Article
Oncology
Garima Dixit, Jesus Gonzalez-Bosquet, Joseph Skurski, Eric J. J. Devor, Erin B. B. Dickerson, Warren B. B. Nothnick, Priya D. D. Issuree, Kimberly K. K. Leslie, Thorsten Maretzky
Summary: Mutations in the FGFR2 gene have been linked to advanced and recurrent endometrial cancer. This study reveals that FGFR2 mutations enhance the activation of ADAM17 and the subsequent transactivation of EGFR in response to FGF7. Furthermore, FGFR2 mutants also activate the Notch signaling pathway in an ADAM17-dependent manner. These findings highlight the importance of ADAM17 in the pathogenesis of endometrial cancer and provide a rationale for targeting ADAM17 protease activity in FGFR2-driven cancers.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Oncology
Lijuan Zhang, Meng Tian, Jiamao Lin, Jianbo Zhang, Haiyong Wang, Zhenxiang Li
Summary: The interaction between ER beta 1 and ER beta 5 in lung adenocarcinoma affects non-genomic signaling and resistance to EGFR TKIs. Cytoplasmic ER beta 1 may contribute to EGFR TKI resistance, and PC9/ER beta 1/5 cells show higher resistance to gefitinib.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Mengkai Yang, Tao Zhang, Yangfeng Zhang, Xiaojun Ma, Jing Han, Ke Zeng, Yafei Jiang, Zongyi Wang, Zhuoying Wang, Jing Xu, Yingqi Hua, Zhengdong Cai, Wei Sun
Summary: The study revealed that MYLK4 promotes the growth and metastasis of osteosarcoma by activating the EGFR signaling pathway. Additionally, the combination of MYLK4 and EGFR inhibitors showed synergistic effects on the growth and metastasis of OS in vitro and in vivo.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biology
Yongjian Huang, Jana Ognjenovic, Deepti Karandur, Kate Miller, Alan Merk, Sriram Subramaniam, John Kuriyan
Summary: Research has shown that the EGFR receptor can adopt different conformations of the extracellular module when binding to different ligands, with implications for intracellular signaling pathways.
Article
Biochemistry & Molecular Biology
Garima Dixit, Willow Schanz, Benjamin A. Pappas, Thorsten Maretzky
Summary: Metalloproteases ADAM10 and ADAM17 have been identified as differentially regulated FGFR membrane sheddases, playing roles in modulating FGFR signaling during cell migration. Ectodomain shedding can impact the function of ligand-induced FGFR signaling in cell movement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Brendan Sullivan, Taylor Light, Vinh Vu, Adrian Kapustka, Kalina Hristova, Deborah Leckband
Summary: This study reveals a force-transduction mechanism that connects mechanical perturbations of E-cadherin to the activation of EGFR, a key regulator of cell proliferation. The results demonstrate that E-cadherin and EGFR form complexes at the plasma membrane, which are disrupted by the application of force or the presence of EGF. The findings provide insights into the initial steps of E-cadherin-mediated force transduction and the activation of growth regulatory signaling cascades.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Pharmacology & Pharmacy
Jazlyn P. Borges, Katrina Mekhail, Gregory D. Fairn, Costin N. Antonescu, Benjamin E. Steinberg
Summary: Chronic pain is a major public health issue that is often resistant to conventional analgesics. Recent studies have implicated the epidermal growth factor receptor (EGFR) signaling pathway in chronic pain, suggesting potential therapeutic targets for this devastating condition.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Soubiya Mohammed Rizwan Ansari, Farah Saleh Hijazi, Serhiy Souchelnytskyi
Summary: DNA-PKcs has been identified as a regulator of carcinogenesis, with increased expression correlating with metastatic cancers. Recent reports have highlighted the crosstalk between DNA-PKcs and ER, PR, and EGFR, revealing a complex network of interactions and providing insights into potential diagnostic and therapeutic strategies for cancer.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Biology
Sandeep K. Singhal, Jung S. Byun, Samson Park, Tingfen Yan, Ryan Yancey, Ambar Caban, Sara Gil Hernandez, Stephen M. Hewitt, Heike Boisvert, Stephanie Hennek, Mark Bobrow, Shakir Uddin Ahmed, Jason White, Clayton Yates, Andrew Aukerman, Rami Vanguri, Rohan Bareja, Romina Lenci, Paula Lucia Farre, Adriana De Siervi, Anna Maria Napoles, Nasreen Vohra, Kevin Gardner
Summary: The study applies digital pathology to analyze the subcellular distribution of the transcription factor Kaiso in breast cancer tumors, revealing novel associations with other biomarkers and providing insights into Kaiso's role in breast cancer progression. Automated image analysis quantifies the distribution of Kaiso, showing correlations with breast cancer subtype, overall survival, and a link with the autophagy marker LC3.
COMMUNICATIONS BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Alina Kurylowicz
Summary: Menopausal decline in estrogen levels leads to changes in adipose tissue distribution and increased obesity prevalence in women. Hormone replacement therapy can partially restore these effects, indicating the significant role of estrogen in adipocyte regulation. Preclinical studies have shown involvement of estrogen in adipose tissue development, metabolism, and inflammation. However, the relationship between estrogen and obesity is bidirectional, as estrogen deficiency leads to fat accumulation and impairs adipocyte function, while obese individuals have altered expression of estrogen receptors and key enzymes involved in their synthesis. This narrative review provides an overview of estrogen's role in adipose tissue development, physiology, and obesity-related dysfunction, as well as discussing potential therapeutic applications in obesity treatment.
Article
Cell Biology
Long Ngo Hoang, Sook-Jeong Lee
Summary: Specific inhibition of casein kinase 1 (CK1) controls 4-hydroxytamoxifen (4-OHT)-mediated endometrial carcinogenesis by blocking the activation and translocation of ER/EGFR and GPER1/Src. EGFR and GPER1 reciprocally regulate each other in endometrial cell carcinogenesis through direct interaction. Inhibition of CK1 may be a potential adjuvant therapy for breast cancer patients taking tamoxifen.
CELLULAR SIGNALLING
(2023)
Article
Reproductive Biology
Sihai Lu, Hui Zhang, Yaju Tang, Rui Xu, Jie Liu, Ru Yao, Juncai Wei, Chan Li, Xiaoe Zhao, Qiang Wei, Baohua Ma
Summary: This study demonstrated that G protein-coupled estrogen receptor (GPER) regulates oocyte maturation in goats by modulating the expression of natriuretic peptide receptor 2 (NPR2) and epidermal growth factor (EGF)-like factors. Activation of the downstream EGF signaling network by GPER leads to a decrease in cyclic guanosine monophosphate (cGMP) synthesis and acceleration of meiotic resumption in goat oocytes.
Review
Cell Biology
Joanne L. Peterson, Brian P. Ceresa
Summary: Maintaining proper structure and function of the cornea is essential for clear vision. The epidermal growth factor receptor (EGFR) plays a crucial role in maintaining and restoring the corneal epithelium. Recent advances in understanding EGFR signaling and trafficking offer new strategies to overcome receptor desensitization and optimize receptor signaling for corneal health.
Article
Orthopedics
Zhihua Lu, Aihua Zhang, Jingcheng Wang, Kuijing Han, Han Gao
Summary: This study aimed to investigate the effect of estrogen on the progression of post-traumatic osteoarthritis (PTOA) in mice and its possible mechanism. The results showed that decreased estrogen can aggravate the weight of female mice, and the degree of cartilage damage in the knee joint on the traumatized side was more severe. Estrogen supplementation can reduce cartilage damage in the knee joint on the traumatized side of female mice, and the damage is less serious than that of male mice. Moreover, as estrogen levels decreased, the severity of cartilage erosion on the traumatized side worsened, and the expression of p-EGFR in the cartilage surface was higher in female mice.
BMC MUSCULOSKELETAL DISORDERS
(2022)
Article
Gastroenterology & Hepatology
Juhoon So, Minwook Kim, Seung-Hoon Lee, Sungjin Ko, Daniel A. Lee, Hyewon Park, Mizuki Azuma, Michael J. Parsons, David Prober, Donghun Shin
Summary: The differentiation of liver progenitor cells (LPCs) into hepatocytes is crucial for reducing inflammation and fibrosis in chronic liver diseases. Inhibition of the epidermal growth factor receptor (EGFR) signaling pathway promotes LPC-to-hepatocyte differentiation and may serve as a proregenerative therapeutic approach for patients with advanced liver disease. This study provides insights into the mechanisms underlying LPC-mediated liver regeneration and suggests the potential of EGFR inhibitors as a treatment option.
Article
Multidisciplinary Sciences
Chengzu Long, Hui Li, Malte Tiburcy, Cristina Rodriguez-Caycedo, Viktoriia Kyrychenko, Huanyu Zhou, Yu Zhang, Yi-Li Min, John M. Shelton, Pradeep P. A. Mammen, Norman Y. Liaw, Wolfram-Hubertus Zimmermann, Rhonda Bassel-Duby, Jay W. Schneider, Eric N. Olson
Article
Multidisciplinary Sciences
Leonela Amoasii, John C. W. Hildyard, Hui Li, Efrain Sanchez-Ortiz, Alex Mireault, Daniel Caballero, Rachel Harron, Thaleia-Rengina Stathopoulou, Claire Massey, John M. Shelton, Rhonda Bassel-Duby, Richard J. Piercy, Eric N. Olson
Article
Multidisciplinary Sciences
Yi-Li Min, Hui Li, Cristina Rodriguez-Caycedo, Alex A. Mireault, Jian Huang, John M. Shelton, John R. McAnally, Leonela Amoasii, Pradeep P. A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Article
Multidisciplinary Sciences
Leonela Amoasii, Hui Li, Yu Zhang, Yi-Li Min, Efrain Sanchez-Ortiz, John M. Shelton, Chengzu Long, Alex A. Mireault, Samadrita Bhattacharyya, John R. McAnally, Rhonda Bassel-Duby, Eric N. Olson
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Yu Zhang, Hui Li, Yi-Li Min, Efrain Sanchez-Ortiz, Jian Huang, Alex A. Mireault, John M. Shelton, Jiwoong Kim, Pradeep P. A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Article
Cell Biology
Miao Cui, Zhaoning Wang, Kenian Chen, Akansha M. Shah, Wei Tan, Lauren Duan, Efrain Sanchez-Ortiz, Hui Li, Lin Xu, Ning Liu, Rhonda Bassel-Duby, Eric N. Olson
DEVELOPMENTAL CELL
(2020)
Article
Biotechnology & Applied Microbiology
Yi-Li Min, Francesco Chemello, Hui Li, Cristina Rodriguez-Caycedo, Efrain Sanchez-Ortiz, Alex A. Mireault, John R. McAnally, John M. Shelton, Yu Zhang, Rhonda Bassel-Duby, Eric N. Olson
Article
Multidisciplinary Sciences
F. Chemello, A. C. Chai, H. Li, C. Rodriguez-Caycedo, E. Sanchez-Ortiz, A. Atmanli, A. A. Mireault, N. Liu, R. Bassel-Duby, E. N. Olson
Summary: This study demonstrated the use of adenine base editor to restore dystrophin expression in cardiomyocytes affected by Duchenne muscular dystrophy mutation, as well as reframing the dystrophin open reading frame. In vivo experiments with mice showed successful gene editing and disease correction, indicating the potential of nucleotide editing for diverse DMD mutations with minimal genome modification.
Article
Medicine, Research & Experimental
Yu Zhang, Takahiko Nishiyama, Hui Li, Jian Huang, Ayhan Atmanli, Efrain Sanchez-Ortiz, Zhaoning Wang, Alex A. Mireault, Pradeep P. A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Summary: A study introduced an efficient gene-editing method using SaCas9 to correct the commonly affected exon 51 in DMD, which showed promising therapeutic effects in correcting DMD in a mouse model through a single AAV9 delivery system.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Medicine, Research & Experimental
Yu Zhang, Hui Li, Takahiko Nishiyama, John R. McAnally, Efrain Sanchez-Ortiz, Jian Huang, Pradeep P. A. Mammen, Rhonda Bassel-Duby, Eric N. Olson
Summary: In this study, a humanized DMD mouse model was created to evaluate the efficacy of CRISPR-Cas9 gene editing for DMD correction. The model successfully restored dystrophin expression and improved pathological features and muscle strength. This model provides a valuable tool for assessing clinically relevant gene editing strategies and advancing the therapeutic translation of CRISPR-Cas9 gene editing for DMD correction.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Multidisciplinary Sciences
Simon Lebek, Francesco Chemello, Xurde M. Caravia, Wei Tan, Hui Li, Kenian Chen, Lin Xu, Ning Liu, Rhonda Bassel-Duby, Eric N. Olson
Summary: CRISPR-Cas9 gene editing is a promising therapy for genomic mutations, but current approaches are limited to specific mutations in a small group of patients. This study introduces a cardioprotective strategy that can potentially benefit a broader range of heart disease patients. By using base editing to eliminate oxidation-sensitive methionine residues in the CaMKII6 gene, the researchers demonstrated protection against ischemia/reperfusion injury in human cardiomyocytes and restored heart function in mice.
Article
Cell Biology
Takahiko Nishiyama, Yu Zhang, Miao Cui, Hui Li, Efrain Sanchez-Ortiz, John R. McAnally, Wei Tan, Jiwoong Kim, Kenian Chen, Lin Xu, Rhonda Bassel-Duby, Eric N. Olson
Summary: Mutations in the RBM20 gene are a common cause of familial dilated cardiomyopathy. The study uses gene editing techniques to correct these mutations and demonstrates that correction can restore normal gene splicing and nuclear localization, while eliminating abnormal ribonucleoprotein granule formation. The effectiveness of this therapy is also validated in a mouse model.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Retraction
Medicine, Research & Experimental
Huijuan Shi, Hui Li, Tiantian Zhen, Yu Dong, Xiaojuan Pei, Xiangliang Zhang
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Biochemistry & Molecular Biology
Tao Long, Yichi Zhang, Linda Donnelly, Hui Li, Yu-Chung Pien, Ning Liu, Eric N. Olson, Xiaochun Li
Summary: The researchers determined the structure of the key protein Myomaker involved in myoblast fusion and found that it adopts a GPCR-like fold. They also identified the dimeric interface and lipid-binding sites as crucial for fusion and proposed a mechanism that may be conserved between different species.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Francesco Chemello, Zhaoning Wang, Hui Li, John R. McAnally, Ning Liu, Rhonda Bassel-Duby, Eric N. Olson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)