Article
Cell Biology
Aujan Mehregan, Goli Ardestani, Hiroki Akizawa, Ingrid Carvacho, Rafael Fissore
Summary: By knocking out TRPV3 and Ca(V)3.2, we demonstrated their essential roles in completing Ca2+ store filling in mouse oocytes and eggs, as well as in initiating and maintaining periodic oscillations during mammalian fertilization.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Narumi Ogonuki, Hirohisa Kyogoku, Toshiaki Hino, Yuki Osawa, Yasuhiro Fujiwara, Kimiko Inoue, Tetsuo Kunieda, Seiya Mizuno, Hiroyuki Tateno, Fumihiro Sugiyama, Tomoya S. Kitajima, Atsuo Ogura
Summary: The efficiency of spermatocyte injection can be improved greatly by reducing the size of the recipient oocytes, and this technique shows potential for treating nonobstructive azoospermia caused by meiotic failure.
Review
Cell Biology
Ingrid Fliniaux, Guillaume Marchand, Caroline Molinaro, Mathieu Decloquement, Alain Martoriati, Matthieu Marin, Jean-Francois Bodart, Anne Harduin-Lepers, Katia Cailliau
Summary: Sialic acids are a group of monosaccharides with unique physicochemical properties that modulate the biological functions of molecules they are associated with. They play a role in various stages of the reproductive process, particularly in the formation and binding of gametes. Sialoglycoproteins form a specific coat on the surface of gametes, and marine species oocytes have specific polysialylated chains. Spermatozoa undergo crucial changes in sialic acid in the female reproductive tract, facilitating migration, survival, and binding with the oocyte.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Katherine A. Maniates, Andrew Singson
Summary: Complementary forward and reverse genetic approaches have led to a recent surge in the discovery of fertilization genes in various model systems. The number of genetically validated gamete surface molecules has more than doubled in the past few years, with all sperm fertilization genes encoding transmembrane or secreted molecules. However, the discovery of genes encoding oocyte molecules has lagged behind. This review discusses potential experimental biases and biological reasons that may have hindered the identification of egg fertilization genes, as well as current strategies to overcome these obstacles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Meina He, Tuo Zhang, Yi Yang, Chao Wang
Summary: Meiosis is essential for sexual reproduction, with oocytes in female mammals beginning meiosis before birth and sustaining it through dictyate stage before ovulation. Fine-tuning of meiotic progression is crucial for fertility, as any disruption can lead to oogenesis failure and impact overall reproductive health. Understanding the regulatory network of oocyte meiosis is key to comprehending mammalian reproduction and the consequences of misregulation on infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Obstetrics & Gynecology
Giovanni Coticchio, Andrea Borini, Carlotta Zaca, Evangelos Makrakis, Ioannis Sfontouris
Summary: With the introduction of time lapse technology and artificial intelligence, the precise timing, morphology, and geometry of fertilization events can provide clues for predicting the fate of the embryo, which is crucial in assisted reproduction.
HUMAN REPRODUCTION
(2022)
Article
Cell Biology
Meng Wang, Lixia Zhu, Chang Liu, Hui He, Cheng Wang, Chenxi Xing, Jinming Liu, Liu Yang, Qingsong Xi, Zhou Li, Lei Jin
Summary: The study demonstrates that a new AOA method combining cycloheximide and ionomycin significantly increases fertilization rates for most TFF patients, potentially offering therapeutic benefits for couples experiencing severe fertilization deficiencies even after conventional AOA treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Rebecca L. Krisher
Summary: This review examines the current use of in vitro oocyte maturation in clinical settings, including its different definitions in practice. It discusses the role of in vitro oocyte maturation in patient care and the challenges for implementation, as well as exploring its efficiency and safety. The review also discusses the role of in vitro oocyte maturation in oncofertility and provides an outlook for its future in clinical settings.
BIOLOGY OF REPRODUCTION
(2022)
Article
Multidisciplinary Sciences
Kaori Nozawa, Zian Liao, Yuhkoh Satouh, Ting Geng, Masahito Ikawa, Diana Monsivais, Martin M. Matzuk
Summary: Wee1-like protein kinase 2 (WEE2) is an oocyte-specific protein tyrosine kinase involved in the regulation of oocyte meiotic arrest. Knockout mouse models were developed to test the impact of Wee2 loss-of-function on female fertility. While a frameshift mutation had no effect, a larger deletion resulted in reduced litter size in homozygous Wee2 female knockout mice. Overall, loss of Wee2 had only minor effects on fertility in mice, suggesting alternative approaches should be considered for contraceptive development.
Review
Plant Sciences
Wei Deng, Yun Ling Xie, Hui Qiao Tian, Xue Yi Zhu
Summary: This article discusses the early changes that occur in the fused egg of higher plants before the fusion of the nuclei of male and female gametes, especially the unknown egg cell proteins responsible for sperm-egg recognition, attachment, and fusion.
CRITICAL REVIEWS IN PLANT SCIENCES
(2023)
Article
Biology
Lingli Huang, Wenqing Li, Xingxing Dai, Shuai Zhao, Bo Xu, Fengsong Wang, Ren-Tao Jin, Lihua Luo, Limin Wu, Xue Jiang, Yu Cheng, Jiaqi Zou, Caoling Xu, Xianhong Tong, Heng-Yu Fan, Han Zhao, Jianqiang Bao
Summary: This study identified and characterized novel biallelic variants in MAD2L1BP responsible for oocyte maturation arrest at MI, providing new therapeutic avenues for female primary infertility.
Article
Cell Biology
Virginia Savy, Paula Stein, Min Shi, Carmen J. Williams
Summary: The study found that while there were subtle differences in calcium signaling in eggs following superovulation during parthenogenetic activation, there was no impact on the physiological calcium signaling at fertilization. This suggests that superovulation does not disrupt normal calcium signaling during fertilization, supporting its use for both clinical and experimental purposes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Andrei Kouranov, Charles Armstrong, Ashok Shrawat, Vladimir Sidorov, Scott Huesgen, Bryce Lemke, Timothy Boyle, Michelle Gasper, Richard Lawrence, Samuel Yang
Summary: This study demonstrates the successful use of the CRISPR Cas12a system to achieve targeted chromosomal crossovers (CO) in hybrid maize. The experiments showed that stable and heritable targeted CO can be produced at a higher rate than natural CO. Molecular characterization revealed that these targeted CO were driven by non-homologous end joining (NHEJ) or HDR repair pathways during the mitotic cell cycle. These findings represent a step towards simplifying the creation of targeted genome combinations in progeny and accelerating breeding using RNA-guided nuclease technology.
COMMUNICATIONS BIOLOGY
(2022)
Article
Obstetrics & Gynecology
Marga Esbert, Andrew Carmody, Agustin Ballesteros, Emre Seli, Richard T. Scott
Summary: This study found that activation and cleavage can occur in unfertilized oocytes after failure to fertilize. However, exposure to Ca.I A23187 does not increase the prevalence of activation. These embryos arrest before reaching the blastocyst stage and have no reproductive potential.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Reproductive Biology
Kyungjun Uh, Alayna Hay, Paula Chen, Emily Reese, Kiho Lee
Summary: Artificial oocyte activation is essential for the application of Assisted Reproductive Technology, and the ability to control the level of cytoplasmic zinc can facilitate the oocyte activation process.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
Yi-Liang Miao, Andres Gambini, Yingpei Zhang, Elizabeth Padilla-Banks, Wendy N. Jefferson, Miranda L. Bernhardt, Weichun Huang, Leping Li, Carmen J. Williams
BIOLOGY OF REPRODUCTION
(2018)
Article
Agriculture, Dairy & Animal Science
Christopher W. Tubbs, Caitlin E. McDonough
ANNUAL REVIEW OF ANIMAL BIOSCIENCES, VOL 6
(2018)
Article
Multidisciplinary Sciences
Miranda L. Bernhardt, Paula Stein, Ingrid Carvacho, Christopher Krapp, Goli Ardestani, Aujan Mehregan, David M. Umbach, Marisa S. Bartolomei, Rafael A. Fissore, Carmen J. Williams
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Toxicology
Pei-Hsuan Hung, Laura S. Van Winkle, Carmen J. Williams, Patricia A. Hunt, Catherine A. VandeVoort
TOXICOLOGICAL SCIENCES
(2019)
Correction
Ecology
Julia D. Monk, Erin Giglio, Ambika Kamath, Max R. Lambert, Caitlin E. McDonough
NATURE ECOLOGY & EVOLUTION
(2020)
Review
Ecology
Julia D. Monk, Erin Giglio, Ambika Kamath, Max R. Lambert, Caitlin E. McDonough
NATURE ECOLOGY & EVOLUTION
(2019)
Article
Biochemistry & Molecular Biology
Caitlin E. McDonough, Miranda L. Bernhardt, Carmen J. Williams
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)
Editorial Material
Ecology
Ambika Kamath, Caitlin E. McDonough, Julia D. Monk, Max R. Lambert, Erin Giglio
NATURE ECOLOGY & EVOLUTION
(2020)
Article
Biology
Erin L. McCullough, Caitlin E. McDonough, Scott Pitnick, Steve Dorus
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2020)
Article
Genetics & Heredity
Caitlin E. McDonough-Goldstein, Kirill Borziak, Scott Pitnick, Steve Dorus
Summary: The study of gene expression in the Drosophila melanogaster FRT revealed significant differences between tissues, but postmating responses showed a coordinated systems-level functional response.
G3-GENES GENOMES GENETICS
(2021)
Article
Biology
Caitlin E. McDonough-Goldstein, Scott Pitnick, Steve Dorus
Summary: Reproductive traits that affect female remating and competitive fertilization rapidly evolve in response to sexual selection. In the fruit fly, a structural plug formed in the female reproductive tract influences sperm entry into storage and female remating. Glandular tissues and female proteins play essential roles in the formation and processing of the plug.
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2022)
Article
Multidisciplinary Sciences
Mariana I. Giassetti, Deqiang Miao, Nathan C. Law, Melissa J. Oatley, Julie Park, LeeLa D. Robinson, Lisette A. Maddison, Miranda L. Bernhardt, Jon M. Oatley
Summary: The molecule ARRDC5 is identified as an essential regulator of mammalian spermatogenesis, and its deficiency leads to male infertility. This finding implicates ARRDC5 as a potential target for male contraception.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Caitlin E. McDonough-Goldstein, Emma Whittington, Erin L. McCullough, Sharleen M. Buel, Scott Erdman, Scott Pitnick, Steve Dorus
Summary: This study investigates the proteomic composition of the female reproductive tract (FRT) tissue and luminal fluid before and after mating in Drosophila melanogaster, revealing distinct characteristics between the fluid proteome and tissue proteome. The fluid proteome is encoded by genes with higher FRT gene expression levels and tissue specificity, enriched for metabolic functions, and exhibits significant postmating compositional changes. These findings enhance our understanding of secretion mechanisms in the FRT, provide insights into female contributions to reproductive interactions, and underscore the importance of proteomic approaches in studying the dynamics of the FRT environment.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Meeting Abstract
Critical Care Medicine
W. Lin, K. M. Gowdy, J. H. Madenspacher, M. Lyons-Cohen, H. Nakano, D. N. Cook, R. L. Zemans, J. P. Mizgerd, C. J. Williams, M. B. Fessler
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2018)
