo-polygalacturonases increases the efficiency of pectin monomerization. This study provides new insights into the application of thermophilic pectinases.
ENZYME AND MICROBIAL TECHNOLOGY
(2022)
Article
Polymer Science
Khodidash Baraiya, Virendra Kumar Yadav, Nisha Choudhary, Daoud Ali, Daya Raiyani, Vibhakar A. Chowdhary, Sheena Alooparampil, Rohan V. Pandya, Dipak Kumar Sahoo, Ashish Patel, Jigna G. Tank
Summary: This research compares the physicochemical parameters of pectin isolated from the peels of seven citrus fruits and explores its potential use as a thickening agent, gelling agent, and food ingredient in food industries. The hydrophobic nature of pectin purified from Citrus limon, Citrus limetta, Citrus sudachi, and Citrus sinensis, and the hydrophilic nature of pectin purified from Citrus maxima, Citrus jambhiri, and Citrus hystrix, suggest their different applications in the preparation of hydrogels, nanofibers, food packaging material, polysoaps, drug delivery agents, and microparticulate materials, and gelling and thickening agents respectively.
Review
Clinical Neurology
Pascal Jorratt, Cyril Hoschl, Saak Ovsepian
Summary: Schizophrenia is a chronic neuropsychiatric brain disorder with pathobiology related to hypofunction of the NMDA receptor. Endogenous NMDA receptor antagonists play a crucial role in the onset and progression of the disease, highlighting the need to restore NMDA receptor function in future studies for therapeutic benefits.
ALZHEIMERS & DEMENTIA
(2021)
Review
Agricultural Engineering
Deokyeol Jeong, Heeyoung Park, Byeong-Kwan Jang, YeBin Ju, Min Hye Shin, Eun Joong Oh, Eun Jung Lee, Soo Rin Kim
Summary: Citrus peel waste (CPW) emerges as a promising and sustainable option for biorefinery due to its industrial potential of biological valorization into fuels and chemicals, despite the specific technical difficulties in fermenting CPW, such as high pectin content. Recent advances in the metabolic engineering of yeast and other microbial strains for fermenting CPW-derived sugars to produce value-added products, like ethanol and mucic acid, offer potential for industrially viable CPW-based biorefinery with improved fermentation efficiency and diversified product profiles.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rodrigo Papa-Gobbi, Cecilia I. Muglia, Andres Rocca, Renata Curciarello, Alicia M. Sambuelli, Martin Yantorno, Gustavo Correa, Luciano G. Morosi, Antonio Di Sabatino, Paolo Biancheri, Thomas T. MacDonald, Marta A. Toscano, Karina V. Marino, Gabriel A. Rabinovich, Guillermo H. Docena
Summary: The study found that transient regulation of O-glycan profile during inflammation modulates Gal-1 binding and LP T-cell survival in IBD patients.
Article
Multidisciplinary Sciences
Alejandro J. Cagnoni, Maria Laura Giribaldi, Ada G. Blidner, Anabela M. Cutine, Sabrina G. Gatto, Rosa M. Morales, Mariana Salatino, Martin C. Abba, Diego O. Croci, Karina V. Marino, Gabriel A. Rabinovich
Summary: Colorectal cancer is a common malignancy with limited response to immunotherapy, prompting the need for new biomarkers and therapeutic targets. Gal-1, as an endogenous glycan-binding protein, has been found to influence the activity of CD8(+) regulatory T cells in CRC, suggesting a potential immunotherapeutic approach for this disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Nadia Bannoud, Tomas Dalotto-Moreno, Lucia Kindgard, Pablo A. Garcia, Ada G. Blidner, Karina V. Marino, Gabriel A. Rabinovich, Diego O. Croci
Summary: This study highlights the reciprocal regulation between hypoxia, angiogenesis, and immunosuppression in tumor progression and anti-tumor therapies. The findings suggest a rational basis for optimizing synergistic combinations of antiangiogenic and immunotherapeutic strategies to enhance treatment efficacy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cecilia Porciuncula-Gonzalez, Alejandro J. Cagnoni, Carolina Fontana, Karina Marino, Patricia Saenz-Mendez, Cecilia Giacomini, Gabriela Irazoqui
Summary: This study evaluated the binding affinity of Gal-1 for various galactosides, showing weaker affinity for beta-(1->6) galactosides compared to beta-(1->4) and beta-(1->3) galactosides. Docking experiments revealed key interactions between Gal-1 and specific amino acid residues of evaluated galactosides, contributing to the understanding of galectin-glycan affinity.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Luciano G. Morosi, Anabela M. Cutine, Alejandro J. Cagnoni, Montana N. Manselle-Cocco, Diego O. Croci, Joaquin P. Merlo, Rosa M. Morales, Maria May, Juan M. Perez-Saez, Maria R. Girotti, Santiago P. Mendez-Huergo, Betiana Pucci, Anibal H. Gil, Sergio P. Huernos, Guillermo H. Docena, Alicia M. Sambuelli, Marta A. Toscano, Gabriel A. Rabinovich, Karina Marino
Summary: The study reveals a glycosylation-dependent on-off circuit controlled by Galectin-1 (Gal1) and its glycosylated ligands which target activated CD8(+) T cells and shape cytokine profile to maintain intestinal homeostasis. In patients with inflammatory bowel disease, increased Gal1 is associated with dysregulated expression of glycosyltransferases C2GNT1 and ST6GAL1. This provides insights into the role of Gal1 and its glycosylated ligands in recalibrating T cell immunity to preserve intestinal homeostasis.
Article
Oncology
Valeria da Costa, Sandra J. van Vliet, Paula Carasi, Sofia Frigerio, Pablo A. Garcia, Diego O. Croci, Maria Florencia Festari, Monique Costa, Mercedes Landeira, Santiago A. Rodriguez-Zraquia, Alejandro J. Cagnoni, Anabela M. Cutine, Gabriel A. Rabinovich, Eduardo Osinaga, Karina Marino, Teresa Freire
Summary: Tn antigen, originating from tumor cells, interacts with the MGL receptor to promote immunosuppression and angiogenesis, ultimately favoring tumor progression.
Article
Materials Science, Multidisciplinary
A. Bordoni, M. Lombardo, A. Wolosiuk
Summary: In this study, the use of microwave radiation as a postsynthesis treatment for expanding the pore size of non-calcined SiO2-based mesoporous materials was analyzed. Compared to traditional hydrothermal treatments, microwave irradiation significantly reduces the processing time and is compatible with various surfactant pore templates. This approach allows for easy postprocessing of non-calcined templated siliceous materials and customization of their final pore dimensions.
MATERIALS CHEMISTRY AND PHYSICS
(2021)
Review
Chemistry, Organic
Carla Marino, Andrea V. Bordoni
Summary: Deoxy sugars play a crucial role in various biological processes and can be used for studying enzymes and enzyme analogs to discover new therapeutic agents. Deoxygenation of sugars can be utilized to improve the bioactivity of natural products and explore new therapeutic drugs.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Cell Biology
M. Constanza Baroni Pietto, Paola R. Lev, Ana C. Glembotsky, Cecilia P. Marin Oyarzun, Graciela Gomez, Victoria Collado, Cecilia Pisoni, Ramiro A. Gomez, Matias Grodzielski, Jacqueline Gonzalez, Karina Marino, Paula G. Heller, Nora P. Goette, Rosana F. Marta
Summary: The study found that systemic lupus erythematosus (SLE) patients with thrombocytopenia have abnormalities in platelet clearance and production, mainly due to apoptosis and desialylation leading to low platelet count. These abnormalities are more frequently observed in patients with thrombocytopenia and active disease.
Article
Biochemistry & Molecular Biology
Brenda Lucila Jofre, Ricardo Javier Elicabe, Juan Eduardo Silva, Juan Manuel Perez Saez, Maria Daniela Paez, Eduardo Callegari, Karina Valeria Marino, Maria Silvia Di Genaro, Gabriel Adrian Rabinovich, Roberto Carlos Davicino
Summary: Galactin-1 (Gal1) binds to Yops in a carbohydrate-dependent manner, protecting them from trypsin digestion. Gal1 and YopP cooperatively inhibit nitric oxide (NO) production, without influencing other inflammatory mediators. In Yersinia enterocolitica infection, Gal1 and YopP play a coordinated role in modulating macrophage function.
Article
Chemistry, Physical
Santiago Poklepovich-Caride, Victor Oestreicher, M. Mercedes Zalduendo, Andrea Bordoni, Galo J. A. A. Soler-Illia, Paula C. Angelome
Summary: This study presents a simplified and accelerated method for the synthesis of gold nanoparticles (AuNPs) and demonstrates the influence of pH control and combination of multiple reductants on AuNP characteristics. The method allows for the production of AuNPs with different shapes and sizes, offering potential applications in various fields.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Review
Materials Science, Biomaterials
M. Veronica Rivas, Maria J. Arenas Muneton, Andrea V. Bordoni, M. Veronica Lombardo, Carla C. Spagnuolo, Alejandro Wolosiuk
Summary: The carboxylic chemical group has various applications, such as being a part of amino acids, a ligand for transition metals, and a stabilizer. Silica serves as an inert platform for anchoring multiple chemical entities, allowing for the functioning of an integrated chemical system. Different methods have been developed for the surface chemical modification of silica, using precursors like organoalkoxysilanes. This review analyzes the importance of COOH groups in materials science and presents new approaches for obtaining COOH-based organoalkoxide precursors.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Review
Biotechnology & Applied Microbiology
Karina V. Marino, Alejandro J. Cagnoni, Diego O. Croci, Gabriel A. Rabinovich
Summary: This article discusses the therapeutic relevance of galectins to cancer and fibrosis, and reviews the various strategies to target galectins and their progress in clinical trials.
NATURE REVIEWS DRUG DISCOVERY
(2023)
Article
Chemistry, Organic
Enrique A. Del Vigo, Carlos A. Stortz, Carla Marino
Summary: This paper focuses on the regioselectivity in glycosidation reactions of d-allose glycopyranosyl acceptors. The results show that axial O-3 is the preferred site for glycosylation of alpha-anomers, while equatorial O-4 is the preferred site for beta-anomers. The experimental results are in good agreement with molecular modeling.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
