Article
Biochemical Research Methods
Xue Liu, Lingling Li, Guang-Rong Zhao
Summary: Using systems metabolic engineering strategies, a three-strain commensalistic Escherichia coli coculture was designed and developed for the de novo production of genistein. This platform offers a flexible and versatile approach for the production of flavonoids and holds promise for the production of complex natural products in engineered E. coli.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Energy & Fuels
Nitesh Kumar Mund, Yisong Liu, Shaolin Chen
Summary: Biofuels, such as biodiesel and alcohols, can be produced efficiently by microalgae and cyanobacteria through metabolic engineering and synthetic biology approaches, offering a sustainable alternative to fossil fuels. These microorganisms have the ability to convert CO2 and water into carbon-rich lipids, making them superior to traditional oil-seed crops. Cyanobacteria, in particular, have established genetic backgrounds and tolerance to foreign genes, making them promising hosts for biofuel synthesis from CO2, light, and water.
Review
Biochemistry & Molecular Biology
Kaitlyn Blatt-Janmaat, Yang Qu
Summary: The rapid growth of the global cannabis economy is driven by the medicinal properties of cannabis and its legal status in multiple countries. Besides THC, non-psychoactive phytocannabinoids are also rapidly developing and have the potential to become clinically useful drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Jing Zhang, Yue Yuan, Zhiwen Wang, Tao Chen
Summary: In this study, Halomonas bluephagenesis was engineered to efficiently produce Mevalonate (MVA). By screening MVA synthetases and introducing beneficial genes, along with the utilization of mixed carbon sources and non-oxidative glycolysis pathway, the MVA titer and yield were significantly improved. This study highlights the potential of H. bluephagenesis as a chassis for MVA production.
METABOLIC ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Xiaoxin Zhai, Jiaoqi Gao, Yunxia Li, Martin Grininger, Yongjin J. Zhou
Summary: Methanol is an ideal feedstock for chemical and biological manufacturing. Constructing an efficient cell factory is essential for producing complex compounds through methanol biotransformation. This study demonstrates that compartmentalizing fatty alcohol biosynthesis and methanol utilization in peroxisomes significantly improves fatty alcohol production in methylotrophic yeast.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Dongsoo Yang, Hyunmin Eun, Cindy Pricilia Surya Prabowo
Summary: Polyketides, a type of natural products, have versatile applications such as pharmaceuticals, nutraceuticals, and cosmetics. Aromatic polyketides, including type II and III polyketides, are important chemicals for human health, such as antibiotics and anticancer agents. However, they are mainly produced from challenging sources like soil bacteria or plants. To address this issue, metabolic engineering and synthetic biology have been used to efficiently engineer model microorganisms for enhanced production of aromatic polyketides. This review discusses the recent advancements and future prospects of metabolic engineering and synthetic biology strategies in the production of type II and type III polyketides in model microorganisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Tang-Lei Zhang, Hong-Wei Yu, Li-Dan Ye
Summary: This paper reviews the recent progress in the development of Y. lipolytica cell factories for terpenoid production, focusing on the advances in novel synbio tools and metabolic engineering strategies for enhanced terpenoid biosynthesis.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ye Zhang, Zihua Li, Yu Liu, Xuecong Cen, Dehua Liu, Zhen Chen
Summary: The economic viability of current bio-production systems is often limited by low productivity due to slow cell growth and low substrate uptake rate. Vibrio natriegens, a highly promising next-generation workhorse in the biotechnology industry, has been successfully engineered for heterologous production of 1,3-propanediol using systems metabolic engineering strategies. The final engineered strain can efficiently produce 1,3-PDO with high titer, yield, and productivity.
METABOLIC ENGINEERING
(2021)
Article
Agricultural Engineering
Raveendran Sindhu, Aravind Madhavan, K. B. Arun, Arivalagan Pugazhendhi, R. Reshmy, Mukesh Kumar Awasthi, Ranjna Sirohi, Ayon Tarafdar, Ashok Pandey, Parameswaran Binod
Summary: Research on the potential of biopolymers PHAs in replacing non-biodegradable plastics, including their synthesis in microorganisms, biodegradability, and comparable physico-chemical properties to synthetic plastics.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Hasnat Tariq, Saaim Asif, Anisa Andleeb, Christophe Hano, Bilal Haider Abbasi
Summary: Flavonoids, a heterogeneous family of plant polyphenolic compounds, have significant bioactivity in anti-cancer, antioxidant, anti-inflammatory, and anti-microbial activities. However, traditional approaches for their production have limitations. Genetic engineering tools offer promise for exploring and scaling up production of flavonoids by modifying biosynthetic pathways in plants and microbes.
Article
Microbiology
Mengyang Lei, Zetian Qiu, Leilei Guan, Zheng Xiang, Guang-Rong Zhao
Summary: In this study, a metabolic engineering approach was employed to produce Z,Z-farnesol (Z,Z-FOH) in Escherichia coli. By testing various enzymes and mutants, the optimal strain was able to achieve the highest reported titer of Z,Z-FOH in microbes. This work not only provides insights into the de novo biosynthesis of Z,Z-FOH in E. coli, but also lays a foundation for the development of synthetic E. coli cell factories for other cis-configuration terpenoids.
Review
Biotechnology & Applied Microbiology
Govinda R. Navale, Mahesh S. Dharne, Sandip S. Shinde
Summary: Isoprenoids, also known as terpenoids, are diverse organic compounds found abundantly in nature, with various applications in pharmaceutical, nutraceutical, and chemical industries. Recent advancements in metabolic engineering have enabled the production of isoprenoids in heterologous host systems like Escherichia coli and Saccharomyces cerevisiae. Challenges and strategies for scale-up and engineering of isoprenoids in heterologous host systems have been discussed in detail.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Feng Guo, Zhongxue Dai, Wenfang Peng, Shangjie Zhang, Jie Zhou, Jiangfeng Ma, Weiliang Dong, Fengxue Xin, Wenming Zhang, Min Jiang
Summary: The study demonstrates the application of rational design and gene regulation in engineeringP. pastoristo produce malic acid from sole methanol, achieving a significant increase in malic acid yield through optimization of accumulation modules and gene knockouts. The results provide important guidance and reference for metabolic engineering ofP. pastoristo produce value-added chemicals.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Sophie Vaud, Nicole Pearcy, Marko Hanzevacki, Alexander M. W. Van Hagen, Salah Abdelrazig, Laudina Safo, Muhammad Ehsaan, Magdalene Jonczyk, Thomas Millat, Sean Craig, Edward Spence, James Fothergill, Rajesh Reddy Bommareddy, Pierre-Yves Colin, Jamie Twycross, Paul A. Dalby, Nigel P. Minton, Christof M. Jaeger, Dong-Hyun Kim, Jianping Yu, Pin-Ching Maness, Sean Lynch, Carrie A. Eckert, Alex Conradie, Samantha J. Bryan
Summary: Ethylene is a widely used small hydrocarbon gas in the chemical industry, but its production contributes to significant CO2 emissions, highlighting the need for a sustainable alternative. Research has shown that a combination of genome-scale metabolic modeling, continuous fermentation, and protein evolution can accelerate the development of a high-efficiency ethylene-producing E. coli strain, resulting in a 49-fold increase in production.
METABOLIC ENGINEERING
(2021)
Article
Biochemical Research Methods
Dengwei Lei, Zetian Qiu, Jihua Wu, Bin Qiao, Jianjun Qiao, Guang-Rong Zhao
Summary: This study successfully improved the de novo production of nerol and borneol in E. coli by combining metabolic and plant monoterpene synthase engineering, achieving the highest reported titers of nerol and borneol in microbes to date.
ACS SYNTHETIC BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Sammy Pontrelli, Tsan-Yu Chiu, Ethan I. Lan, Frederic Y. -H. Chen, Peiching Chang, James C. Liao
METABOLIC ENGINEERING
(2018)
Article
Endocrinology & Metabolism
Sastia Prama Putri, Yasumune Nakayama, Claire Shen, Shingo Noguchi, Katsuaki Nitta, Takeshi Bamba, Sammy Pontrelli, James Liao, Eiichiro Fukusaki
Article
Multidisciplinary Sciences
Hong Yu, Xiaoqian Li, Fabienne Duchoud, Derrick S. Chuang, James C. Liao
NATURE COMMUNICATIONS
(2018)
Article
Biochemical Research Methods
William B. Black, Linyue Zhang, Cody Kamoku, James C. Liao, Han Li
ACS SYNTHETIC BIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Sammy Pontrelli, Riley C. B. Fricke, Sana Subhan Memon Sakurai, Sastia Prama Putri, Sorel Fitz-Gibbon, Matthew Chung, Hsin-Yi Wu, Yu-Ju Chen, Matteo Pellegrini, Eiichiro Fukusaki, James C. Liao
METABOLIC ENGINEERING
(2018)
Article
Biotechnology & Applied Microbiology
Chang-Ting Chen, Frederic Y. -H. Chen, Igor W. Bogorad, Tung-Yun Wu, Ruoxi Zhang, Abraxa S. Lee, James C. Liao
METABOLIC ENGINEERING
(2018)
Article
Multidisciplinary Sciences
Hong Yu, James C. Liao
NATURE COMMUNICATIONS
(2018)
Article
Biotechnology & Applied Microbiology
Katsuaki Nitta, Walter A. Lavina, Sammy Pontrelli, James C. Liao, Sastia P. Putri, Eiichiro Fukusakil
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2019)
Article
Biochemistry & Molecular Biology
Sammy Pontrelli, Riley C. B. Fricke, Shao Thing Teoh, Walter A. Lavina, Sastia Prama Putri, Sorel Fitz-Gibbon, Matthew Chung, Matteo Pellegrini, Eiichiro Fukusaki, James C. Liao
NATURE CHEMICAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Frederic Y-H Chen, Hsin-Wei Jung, Chao-Yin Tsuei, James C. Liao
Article
Biotechnology & Applied Microbiology
Derrick Shih-Wei Chuang, James C. Liao
Summary: This study revealed the important role of phosphoketolase (Xpk) in primary metabolism of cyanobacteria, particularly in energy regulation and dark conditions. Additionally, Xpk is involved in the pathway producing ATP from glycogen to acetate. Deletion of xpk leads to glucose secretion under dark, anaerobic conditions.
METABOLIC ENGINEERING
(2021)
Article
Chemistry, Physical
Shanshan Luo, Paul P. Lin, Liang-Yu Nieh, Guan-Bo Liao, Po-Wen Tang, Chi Chen, James C. Liao
Summary: Biological CO2 fixation is the most effective method for large-scale CO2 reduction, accounting for the majority of CO2 fixed on Earth. Cell-free CO2 fixation systems offer an alternative approach, allowing independent control of the fixation rate. By designing an oxygen-insensitive, self-replenishing CO2 fixation system with opto-sensing, the researchers achieved a sustained CO2-fixing rate comparable to or greater than photosynthetic or lithoautotrophic organisms.
Article
Cell Biology
Jing-You Guo, I-Ju Liu, Hsiu-Ting Lin, Mei-Jung Wang, Yu-Ling Chang, Shin-Chang Lin, Mei-Ying Liao, Wei-Chia Hsu, Yi-Ling Lin, James C. Liao, Han-Chung Wu
Summary: By using phage display technology and enzyme-linked immunosorbent assays, 36 enriched peptides were identified in the sera of two COVID-19 patients, with 4 motifs having consensus residues corresponding to two potential B-cell epitopes on SARS-CoV-2 viral proteins. These findings may aid in investigating COVID-19 pathogenesis and advancing the development of epitope-based serological diagnostics and vaccines.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Hsin-Chou Yang, Chun-houh Chen, Jen-Hung Wang, Hsiao-Chi Liao, Chih-Ting Yang, Chia-Wei Chen, Yin-Chun Lin, Chiun-How Kao, Mei-Yeh Jade Lu, James C. Liao
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biotechnology & Applied Microbiology
Hiroki Nishiguchi, James Liao, Hiroshi Shimizu, Fumio Matsuda
METABOLIC ENGINEERING COMMUNICATIONS
(2020)