Article
Biotechnology & Applied Microbiology
Joan Sebastian Gallego-Murillo, Giulia Iacono, Luuk A. M. van der Wielen, Emile van den Akker, Marieke von Lindern, Sebastian Aljoscha Wahl
Summary: This study reports on the scale-up of cRBC production from static cultures to 3 L stirred tank bioreactors, and identifies the effect of operating conditions on the efficiency of the process.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Cell Biology
Claudia Bernecker, Maria Augusta R. B. F. Lima, Catalin D. Ciubotaru, Peter Schlenke, Isabel Dorn, Dan Cojoc
Summary: This study introduces a novel optical tweezer approach to measure the deformation and elasticity of single red blood cells, providing new insights into the biomechanical characterization of cultured red blood cells. The results show that red blood cells cultured with human platelet lysate exhibit similar features to native red blood cells under optical forces, while those cultured with plasma display different characteristics.
Article
Critical Care Medicine
Maria Paula Oliveira Pires, Maria Angelica Sorgini Peterlini, Amanda J. Ullman, Andrew C. Bulmer, Claire M. Rickard, Mavilde Luz Goncalves Pedreira
Summary: The study found that warmed RBCs had lower total Hb and haematocrit levels, as well as increased free Hb levels, haemolysis levels, and LDH activity. Pump infusion RBCs showed reduced total Hb and increased free Hb, haemolysis, and potassium levels compared with warmed RBCs. Manual infusion of warmed RBCs resulted in significantly reduced total Hb levels and increased LDH activity.
AUSTRALIAN CRITICAL CARE
(2021)
Article
Oncology
Vanessa Neef, Sven Koenig, Daniel Monden, Daniel Dubinski, Anika Benesch, Florian J. Raimann, Florian Piekarski, Michael W. Ronellenfitsch, Patrick N. Harter, Christian Senft, Patrick Meybohm, Elke Hattingen, Kai Zacharowski, Volker Seifert, Peter Baumgarten
Summary: Transfusion of red blood cells (RBC) in patients undergoing major elective cranial surgery is associated with increased morbidity, mortality, and prolonged hospital length of stay. This study identified risk factors for RBC transfusion in skull base and non-skull base meningioma patients, including preoperative ASA physical status score, tumor size, surgical time, and intraoperative blood loss. Blood loss due to large tumors or localization near large vessels are the main triggers for RBC transfusion in meningioma patients.
Article
Hematology
Jiusheng Deng, Moira Lancelot, Ryan Jajosky, Qiaomei Deng, Kristin Deeb, Natia Saakadze, Yongxing Gao, David Jaye, Senquan Liu, Sean R. Stowell, Linzhao Cheng, John D. Roback
Summary: The study demonstrated the in vivo properties of human iPSC-RBCs using a murine model, showing that iPSC-RBCs with specific characteristics can be generated through culture supplemented with human platelet lysate, and that drug-treated mice can enhance the survival of these cells in the circulatory system.
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Article
Physiology
Alice Dussouchaud, Julieta Jacob, Charles Secq, Jean-Marc Verbavatz, Martina Moras, Jerome Larghero, Claudio M. Fader, Mariano A. Ostuni, Sophie D. Lefevre
Summary: During human erythroblast differentiation, mitochondria are progressively cleared and lose contact with the endoplasmic reticulum (ER) and nucleus (NAM). The number of endosomes and multivesicular bodies (MVBs) involved in iron traffic and heme synthesis increases during differentiation, as well as autophagosomes. Glycogen accumulation is observed in the OrthoE stage.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Angelo D'Alessandro, Alkmini T. Anastasiadi, Vassilis L. Tzounakas, Travis Nemkov, Julie A. Reisz, Anastsios G. Kriebardis, James C. Zimring, Steven L. Spitalnik, Michael P. Busch
Summary: Red blood cells play a central role in oxygen transport in the human body. Recent advancements in omics technologies have revealed the complex proteome and metabolome of red blood cells, leading to a reevaluation of the mechanisms underlying RBC metabolism. These findings have also provided insights into changes that occur during refrigerated storage of RBCs in blood banks, which are crucial for life-saving transfusions worldwide.
Article
Biochemistry & Molecular Biology
Philippe Chadebech, Gwellaouen Bodivit, Gaetana Di Liberto, Alicia Jouard, Corinne Vasseur, France Pirenne, Pablo Bartolucci
Summary: In this study, senescence markers on AA RBCs associated with SCD patients' clinical status and their adhesion to the vessel wall protein thrombospondin were investigated. The results showed that modulators of RBC membrane channels can reproduce the adhesion of senescent RBCs after plasma co-incubations. Clinically modulating RBC adhesion to vascular wall proteins might be a promising avenue for the treatment of painful occlusive events in SCD.
Article
Biochemistry & Molecular Biology
Chien-Sung Tsai, Mei-Hua Hu, Yung-Chi Hsu, Go-Shine Huang
Summary: This study investigated the role of platelet TLR4 in transfusion reactions and found that blood mixing can upregulate platelet TLR4 and certain DAMPs, potentially leading to innate immune responses and transfusion reactions.
Review
Hematology
Eric A. Gehrie, Bipin N. Savani, Garrett S. Booth
Summary: Advancements in laboratory testing, pathogen reduction, and donor qualification have significantly reduced the risk of infection from blood transfusions. However, the prevention and recognition of hemolytic reactions, particularly due to donor-recipient incompatibility, have seen little recent progress. The article discusses the role of compatibility testing and transfusion practices in acute hemolysis, emphasizing clinical scenarios with increased risk and outlining initial clinical management for severe hemolytic reactions.
Article
Multidisciplinary Sciences
Ziya Isiksacan, Angelo D'Alessandro, Susan M. Wolf, David H. McKenna, Shannon N. Tessier, Erdem Kucukal, A. Aslihan Gokaltun, Nishaka William, Rebecca D. Sandlin, John Bischof, Narla Mohandas, Michael P. Busch, Caglar Elbuken, Umut A. Gurkan, Mehmet Toner, Jason P. Acker, Martin L. Yarmush, O. Berk Usta
Summary: Transfusion of red blood cells is a valuable and widespread treatment in modern medicine. Current practices in blood transfusion overlook the variability in stored red blood cell quality. It is necessary to rethink transfusion medicine workflows and utilize novel technologies for assessing red blood cell quality.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Medicine, General & Internal
Siti Zaleha S. Abdullah, Mohd Nazri Hassan, Marini Ramli, Marne Abdullah, Noor Haslina Mohd Noor
Summary: Red blood cell (RBC) alloimmunization is a significant complication of blood transfusion, with variation in prevalence among different patient populations. This study aimed to determine the prevalence and factors associated with RBC alloimmunization among chronic liver disease (CLD) patients. A total of 441 CLD patients were included, and the overall prevalence of RBC alloimmunization was 5.4%, with higher rates in females and patients with autoimmune hepatitis. The most common alloantibodies were from the Rh blood group. There was no significant factor associated with RBC alloimmunization among CLD patients. Therefore, it is recommended to provide Rh blood group phenotype matching for CLD patients requiring blood transfusions to prevent RBC alloimmunization.
Article
Environmental Sciences
Vaclav Tvrdy, Patricia Dias, Iveta Nejmanova, Alejandro Carazo, Eduard Jirkovsky, Jana Pourova, Jaka Fadraersada, Monika Moravcova, Lucija Peterlin Masic, Marija Sollner Dolenc, Premysl Mladenka
Summary: The human population is regularly exposed to bisphenols, which are potentially toxic. This study aimed to test the effects of 14 bisphenols on the cardiovascular system and compare their effects in different settings. Most of the tested bisphenols could relax rat aorta, but their potency varied significantly.
Article
Medicine, General & Internal
Jan A. Graw, Victoria Buenger, Lorenz A. Materne, Alexander Krannich, Felix Balzer, Roland C. E. Francis, Axel Pruss, Claudia D. Spies, Wolfgang M. Kuebler, Steffen Weber-Carstens, Mario Menk, Oliver Hunsicker
Summary: This study investigated the effects of different storage durations of PRBCs on ARDS patients. The results showed that prolonged storage of PRBCs did not increase mortality in adults with ARDS, but transfusion of long-term stored PRBCs was associated with prolonged dependence on RRT in critically ill patients with ARDS.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Sondos Zayed, Timothy K. Nguyen, Cindy Lin, Gabriel Boldt, Sushil Beriwal, Carien L. Creutzberg, Mitchell Kamrava, Lucas C. Mendez, Vikram Velker, Corinne Doll, Amandeep Taggar, Eric Leung, David P. D'Souza
Summary: This study aimed to develop consensus statements to guide PRBC transfusion practices in patients with cervical cancer receiving curative-intent RT with EBRT and brachytherapy, using an international Delphi consensus method. Through the consensus, a hemoglobin transfusion target of 9 g/dL or more and less than 12 g/dL was endorsed for patients who receive a PRBC transfusion.
Review
Biochemistry & Molecular Biology
Maria Zingariello, Fabrizio Martelli, Paola Verachi, Claudio Bardelli, Francesca Gobbo, Maria Mazzarini, Anna Rita Migliaccio
Article
Cardiac & Cardiovascular Systems
Valentina Spigoni, Federica Fantuzzi, Cecilia Carubbi, Giulia Pozzi, Elena Masselli, Giuliana Gobbi, Anna Solini, Riccardo C. Bonadonna, Alessandra Dei Cas
CARDIOVASCULAR DIABETOLOGY
(2020)
Editorial Material
Hematology
Anna Rita Migliaccio
Review
Biochemistry & Molecular Biology
Elena Masselli, Giulia Pozzi, Mauro Vaccarezza, Prisco Mirandola, Daniela Galli, Marco Vitale, Cecilia Carubbi, Giuliana Gobbi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Anatomy & Morphology
Maria Mazzarini, Mario Falchi, Daniele Bani, Anna Rita Migliaccio
Summary: Histology is the study of cell morphology within natural tissue environment. With advancements in microscopy techniques, we are able to better understand the relationships between cell shape and function, leading to further research in this field.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Review
Environmental Sciences
Valentina Presta, Luca Ambrosini, Cecilia Carubbi, Elena Masselli, Prisco Mirandola, Maria Luisa Arcari, Giuliana Gobbi, Marco Vitale
Summary: Maintaining hydration status is crucial for athletes, with a balance of fluid and salt intake being key. Natural mineral spring waters, rich in various salt compositions, may help meet the hydro-saline requirements, but there is limited scientific research in this area.
Review
Chemistry, Multidisciplinary
Daniela Galli, Cecilia Carubbi, Elena Masselli, Mauro Vaccarezza, Valentina Presta, Giulia Pozzi, Luca Ambrosini, Giuliana Gobbi, Marco Vitale, Prisco Mirandola
Summary: Reactive Oxygen Species (ROS) are harmful molecules produced naturally by cells. Aging impairs the antioxidant system, leading to age-related diseases. Exercise stimulates ROS production but also enhances the antioxidant defense, playing a role in maintaining the balance of the oxidant/antioxidant environment.
APPLIED SCIENCES-BASEL
(2021)
Article
Oncology
Francesca Palandri, Mario Tiribelli, Massimo Breccia, Daniela Bartoletti, Elena M. Elli, Giulia Benevolo, Bruno Martino, Francesco Cavazzini, Alessia Tieghi, Alessandra Iurlo, Elisabetta Abruzzese, Novella Pugliese, Gianni Binotto, Giovanni Caocci, Giuseppe Auteri, Daniele Cattaneo, Malgorzata M. Trawinska, Rossella Stella, Luigi Scaffidi, Nicola Polverelli, Giorgia Micucci, Elena Masselli, Monica Crugnola, Costanza Bosi, Florian H. Heidel, Roberto Latagliata, Fabrizio Pane, Antonio Cuneo, Mauro Krampera, Gianpietro Semenzato, Roberto M. Lemoli, Michele Cavo, Nicola Vianelli, Massimiliano Bonifacio, Giuseppe A. Palumbo
Summary: The study found that in MF patients who rechallenged with ruxolitinib after a short discontinuation, there were clinical improvements and potential survival advantages, but with a high rate of permanent discontinuation. Ruxolitinib rechallenge should be balanced against newer therapeutic possibilities.
Editorial Material
Medicine, General & Internal
Elena Masselli, Marco Vitale
Article
Oncology
Elena Masselli, Cecilia Carubbi, Giulia Pozzi, Antonio Percesepe, Rita Campanelli, Laura Villani, Giuliana Gobbi, Sabrina Bonomini, Giovanni Roti, Vittorio Rosti, Margherita Massa, Giovanni Barosi, Marco Vitale
Summary: The homozygosity for the rs1024611 SNP of the chemokine CCL2 is a high-risk variant associated with reduced survival in primary myelofibrosis (PMF). Additionally, ruxolitinib can effectively down-regulate CCR2 expression in PMF cells, providing a potential new therapeutic strategy for PMF patients.
Article
Biochemistry & Molecular Biology
Giulia Pozzi, Elena Masselli, Giuliana Gobbi, Prisco Mirandola, Luis Taborda-Barata, Luca Ampollini, Paolo Carbognani, Cristina Micheloni, Francesco Corazza, Daniela Galli, Cecilia Carubbi, Marco Vitale
Summary: The COVID-19 pandemic has affected nearly 190 million people worldwide with over 4 million confirmed deaths. Finding protective and therapeutic strategies is urgent, and H2S may help reduce the potential for SARS-CoV-2 to enter respiratory epithelial cells.
Article
Cell Biology
Giulia Pozzi, Giuliana Gobbi, Elena Masselli, Cecilia Carubbi, Valentina Presta, Luca Ambrosini, Marco Vitale, Prisco Mirandola
Summary: T cell-mediated adaptive immunity responds to non-self antigens and pathogens by activating and proliferating various T cell populations, with hydrogen sulfide playing a crucial regulatory role in this process.
Review
Cell Biology
Elena Masselli, Giulia Pozzi, Cecilia Carubbi, Marco Vitale
Summary: Myeloproliferative neoplasms are hematologic malignancies with a significant heritable component that can be affected by germline variants. Host genetic factors play a role in determining the phenotypic presentation, outcome, and response to therapy of MPNs. Understanding the genetic determinants of clonal hematopoiesis can provide insights into the development of hematologic neoplasms.
Article
Hematology
Francesca Arciprete, Paola Verachi, Fabrizio Martelli, Mauro Valeri, Manjola Balliu, Paola Guglielmelli, Alessandro Maria Vannucchi, Anna Rita Migliaccio, Maria Zingariello
Summary: Emperipolesis between neutrophils and megakaryocytes plays an important role in myelofibrosis. The challenge of studying this phenomenon by transmission electron microscopy has been overcome by the establishment of a user-friendly confocal microscopy method. Treatment with the inhibitor reparixin can reduce the emperipolesis between neutrophils and megakaryocytes. These findings identify neutrophil/megakaryocyte emperipolesis as the cellular interaction that links interleukin 8 to TGF-,B abnormalities in the pathobiology of marrow fibrosis.
EXPERIMENTAL HEMATOLOGY
(2023)
Article
Cell Biology
Giulia Pozzi, Valentina Presta, Elena Masselli, Giancarlo Condello, Samuele Cortellazzi, Maria Luisa Arcari, Cristina Micheloni, Marco Vitale, Giuliana Gobbi, Prisco Mirandola, Cecilia Carubbi
Summary: Reactive oxygen species (ROS) are key players in physiological processes and can affect myogenic differentiation. Protein kinase C epsilon (PKCe) is involved in muscle stem cell differentiation and skeletal muscle regeneration. PKCs have a role in redox biology and may regulate ROS production. Our study suggests that PKCe is a molecular link between redox homeostasis and myogenic differentiation. We found that PKCe interacts with Nrf2 to activate antioxidant signaling, preventing ROS accumulation and promoting myogenic differentiation.
