Article
Chemistry, Analytical
HyeonSeok Shin, Youngmin Park, Kyunggeun Ahn, Sungsoo Kim
Summary: This study developed an algorithm that accurately predicts high-intensity peaks in peptide fragmentation spectra, which is crucial for peptide identification and selective monitoring.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
HyeonSeok Shin, Youngmin Park, Kyunggeun Ahn, Sungsoo Kim
Summary: In this study, an algorithm was developed to more accurately predict high-intensity peaks in peptide fragmentation spectra for improved peptide identification. The model, trained using different neural network architectures, showed better performance compared to previous machine learning-based prediction algorithms. The algorithm is particularly advantageous for selective/multiple reaction monitoring applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Shiyu Sun, Wanshan Liu, Jing Yang, Hang Wang, Kun Qian
Summary: This study investigated cation adduction and fragmentation of small metabolites using a combination of theoretical and experimental approaches, establishing rules and successfully controlling fragmentation to enhance metabolite identification and profiling. This work provides new insights for advanced analysis of small metabolites in mass spectrometry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Kim Greis, Carla Kirschbaum, Martin I. Taccone, Michael Goetze, Sandy Gewinner, Wieland Schoellkopf, Gerard Meijer, Gert von Helden, Kevin Pagel
Summary: mRNA-based vaccines have become crucial during the COVID-19 pandemic. This study investigates the mechanism of backbone cleavage of RNA strands and the tautomerization of cytosine in the gas phase using experimental and theoretical approaches. The findings have important implications for the development and analysis of mRNA vaccines.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemical Research Methods
Shardrack O. Asare, Kimberly R. Dean, Bert C. Lynn
Summary: Lignin and lignans are natural products found in plant cell walls, with research historically focusing on lignin degradation techniques and the interesting biological activities of lignans. Studies have shown that both lignin and lignan dimers can possess multiple chiral centers, leading to observations of enhanced activity in one diastereomer of a pair. Tandem mass spectrometry and HPLC can be used to differentiate diastereomer pairs in these series.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Biochemical Research Methods
Sven Heiles
Summary: Metabolomics and lipidomics are driving forces in the omics era, with tandem mass spectrometry methods playing a crucial role in characterizing the structural details of metabolites and lipids. Recent advancements in alternative tandem MS methodologies aim to provide full structural characterization of these compounds. The review critically examines the principles, capabilities, drawbacks, and initial applications of these advanced tandem mass spectrometry strategies in the fields of metabolomics and lipidomics.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Robert S. Plumb, Thomas McDonald, Paul D. Rainville, Jason Hill, Lee A. Gethings, Kelly A. Johnson, Ian D. Wilson
Summary: Vacuum jacketed column (VJC) technology can significantly improve the performance of UHPLC systems, enhancing chromatographic separation and increasing detection capabilities for metabolites. Using the VJC system can reduce peak tailing, increase peak capacity, and enhance the intensity and response of MS peaks.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Ceramics
Xiaodong Wang, Zhiyun Han, Yilei Yu, Zhaoxiu Jiang, Guangfa Gao
Summary: In this study, commonly used ceramics for ballistic protection were compared, and it was found that silicon carbide provides the best protection performance while boron carbide provides the worst. Additionally, the angles of ceramic cones formed by different ceramic plates under the same impact load were also different.
INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Yan Ma, Yang Cao, Xiaocui Song, Yuanying Zhang, Jing Li, Yankai Wang, Xiaoqing Wu, Xiangbing Qi
Summary: A software named BAFinder was developed to improve the identification of unknown bile acids. It covers a wide variety of bile acid structures and utilizes characteristic product ions and neutral losses for annotation. The successful analysis of human samples demonstrated the utility of BAFinder.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Cecilia Jimenez-Sanchez, Fabian Pedregosa, Isabel Borras-Linares, Jesus Lozano-Sanchez, Antonio Segura-Carretero
Summary: This study examined the phytochemical profiles of different asparagus varieties, finding significant differences between the triguero varieties (especially the improved HT-801 and its parent verde-morado) and two commercial green asparagus varieties. Specific compounds unique to the triguero varieties were identified, which could potentially be used for differentiation and revalorization of these asparagus cultivars.
Article
Chemistry, Applied
Junqiao Wang, Jing Zhao, Shaoping Nie, Mingyong Xie, Shaoping Li
Summary: The study successfully improved the ionization efficiency of glycans using on-target derivatization with 3-AQ/CHCA matrix in MALDI TOF MS, while enhancing the generation of characteristic fragment ions for more efficient structural interpretation.
FOOD HYDROCOLLOIDS
(2022)
Article
Food Science & Technology
Tehmina Anjum, Wajiha Iram, Mazhar Iqbal, Mateen Abbas, Waheed Akram, Guihua Li
Summary: The aqueous extracts of Mentha arvensis leaves show significant potential in degrading aflatoxin B1 and B2, with optimized conditions resulting in high degradation rates and low-toxicity products.
Editorial Material
Biochemistry & Molecular Biology
Rafal Franski, Monika Beszterda-Buszczak
Summary: Villalva et al. evaluated the potential of yarrow extract as a treatment for H. pylori infection. They used the agar-well diffusion bioassay to test the extract's antimicrobial activity. The extract was fractionated into two fractions containing polar phenolic compounds and monoterpenes and sesquiterpenes, respectively. The phenolic compounds were identified using HPLC-ESIMS, but there were some disputed product ions.
Article
Metallurgy & Metallurgical Engineering
Hong-yi Li, Xin-mian Chen, Dan-qing Li, Yun Guo, Jiang Diao, Bing Xie
Summary: A quick, efficient and sensitive valence analysis method of vanadium(V) was developed using HPLC to separate and quantify EDTA-complexed V(III), V(IV) and V(V) ions. The method can be applied to analyzing hazardous wastes, providing important guidance for the treatment of such wastes.
TRANSACTIONS OF NONFERROUS METALS SOCIETY OF CHINA
(2021)
Article
Biochemistry & Molecular Biology
Hend Zedan, Kousuke Morimura, Amr Elguoshy, Tadashi Yamamoto, Shunji Natsuka
Summary: Urine is an excellent biological fluid for biomarker discovery, reflecting both systemic and urogenital physiology. Thoroughly analyzing the N-glycome in urine has been challenging due to the low abundance of glycans attached to glycoproteins. This study successfully identified and quantified 109 N-glycans in urine, with approximately 50% coming from the kidney and urinary tract. Additionally, age/sex were found to correlate with the relative abundances of urinary N-glycome.
Article
Agriculture, Multidisciplinary
Mariana Manzoni Maroneze, Belen Caballero-Guerrero, Leila Queiroz Zepka, Eduardo Jacob-Lopes, Antonio Perez-Galvez, Maria Roca
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Chemistry, Applied
Antonio Perez-galvez, Isabel Viera, Itziar Benito, Maria Roca
Article
Chemistry, Applied
Yue Shi, Guangjing Chen, Kewei Chen, Xuhui Chen, Qingyue Hong, Jianquan Kan
Summary: Heat pump drying (HPD) was found to be the most suitable method for drying star anise, maintaining its quality with HPD-B sample showing the highest comprehensive score. Microwave drying had the shortest drying time, while HPD samples had higher volatile oil and trans-anethole content.
Article
Food Science & Technology
Zhirong Wang, Tao Zhong, Kewei Chen, Muying Du, Guangjing Chen, Xuhui Chen, Kaituo Wang, Zsolt Zalan, Krisztina Takacs, Jianquan Kan
Summary: The research demonstrated that VOCs produced by Pseudomonas fluorescens ZX have a significant inhibitory effect on blue mold disease caused by Penicillium italicum in postharvest citrus fruits, by inhibiting mycelial growth and conidial germination of the pathogen. Organic acids and sulfur compounds were identified as the key active components of VOCs, with dimethyl disulfide (DMDS) and dimethyl trisulfide (DMTS) showing the highest antifungal activity.
Article
Biochemistry & Molecular Biology
Antonio Perez-Galvez, Maria Visitacion Calvo, Josefa Aguayo-Maldonado, Javier Fontecha
Summary: This study investigates the digestion of milk lipids, focusing on the distribution of digested triacylglycerols in the micellar fraction, revealing differences in lipid profiles based on lactation period and processing stage. The isolation of micellar phase and chromatographic techniques are used to determine bioaccessible lipids, providing valuable insights for lipid absorption.
Article
Agriculture, Multidisciplinary
Isabel Viera, Marta Herrera, Maria Roca
Summary: This study investigated the bioaccessibility of chlorophyll and found that both chlorophyll polarity and food matrix can affect its bioavailability. Surprisingly, the application of in vitro standardized protocol was shown to impact chlorophyll bioaccessibility.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Ecology
Maria Cecilia Chiale, Miguel A. Rendon, Sophie Labaude, Anne-Sophie Deville, Juan Garrido-Fernandez, Antonio Perez-Galvez, Araceli Garrido, Manuel Rendon-Martos, Arnaud Bechet, Juan A. Amat
Summary: Greater flamingos use cosmetic coloration by spreading pigmented uropygial secretions over their feathers to make them redder. Experimental exposure to sunlight resulted in fading of feather color, indicating the need for regular application of makeup to maintain colorful plumage.
ECOLOGY AND EVOLUTION
(2021)
Review
Biochemistry & Molecular Biology
Maria Roca, Antonio Perez-Galvez
Summary: Chlorophylls and carotenoids are recognized as added-value ingredients due to their antioxidant properties present in daily ingested foods, with new extraction and analysis methods being more potent, precise, and accurate. However, their application necessitates a deeper understanding of the technical and biological context.
Article
Agriculture, Multidisciplinary
Carlos Quiles, Isabel Viera, Maria Roca
Summary: This study investigates the differences in chlorophyll content among various olive fruit varieties during their ripening process, revealing the roles and pathways of enzymes involved in chlorophyll biosynthesis and degradation in olive fruits.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Applied
Isabel Viera, Marta Herrera, Maria Roca
Summary: This research analyzed the chlorophyll bioaccessibility of ten commercial foods and found a correlation between chlorophyll degradation during digestion and the salt content of the food. Regardless of the nutritional composition or chlorophyll content, the chlorophyll profile after digestion consisted of 90% pheophytins and 10% chlorophylls.
Article
Biochemistry & Molecular Biology
Marta Herrera, Isabel Viera, Maria Roca
Summary: The quality of green teas is measured by the green color, which can be influenced by variables such as cultivation and processing methods. This study analyzed the chlorophyll profiles of different green tea varieties and found unique chlorophyll compounds in each variety. The total chlorophyll content and a/b ratio can be used to statistically differentiate shadow teas and light-regimen green teas. Matcha tea was found to have a higher proportion of chlorophylls a and b, justifying its higher quality and price. Other chlorophyll metabolites were indicative of processing and storage conditions.
Article
Chemistry, Applied
Antonio Perez-Galvez, Maria Roca
Summary: Consumers' demand for higher food quality and safety has prompted food legislative organizations to develop regulations that ensure quality and safety criteria by understanding the composition of food. This is the background for green natural food colorants and the new category of green coloring foodstuffs. Through targeted metabolomics and advanced software, we have identified and studied the comprehensive chlorophyll composition in commercial samples of these colorants. Our findings include the discovery of seven new chlorophylls and eight previously undescribed chlorophylls, providing valuable insights for the field of chlorophyll chemistry. Furthermore, we have elucidated the chemical reactions involved in the manufacturing process of green food colorants and proposed a complete pathway for the occurrence of the chlorophylls they contain.
Article
Food Science & Technology
Victoria Martinez-Sanchez, M. Visitacion Calvo, I. Viera, J. Giron-Calle, J. Fontecha, Antonio Perez-Galvez
Summary: The milk fat globule membrane (MFGM), with its unique composition and arrangement of lipids, provides both infants and adults with various health benefits. Research has shown a fusion process between MFGM and the cellular membrane of differentiated Caco-2 cells, supported by the use of fluorochromes associated with lipid derivatives. By incubating MFGM with Caco-2 cells in the presence of fusogenic agents or compounds that block other MFGM interaction pathways, the mechanism of this interaction has been explored. Confocal fluorescence microscopy provided visual evidence of the fusion process, and analysis of the lipid profile of cells after their interaction with MFGM indicated a metabolic rearrangement of lipids resulting in the accumulation of triacylglycerols.
FOOD RESEARCH INTERNATIONAL
(2023)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)