Article
Toxicology
Chun-Hong Yu, Shui-Qing Yang, Yu-Jing Zhang, Long Rong, Zong-Chun Yi
Summary: This study investigated the role of GATA factors in hydroquinone (HQ) inhibited erythroid differentiation in K562 cells. The results showed that HQ reduced the occupancy of GATA1 and increased the expression of GATA2, thereby inhibiting erythroid differentiation.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Emi Murayama, Catherine Vivier, Anne Schmidt, Philippe Herbomel
Summary: Mesenchymal stromal cells in hematopoietic niches are derived from the sclerotome section of somites and express Alcam and Pdgfr-alpha markers. These cells are crucial for the development of hematopoiesis, including the formation of the vascular component of the CHT niche and the emergence of HSPCs from the aorta.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Nickolai A. Tchurikov, Elena S. Klushevskaya, Ildar R. Alembekov, Antonina N. Kretova, Vladimir R. Chechetkin, Galina I. Kravatskaya, Yuri V. Kravatsky
Summary: The expression of rDNA gene clusters affects pluripotency, but the mechanisms behind it are unknown. These clusters interact with multiple genes controlling differentiation in both human and Drosophila cells, indicating their potential role in 3D chromosomal structure formation and gene expression regulation. However, it is unclear whether inter-chromosomal rDNA contacts change during differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Seung-Hoon Lee, Na Rae Park, Jung-Eun Kim
Summary: In this study, gene expression changes during megakaryocyte maturation were analyzed using the GeneChip microarray technology, and functional classification and protein interaction analysis of the differentially expressed genes were performed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Wei Zhuo, Meng Sun, Kun Wang, Lu Zhang, Kai Li, Danyang Yi, Mengjie Li, Qiang Sun, Xixi Ma, Wei Liu, Lisong Teng, Chengqi Yi, Tianhua Zhou
Summary: This study reveals the significance of PCIF1 and m(6)Am modification in gastric cancer. Increased expression of PCIF1 and m(6)Am modification are associated with gastric cancer progression and poor prognosis. Silencing PCIF1 inhibits the proliferation and invasion of gastric cancer cells, and suppresses tumor growth and metastasis in a mouse model. PCIF1 modifies TM9SF1 mRNA with m(6)Am, leading to decreased TM9SF1 translation. TM9SF1 reverses the effects of PCIF1 on gastric cancer cell aggressiveness.
Article
Multidisciplinary Sciences
Zhigang Li, Xuemei Fu, Weiru Wu, Zhilong Liu, Zhe Chen, Chengfang Zhou, Yuanyuan Liu, Mei Kuang, Fangfang Sun, Feifei Xiao, Yongxiu Huang, Xiaojun Zhang, Shijun Fan, Xingqin Huang, Guangling Zheng, Jieping Chen, Yu Hou
Summary: Zfp521 is a critical regulator of quiescence and self-renewal of HSC in adult hematopoiesis, mediating its effects partly through controlling Rela expression, especially under regenerative stress conditions.
Article
Microbiology
Yves-Marie Boudehen, Yara Tasrini, John Jairo Aguilera-Correa, Mattheo Alcaraz, Laurent Kremer
Summary: Mycobacterium abscessus is a multi-drug-resistant non-tuberculous mycobacterial species causing tuberculosis-like lung infections. This study developed a method to silence the expression of specific genes in M. abscessus during host infection, demonstrating the essentiality of mmpL3 for bacterial growth.
MICROBIOLOGY SPECTRUM
(2023)
Article
Hematology
Emanuele Gioacchino, Cansu Koyunlar, Joke Zink, Hans de Looper, Madelon de Jong, Tomasz Dobrzycki, Christopher B. Mahony, Remco Hoogenboezem, Dennis Bosch, Paulina M. H. van Strien, Martin E. van Royen, Pim J. French, Eric Bindels, Kirsten J. Gussinklo, Rui Monteiro, Ivo P. Touw, Emma de Pater
Summary: Through studying Gata2b-deficient zebrafish, this research demonstrates the role of GATA2 in hematopoiesis, showing a decrease in myeloid lineage differentiation and a shift towards lymphoid lineage differentiation in the absence of Gata2b.
Article
Developmental Biology
Lexie Prokopuk, Ellen G. Jarred, Rheannon O. Blucher, Eileen A. McLaughlin, Jessica M. Stringer, Patrick S. Western
Summary: This study reveals the crucial role of PRC2 in ovarian development and function. By analyzing a mouse model with reduced H3K27me3, it was found that PRC2 is essential for the survival of female animals and plays a significant role in ovarian development and morphology.
Article
Cell Biology
Yu-Chuen Huang, Chun-Ping Huang, Chin-Ping Lin, Kai-Chien Yang, Yu-Jie Lei, Hao-Pei Wang, Yueh-Hsiung Kuo, Yu-Jen Chen
Summary: Daphnoretin extracted from the stem and roots of Wikstroemia indica has been found to inhibit cell growth and induce megakaryocytic differentiation in chronic myeloid leukemia cells. It shows potential as a differentiation therapy agent for CML, but further investigations are needed to validate its efficacy in vivo and in patients.
Article
Biochemistry & Molecular Biology
Giulia Lombardi, Roberta Valeria Latorre, Alessandro Mosca, Diego Calvanese, Luisa Tomasello, Christian Boni, Manuela Ferracin, Massimo Negrini, Nader Al Dewik, Mohamed Yassin, Mohamed A. Ismail, Bruno Carpentieri, Claudio Sorio, Paola Lecca
Summary: This study analyzed the modulation of the K562 transcriptome in the context of Chronic Myeloid Leukemia (CML) using the tumor suppressor gene PTPRG and the tyrosine kinase inhibitor Imatinib. The analysis identified genes whose expression levels were altered by PTPRG and Imatinib, providing insights into the transcriptomic landscape of CML. Two different methods were used in the analysis, with one focusing on gene set enrichment analysis and the identification of transcription factors, and the other focusing on selecting transcription factors from differentially expressed genes. The study revealed the potential upregulation of RARG and CD36 genes by PTPRG, suggesting a role in cell differentiation. The findings provide new insights into the molecular mechanisms of tumor suppression in the context of CML.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Ben Yue, Ran Cui, Ruizhe Zheng, Weilin Jin, Chenlong Song, Tianshang Bao, Ming Wang, Fengrong Yu, Enhao Zhao
Summary: ALKBH5 is essential for bile acid-induced gastric IM, demethylating ZNF333 mRNA to enhance ZNF333 expression, leading to CDX2 and NF-kappa B activation, and forming a positive feedforward loop. Targeting the ALKBH5/ZNF333/CYLD/CDX2 axis may be a promising therapeutic strategy for patients with bile regurgitation-related IM.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Biology
Jianhua Zhang, Zachery R. Gregorich, Ran Tao, Gina C. Kim, Pratik A. Lalit, Juliana L. Carvalho, Yogananda Markandeya, Deane F. Mosher, Sean P. Palecek, Timothy J. Kamp, Gordana Vunjak-Novakovic
Summary: This study reveals the importance of extracellular matrix (ECM) proteins, specifically fibronectin (FN), in promoting cardiac differentiation of human pluripotent stem cells (hPSCs). The presence of FN in the ECM is essential for effective cardiac differentiation, while other ECM proteins like laminin-111 and laminin-521 inhibit this process. Inhibition of certain integrin receptors and integrin-linked kinase can also impair cardiac differentiation.
Article
Multidisciplinary Sciences
Alexander Mattebo, Taha Sen, Maria Jassinskaja, Kristyna Pimkova, Isabel Prieto Gonzalez-Albo, Abdul Ghani Alattar, Ramprasad Ramakrishnan, Stefan Lang, Marcus Jaras, Jenny Hansson, Shamit Soneji, Sofie Singbrant, Emile van den Akker, Johan Flygare
Summary: YPEL family genes are highly conserved and potentially involved in essential cellular processes. Ypel4, specifically expressed in late terminal erythroid differentiation, plays a role in maintaining normal red cell membrane integrity. Ypel4-null mice exhibit secondary polycythemia and intrinsic defects in red blood cells.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Stefan A. Berghoff, Lena Spieth, Ting Sun, Leon Hosang, Constanze Depp, Andrew O. Sasmita, Martina H. Vasileva, Patricia Scholz, Yu Zhao, Dilja Krueger-Burg, Sven Wichert, Euan R. Brown, Kyriakos Michail, Klaus-Armin Nave, Stefan Bonn, Francesca Odoardi, Moritz Rossner, Till Ischebeck, Julia M. Edgar, Gesine Saher
Summary: Research has shown that in chronic demyelinating diseases, increased neuronal cholesterol synthesis plays a critical role in remyelination, especially when lipid recycling is defective in the affected area. This finding contributes to understanding disease progression and has implications for therapeutic strategies in patients with multiple sclerosis (MS).
Article
Clinical Neurology
Brett J. O'Donnell, Lanping Guo, Samit Ghosh, Faraaz A. Shah, Patrick J. Strollo, Bryan J. McVerry, Mark T. Gladwin, Solomon F. Ofori-Acquah, Gregory J. Kato, Christopher P. O'Donnell
SLEEP AND BREATHING
(2019)
Letter
Hematology
Samit Ghosh, Bethany Flage, Frances Weidert, Solomon F. Ofori-Acquah
BRITISH JOURNAL OF HAEMATOLOGY
(2019)
Article
Developmental Biology
Peter J. Bosch, Leah C. Fuller, Joshua A. Weiner
Article
Hematology
Oluwabukola T. Gbotosho, Samit Ghosh, Maria G. Kapetanaki, Yu Lin, Frances Weidert, Grant C. Bullock, Solomon F. Ofori-Acquah, Gregory J. Kato
BRITISH JOURNAL OF HAEMATOLOGY
(2019)
Review
Neurosciences
Sara C. Taylor, Sarah L. Ferri, Mahip Grewal, Zoe Smernoff, Maja Bucan, Joshua A. Weiner, Ted Abel, Edward S. Brodkin
BIOLOGICAL PSYCHIATRY
(2020)
Article
Hematology
Solomon F. Ofori-Acquah, Rimi Hazra, Oluwaseun O. Orikogbo, Danielle Crosby, Bethany Flage, Ezekiel B. Ackah, Diane Lenhart, Roderick J. Tan, Dario A. Vitturi, Vivian Paintsil, Ellis Owusu-Dabo, Samit Ghosh
Article
Hematology
Oluwabukola T. Gbotosho, Maria G. Kapetanaki, Mark Ross, Samit Ghosh, Frances Weidert, Grant C. Bullock, Simon Watkins, Solomon F. Ofori-Acquah, Gregory J. Kato
EXPERIMENTAL HEMATOLOGY
(2020)
Article
Genetics & Heredity
Andrew M. Garrett, Peter J. Bosch, David M. Steffen, Leah C. Fuller, Charles G. Marcucci, Alexis A. Koch, Preeti Bais, Joshua A. Weiner, Robert W. Burgess
Review
Biochemistry & Molecular Biology
Peter J. Bosch, Stacey L. Peek, Sarit Smolikove, Joshua A. Weiner
CELLULAR AND MOLECULAR LIFE SCIENCES
(2020)
Article
Biology
Srijit Das, Felicia K. Ooi, Johnny Cruz Corchado, Leah C. Fuller, Joshua A. Weiner, Veena Prahlad
Article
Neurosciences
David M. Steffen, Sarah L. Ferri, Charles G. Marcucci, Kelsey L. Blocklinger, Michael J. Molumby, Ted Abel, Joshua A. Weiner
Summary: Cell adhesion molecules play a key role in neural circuit formation, with gamma-protocadherins negatively regulating neuroligins to limit synapse density. Loss of gamma-Pcdhs results in increased inhibitory synapse density and abnormal behavior phenotypes.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Stacey L. Peek, Peter J. Bosch, Ethan Bahl, Brianna J. Iverson, Mrutyunjaya Parida, Preeti Bais, J. Robert Manak, Jacob J. Michaelson, Robert W. Burgess, Joshua A. Weiner
Summary: Akirin2 is critical for maintaining healthy neurons during cortical maturation, with p53 pathways identified as mediators of its functions. Reduction of Trp53 can rescue neurodegeneration caused by Akirin2 loss.
Article
Neurosciences
David M. Steffen, Camille M. Hanes, Kar Men Mah, Paula Valino Ramos, Peter J. Bosch, Dalton C. Hinz, Jason J. Radley, Robert W. Burgess, Andrew M. Garrett, Joshua A. Weiner
Summary: The establishment of a functional cerebral cortex relies on the proper execution of various developmental steps, which includes dendritic and axonal outgrowth, synaptic connection formation and maturation. Dysregulation of these processes can result in improper neuronal connectivity, including neurodevelopmental disorders. The y-Protocadherins (y-Pcdhs) are involved in multiple aspects of neurodevelopment, including neuronal survival, dendrite arborization, and synapse development. The specific role of each individual y-Pcdh family member remains unclear.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Neurosciences
Shan Meltzer, Katelyn C. Boulanger, Anda M. Chirila, Emmanuella Osei-Asante, Michelle DeLisle, Qiyu Zhang, Brian T. Kalish, Aniqa Tasnim, Erica L. Huey, Leah C. Fuller, Erin K. Flaherty, Tom Maniatis, Andrew M. Garrett, Joshua A. Weiner, David D. Ginty
Summary: Light touch sensation begins with the activation of low-threshold mechanoreceptor (LTMR) endings and the transmission of their signals in the spinal cord and brainstem. The Pcdhg gene locus, encoding 22 cell-surface homophilic binding proteins, is crucial for normal behavioral reactivity to tactile stimuli in somatosensory neurons. Developmentally, different Pcdhg isoforms play roles in synapse formation and axonal branching of LTMR through neuron-neuron and neuron-glia interactions. Disruption of Pcdhg isoforms leads to fewer corticospinal synapses in the dorsal horn, highlighting their importance in the assembly of central mechanosensory circuitry.
Article
Hematology
Katherine C. Wood, Brittany G. Durgin, Heidi M. Schmidtt, Scott A. Hahn, Jeffrey J. Baust, Tim Bachman, Dario A. Vitturi, Samit Ghosh, Solomon F. Ofori-Acquah, Ana L. Mora, Mark T. Gladwin, Adam C. Straub