Article
Oncology
Margherita Piccolella, Riccardo Cristofani, Barbara Tedesco, Marta Chierichetti, Veronica Ferrari, Elena Casarotto, Marta Cozzi, Valeria Crippa, Paola Rusmini, Mariarita Galbiati, Angelo Poletti, Elio Messi
Summary: This study investigated the impact of retinoic acid (RA) on the gene expression of small heat shock protein B8 (HSPB8) and BCL-2 associated athanogene 3 (BAG3) in breast cancer cells. The results showed that RA can decrease the expression of both HSPB8 and BAG3 genes, and also affect the mitotic spindle organization in the cells. The effects of RA on HSPB8 levels are suggested to be mediated by miR-574-5p, highlighting a potential therapeutic strategy for counteracting HSPB8 in breast cancer progression.
FRONTIERS IN ONCOLOGY
(2021)
Article
Mycology
Faezeh Malekinejad, Johanna Fink-Gremmels, Hassan Malekinejad
Summary: This study investigated the effects of Zearalenone (ZEN) and its metabolites on the formation of carcinogenic estrogen catechols in MCF-7 cells. The results showed that mycoestrogens significantly inhibited the activity of cytochrome P450 1A1 (CYP 1A1) and enhanced the activity of CYP 1B1. Additionally, mycoestrogens upregulated the expression of aryl-hydrocarbon receptor (AhR) and increased the production of CYP1B1-mediated estrogen catechol metabolites.
MYCOTOXIN RESEARCH
(2023)
Article
Cell Biology
Xiangchan Hou, Le Tang, Xiayu Li, Fang Xiong, Yongzhen Mo, Xianjie Jiang, Xiangying Deng, Miao Peng, Pan Wu, Mengyao Zhao, Jiawei Ouyang, Lei Shi, Yi He, Qijia Yan, Shanshan Zhang, Zhaojian Gong, Guiyuan Li, Zhaoyang Zeng, Fuyan Wang, Can Guo, Wei Xiong
Summary: Breast cancer is the most common malignant tumor in women, with KCNK6 being significantly overexpressed in breast cancer cells and promoting their proliferation, invasion, and migration abilities. Targeting KCNK6 may offer a novel therapeutic strategy for breast cancer treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Environmental Sciences
Vijayakumar Gunasekaran, Rathinam Yuvakkumar, Ravi Ganesan, Surya Cholayil Palapetta, Harichandran Gurusamy
Summary: In this study, NBA and ABA were synthesized and evaluated for their cell multiplication inhibition activity against MCF-7 and HeLa cancer cell lines. The results showed that both NBA and ABA exhibited antiproliferative action against these cancer cell lines.
ENVIRONMENTAL RESEARCH
(2023)
Article
Cell Biology
Giuseppe Garroni, Francesca Balzano, Sara Cruciani, Renzo Pala, Donatella Coradduzza, Emanuela Azara, Emanuela Bellu, Maria Laura Cossu, Giorgio C. Ginesu, Ciriaco Carru, Carlo Ventura, Margherita Maioli
Summary: The exhausted medium from MCF-7 influences the behavior of hADSCs by increasing the expression of stemness-related genes, cell proliferation, and autophagy. Polyamines detectable in the MCF-7 exhausted medium may be related to the higher proliferation capability observed in hADSCs, suggesting a direct crosstalk between these molecules and the changes in stem cell potency.
Article
Multidisciplinary Sciences
Ola E. E. Abdel-Sattar, Rasha Mosa Allam, Ahmed M. M. Al-Abd, Bharathi Avula, Kumar Katragunta, Ikhlas A. A. Khan, Ahmed M. M. El-Desoky, Shanaz O. O. Mohamed, Ali El-Halawany, Essam Abdel-Sattar, Meselhy R. R. Meselhy
Summary: The genus Phyllanthus has potential in the treatment of various diseases, and its extracts showed cytotoxicity against breast cancer cells. The methylene chloride fraction exhibited the strongest cytotoxic activity among all tested fractions.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, Research & Experimental
Konrad A. Szychowski, Bartosz Skora, Tadeusz Pomianek
Summary: The study evaluated the impact of VGVAPG and VVGPGA on human breast adenocarcinoma and human lung carcinoma cells, showing an increase in KI67 protein expression and influence on antioxidant enzyme expression.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Biochemistry & Molecular Biology
Giovanna Azzarito, Michele Visentin, Brigitte Leeners, Raghvendra K. Dubey
Summary: Vascular and lymphatic vessels play a crucial role in breast cancer growth and metastasis. The study found that the secretome of estrogen receptor-positive breast cancer cells can stimulate vascular angiogenesis and inhibit lymphatic growth, which has significant pathological and physiological implications for breast cancer progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
John Alfon P. Francisco, Monissa C. Paderes
Summary: This study describes the design and synthesis of a series of BCE-ring-truncated Deguelin analogues, which exhibit significant anticancer activity and favorable in silico predictions for physicochemical properties, druglikeness, and toxicity. These findings provide valuable insights for the further development of novel anticancer agents.
Article
Cell Biology
Giovanna Azzarito, Margit Henry, Tamara Rotshteyn, Brigitte Leeners, Raghvendra K. Dubey
Summary: miR193a-3p is a tumor suppressor that inhibits the growth of VECs and alters the secretome of MCF-7 breast cancer cells. It also inhibits the growth of LECs and affects ERK1/2 and Akt phosphorylation, which are important for cancer development and metastasis. MiR193a-3p can modify the tumor microenvironment and has potential as a therapeutic molecule for breast cancer.
Article
Integrative & Complementary Medicine
Ashok Mari, Gopikrishnan Mani, Sirpu Natesh Nagabhishek, Gopalakrishnan Balaraman, Nirmala Subramanian, Fathima Bushra Mirza, Jagan Sundaram, Devaki Thiruvengadam
Summary: Carvacrol significantly inhibited the proliferation of breast cancer MCF-7 cells and induced apoptosis by suppressing the PI3/AKT signaling pathway.
CHINESE JOURNAL OF INTEGRATIVE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jun Li, Song-He Wang, Yang-Ting Liu, Qin Zhang, Guang-Zhou Zhou
Summary: EF-24 exhibited antiproliferative and antimigratory activities on MCF-7 cells. It inhibited autophagic flux, induced apoptosis, and showed crosstalk between autophagy and apoptosis in breast cancer cells. These findings provide a solid foundation for further investigation of EF-24's potential anticancer activity on MCF-7 cells.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Judit Doczi, Noemi Karnok, David Bui, Victoria Azarov, Gergely Pallag, Sara Nazarian, Bence Czumbel, Thomas N. N. Seyfried, Christos Chinopoulos
Summary: This study investigates the effects of severe hypoxia, site-specific inhibition of respiratory chain components, and uncouplers on 2D-cultured HepG2 and MCF-7 tumor cells. The results show that there is no correlation between oxidative phosphorylation activity and viability in these cell lines, indicating that the connection between oxidative phosphorylation and cancer cell survival is dependent on cell type and conditions.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Wenna Shao, Xinzhao Wang, Zhaoyun Liu, Xiang Song, Fukai Wang, Xiaoyu Liu, Zhiyong Yu
Summary: Breast cancer is the most prevalent cancer worldwide, and resistance to the first-line chemotherapeutic agent Adriamycin is a common cause of treatment failure. The study focuses on the role of oxidative stress factors ROS and NRF2 in breast cancer and aims to develop therapeutic drugs that can enhance chemotherapy sensitivity. The combination of ADR and CYT showed potent anti-breast cancer activity by inhibiting cell activity, inducing apoptosis, and blocking the cell cycle in breast cancer cells through ROS generation and the P62/NRF2/HO-1 signaling pathway. The effectiveness of ADR + CYT in suppressing tumor development was also observed in breast cancer-bearing mice.
SCIENTIFIC REPORTS
(2023)
Article
Pharmacology & Pharmacy
Alexander Chota, Blassan P. George, Heidi Abrahamse
Summary: This study investigated the effects of Dicoma anomala methanol root extract on breast cancer cells and found that it decreased cell viability and increased apoptosis. When combined with photodynamic therapy, the effects were more significant.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alexander Negoda, Elizabeth A. Cowley, Yassine El Hiani, Paul Linsdell
CELLULAR AND MOLECULAR LIFE SCIENCES
(2018)
Article
Biochemistry & Molecular Biology
Man-Song Li, Elizabeth A. Cowley, Yassine El Hiani, Paul Linsdell
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Paul Linsdell
Article
Neurosciences
Daniel Saucier, Gabriel Wajnberg, Jeremy Roy, Annie-Pier Beauregard, Simi Chacko, Nicolas Crapoulet, Sebastien Fournier, Anirban Ghosh, Stephen M. Lewis, Alier Marrero, Colleen O'Connell, Rodney J. Ouellette, Pier Jr Morin
Review
Medical Laboratory Technology
Jeremy Roy, Daniel Saucier, Colleen O'Connell, Pier Morin
CLINICA CHIMICA ACTA
(2019)
Article
Biochemistry & Molecular Biology
Paul Linsdell, Alexander Negoda, Elizabeth A. Cowley, Yassine El Hiani
CELL BIOCHEMISTRY AND BIOPHYSICS
(2020)
Article
Biochemistry & Molecular Biology
Paul Linsdell
Summary: The study found that the chloride conductance of the CFTR channel is mainly determined by the ability of anions to bind in the pore already occupied by chloride ions, rather than by anion:anion interactions within the pore. Although anion binding is necessary for high conductance, mutations that strengthen anion binding have little effect on conductance, indicating that the wild-type CFTR pore is already close to saturated with chloride ions.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Multidisciplinary Sciences
Jeremy W. Roy, Catherine A. Taylor, Annie P. Beauregard, Surendar R. Dhadi, D. Craig Ayre, Sheena Fry, Simi Chacko, Gabriel Wajnberg, Andrew P. Joy, Ngoc-Nu Mai-Thi, Nicolas Crapoulet, David A. Barnett, Anirban Ghosh, Stephen M. Lewis, Rodney J. Ouellette
Summary: Extracellular vesicles (EVs) are rich material for biomarker analysis, and an optimized method using Vn96 peptide has been developed for efficient isolation of EVs and cfDNA from plasma samples, allowing for downstream analysis of DNA, RNA, and protein. This multiparametric extraction protocol enhances the sensitivity and specificity of liquid biopsy tests utilizing EVs and cfDNA biomarkers for disease detection and monitoring.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Paul Linsdell
Summary: The CFTR chloride channel has weak selectivity for divalent anions, with stronger binding observed internally compared to externally. Mutant channels with additional positive charges in the pore exhibit significantly enhanced blocking by divalent anions.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Paul Linsdell, Christina L. Irving, Elizabeth A. Cowley, Yassine El Hiani
Summary: Positively charged amino acid side-chains are important for anion binding and permeation through the CFTR chloride channel. The lysine residue K95 and arginine residue R134 within the pore play crucial roles in anion binding and conductance properties. Despite the importance of specific positive charges, it is the overall number of charges rather than their exact locations that control pore function.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Cell Biology
Awanit Kumar, Surendar Reddy Dhadi, Ngoc-Nu Mai, Catherine Taylor, Jeremy W. Roy, David A. Barnett, Stephen M. Lewis, Anirban Ghosh, Rodney J. Ouellette
Summary: Chitosan has been demonstrated as a potential method for isolating small extracellular vesicles efficiently from various biofluids. This method offers advantages such as high efficiency, biocompatibility, and low toxicity.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biochemistry & Molecular Biology
Paul Linsdell
Summary: Cystic fibrosis is caused by mutations in CFTR gene. The F337 amino acid within the selectivity filter plays a crucial role in the channel's conductivity and selectivity. Anion selectivity depends on side-chain size and hydrophobicity. Unique interactions between permeating Cl- ions and the native phenylalanine side-chain are important for channel's conductance.
JOURNAL OF MEMBRANE BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alexander Negoda, Mairin S. Hogan, Elizabeth A. Cowley, Paul Linsdell
CELLULAR AND MOLECULAR LIFE SCIENCES
(2019)