Microbial heterogeneity affects bioprocess robustness: Dynamic single-cell analysis contributes to understanding of microbial populations

Evolutionary engineering ofSaccharomyces cerevisiaefor enhanced tolerance to hydrolysates of lignocellulosic biomass

(2013) María P. Almario et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recombineering to homogeneity: extension of multiplex recombineering to large-scale genome editing

(2013) Nanette R. Boyle et al.   Biotechnology Journal

Design of growth-dependent biosensors based on destabilized GFP for the detection of physiological behavior ofEscherichia coliin heterogeneous bioreactors

(2013) Shanshan Han et al.   BIOTECHNOLOGY PROGRESS

A Decade of Riboswitches

(2013) Alexander Serganov et al.   CELL

Real-time monitoring of cell viability and cell density on the basis of a three dimensional optical reflectance method (3D-ORM): investigation of the effect of sub-lethal and lethal injuries

(2013) Alison Brognaux et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Simultaneous Quantitative Live Cell Imaging of Multiple FRET-Based Biosensors

(2013) Andrew Woehler   PLoS One

Microtools for single-cell analysis in biopharmaceutical development and manufacturing

(2013) Kerry Routenberg Love et al.   TRENDS IN BIOTECHNOLOGY

Coupling of biokinetic and population balance models to account for biological heterogeneity in bioreactors

(2012) Jérôme Morchain et al.   AICHE JOURNAL

Bioreactor monitoring with spectroscopy and chemometrics: a review

(2012) N. D. Lourenço et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Isolated Microbial Single Cells and Resulting Micropopulations Grow Faster in Controlled Environments

(2012) Christian Dusny et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Physiological heterogeneity of Pseudomonas taetrolens during lactobionic acid production

(2012) Saúl Alonso et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Characterization of Saccharomyces cerevisiae promoters for heterologous gene expression in Kluyveromyces marxianus

(2012) Ki-Sung Lee et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Flow following sensor particles—Validation and macro-mixing analysis in a stirred fermentation vessel with a highly viscous substrate

(2012) S. Reinecke et al.   BIOCHEMICAL ENGINEERING JOURNAL

Cell mass and cell cycle dynamics of an asynchronous budding yeast population: Experimental observations, flow cytometry data analysis, and multi-scale modeling

(2012) Rita Lencastre Fernandes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Genome-wide overexpression screen for sodium acetate resistance in Saccharomyces cerevisiae

(2012) Pedro V. Peña et al.   JOURNAL OF BIOTECHNOLOGY

Visualizing evolution in real time to determine the molecular mechanisms of n-butanol tolerance in Escherichia coli

(2012) Luis H. Reyes et al.   METABOLIC ENGINEERING

Physiological heterogeneities in microbial populations and implications for physical stress tolerance

(2012) Magnus Carlquist et al.   Microbial Cell Factories

Culture History and Population Heterogeneity as Determinants of Bacterial Adaptation: the Adaptomics of a Single Environmental Transition

(2012) B. Ryall et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Tandem fluorescent protein timers for in vivo analysis of protein dynamics

(2012) Anton Khmelinskii et al.   NATURE BIOTECHNOLOGY

Quantitative imaging using genetically encoded sensors for small molecules in plants

(2012) Sakiko Okumoto   PLANT JOURNAL

Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity

(2012) Kerry Routenberg Love et al.   PLoS One

Single-Cell Analysis in Biotechnology, Systems Biology, and Biocatalysis

(2012) Frederik S.O. Fritzsch et al.   Annual Review of Chemical and Biomolecular Engineering

Mapping the Environmental Fitness Landscape of a Synthetic Gene Circuit

(2012) Dmitry Nevozhay et al.   PLoS Computational Biology

Visualizing evolution in real-time method for strain engineering

(2012) Luis H. Reyes et al.   Frontiers in Microbiology

A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level

(2012) Stephan Binder et al.   GENOME BIOLOGY

Life, Death, and In-Between: Meanings and Methods in Microbiology

(2011) Hazel M. Davey   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Experimental methods and modeling techniques for description of cell population heterogeneity

(2011) R. Lencastre Fernandes et al.   BIOTECHNOLOGY ADVANCES

Programming microbial population dynamics by engineered cell-cell communication

(2011) Hao Song et al.   Biotechnology Journal

Green fluorescent protein (GFP) leakage from microbial biosensors provides useful information for the evaluation of the scale-down effect

(2011) Frank Delvigne et al.   Biotechnology Journal

Bioprocess monitoring by marker gene analysis

(2011) Thomas Schweder   Biotechnology Journal

Single-cell protein analysis

(2011) Meiye Wu et al.   CURRENT OPINION IN BIOTECHNOLOGY

Effect of post-induction substrate oscillation on recombinant alkaline phosphatase production expressed in Escherichia coli

(2011) Mohammadhadi Jazini et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools

(2011) Florian David et al.   Microbial Cell Factories

Genome-wide analysis of yeast stress survival and tolerance acquisition to analyze the central trade-off between growth rate and cellular robustness

(2011) Anna Zakrzewska et al.   MOLECULAR BIOLOGY OF THE CELL

Origin and analysis of microbial population heterogeneity in bioprocesses

(2010) Susann Müller et al.   CURRENT OPINION IN BIOTECHNOLOGY

Single-cell tracking with a reversing flow cytometer

(2010) Greg Sitton et al.   CYTOMETRY PART A

Viability states of bacteria-Specific mechanisms of selected probes

(2010) Heike Sträuber et al.   CYTOMETRY PART A

Red but not dead? Membranes of stressed Saccharomyces cerevisiae are permeable to propidium iodide

(2010) H. M. Davey et al.   ENVIRONMENTAL MICROBIOLOGY

The role of physiological heterogeneity in microbial population behavior

(2010) Mary E Lidstrom et al.   Nature Chemical Biology

A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

(2010) Carina Banning et al.   PLoS One

Microfluidic Confinement of Single Cells of Bacteria in Small Volumes Initiates High-Density Behavior of Quorum Sensing and Growth and Reveals Its Variability

(2009) James Q. Boedicker et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Quantitative Time-Lapse Fluorescence Microscopy in Single Cells

(2009) Dale Muzzey et al.   Annual Review of Cell and Developmental Biology

Application of flow cytometry to industrial microbial bioprocesses

(2009) Mario Díaz et al.   BIOCHEMICAL ENGINEERING JOURNAL

A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring

(2009) Tim Höpfner et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Rapid strain improvement through optimized evolution in the cytostat

(2009) Alan Gilbert et al.   BIOTECHNOLOGY AND BIOENGINEERING

Genome-scale analysis of library sorting (GALibSo): Isolation of secretion enhancing factors for recombinant protein production inPichia pastoris

(2009) Gerhard Stadlmayr et al.   BIOTECHNOLOGY AND BIOENGINEERING

The fluorescent protein palette: tools for cellular imaging

(2009) Richard N. Day et al.   CHEMICAL SOCIETY REVIEWS

Exploitation of GFP fusion proteins and stress avoidance as a generic strategy for the production of high-quality recombinant proteins

(2009) Yanina Sevastsyanovich et al.   FEMS MICROBIOLOGY LETTERS

Bioreactor mixing efficiency modulates the activity of a prpoS::GFP reporter gene in E. coli

(2009) Frank Delvigne et al.   Microbial Cell Factories

Synthetic control of a fitness tradeoff in yeast nitrogen metabolism

(2009) Travis S Bayer et al.   Journal of Biological Engineering

Bistability, Epigenetics, and Bet-Hedging in Bacteria

(2008) Jan-Willem Veening et al.   Annual Review of Microbiology

Development and Application of Flow-Cytometric Techniques for Analyzing and Sorting Endospore-Forming Clostridia

(2008) B. P. Tracy et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Studies related to antibody fragment (Fab) production inEscherichia coliW3110 fed-batch fermentation processes using multiparameter flow cytometry

(2008) Andrew Want et al.   CYTOMETRY PART A

Analysis and modeling of growing budding yeast populations at the single cell level

(2008) Danilo Porro et al.   CYTOMETRY PART A

Stochastic switching as a survival strategy in fluctuating environments

(2008) Murat Acar et al.   NATURE GENETICS

Memory in Microbes: Quantifying History-Dependent Behavior in a Bacterium

(2008) Denise M. Wolf et al.   PLoS One

An Artificial Aptazyme-Based Riboswitch and its Cascading System inE. coli

(2007) Atsushi Ogawa et al.   CHEMBIOCHEM